Journal: Nature Communications

Article Title: Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy

doi: 10.1038/s41467-019-12282-7

Figure Lengend Snippet: Downstream targets of Src in mouse and cellular models of SBMA. a – c Immunoblots showing the levels of phosphorylated and total p130Cas, Stat3, p38MAPK, and Akt proteins in the spinal cord and skeletal muscle of 9-week-old AR-97Q mice treated with or without A419259 ( a ). Quantitative analyses were performed using densitometry ( b , c ). d – f Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, p38MAPK, and Akt proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q with or without 0.2 µM of A419259 administration ( d ). Quantitative analyses were performed using densitometry ( e , f ). g – i Immunoblots showing the levels of the phosphorylated and total Src, p130Cas, Stat3, and p38MAPK proteins in the NSC34 and C2C12 cells transfected with mock or Src plasmids ( g ). Quantitative analyses were performed using densitometry ( h , i ). Quantitative analyses were performed on samples from n = 3 animals per group. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f , h , i ). * p < 0.05 and ** p < 0.01. N.S., not significant. Source data are provided as a Source Data file ( a – i )

Article Snippet: Another siRNA to knockdown p130Cas (p130Cas siRNA-2) were p130Cas siRNA (sc-36142) (Santa Cruz Biotechnology, Texas, USA).

Techniques: Western Blot, Stable Transfection, Expressing, Transfection

Journal: Nature Communications

Article Title: Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy

doi: 10.1038/s41467-019-12282-7

Figure Lengend Snippet: p130Cas is activated in cellular and mouse models of SBMA. a , b Immunoblots showing the levels of the phosphorylated and total p130Cas proteins in NSC34 cells and C2C12 cells stably expressing AR-24Q or AR-97Q that were treated with or without DHT ( a ). Quantitative analyses were performed using densitometry ( n = 3) ( b ). c – e Immunoblots showing levels of the p-p130Cas and p130Cas proteins in the spinal cord and skeletal muscle of wild-type and AR-97Q mice at 6, 9 and 13 weeks of age ( c ). Quantitative analyses were performed using densitometry ( n = 3) ( d , e ). f , g Immunohistochemistry for p-p130Cas in spinal cord ( f ) and skeletal muscle ( g ) samples from 6-week-old mice. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , d , e ). * p < 0.05 and ** p < 0.01. Wt, wild-type; AR, AR-97Q; N.S., not significant. Scale bars: 25 µm ( f, g ). Source data are provided as a Source Data file ( a – e )

Article Snippet: Another siRNA to knockdown p130Cas (p130Cas siRNA-2) were p130Cas siRNA (sc-36142) (Santa Cruz Biotechnology, Texas, USA).

Techniques: Western Blot, Stable Transfection, Expressing, Immunohistochemistry

Journal: Nature Communications

Article Title: Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy

doi: 10.1038/s41467-019-12282-7

Figure Lengend Snippet: Phosphorylation of p130Cas exerts toxic effects on cellular models of SBMA. a Immunoblots showing the levels of the p-p130Cas and p130Cas proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q and transfected with the mock or p130Cas siRNA-1. b , c The viability, cell numbers and LDH release from NSC34 ( b ) and C2C12 cells ( c ) are shown ( n = 3). d Immunoblots showing the levels of phosphorylated and total p130Cas proteins in DHT-treated NSC34 and C2C12 cells stably expressing AR-97Q and transfected with the mock or p130Cas vector. e , f Viability, cell numbers and LDH release from NSC34 ( e ) and C2C12 ( f ) cells are shown ( n = 3). Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , c , e , f ). * p < 0.05 and ** p < 0.01. Source data are provided as a Source Data file ( a – f )

Article Snippet: Another siRNA to knockdown p130Cas (p130Cas siRNA-2) were p130Cas siRNA (sc-36142) (Santa Cruz Biotechnology, Texas, USA).

Techniques: Western Blot, Stable Transfection, Expressing, Transfection, Plasmid Preparation

Journal: Acta Stomatologica Croatica

Article Title: A Comparison of the Color Stability of Conventional and CAD/CAM Polymethyl Methacrylate Denture Base Materials

doi: 10.15644/asc53/2/8

Figure Lengend Snippet: Materials and manufacturer information

Article Snippet: CAD/CAM block resin , C , M-PM Disc 44317 , Merz Dental GmbH, Lütjenburg, Gemany.

Techniques: Blocking Assay

Journal: Human Vaccines & Immunotherapeutics

Article Title: A scoping review of active, participant centred, digital adverse events following immunization (AEFI) surveillance of WHO approved COVID-19 vaccines: A Canadian immunization Research Network study

doi: 10.1080/21645515.2023.2293550

Figure Lengend Snippet: Study characteristics.

Article Snippet: Presby et al. – USA , Individuals wearing WHOOP device Boston MA-44317 , Oxford-AstraZeneca, Johnson, Pfizer-BioNTech, Moderna , Biometric device , Cross-sectional-Survey , Wearable biometric device , 1 week before and after.

Techniques: Vaccines, Software, Northern Blot, Injection