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    Bio-Techne corporation mouse anti cd68
    A Immunofluorescence imaging demonstrating migration of <t>CD68+</t> macrophages cultured from control (MΦ con ) and SmoKO (MΦ SmoKO ) mice towards mouse gastric organoids. Co-cultures were treated with vehicle or gastrin. B Macrophage migration within the co-cultures was quantified by forward migration index (FMI). * P < 0.001 compared to vehicle, n = 6 individual co-culture experiments. Data are shown as the mean ± SEM.
    Mouse Anti Cd68, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti cd68/product/Bio-Techne corporation
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti cd68 - by Bioz Stars, 2024-06
    95/100 stars
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    Standard format Plasmid sent in bacteria as agar stab
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    A Immunofluorescence imaging demonstrating migration of CD68+ macrophages cultured from control (MΦ con ) and SmoKO (MΦ SmoKO ) mice towards mouse gastric organoids. Co-cultures were treated with vehicle or gastrin. B Macrophage migration within the co-cultures was quantified by forward migration index (FMI). * P < 0.001 compared to vehicle, n = 6 individual co-culture experiments. Data are shown as the mean ± SEM.

    Journal: NPJ Regenerative Medicine

    Article Title: Sonic Hedgehog acts as a macrophage chemoattractant during regeneration of the gastric epithelium

    doi: 10.1038/s41536-021-00196-2

    Figure Lengend Snippet: A Immunofluorescence imaging demonstrating migration of CD68+ macrophages cultured from control (MΦ con ) and SmoKO (MΦ SmoKO ) mice towards mouse gastric organoids. Co-cultures were treated with vehicle or gastrin. B Macrophage migration within the co-cultures was quantified by forward migration index (FMI). * P < 0.001 compared to vehicle, n = 6 individual co-culture experiments. Data are shown as the mean ± SEM.

    Article Snippet: The following primary antibodies and dilutions were used: 1:100 mouse anti-CCR2 (Novus Biologicals, NBP1-48338), 1:500 mouse anti-CD68 (Novus Biologicals, NBP2-37265) slides, and whole-mount organoids were imaged on a Zeiss LSM710 LIVE Duo Confocal Microscope.

    Techniques: Immunofluorescence, Imaging, Migration, Cell Culture, Co-Culture Assay

    A Acridine orange assay using gastric organoids treated with either vehicle or histamine. B Shift in F458 (red)/F488 (green) fluorescence ratio was measured in the organoid cultures treated with vehicle or histamine. C Quantitative RT-PCR measuring changes in Shh expression in the gastric organoids treated with vehicle (control) or histamine (Hist). * P < 0.05 compared to control, n = 4 cultures generated from four individual patients. D Bright-field micrographs showing tissue-derived human fundic gastric organoids (huFGOs), PBMC-derived macrophages, and representative co-culture. E Magnetic separation was used to isolate epithelial and immune cells from treated human gastric organoid/macrophage co-cultures. F RT-PCR demonstrating expression of Shh in EpCAM positive and CD68 in macrophage fractions. Data are shown as the mean ± SEM.

    Journal: NPJ Regenerative Medicine

    Article Title: Sonic Hedgehog acts as a macrophage chemoattractant during regeneration of the gastric epithelium

    doi: 10.1038/s41536-021-00196-2

    Figure Lengend Snippet: A Acridine orange assay using gastric organoids treated with either vehicle or histamine. B Shift in F458 (red)/F488 (green) fluorescence ratio was measured in the organoid cultures treated with vehicle or histamine. C Quantitative RT-PCR measuring changes in Shh expression in the gastric organoids treated with vehicle (control) or histamine (Hist). * P < 0.05 compared to control, n = 4 cultures generated from four individual patients. D Bright-field micrographs showing tissue-derived human fundic gastric organoids (huFGOs), PBMC-derived macrophages, and representative co-culture. E Magnetic separation was used to isolate epithelial and immune cells from treated human gastric organoid/macrophage co-cultures. F RT-PCR demonstrating expression of Shh in EpCAM positive and CD68 in macrophage fractions. Data are shown as the mean ± SEM.

    Article Snippet: The following primary antibodies and dilutions were used: 1:100 mouse anti-CCR2 (Novus Biologicals, NBP1-48338), 1:500 mouse anti-CD68 (Novus Biologicals, NBP2-37265) slides, and whole-mount organoids were imaged on a Zeiss LSM710 LIVE Duo Confocal Microscope.

    Techniques: Fluorescence, Quantitative RT-PCR, Expressing, Generated, Derivative Assay, Co-Culture Assay, Reverse Transcription Polymerase Chain Reaction