44269 Search Results


93
Santa Cruz Biotechnology jnk1 sirna
Pin1 strikingly reverses <t>JNK1‐repressed</t> activity of TAp63γ and makes it hyperactive. (A) Saos‐2 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (B) Saos‐2 cells transfected with indicated plasmids were subjected to cell survival measurement with MTT. Cell viabilities were presented as mentioned above ( n = 3). Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (C) Saos‐2 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids or siRNAs. Bax‐Luc expression levels were measured and presented as mentioned above, while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01; NS, nonsignificant. The error bars (A–C) indicate SD ( n = 3).
Jnk1 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jnk1 sirna/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
jnk1 sirna - by Bioz Stars, 2025-06
93/100 stars
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91
Santa Cruz Biotechnology jnk1 shrna
Pin1 strikingly reverses <t>JNK1‐repressed</t> activity of TAp63γ and makes it hyperactive. (A) Saos‐2 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (B) Saos‐2 cells transfected with indicated plasmids were subjected to cell survival measurement with MTT. Cell viabilities were presented as mentioned above ( n = 3). Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (C) Saos‐2 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids or siRNAs. Bax‐Luc expression levels were measured and presented as mentioned above, while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01; NS, nonsignificant. The error bars (A–C) indicate SD ( n = 3).
Jnk1 Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jnk1 shrna/product/Santa Cruz Biotechnology
Average 91 stars, based on 1 article reviews
jnk1 shrna - by Bioz Stars, 2025-06
91/100 stars
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93
DSMZ dsm 44272 t
Maximum likelihood phylogenetic tree inferred from 16S rRNA gene sequences, showing the phylogenetic position of the strains DSM 44205 T , DSM 44268 T , DSM 44269, DSM 44270 T and <t>DSM</t> <t>44272</t> <t>T</t> relative to the type strains within Geodermatophilaceae . The branches are scaled in terms of the expected number of substitutions per site (see size bar). Support values from maximum-likelihood constrained bootstrapping are shown above the branches if equal to or larger than 60%.
Dsm 44272 T, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
dsm 44272 t - by Bioz Stars, 2025-06
93/100 stars
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Standard format Plasmid sent in bacteria as agar stab
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Image Search Results


Pin1 strikingly reverses JNK1‐repressed activity of TAp63γ and makes it hyperactive. (A) Saos‐2 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (B) Saos‐2 cells transfected with indicated plasmids were subjected to cell survival measurement with MTT. Cell viabilities were presented as mentioned above ( n = 3). Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (C) Saos‐2 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids or siRNAs. Bax‐Luc expression levels were measured and presented as mentioned above, while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01; NS, nonsignificant. The error bars (A–C) indicate SD ( n = 3).

Journal: FEBS Open Bio

Article Title: Pin1 and JNK1 cooperatively modulate TAp63γ

doi: 10.1002/2211-5463.13109

Figure Lengend Snippet: Pin1 strikingly reverses JNK1‐repressed activity of TAp63γ and makes it hyperactive. (A) Saos‐2 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (B) Saos‐2 cells transfected with indicated plasmids were subjected to cell survival measurement with MTT. Cell viabilities were presented as mentioned above ( n = 3). Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (C) Saos‐2 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids or siRNAs. Bax‐Luc expression levels were measured and presented as mentioned above, while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01; NS, nonsignificant. The error bars (A–C) indicate SD ( n = 3).

Article Snippet: JNK1 siRNA and scrambled control were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activity Assay, Transfection, Expressing, Two Tailed Test

JNK1 may repress or promote transactivity of TAp63γ depending on Pin1 level. (A) Saos‐2, Hela and H1299 cells were lysed and indicated proteins were detected by means of IB analysis. (B) Hela cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (C, D) Hela cells, or Hela cells stably ablated with Pin1, were transfected with a mixture of Bax‐Luc and TK‐Renilla, plus HA‐TAp63γ and increasing amounts of JNK1 plasmid as indicated. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. (E) H1299 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. The error bars (B–E) indicate SD.

Journal: FEBS Open Bio

Article Title: Pin1 and JNK1 cooperatively modulate TAp63γ

doi: 10.1002/2211-5463.13109

Figure Lengend Snippet: JNK1 may repress or promote transactivity of TAp63γ depending on Pin1 level. (A) Saos‐2, Hela and H1299 cells were lysed and indicated proteins were detected by means of IB analysis. (B) Hela cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. (C, D) Hela cells, or Hela cells stably ablated with Pin1, were transfected with a mixture of Bax‐Luc and TK‐Renilla, plus HA‐TAp63γ and increasing amounts of JNK1 plasmid as indicated. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. (E) H1299 cells were transfected with a mixture of Bax‐Luc and TK‐Renilla plus indicated plasmids. Bax‐Luc expression levels were measured and presented as mentioned above ( n = 3), while IB analyses were performed to detect indicated proteins. Two‐tailed t‐ test was used for comparison between two groups; ** P < 0.01. The error bars (B–E) indicate SD.

Article Snippet: JNK1 siRNA and scrambled control were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Transfection, Expressing, Two Tailed Test, Stable Transfection, Plasmid Preparation

Maximum likelihood phylogenetic tree inferred from 16S rRNA gene sequences, showing the phylogenetic position of the strains DSM 44205 T , DSM 44268 T , DSM 44269, DSM 44270 T and DSM 44272 T relative to the type strains within Geodermatophilaceae . The branches are scaled in terms of the expected number of substitutions per site (see size bar). Support values from maximum-likelihood constrained bootstrapping are shown above the branches if equal to or larger than 60%.

Journal: Frontiers in Microbiology

Article Title: Genotype–phenotype correlations within the Geodermatophilaceae

doi: 10.3389/fmicb.2022.975365

Figure Lengend Snippet: Maximum likelihood phylogenetic tree inferred from 16S rRNA gene sequences, showing the phylogenetic position of the strains DSM 44205 T , DSM 44268 T , DSM 44269, DSM 44270 T and DSM 44272 T relative to the type strains within Geodermatophilaceae . The branches are scaled in terms of the expected number of substitutions per site (see size bar). Support values from maximum-likelihood constrained bootstrapping are shown above the branches if equal to or larger than 60%.

Article Snippet: All strains were subsequently accessed in the DSMZ open collection as DSM 44268 T , DSM 44269, DSM 44270 T , DSM 44272 T , and DSM 44205 T , respectively.

Techniques:

Phenotypic characteristics of strains DSM 44205 T , DSM 44268 T , DSM 44269, DSM 44270 T and  DSM 44272 T  in comparison with the validly named species in the genus Blastococcus.

Journal: Frontiers in Microbiology

Article Title: Genotype–phenotype correlations within the Geodermatophilaceae

doi: 10.3389/fmicb.2022.975365

Figure Lengend Snippet: Phenotypic characteristics of strains DSM 44205 T , DSM 44268 T , DSM 44269, DSM 44270 T and DSM 44272 T in comparison with the validly named species in the genus Blastococcus.

Article Snippet: All strains were subsequently accessed in the DSMZ open collection as DSM 44268 T , DSM 44269, DSM 44270 T , DSM 44272 T , and DSM 44205 T , respectively.

Techniques:

Polar lipids profile of strains DSM 44205 T (A) , DSM 44268 T (B) , DSM 44269 (C) , DSM 44270 T (D) and DSM 44272 T (E) after separation by two-dimensional TLC using the solvents chloroform:methanol:water (65:25:4; v:v:v) in the first dimension and chloroform:methanol:acetic acid:water (80:12:15:4; v:v:v:v) in the second one. Plates were sprayed with molybdatophosphoric acid (3.5%; Merck™) for detection of the total polar lipids. DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PC, phosphatidylcholine; GPI, glycophosphatidylinositol; PL1-3, Phospholipid; GL1-4, glycolipid; AL1-2, aminolipid; L1-10, unidentified lipids. All data are from this study.

Journal: Frontiers in Microbiology

Article Title: Genotype–phenotype correlations within the Geodermatophilaceae

doi: 10.3389/fmicb.2022.975365

Figure Lengend Snippet: Polar lipids profile of strains DSM 44205 T (A) , DSM 44268 T (B) , DSM 44269 (C) , DSM 44270 T (D) and DSM 44272 T (E) after separation by two-dimensional TLC using the solvents chloroform:methanol:water (65:25:4; v:v:v) in the first dimension and chloroform:methanol:acetic acid:water (80:12:15:4; v:v:v:v) in the second one. Plates were sprayed with molybdatophosphoric acid (3.5%; Merck™) for detection of the total polar lipids. DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PC, phosphatidylcholine; GPI, glycophosphatidylinositol; PL1-3, Phospholipid; GL1-4, glycolipid; AL1-2, aminolipid; L1-10, unidentified lipids. All data are from this study.

Article Snippet: All strains were subsequently accessed in the DSMZ open collection as DSM 44268 T , DSM 44269, DSM 44270 T , DSM 44272 T , and DSM 44205 T , respectively.

Techniques: