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  • 96
    Bio-Techne corporation dylight 488 rat monoclonal anti mouse cd31 pecam 1 antibody
    OP treatment of RAGxCγ double mutant mice bearing heterotopic xenograft of MDA-MB-231 tumors. Notes: Cells at 0.5×10 6 in 0.2 mL were implanted cutaneously in the right back flank of mice. Twice a week following implantation of the cancer cells, each mouse was monitored for tumor volume ([width squared/2] × length) at the site of implantation. Mice were treated with 30 mg/kg and 50 mg/kg of OP in sterile saline ip daily at day 10 postimplantation, when the tumor volume reached ~10–20 mm 3 . ( A ) Necropsy tumors, H&E staining of tumors, paraffin-embedded tumor sections (5 μm) on glass slides were processed for immunohistochemistry using primary <t>DyLight</t> <t>488-conjugated</t> rat monoclonal anti-mouse <t>CD31/PECAM-1</t> antibody, primary anti-E-cadherin, and N-cadherin antibodies, followed with polyclonal goat anti-rabbit Alexa Fluor ® 488 secondary antibody in 1% BSA in PBS blocking solution and Entellan ® rapid mounting medium. Background control (2nd Ab ctrl) sections were prepared without the primary antibodies. Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescence camera attached to a Zeiss Imager M2 fluorescence microscope at 200× magnification. Images are representative of at least five fields of view from two tumor sections. ( B ) Quantitative analysis was done by assessing the density of tumor staining corrected for background in each panel using Corel Photo Paint 8.0 software. Each bar in the figure represents the mean (± SEM) corrected density of tumor staining within the respective images; ( C ) Enlarged visualized image of live necropsy tumors; ( D ) Tumor growth rates for individual mice (mouse label A1, A2, and A4 for the control cohort; B1–4, 30 mg/kg OP cohort; and C1 and C3, 50 mg/kg cohort); ( E ) Mean ± SEM of live necropsy tumor weight per mouse body weight; Statistical analysis was carried out using GraphPad Prism, and results were compared by unpaired t -test. Abbreviations: BSA, bovine serum albumin; H&E, hematoxylin and eosin; ip, intraperitoneally; ns, not significant; OP, oseltamivir phosphate; PBS, phosphate-buffered saline; SEM, standard error of the mean.
    Dylight 488 Rat Monoclonal Anti Mouse Cd31 Pecam 1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight 488 rat monoclonal anti mouse cd31 pecam 1 antibody/product/Bio-Techne corporation
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    90
    ATCC mole cular cell biology
    OP treatment of RAGxCγ double mutant mice bearing heterotopic xenograft of MDA-MB-231 tumors. Notes: Cells at 0.5×10 6 in 0.2 mL were implanted cutaneously in the right back flank of mice. Twice a week following implantation of the cancer cells, each mouse was monitored for tumor volume ([width squared/2] × length) at the site of implantation. Mice were treated with 30 mg/kg and 50 mg/kg of OP in sterile saline ip daily at day 10 postimplantation, when the tumor volume reached ~10–20 mm 3 . ( A ) Necropsy tumors, H&E staining of tumors, paraffin-embedded tumor sections (5 μm) on glass slides were processed for immunohistochemistry using primary <t>DyLight</t> <t>488-conjugated</t> rat monoclonal anti-mouse <t>CD31/PECAM-1</t> antibody, primary anti-E-cadherin, and N-cadherin antibodies, followed with polyclonal goat anti-rabbit Alexa Fluor ® 488 secondary antibody in 1% BSA in PBS blocking solution and Entellan ® rapid mounting medium. Background control (2nd Ab ctrl) sections were prepared without the primary antibodies. Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescence camera attached to a Zeiss Imager M2 fluorescence microscope at 200× magnification. Images are representative of at least five fields of view from two tumor sections. ( B ) Quantitative analysis was done by assessing the density of tumor staining corrected for background in each panel using Corel Photo Paint 8.0 software. Each bar in the figure represents the mean (± SEM) corrected density of tumor staining within the respective images; ( C ) Enlarged visualized image of live necropsy tumors; ( D ) Tumor growth rates for individual mice (mouse label A1, A2, and A4 for the control cohort; B1–4, 30 mg/kg OP cohort; and C1 and C3, 50 mg/kg cohort); ( E ) Mean ± SEM of live necropsy tumor weight per mouse body weight; Statistical analysis was carried out using GraphPad Prism, and results were compared by unpaired t -test. Abbreviations: BSA, bovine serum albumin; H&E, hematoxylin and eosin; ip, intraperitoneally; ns, not significant; OP, oseltamivir phosphate; PBS, phosphate-buffered saline; SEM, standard error of the mean.
    Mole Cular Cell Biology, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mole cular cell biology/product/ATCC
    Average 90 stars, based on 1 article reviews
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    mole cular cell biology - by Bioz Stars, 2024-06
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    92
    DSMZ thermobifida fusca yx
    OP treatment of RAGxCγ double mutant mice bearing heterotopic xenograft of MDA-MB-231 tumors. Notes: Cells at 0.5×10 6 in 0.2 mL were implanted cutaneously in the right back flank of mice. Twice a week following implantation of the cancer cells, each mouse was monitored for tumor volume ([width squared/2] × length) at the site of implantation. Mice were treated with 30 mg/kg and 50 mg/kg of OP in sterile saline ip daily at day 10 postimplantation, when the tumor volume reached ~10–20 mm 3 . ( A ) Necropsy tumors, H&E staining of tumors, paraffin-embedded tumor sections (5 μm) on glass slides were processed for immunohistochemistry using primary <t>DyLight</t> <t>488-conjugated</t> rat monoclonal anti-mouse <t>CD31/PECAM-1</t> antibody, primary anti-E-cadherin, and N-cadherin antibodies, followed with polyclonal goat anti-rabbit Alexa Fluor ® 488 secondary antibody in 1% BSA in PBS blocking solution and Entellan ® rapid mounting medium. Background control (2nd Ab ctrl) sections were prepared without the primary antibodies. Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescence camera attached to a Zeiss Imager M2 fluorescence microscope at 200× magnification. Images are representative of at least five fields of view from two tumor sections. ( B ) Quantitative analysis was done by assessing the density of tumor staining corrected for background in each panel using Corel Photo Paint 8.0 software. Each bar in the figure represents the mean (± SEM) corrected density of tumor staining within the respective images; ( C ) Enlarged visualized image of live necropsy tumors; ( D ) Tumor growth rates for individual mice (mouse label A1, A2, and A4 for the control cohort; B1–4, 30 mg/kg OP cohort; and C1 and C3, 50 mg/kg cohort); ( E ) Mean ± SEM of live necropsy tumor weight per mouse body weight; Statistical analysis was carried out using GraphPad Prism, and results were compared by unpaired t -test. Abbreviations: BSA, bovine serum albumin; H&E, hematoxylin and eosin; ip, intraperitoneally; ns, not significant; OP, oseltamivir phosphate; PBS, phosphate-buffered saline; SEM, standard error of the mean.
    Thermobifida Fusca Yx, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore 44336 44341 salt
    OP treatment of RAGxCγ double mutant mice bearing heterotopic xenograft of MDA-MB-231 tumors. Notes: Cells at 0.5×10 6 in 0.2 mL were implanted cutaneously in the right back flank of mice. Twice a week following implantation of the cancer cells, each mouse was monitored for tumor volume ([width squared/2] × length) at the site of implantation. Mice were treated with 30 mg/kg and 50 mg/kg of OP in sterile saline ip daily at day 10 postimplantation, when the tumor volume reached ~10–20 mm 3 . ( A ) Necropsy tumors, H&E staining of tumors, paraffin-embedded tumor sections (5 μm) on glass slides were processed for immunohistochemistry using primary <t>DyLight</t> <t>488-conjugated</t> rat monoclonal anti-mouse <t>CD31/PECAM-1</t> antibody, primary anti-E-cadherin, and N-cadherin antibodies, followed with polyclonal goat anti-rabbit Alexa Fluor ® 488 secondary antibody in 1% BSA in PBS blocking solution and Entellan ® rapid mounting medium. Background control (2nd Ab ctrl) sections were prepared without the primary antibodies. Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescence camera attached to a Zeiss Imager M2 fluorescence microscope at 200× magnification. Images are representative of at least five fields of view from two tumor sections. ( B ) Quantitative analysis was done by assessing the density of tumor staining corrected for background in each panel using Corel Photo Paint 8.0 software. Each bar in the figure represents the mean (± SEM) corrected density of tumor staining within the respective images; ( C ) Enlarged visualized image of live necropsy tumors; ( D ) Tumor growth rates for individual mice (mouse label A1, A2, and A4 for the control cohort; B1–4, 30 mg/kg OP cohort; and C1 and C3, 50 mg/kg cohort); ( E ) Mean ± SEM of live necropsy tumor weight per mouse body weight; Statistical analysis was carried out using GraphPad Prism, and results were compared by unpaired t -test. Abbreviations: BSA, bovine serum albumin; H&E, hematoxylin and eosin; ip, intraperitoneally; ns, not significant; OP, oseltamivir phosphate; PBS, phosphate-buffered saline; SEM, standard error of the mean.
    44336 44341 Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/44336 44341 salt/product/Millipore
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    86
    Celestron International 44341 photo 10 1 situation
    OP treatment of RAGxCγ double mutant mice bearing heterotopic xenograft of MDA-MB-231 tumors. Notes: Cells at 0.5×10 6 in 0.2 mL were implanted cutaneously in the right back flank of mice. Twice a week following implantation of the cancer cells, each mouse was monitored for tumor volume ([width squared/2] × length) at the site of implantation. Mice were treated with 30 mg/kg and 50 mg/kg of OP in sterile saline ip daily at day 10 postimplantation, when the tumor volume reached ~10–20 mm 3 . ( A ) Necropsy tumors, H&E staining of tumors, paraffin-embedded tumor sections (5 μm) on glass slides were processed for immunohistochemistry using primary <t>DyLight</t> <t>488-conjugated</t> rat monoclonal anti-mouse <t>CD31/PECAM-1</t> antibody, primary anti-E-cadherin, and N-cadherin antibodies, followed with polyclonal goat anti-rabbit Alexa Fluor ® 488 secondary antibody in 1% BSA in PBS blocking solution and Entellan ® rapid mounting medium. Background control (2nd Ab ctrl) sections were prepared without the primary antibodies. Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescence camera attached to a Zeiss Imager M2 fluorescence microscope at 200× magnification. Images are representative of at least five fields of view from two tumor sections. ( B ) Quantitative analysis was done by assessing the density of tumor staining corrected for background in each panel using Corel Photo Paint 8.0 software. Each bar in the figure represents the mean (± SEM) corrected density of tumor staining within the respective images; ( C ) Enlarged visualized image of live necropsy tumors; ( D ) Tumor growth rates for individual mice (mouse label A1, A2, and A4 for the control cohort; B1–4, 30 mg/kg OP cohort; and C1 and C3, 50 mg/kg cohort); ( E ) Mean ± SEM of live necropsy tumor weight per mouse body weight; Statistical analysis was carried out using GraphPad Prism, and results were compared by unpaired t -test. Abbreviations: BSA, bovine serum albumin; H&E, hematoxylin and eosin; ip, intraperitoneally; ns, not significant; OP, oseltamivir phosphate; PBS, phosphate-buffered saline; SEM, standard error of the mean.
    44341 Photo 10 1 Situation, supplied by Celestron International, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    OP treatment of RAGxCγ double mutant mice bearing heterotopic xenograft of MDA-MB-231 tumors. Notes: Cells at 0.5×10 6 in 0.2 mL were implanted cutaneously in the right back flank of mice. Twice a week following implantation of the cancer cells, each mouse was monitored for tumor volume ([width squared/2] × length) at the site of implantation. Mice were treated with 30 mg/kg and 50 mg/kg of OP in sterile saline ip daily at day 10 postimplantation, when the tumor volume reached ~10–20 mm 3 . ( A ) Necropsy tumors, H&E staining of tumors, paraffin-embedded tumor sections (5 μm) on glass slides were processed for immunohistochemistry using primary DyLight 488-conjugated rat monoclonal anti-mouse CD31/PECAM-1 antibody, primary anti-E-cadherin, and N-cadherin antibodies, followed with polyclonal goat anti-rabbit Alexa Fluor ® 488 secondary antibody in 1% BSA in PBS blocking solution and Entellan ® rapid mounting medium. Background control (2nd Ab ctrl) sections were prepared without the primary antibodies. Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescence camera attached to a Zeiss Imager M2 fluorescence microscope at 200× magnification. Images are representative of at least five fields of view from two tumor sections. ( B ) Quantitative analysis was done by assessing the density of tumor staining corrected for background in each panel using Corel Photo Paint 8.0 software. Each bar in the figure represents the mean (± SEM) corrected density of tumor staining within the respective images; ( C ) Enlarged visualized image of live necropsy tumors; ( D ) Tumor growth rates for individual mice (mouse label A1, A2, and A4 for the control cohort; B1–4, 30 mg/kg OP cohort; and C1 and C3, 50 mg/kg cohort); ( E ) Mean ± SEM of live necropsy tumor weight per mouse body weight; Statistical analysis was carried out using GraphPad Prism, and results were compared by unpaired t -test. Abbreviations: BSA, bovine serum albumin; H&E, hematoxylin and eosin; ip, intraperitoneally; ns, not significant; OP, oseltamivir phosphate; PBS, phosphate-buffered saline; SEM, standard error of the mean.

    Journal: Breast Cancer : Targets and Therapy

    Article Title: Oseltamivir phosphate monotherapy ablates tumor neovascularization, growth, and metastasis in mouse model of human triple-negative breast adenocarcinoma

    doi: 10.2147/BCTT.S74663

    Figure Lengend Snippet: OP treatment of RAGxCγ double mutant mice bearing heterotopic xenograft of MDA-MB-231 tumors. Notes: Cells at 0.5×10 6 in 0.2 mL were implanted cutaneously in the right back flank of mice. Twice a week following implantation of the cancer cells, each mouse was monitored for tumor volume ([width squared/2] × length) at the site of implantation. Mice were treated with 30 mg/kg and 50 mg/kg of OP in sterile saline ip daily at day 10 postimplantation, when the tumor volume reached ~10–20 mm 3 . ( A ) Necropsy tumors, H&E staining of tumors, paraffin-embedded tumor sections (5 μm) on glass slides were processed for immunohistochemistry using primary DyLight 488-conjugated rat monoclonal anti-mouse CD31/PECAM-1 antibody, primary anti-E-cadherin, and N-cadherin antibodies, followed with polyclonal goat anti-rabbit Alexa Fluor ® 488 secondary antibody in 1% BSA in PBS blocking solution and Entellan ® rapid mounting medium. Background control (2nd Ab ctrl) sections were prepared without the primary antibodies. Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescence camera attached to a Zeiss Imager M2 fluorescence microscope at 200× magnification. Images are representative of at least five fields of view from two tumor sections. ( B ) Quantitative analysis was done by assessing the density of tumor staining corrected for background in each panel using Corel Photo Paint 8.0 software. Each bar in the figure represents the mean (± SEM) corrected density of tumor staining within the respective images; ( C ) Enlarged visualized image of live necropsy tumors; ( D ) Tumor growth rates for individual mice (mouse label A1, A2, and A4 for the control cohort; B1–4, 30 mg/kg OP cohort; and C1 and C3, 50 mg/kg cohort); ( E ) Mean ± SEM of live necropsy tumor weight per mouse body weight; Statistical analysis was carried out using GraphPad Prism, and results were compared by unpaired t -test. Abbreviations: BSA, bovine serum albumin; H&E, hematoxylin and eosin; ip, intraperitoneally; ns, not significant; OP, oseltamivir phosphate; PBS, phosphate-buffered saline; SEM, standard error of the mean.

    Article Snippet: DyLight™ 488 rat monoclonal anti-mouse CD31/PECAM-1 antibody (Novus Biologicals Canada ULC, Oakville, ON, Canada) was used at a 1:300 dilution according to the manufacturer’s instructions to detect mouse CD31+/PECAM-1 endothelial cells in the archived paraffin-embedded xenogafts of MDA-MB-231 tumors.

    Techniques: Mutagenesis, Sterility, Saline, Staining, Immunohistochemistry, Blocking Assay, Fluorescence, Microscopy, Software