44910 Search Results


anti s100a9  (Novus Biologicals)
93
Novus Biologicals anti s100a9
Reducing sulfur amino acids (SAAs) and increasing peroxidizable polyunsaturated fatty acids (PUFAs) through diet counteracts breast cancer development in mice. ( a ) Percentage of dead TUBO cells after 24 h SAAs depletion with or without 400 μM linoleic acid (LAc). Ferrostatin-1 (FST-1) was used at 5 μM concentration and added 1 h prior treatment. Data are expressed as mean ± S.D. ( n = 4, ** p < 0.01, *** p < 0.001, **** p < 0.0001). ( b , c ) Schematic representation of normal (ND) and low SAA-high PUFA (LSAA/HPUFA) diet composition ( b ) and experimental design of the in vivo study ( c ). ( d ) Body weight was evaluated weekly until to sacrifice (** p < 0.01, * p < 0.05). ( e ) Analysis of bio-clinical parameters after 3 weeks from TUBO cell injection in mice. Dashed lines indicate the range of normal values. Data are expressed as mean ± S.D. ( n = 5 mice ND group; n = 4 mice LSAA/HPUFA group; * p < 0.05 vs. ND). ( f ) Tumor masses were evaluated weekly until to sacrifice. ( g ) Representative photographs of explanted breast cancer masses. ( h , i ) Representative immunofluorescence images of intratumor vessels ( h ) and immune cell infiltrates ( i ) detected by using Von Willebrand and <t>S100A9</t> antibody, respectively. Incubation with AlexaFluor488-conjugated phalloidin and Hoechst 33342 were used to visualize actin cytoskeleton and nuclei, respectively. White arrows in ( i ) indicate the presence of myeloid cells within the tumor mass. Scale bar: 100 μm. ( j , k ) RT-qPCR analysis of the mRNA expression of the inflammation-related genes Il1b, Il6, Tnfa and Nos2 ( j ) and epithelial to mesenchymal transition genes Cdh1, Twist1, Snail1, Snail2 and Vim ( k ). All the images reported are representative of n = 6 mice/group. Data are expressed as mean ± S.D. ( n = 9 mice/group; ** p < 0.01, * p < 0.05 vs. ND).
Anti S100a9, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti s100a9/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
anti s100a9 - by Bioz Stars, 2025-05
93/100 stars
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n27 cells  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology n27 cells
Reducing sulfur amino acids (SAAs) and increasing peroxidizable polyunsaturated fatty acids (PUFAs) through diet counteracts breast cancer development in mice. ( a ) Percentage of dead TUBO cells after 24 h SAAs depletion with or without 400 μM linoleic acid (LAc). Ferrostatin-1 (FST-1) was used at 5 μM concentration and added 1 h prior treatment. Data are expressed as mean ± S.D. ( n = 4, ** p < 0.01, *** p < 0.001, **** p < 0.0001). ( b , c ) Schematic representation of normal (ND) and low SAA-high PUFA (LSAA/HPUFA) diet composition ( b ) and experimental design of the in vivo study ( c ). ( d ) Body weight was evaluated weekly until to sacrifice (** p < 0.01, * p < 0.05). ( e ) Analysis of bio-clinical parameters after 3 weeks from TUBO cell injection in mice. Dashed lines indicate the range of normal values. Data are expressed as mean ± S.D. ( n = 5 mice ND group; n = 4 mice LSAA/HPUFA group; * p < 0.05 vs. ND). ( f ) Tumor masses were evaluated weekly until to sacrifice. ( g ) Representative photographs of explanted breast cancer masses. ( h , i ) Representative immunofluorescence images of intratumor vessels ( h ) and immune cell infiltrates ( i ) detected by using Von Willebrand and <t>S100A9</t> antibody, respectively. Incubation with AlexaFluor488-conjugated phalloidin and Hoechst 33342 were used to visualize actin cytoskeleton and nuclei, respectively. White arrows in ( i ) indicate the presence of myeloid cells within the tumor mass. Scale bar: 100 μm. ( j , k ) RT-qPCR analysis of the mRNA expression of the inflammation-related genes Il1b, Il6, Tnfa and Nos2 ( j ) and epithelial to mesenchymal transition genes Cdh1, Twist1, Snail1, Snail2 and Vim ( k ). All the images reported are representative of n = 6 mice/group. Data are expressed as mean ± S.D. ( n = 9 mice/group; ** p < 0.01, * p < 0.05 vs. ND).
N27 Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n27 cells/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
n27 cells - by Bioz Stars, 2025-05
93/100 stars
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s100a9 monoclonal antibody  (Novus Biologicals)
93
Novus Biologicals s100a9 monoclonal antibody
Reducing sulfur amino acids (SAAs) and increasing peroxidizable polyunsaturated fatty acids (PUFAs) through diet counteracts breast cancer development in mice. ( a ) Percentage of dead TUBO cells after 24 h SAAs depletion with or without 400 μM linoleic acid (LAc). Ferrostatin-1 (FST-1) was used at 5 μM concentration and added 1 h prior treatment. Data are expressed as mean ± S.D. ( n = 4, ** p < 0.01, *** p < 0.001, **** p < 0.0001). ( b , c ) Schematic representation of normal (ND) and low SAA-high PUFA (LSAA/HPUFA) diet composition ( b ) and experimental design of the in vivo study ( c ). ( d ) Body weight was evaluated weekly until to sacrifice (** p < 0.01, * p < 0.05). ( e ) Analysis of bio-clinical parameters after 3 weeks from TUBO cell injection in mice. Dashed lines indicate the range of normal values. Data are expressed as mean ± S.D. ( n = 5 mice ND group; n = 4 mice LSAA/HPUFA group; * p < 0.05 vs. ND). ( f ) Tumor masses were evaluated weekly until to sacrifice. ( g ) Representative photographs of explanted breast cancer masses. ( h , i ) Representative immunofluorescence images of intratumor vessels ( h ) and immune cell infiltrates ( i ) detected by using Von Willebrand and <t>S100A9</t> antibody, respectively. Incubation with AlexaFluor488-conjugated phalloidin and Hoechst 33342 were used to visualize actin cytoskeleton and nuclei, respectively. White arrows in ( i ) indicate the presence of myeloid cells within the tumor mass. Scale bar: 100 μm. ( j , k ) RT-qPCR analysis of the mRNA expression of the inflammation-related genes Il1b, Il6, Tnfa and Nos2 ( j ) and epithelial to mesenchymal transition genes Cdh1, Twist1, Snail1, Snail2 and Vim ( k ). All the images reported are representative of n = 6 mice/group. Data are expressed as mean ± S.D. ( n = 9 mice/group; ** p < 0.01, * p < 0.05 vs. ND).
S100a9 Monoclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s100a9 monoclonal antibody/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
s100a9 monoclonal antibody - by Bioz Stars, 2025-05
93/100 stars
  Buy from Supplier

Image Search Results


Reducing sulfur amino acids (SAAs) and increasing peroxidizable polyunsaturated fatty acids (PUFAs) through diet counteracts breast cancer development in mice. ( a ) Percentage of dead TUBO cells after 24 h SAAs depletion with or without 400 μM linoleic acid (LAc). Ferrostatin-1 (FST-1) was used at 5 μM concentration and added 1 h prior treatment. Data are expressed as mean ± S.D. ( n = 4, ** p < 0.01, *** p < 0.001, **** p < 0.0001). ( b , c ) Schematic representation of normal (ND) and low SAA-high PUFA (LSAA/HPUFA) diet composition ( b ) and experimental design of the in vivo study ( c ). ( d ) Body weight was evaluated weekly until to sacrifice (** p < 0.01, * p < 0.05). ( e ) Analysis of bio-clinical parameters after 3 weeks from TUBO cell injection in mice. Dashed lines indicate the range of normal values. Data are expressed as mean ± S.D. ( n = 5 mice ND group; n = 4 mice LSAA/HPUFA group; * p < 0.05 vs. ND). ( f ) Tumor masses were evaluated weekly until to sacrifice. ( g ) Representative photographs of explanted breast cancer masses. ( h , i ) Representative immunofluorescence images of intratumor vessels ( h ) and immune cell infiltrates ( i ) detected by using Von Willebrand and S100A9 antibody, respectively. Incubation with AlexaFluor488-conjugated phalloidin and Hoechst 33342 were used to visualize actin cytoskeleton and nuclei, respectively. White arrows in ( i ) indicate the presence of myeloid cells within the tumor mass. Scale bar: 100 μm. ( j , k ) RT-qPCR analysis of the mRNA expression of the inflammation-related genes Il1b, Il6, Tnfa and Nos2 ( j ) and epithelial to mesenchymal transition genes Cdh1, Twist1, Snail1, Snail2 and Vim ( k ). All the images reported are representative of n = 6 mice/group. Data are expressed as mean ± S.D. ( n = 9 mice/group; ** p < 0.01, * p < 0.05 vs. ND).

Journal: International Journal of Molecular Sciences

Article Title: Low Sulfur Amino Acid, High Polyunsaturated Fatty Acid Diet Inhibits Breast Cancer Growth

doi: 10.3390/ijms24010249

Figure Lengend Snippet: Reducing sulfur amino acids (SAAs) and increasing peroxidizable polyunsaturated fatty acids (PUFAs) through diet counteracts breast cancer development in mice. ( a ) Percentage of dead TUBO cells after 24 h SAAs depletion with or without 400 μM linoleic acid (LAc). Ferrostatin-1 (FST-1) was used at 5 μM concentration and added 1 h prior treatment. Data are expressed as mean ± S.D. ( n = 4, ** p < 0.01, *** p < 0.001, **** p < 0.0001). ( b , c ) Schematic representation of normal (ND) and low SAA-high PUFA (LSAA/HPUFA) diet composition ( b ) and experimental design of the in vivo study ( c ). ( d ) Body weight was evaluated weekly until to sacrifice (** p < 0.01, * p < 0.05). ( e ) Analysis of bio-clinical parameters after 3 weeks from TUBO cell injection in mice. Dashed lines indicate the range of normal values. Data are expressed as mean ± S.D. ( n = 5 mice ND group; n = 4 mice LSAA/HPUFA group; * p < 0.05 vs. ND). ( f ) Tumor masses were evaluated weekly until to sacrifice. ( g ) Representative photographs of explanted breast cancer masses. ( h , i ) Representative immunofluorescence images of intratumor vessels ( h ) and immune cell infiltrates ( i ) detected by using Von Willebrand and S100A9 antibody, respectively. Incubation with AlexaFluor488-conjugated phalloidin and Hoechst 33342 were used to visualize actin cytoskeleton and nuclei, respectively. White arrows in ( i ) indicate the presence of myeloid cells within the tumor mass. Scale bar: 100 μm. ( j , k ) RT-qPCR analysis of the mRNA expression of the inflammation-related genes Il1b, Il6, Tnfa and Nos2 ( j ) and epithelial to mesenchymal transition genes Cdh1, Twist1, Snail1, Snail2 and Vim ( k ). All the images reported are representative of n = 6 mice/group. Data are expressed as mean ± S.D. ( n = 9 mice/group; ** p < 0.01, * p < 0.05 vs. ND).

Article Snippet: As primary antibodies, we used anti-S100A9 (89726, Novus biologicals, Littleton, CO, USA), anti-Von Willebrand factor (A0082, Dako-Agilent, Glostrup, Denmark) and anti-p(S139)H2AX (20E3, Cell Signaling Technologies) at 1:200 dilution.

Techniques: Concentration Assay, In Vivo, Injection, Immunofluorescence, Incubation, Quantitative RT-PCR, Expressing