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    Bio-Techne corporation anti muc5ac 45m1
    IL-13 induces cell-type-selective gene expression changes in each major subpopulation of bronchial epithelial cells, and enrichment of H3K27ac peaks near IL-13-stimulated genes (A) Scheme for culturing primary human bronchial epithelial cells (HBECs) at an air-liquid interface (ALI) with IL-13 stimulation. On day 0, cells are predominantly basal cells (orange). ALI culture allows differentiation into ciliated (magenta) and secretory cells (cyan). The mucin 5B (MUC5B)-rich mucus gel layer (red) changes to a <t>MUC5AC-rich</t> gel (green) upon IL-13 stimulation. (B and C) Effects of IL-13 on secretory cells compared with basal cells (B) or ciliated cells (C). Each point represents a gene. Genes that were regulated differently between cell types are shown in black (log 2 fold change difference > 1 and FDR < 0.1 for interactions between cell type and cytokine effect), and other genes are shown in gray. R , Pearson correlation coefficient (p < 2.2 × 10 −16 for both comparisons). (D) Relative expression of 20 genes with the highest absolute IL-13-induced fold change in any of the three cell types. (E) Gene set enrichment analysis of IL-13-induced cell type-selective responses in HBECs. Relative enrichment coefficients were calculated for the full collection of 2,555 gene ontology biological process (GOBP) gene sets; results are shown for the five GOBP gene sets that were most highly induced by IL-13 in basal, ciliated, and secretory cells (p < 10 −5 in one cell type and enrichment coefficient < 0.5 in other cell types). (F) IL-13-induced genes in secretory cells are highly enriched for a previously defined set of goblet cell genes not included in GOBP. For comparison, unlabeled points represent values for the full collection of 2,555 GOBP gene sets. (G) Distribution of IL-13-enriched H3K27ac peaks (N = 387) by genomic region classification. (H) Bulk RNA-seq expression changes in genes closest to H3K27ac ChIP-seq peaks that were not significantly affected (p > 0.1) by IL-13 (no Δ, 26,875 peaks) or were significantly (FDR < 0.1) enriched (387 peaks) or depleted (215 peaks) after IL-13 stimulation. Shown are p values for comparison with unaffected peaks by two-sided Wilcoxon test. (I) The most highly enriched motif discovered in regions with enriched H3K27ac after IL-13 stimulation closely resembles a previously defined STAT6 binding motif. See also <xref ref-type=Figure S1 and Tables S1 and Table S7. Single-cell RNA-seq of three major HBEC subsets (basal, ciliated, and secretory) without and with IFN-α, IL-17, or IL-13 stimulation, related to Figure 1 , Table S8. Gene set enrichment analysis of IFN-α, IL-17, or IL-13 response in three major HBEC subsets (basal, ciliated, and secretory), related to Figure 1 , Table S9. Bulk RNA-seq of HBECs without and with IFN-α, IFN-γ, IL-17, IL-13, or IFN-α and IL-13 stimulation, related to Figure 1 . " width="250" height="auto" />
    Anti Muc5ac 45m1, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti muc5ac 45m1/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti muc5ac 45m1 - by Bioz Stars, 2024-06
    93/100 stars
      Buy from Supplier

    90
    ATCC microsporum canis
    IL-13 induces cell-type-selective gene expression changes in each major subpopulation of bronchial epithelial cells, and enrichment of H3K27ac peaks near IL-13-stimulated genes (A) Scheme for culturing primary human bronchial epithelial cells (HBECs) at an air-liquid interface (ALI) with IL-13 stimulation. On day 0, cells are predominantly basal cells (orange). ALI culture allows differentiation into ciliated (magenta) and secretory cells (cyan). The mucin 5B (MUC5B)-rich mucus gel layer (red) changes to a <t>MUC5AC-rich</t> gel (green) upon IL-13 stimulation. (B and C) Effects of IL-13 on secretory cells compared with basal cells (B) or ciliated cells (C). Each point represents a gene. Genes that were regulated differently between cell types are shown in black (log 2 fold change difference > 1 and FDR < 0.1 for interactions between cell type and cytokine effect), and other genes are shown in gray. R , Pearson correlation coefficient (p < 2.2 × 10 −16 for both comparisons). (D) Relative expression of 20 genes with the highest absolute IL-13-induced fold change in any of the three cell types. (E) Gene set enrichment analysis of IL-13-induced cell type-selective responses in HBECs. Relative enrichment coefficients were calculated for the full collection of 2,555 gene ontology biological process (GOBP) gene sets; results are shown for the five GOBP gene sets that were most highly induced by IL-13 in basal, ciliated, and secretory cells (p < 10 −5 in one cell type and enrichment coefficient < 0.5 in other cell types). (F) IL-13-induced genes in secretory cells are highly enriched for a previously defined set of goblet cell genes not included in GOBP. For comparison, unlabeled points represent values for the full collection of 2,555 GOBP gene sets. (G) Distribution of IL-13-enriched H3K27ac peaks (N = 387) by genomic region classification. (H) Bulk RNA-seq expression changes in genes closest to H3K27ac ChIP-seq peaks that were not significantly affected (p > 0.1) by IL-13 (no Δ, 26,875 peaks) or were significantly (FDR < 0.1) enriched (387 peaks) or depleted (215 peaks) after IL-13 stimulation. Shown are p values for comparison with unaffected peaks by two-sided Wilcoxon test. (I) The most highly enriched motif discovered in regions with enriched H3K27ac after IL-13 stimulation closely resembles a previously defined STAT6 binding motif. See also <xref ref-type=Figure S1 and Tables S1 and Table S7. Single-cell RNA-seq of three major HBEC subsets (basal, ciliated, and secretory) without and with IFN-α, IL-17, or IL-13 stimulation, related to Figure 1 , Table S8. Gene set enrichment analysis of IFN-α, IL-17, or IL-13 response in three major HBEC subsets (basal, ciliated, and secretory), related to Figure 1 , Table S9. Bulk RNA-seq of HBECs without and with IFN-α, IFN-γ, IL-17, IL-13, or IFN-α and IL-13 stimulation, related to Figure 1 . " width="250" height="auto" />
    Microsporum Canis, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microsporum canis/product/ATCC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microsporum canis - by Bioz Stars, 2024-06
    90/100 stars
      Buy from Supplier

    N/A
    Standard format Plasmid sent in bacteria as agar stab
      Buy from Supplier

    Image Search Results


    IL-13 induces cell-type-selective gene expression changes in each major subpopulation of bronchial epithelial cells, and enrichment of H3K27ac peaks near IL-13-stimulated genes (A) Scheme for culturing primary human bronchial epithelial cells (HBECs) at an air-liquid interface (ALI) with IL-13 stimulation. On day 0, cells are predominantly basal cells (orange). ALI culture allows differentiation into ciliated (magenta) and secretory cells (cyan). The mucin 5B (MUC5B)-rich mucus gel layer (red) changes to a MUC5AC-rich gel (green) upon IL-13 stimulation. (B and C) Effects of IL-13 on secretory cells compared with basal cells (B) or ciliated cells (C). Each point represents a gene. Genes that were regulated differently between cell types are shown in black (log 2 fold change difference > 1 and FDR < 0.1 for interactions between cell type and cytokine effect), and other genes are shown in gray. R , Pearson correlation coefficient (p < 2.2 × 10 −16 for both comparisons). (D) Relative expression of 20 genes with the highest absolute IL-13-induced fold change in any of the three cell types. (E) Gene set enrichment analysis of IL-13-induced cell type-selective responses in HBECs. Relative enrichment coefficients were calculated for the full collection of 2,555 gene ontology biological process (GOBP) gene sets; results are shown for the five GOBP gene sets that were most highly induced by IL-13 in basal, ciliated, and secretory cells (p < 10 −5 in one cell type and enrichment coefficient < 0.5 in other cell types). (F) IL-13-induced genes in secretory cells are highly enriched for a previously defined set of goblet cell genes not included in GOBP. For comparison, unlabeled points represent values for the full collection of 2,555 GOBP gene sets. (G) Distribution of IL-13-enriched H3K27ac peaks (N = 387) by genomic region classification. (H) Bulk RNA-seq expression changes in genes closest to H3K27ac ChIP-seq peaks that were not significantly affected (p > 0.1) by IL-13 (no Δ, 26,875 peaks) or were significantly (FDR < 0.1) enriched (387 peaks) or depleted (215 peaks) after IL-13 stimulation. Shown are p values for comparison with unaffected peaks by two-sided Wilcoxon test. (I) The most highly enriched motif discovered in regions with enriched H3K27ac after IL-13 stimulation closely resembles a previously defined STAT6 binding motif. See also <xref ref-type=Figure S1 and Tables S1 and Table S7. Single-cell RNA-seq of three major HBEC subsets (basal, ciliated, and secretory) without and with IFN-α, IL-17, or IL-13 stimulation, related to Figure 1 , Table S8. Gene set enrichment analysis of IFN-α, IL-17, or IL-13 response in three major HBEC subsets (basal, ciliated, and secretory), related to Figure 1 , Table S9. Bulk RNA-seq of HBECs without and with IFN-α, IFN-γ, IL-17, IL-13, or IFN-α and IL-13 stimulation, related to Figure 1 . " width="100%" height="100%">

    Journal: Cell Genomics

    Article Title: Genomic characterization and therapeutic utilization of IL-13-responsive sequences in asthma

    doi: 10.1016/j.xgen.2022.100229

    Figure Lengend Snippet: IL-13 induces cell-type-selective gene expression changes in each major subpopulation of bronchial epithelial cells, and enrichment of H3K27ac peaks near IL-13-stimulated genes (A) Scheme for culturing primary human bronchial epithelial cells (HBECs) at an air-liquid interface (ALI) with IL-13 stimulation. On day 0, cells are predominantly basal cells (orange). ALI culture allows differentiation into ciliated (magenta) and secretory cells (cyan). The mucin 5B (MUC5B)-rich mucus gel layer (red) changes to a MUC5AC-rich gel (green) upon IL-13 stimulation. (B and C) Effects of IL-13 on secretory cells compared with basal cells (B) or ciliated cells (C). Each point represents a gene. Genes that were regulated differently between cell types are shown in black (log 2 fold change difference > 1 and FDR < 0.1 for interactions between cell type and cytokine effect), and other genes are shown in gray. R , Pearson correlation coefficient (p < 2.2 × 10 −16 for both comparisons). (D) Relative expression of 20 genes with the highest absolute IL-13-induced fold change in any of the three cell types. (E) Gene set enrichment analysis of IL-13-induced cell type-selective responses in HBECs. Relative enrichment coefficients were calculated for the full collection of 2,555 gene ontology biological process (GOBP) gene sets; results are shown for the five GOBP gene sets that were most highly induced by IL-13 in basal, ciliated, and secretory cells (p < 10 −5 in one cell type and enrichment coefficient < 0.5 in other cell types). (F) IL-13-induced genes in secretory cells are highly enriched for a previously defined set of goblet cell genes not included in GOBP. For comparison, unlabeled points represent values for the full collection of 2,555 GOBP gene sets. (G) Distribution of IL-13-enriched H3K27ac peaks (N = 387) by genomic region classification. (H) Bulk RNA-seq expression changes in genes closest to H3K27ac ChIP-seq peaks that were not significantly affected (p > 0.1) by IL-13 (no Δ, 26,875 peaks) or were significantly (FDR < 0.1) enriched (387 peaks) or depleted (215 peaks) after IL-13 stimulation. Shown are p values for comparison with unaffected peaks by two-sided Wilcoxon test. (I) The most highly enriched motif discovered in regions with enriched H3K27ac after IL-13 stimulation closely resembles a previously defined STAT6 binding motif. See also Figure S1 and Tables S1 and Table S7. Single-cell RNA-seq of three major HBEC subsets (basal, ciliated, and secretory) without and with IFN-α, IL-17, or IL-13 stimulation, related to Figure 1 , Table S8. Gene set enrichment analysis of IFN-α, IL-17, or IL-13 response in three major HBEC subsets (basal, ciliated, and secretory), related to Figure 1 , Table S9. Bulk RNA-seq of HBECs without and with IFN-α, IFN-γ, IL-17, IL-13, or IFN-α and IL-13 stimulation, related to Figure 1 .

    Article Snippet: Anti-MUC5AC (45M1) [DyLight 405] (mouse monoclonal) , Novus Biologicals , Cat# NBP2-32732V.

    Techniques: Expressing, Comparison, RNA Sequencing Assay, ChIP-sequencing, Binding Assay

    CRISPRi targeting of the SPDEF enhancer prevents SPDEF-dependent, IL-13-induced mucus metaplasia and mucostasis (A) Scheme of CRISPRi targeting of SPDEFe . (B–D) CRISPRi effects on gene expression. HBECs were transduced with lentiviruses driving expression of dCas9 - KRAB and non-targeting (NT) control sgRNAs (gray/black) or sgRNAs targeting the SPDEF promoter (red), MUC5AC promoter (blue), or SPDEFe (orange). After differentiation, cells were left unstimulated or stimulated with IL-13 for 7 days, as indicated. Expression of SPDEF (B), FOXA3 (C), and MUC5AC (D) was measured by quantitative real-time PCR. mRNA levels are relative to IL-13-stimulated HBECs with NT control sgRNAs. (E) CRISPRi effects on intracellular MUC5AC were quantified by flow cytometry. Each point corresponds to a different gRNA targeting the indicated region, tested separately in a single culture well from the same donor (B–E). ∗∗p < 0.01, ∗∗∗∗p < 0.0001 for comparison with IL-13-stimulated HBECs with NT control sgRNAs by one-way ANOVA with Dunnett’s post-test (B–E). (F) Effects of targeting SPDEFe and surrounding regions on IL-13-induced MUC5AC production. gRNAs used in (B)–(E) were compared with gRNAs targeting flanking regions ∼2 and 4 kb away from SPDEFe (magenta) in a separate set of experiments (three donors, two replicates per donor). To combine results from two donors, values for MUC5AC-producing cells are shown as percentages of mean values for IL-13-stimulated cells with NT sgRNAs in the same donor. ∗∗p < 0.01, ∗∗∗∗p < 0.0001 compared with IL-13-stimulated HBECs with NT-1 sgRNA by one-way ANOVA with Tukey’s post-test. p values for all comparisons are provided in <xref ref-type=Table S2 . (G–J) CRISPRi effects on mucin staining and mucociliary transport. HBECs were treated as above using NT-2 gRNA, SPDEF -TSS(+34) gRNA, or SPDEFe (+87) gRNA. Sections (G) and extracellular mucus gels from whole-mount preparations (H) were stained for MUC5AC (cyan), MUC5B (red), and the ciliated cell marker ac-α-Tub (yellow). Nuclei were stained with DAPI (purple). Scale bars: 20 μm (G) and 100 μm (H). Images are representative of two experiments with different donors. Mucociliary transport rates were determined from trajectories of fluorescent microspheres placed on gels atop cells (I and J). Shown is superimposition of 10 images acquired at 1-s intervals; scale bars: 50 μm (I). Microsphere speeds were determined from three donors, one well per donor, four fields per well (J). Values represent median microsphere speed for each field. Boxes extend from the 25th to the 75th percentile, horizontal lines within the box indicate means, and whiskers represent minimum and maximum values. ∗p < 0.05, ∗∗p < 0.01 by one-way ANOVA with Tukey’s post-test. See also Figures S7 and and Table S2 . " width="100%" height="100%">

    Journal: Cell Genomics

    Article Title: Genomic characterization and therapeutic utilization of IL-13-responsive sequences in asthma

    doi: 10.1016/j.xgen.2022.100229

    Figure Lengend Snippet: CRISPRi targeting of the SPDEF enhancer prevents SPDEF-dependent, IL-13-induced mucus metaplasia and mucostasis (A) Scheme of CRISPRi targeting of SPDEFe . (B–D) CRISPRi effects on gene expression. HBECs were transduced with lentiviruses driving expression of dCas9 - KRAB and non-targeting (NT) control sgRNAs (gray/black) or sgRNAs targeting the SPDEF promoter (red), MUC5AC promoter (blue), or SPDEFe (orange). After differentiation, cells were left unstimulated or stimulated with IL-13 for 7 days, as indicated. Expression of SPDEF (B), FOXA3 (C), and MUC5AC (D) was measured by quantitative real-time PCR. mRNA levels are relative to IL-13-stimulated HBECs with NT control sgRNAs. (E) CRISPRi effects on intracellular MUC5AC were quantified by flow cytometry. Each point corresponds to a different gRNA targeting the indicated region, tested separately in a single culture well from the same donor (B–E). ∗∗p < 0.01, ∗∗∗∗p < 0.0001 for comparison with IL-13-stimulated HBECs with NT control sgRNAs by one-way ANOVA with Dunnett’s post-test (B–E). (F) Effects of targeting SPDEFe and surrounding regions on IL-13-induced MUC5AC production. gRNAs used in (B)–(E) were compared with gRNAs targeting flanking regions ∼2 and 4 kb away from SPDEFe (magenta) in a separate set of experiments (three donors, two replicates per donor). To combine results from two donors, values for MUC5AC-producing cells are shown as percentages of mean values for IL-13-stimulated cells with NT sgRNAs in the same donor. ∗∗p < 0.01, ∗∗∗∗p < 0.0001 compared with IL-13-stimulated HBECs with NT-1 sgRNA by one-way ANOVA with Tukey’s post-test. p values for all comparisons are provided in Table S2 . (G–J) CRISPRi effects on mucin staining and mucociliary transport. HBECs were treated as above using NT-2 gRNA, SPDEF -TSS(+34) gRNA, or SPDEFe (+87) gRNA. Sections (G) and extracellular mucus gels from whole-mount preparations (H) were stained for MUC5AC (cyan), MUC5B (red), and the ciliated cell marker ac-α-Tub (yellow). Nuclei were stained with DAPI (purple). Scale bars: 20 μm (G) and 100 μm (H). Images are representative of two experiments with different donors. Mucociliary transport rates were determined from trajectories of fluorescent microspheres placed on gels atop cells (I and J). Shown is superimposition of 10 images acquired at 1-s intervals; scale bars: 50 μm (I). Microsphere speeds were determined from three donors, one well per donor, four fields per well (J). Values represent median microsphere speed for each field. Boxes extend from the 25th to the 75th percentile, horizontal lines within the box indicate means, and whiskers represent minimum and maximum values. ∗p < 0.05, ∗∗p < 0.01 by one-way ANOVA with Tukey’s post-test. See also Figures S7 and and Table S2 .

    Article Snippet: Anti-MUC5AC (45M1) [DyLight 405] (mouse monoclonal) , Novus Biologicals , Cat# NBP2-32732V.

    Techniques: Expressing, Transduction, Real-time Polymerase Chain Reaction, Flow Cytometry, Comparison, Staining, Marker

    SPDEFe can be used to drive CRISPRi to prevent IL-13-induced mucin production by secretory cells (A) Scheme of IL-13-responsive, secretory cell-specific, SPDEFe -driven CRISPRi. minP, minimal promoter. (B–H) A lentivirus containing a KRAB - dCas9 transgene driven by SPDEFe (or other enhancers) and a second lentivirus driving expression of sgRNA targeting the SPDEF (SPDEF-TSS(+34)) or MUC5AC (MUC5AC-TSS(+134)) promoter were used in combination. Transduced cells were ALI cultured without cytokine stimulation or with IL-13 (B–E) or IL-1β (F–H) stimulation for the last 7 days of culture, as indicated. Changes in expression of dCas9 (B and F), SPDEF (C and G), and MUC5AC (D and H) were measured by quantitative real-time PCR. MUC5AC-producing cells (E) were quantitated by flow cytometry. mRNA levels and MUC5AC-producing cells are relative to mRNA levels and MUC5AC-producing cells from IL-13-stimulated cells with SV40e - KRAB - dCas9 and NT-2 sgRNA in the same donor. IL-13 data (B–E) are from four donors. The IL-1β data (F–H) are from cells from three donors, including two used for the IL-13 experiment. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001 compared with the unstimulated empty vector control for NT gRNA and IL-13-stimulated empty vector control for SPDEF and MUC5AC gRNAs by one-way ANOVA Tukey’s post-test (B–H).

    Journal: Cell Genomics

    Article Title: Genomic characterization and therapeutic utilization of IL-13-responsive sequences in asthma

    doi: 10.1016/j.xgen.2022.100229

    Figure Lengend Snippet: SPDEFe can be used to drive CRISPRi to prevent IL-13-induced mucin production by secretory cells (A) Scheme of IL-13-responsive, secretory cell-specific, SPDEFe -driven CRISPRi. minP, minimal promoter. (B–H) A lentivirus containing a KRAB - dCas9 transgene driven by SPDEFe (or other enhancers) and a second lentivirus driving expression of sgRNA targeting the SPDEF (SPDEF-TSS(+34)) or MUC5AC (MUC5AC-TSS(+134)) promoter were used in combination. Transduced cells were ALI cultured without cytokine stimulation or with IL-13 (B–E) or IL-1β (F–H) stimulation for the last 7 days of culture, as indicated. Changes in expression of dCas9 (B and F), SPDEF (C and G), and MUC5AC (D and H) were measured by quantitative real-time PCR. MUC5AC-producing cells (E) were quantitated by flow cytometry. mRNA levels and MUC5AC-producing cells are relative to mRNA levels and MUC5AC-producing cells from IL-13-stimulated cells with SV40e - KRAB - dCas9 and NT-2 sgRNA in the same donor. IL-13 data (B–E) are from four donors. The IL-1β data (F–H) are from cells from three donors, including two used for the IL-13 experiment. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001 compared with the unstimulated empty vector control for NT gRNA and IL-13-stimulated empty vector control for SPDEF and MUC5AC gRNAs by one-way ANOVA Tukey’s post-test (B–H).

    Article Snippet: Anti-MUC5AC (45M1) [DyLight 405] (mouse monoclonal) , Novus Biologicals , Cat# NBP2-32732V.

    Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction, Flow Cytometry, Plasmid Preparation

    Journal: Cell Genomics

    Article Title: Genomic characterization and therapeutic utilization of IL-13-responsive sequences in asthma

    doi: 10.1016/j.xgen.2022.100229

    Figure Lengend Snippet:

    Article Snippet: Anti-MUC5AC (45M1) [DyLight 405] (mouse monoclonal) , Novus Biologicals , Cat# NBP2-32732V.

    Techniques: Recombinant, SYBR Green Assay, Software