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    Bio-Rad app app n terminal p
    Summary of Antibodies Used for Immunohistochemistry
    App App N Terminal P, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Summary of Antibodies Used for Immunohistochemistry

    Journal:

    Article Title: Long-Term Accumulation of Amyloid-?, ?-Secretase, Presenilin-1, and Caspase-3 in Damaged Axons Following Brain Trauma

    doi:

    Figure Lengend Snippet: Summary of Antibodies Used for Immunohistochemistry

    Article Snippet: It was coupled covalently through its cysteine residue to maleimide-activated KLH, and the conjugate was used to immunize mice and generate the Ab249 antiserum. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antibody Epitope protein/amino acids Type Dilution Company Reference 22C11 APP/60-100 M 1:40 Chemicon 47 APP APP/C terminal & C99 fragments P 1:200 ProSci 48, 49 APP APP/N terminal P 1:00 Serotec 50 249 caspase cleavage of APP P 1:2000 ** 51 6E10 Aβ/ 1–17 M 1:500 Signet 57 6F/3D Aβ/ 8–17 M 1:100 DAKO 52, 53 10Δ5 Aβ 1–28 M 1:50 * 54–56 4G8 Aβ 17–24 M 1:1000 * 57, 58 Aβ 1–40 Aβ 1–40 P 1:100 Chemicon 59, 60 Aβ 1–40 Aβ 1–40 P 1:1000 Signet 59, 60 70 Aβ 1–40 P 1:1000 ** 61 Aβ 17–42 Aβ 17–42 P 1:100 Chemicon 62–64 Aβ 1–42 Aβ 1–42 P 1:1000 Signet 62–64 13335 Aβ 1–42 P 1:1000 * 14, 65 BCO5 Aβ 1–42(43) M Wako Pure 66, 67 Aβ1 Aβ & C99 fragments P 1:1000 ** 68, 69 N52 NF-H M 1:400 Sigma 70, 71 P20 Procaspase-3 P 1:100 Santa Cruz 72, 73 Large subunit BACE2/ β-secretase P 1:500 Alpha 12, 74 Asp1 PS-1 C-terminal P 1:200 Zymed 75 Presenilin PS-1 N-terminal P 1:50 Chemicon 76 Presenilin L1 kinesin-L M 1:200 Chemicon 77, 78 H1 kinesin-H M 1:200 Chemicon 79 OX42 CD11b M 1:100 Serotec 80 Open in a separate window * , antibodies from Dr. V.M.-Y.

    Techniques:

    Representative photomicrographs demonstrating APP and NF protein accumulation in damaged axons in the subcortical white matter and basal ganglia detected by antibodies to 22C11/APP N-terminal and N52/NF200 at 3, 7 days, and 6 months following injury in the pig. Dark staining represents immunoreactivity of APP and NF proteins in swollen axons. Bar, 25 μm.

    Journal:

    Article Title: Long-Term Accumulation of Amyloid-?, ?-Secretase, Presenilin-1, and Caspase-3 in Damaged Axons Following Brain Trauma

    doi:

    Figure Lengend Snippet: Representative photomicrographs demonstrating APP and NF protein accumulation in damaged axons in the subcortical white matter and basal ganglia detected by antibodies to 22C11/APP N-terminal and N52/NF200 at 3, 7 days, and 6 months following injury in the pig. Dark staining represents immunoreactivity of APP and NF proteins in swollen axons. Bar, 25 μm.

    Article Snippet: It was coupled covalently through its cysteine residue to maleimide-activated KLH, and the conjugate was used to immunize mice and generate the Ab249 antiserum. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antibody Epitope protein/amino acids Type Dilution Company Reference 22C11 APP/60-100 M 1:40 Chemicon 47 APP APP/C terminal & C99 fragments P 1:200 ProSci 48, 49 APP APP/N terminal P 1:00 Serotec 50 249 caspase cleavage of APP P 1:2000 ** 51 6E10 Aβ/ 1–17 M 1:500 Signet 57 6F/3D Aβ/ 8–17 M 1:100 DAKO 52, 53 10Δ5 Aβ 1–28 M 1:50 * 54–56 4G8 Aβ 17–24 M 1:1000 * 57, 58 Aβ 1–40 Aβ 1–40 P 1:100 Chemicon 59, 60 Aβ 1–40 Aβ 1–40 P 1:1000 Signet 59, 60 70 Aβ 1–40 P 1:1000 ** 61 Aβ 17–42 Aβ 17–42 P 1:100 Chemicon 62–64 Aβ 1–42 Aβ 1–42 P 1:1000 Signet 62–64 13335 Aβ 1–42 P 1:1000 * 14, 65 BCO5 Aβ 1–42(43) M Wako Pure 66, 67 Aβ1 Aβ & C99 fragments P 1:1000 ** 68, 69 N52 NF-H M 1:400 Sigma 70, 71 P20 Procaspase-3 P 1:100 Santa Cruz 72, 73 Large subunit BACE2/ β-secretase P 1:500 Alpha 12, 74 Asp1 PS-1 C-terminal P 1:200 Zymed 75 Presenilin PS-1 N-terminal P 1:50 Chemicon 76 Presenilin L1 kinesin-L M 1:200 Chemicon 77, 78 H1 kinesin-H M 1:200 Chemicon 79 OX42 CD11b M 1:100 Serotec 80 Open in a separate window * , antibodies from Dr. V.M.-Y.

    Techniques: Staining