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  • 91
    ATCC dsm 44261
    Dsm 44261, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsm 44261/product/ATCC
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dsm 44261 - by Bioz Stars, 2024-04
    91/100 stars
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    93
    Santa Cruz Biotechnology grp78 sirna
    Dox inhibited DT-010-induced <t>GRP78</t> expression in MCF-7 cells. Immunoblots (A) and densitometric analysis (C) of the expression of GRP78 protein were determined after DT-010 treatment in MCF-7 cells. (B) MCF-7 cells were treated with DT-010 for 24 h in the presence or absence of Dox. Representative immunoblots for the expression of GRP78 and β-actin proteins were exhibited. (D) Densitometric analysis showed the expression of GRP78 protein. # P < 0.05 vs. Ctrl.
    Grp78 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grp78 sirna/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    grp78 sirna - by Bioz Stars, 2024-04
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    85
    Santa Cruz Biotechnology grp78 shrna h2 lentivirus
    Dox inhibited DT-010-induced <t>GRP78</t> expression in MCF-7 cells. Immunoblots (A) and densitometric analysis (C) of the expression of GRP78 protein were determined after DT-010 treatment in MCF-7 cells. (B) MCF-7 cells were treated with DT-010 for 24 h in the presence or absence of Dox. Representative immunoblots for the expression of GRP78 and β-actin proteins were exhibited. (D) Densitometric analysis showed the expression of GRP78 protein. # P < 0.05 vs. Ctrl.
    Grp78 Shrna H2 Lentivirus, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grp78 shrna h2 lentivirus/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    grp78 shrna h2 lentivirus - by Bioz Stars, 2024-04
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    85
    Santa Cruz Biotechnology grp78 shrna lentiviral particles
    ( A ) U-2 OS and MG-63 cells were treated with conventional chemotherapeutic drugs in the concentration of their IC 50 values for OS cells for 24 hr and detected by qRT-PCR. The Δ cycle threshold method was used for the calculation of relative differences in mRNA abundance. Data were normalized to the expression of GAPDH . The results of real-time RT-PCR were expressed as fold-changes. The normalized value of the target mRNA of the control group is arbitrarily presented as 1 ( n = 6). ( B ) U-2 OS and MG-63 cells were treated with 1 μM DOX for 0 to 72 hr. The expression of P-gp at the membrane surface and the levels of <t>GRP78,</t> p-Akt and Akt in the whole-cell lysates were detected by Western blot and normalized by GAPDH ( n = 3). ( C ) Nude mice bearing xenograft tumors were treated with DOX by intraperitoneal injection once every four days. Three mice were selected randomly and sacrificed every day. Xenografts were removed, fixed and paraffin embedded. IHC staining was performed by using P-gp, GRP78 and p-Akt antibodies (×400) ( n = 6). ( D ) Western blot results of the membrane lysates or total lysates from OS cell lines and their resistant sublines ( n = 3). Data are represented as mean ± SD.
    Grp78 Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grp78 shrna lentiviral particles/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    grp78 shrna lentiviral particles - by Bioz Stars, 2024-04
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    85
    DSMZ actinopolyspora mortivallis h16 cultures
    ( A ) U-2 OS and MG-63 cells were treated with conventional chemotherapeutic drugs in the concentration of their IC 50 values for OS cells for 24 hr and detected by qRT-PCR. The Δ cycle threshold method was used for the calculation of relative differences in mRNA abundance. Data were normalized to the expression of GAPDH . The results of real-time RT-PCR were expressed as fold-changes. The normalized value of the target mRNA of the control group is arbitrarily presented as 1 ( n = 6). ( B ) U-2 OS and MG-63 cells were treated with 1 μM DOX for 0 to 72 hr. The expression of P-gp at the membrane surface and the levels of <t>GRP78,</t> p-Akt and Akt in the whole-cell lysates were detected by Western blot and normalized by GAPDH ( n = 3). ( C ) Nude mice bearing xenograft tumors were treated with DOX by intraperitoneal injection once every four days. Three mice were selected randomly and sacrificed every day. Xenografts were removed, fixed and paraffin embedded. IHC staining was performed by using P-gp, GRP78 and p-Akt antibodies (×400) ( n = 6). ( D ) Western blot results of the membrane lysates or total lysates from OS cell lines and their resistant sublines ( n = 3). Data are represented as mean ± SD.
    Actinopolyspora Mortivallis H16 Cultures, supplied by DSMZ, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/actinopolyspora mortivallis h16 cultures/product/DSMZ
    Average 85 stars, based on 1 article reviews
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    Image Search Results


    Dox inhibited DT-010-induced GRP78 expression in MCF-7 cells. Immunoblots (A) and densitometric analysis (C) of the expression of GRP78 protein were determined after DT-010 treatment in MCF-7 cells. (B) MCF-7 cells were treated with DT-010 for 24 h in the presence or absence of Dox. Representative immunoblots for the expression of GRP78 and β-actin proteins were exhibited. (D) Densitometric analysis showed the expression of GRP78 protein. # P < 0.05 vs. Ctrl.

    Journal: Frontiers in Pharmacology

    Article Title: A Novel Agent Enhances the Chemotherapeutic Efficacy of Doxorubicin in MCF-7 Breast Cancer Cells

    doi: 10.3389/fphar.2016.00249

    Figure Lengend Snippet: Dox inhibited DT-010-induced GRP78 expression in MCF-7 cells. Immunoblots (A) and densitometric analysis (C) of the expression of GRP78 protein were determined after DT-010 treatment in MCF-7 cells. (B) MCF-7 cells were treated with DT-010 for 24 h in the presence or absence of Dox. Representative immunoblots for the expression of GRP78 and β-actin proteins were exhibited. (D) Densitometric analysis showed the expression of GRP78 protein. # P < 0.05 vs. Ctrl.

    Article Snippet: GRP78 siRNA was provided by Santa Cruz Biotechnology (USA).

    Techniques: Expressing, Western Blot

    Inhibition of GRP78 expression enhanced DT-010-induced cell apoptosis in MCF-7 cells. (A) The percentage of apoptotic cells in MCF-7 cells was recorded after 24 h of DT-010 treatment in the presence or absence of ER stress inhibitor 4-PBA. # P < 0.05 vs. DT-010 treated group. (B) MCF-7 cells were treated with Dox and DT-010 for 24 h, the expression of p53, cleaved-PARP, β-actin proteins were measured by western blot. The expression of GRP78 protein was determined after 12 h of Dox and DT-010 treatment. Representative immunoblots for the expression of p53, cleaved-PARP, β-actin and GRP78 proteins. (C) Densitometric quantification of the expression of GRP78 protein. (D) GRP78 knockdown further promotes DT-010-induced cell death in MCF-7 cells. ∗ means significant difference between treatments ( P < 0.05).

    Journal: Frontiers in Pharmacology

    Article Title: A Novel Agent Enhances the Chemotherapeutic Efficacy of Doxorubicin in MCF-7 Breast Cancer Cells

    doi: 10.3389/fphar.2016.00249

    Figure Lengend Snippet: Inhibition of GRP78 expression enhanced DT-010-induced cell apoptosis in MCF-7 cells. (A) The percentage of apoptotic cells in MCF-7 cells was recorded after 24 h of DT-010 treatment in the presence or absence of ER stress inhibitor 4-PBA. # P < 0.05 vs. DT-010 treated group. (B) MCF-7 cells were treated with Dox and DT-010 for 24 h, the expression of p53, cleaved-PARP, β-actin proteins were measured by western blot. The expression of GRP78 protein was determined after 12 h of Dox and DT-010 treatment. Representative immunoblots for the expression of p53, cleaved-PARP, β-actin and GRP78 proteins. (C) Densitometric quantification of the expression of GRP78 protein. (D) GRP78 knockdown further promotes DT-010-induced cell death in MCF-7 cells. ∗ means significant difference between treatments ( P < 0.05).

    Article Snippet: GRP78 siRNA was provided by Santa Cruz Biotechnology (USA).

    Techniques: Inhibition, Expressing, Western Blot

    ( A ) U-2 OS and MG-63 cells were treated with conventional chemotherapeutic drugs in the concentration of their IC 50 values for OS cells for 24 hr and detected by qRT-PCR. The Δ cycle threshold method was used for the calculation of relative differences in mRNA abundance. Data were normalized to the expression of GAPDH . The results of real-time RT-PCR were expressed as fold-changes. The normalized value of the target mRNA of the control group is arbitrarily presented as 1 ( n = 6). ( B ) U-2 OS and MG-63 cells were treated with 1 μM DOX for 0 to 72 hr. The expression of P-gp at the membrane surface and the levels of GRP78, p-Akt and Akt in the whole-cell lysates were detected by Western blot and normalized by GAPDH ( n = 3). ( C ) Nude mice bearing xenograft tumors were treated with DOX by intraperitoneal injection once every four days. Three mice were selected randomly and sacrificed every day. Xenografts were removed, fixed and paraffin embedded. IHC staining was performed by using P-gp, GRP78 and p-Akt antibodies (×400) ( n = 6). ( D ) Western blot results of the membrane lysates or total lysates from OS cell lines and their resistant sublines ( n = 3). Data are represented as mean ± SD.

    Journal: Oncotarget

    Article Title: Combining GRP78 suppression and MK2206-induced Akt inhibition decreases doxorubicin-induced P-glycoprotein expression and mitigates chemoresistance in human osteosarcoma

    doi: 10.18632/oncotarget.10890

    Figure Lengend Snippet: ( A ) U-2 OS and MG-63 cells were treated with conventional chemotherapeutic drugs in the concentration of their IC 50 values for OS cells for 24 hr and detected by qRT-PCR. The Δ cycle threshold method was used for the calculation of relative differences in mRNA abundance. Data were normalized to the expression of GAPDH . The results of real-time RT-PCR were expressed as fold-changes. The normalized value of the target mRNA of the control group is arbitrarily presented as 1 ( n = 6). ( B ) U-2 OS and MG-63 cells were treated with 1 μM DOX for 0 to 72 hr. The expression of P-gp at the membrane surface and the levels of GRP78, p-Akt and Akt in the whole-cell lysates were detected by Western blot and normalized by GAPDH ( n = 3). ( C ) Nude mice bearing xenograft tumors were treated with DOX by intraperitoneal injection once every four days. Three mice were selected randomly and sacrificed every day. Xenografts were removed, fixed and paraffin embedded. IHC staining was performed by using P-gp, GRP78 and p-Akt antibodies (×400) ( n = 6). ( D ) Western blot results of the membrane lysates or total lysates from OS cell lines and their resistant sublines ( n = 3). Data are represented as mean ± SD.

    Article Snippet: GRP78 shRNA lentiviral particles (sc-44261-V, Santa Cruz Biotechnology) is a pool of concentrated, transduction-ready viral particles containing 3 target-specific constructs that encode 19–25 nt shRNA designed to knock down gene expression; control shRNA (sc-108080, Santa Cruz Biotechnology) is recommended by Santa Cruz Biotechnology as a negative control for GRP78 knockdown.

    Techniques: Concentration Assay, Quantitative RT-PCR, Expressing, Western Blot, Injection, Immunohistochemistry

    ( A ) U-2 OS or MG-63 cells were seeded into 12-well culture plates at 0.5 × 10 5 cells per well overnight. Subsequently, the cells were pretreated with or without 1 mL of complete media containing 5 μg/mL Polybrene for 5-min. The cells were treated with or without shRNA lentiviral particles for GRP78 or control for 24 hr. Then fresh media without Polybrene was placed and cells were treated with 5 nM DOX and/or 30 nM MK2206. When growth of the cells reached 90% confluence, the cells were harvested and reseeded at the density of 0.5 × 10 5 cells/well, and the DOX concentration increased. Three parallel experiments in each group were performed simultaneously. The experiments were carried out for 245 days ( n = 3). ( B ) The IC 50 values of DOX and MK2206 for each cell line shown in Figure . Cells were seeded into 96-well plates at the density of 3500 cells/well. The next day, concentration gradient of chemicals was added and cells were incubated for further 48 hr. MTT assay was performed ( n = 3). ( C ) Protein levels in the membrane lysates or total lysates of OS cells were detected by Western blot with GAPDH as loading control. OS parental sensitive cell lines were used as negative control ( n = 3). ( D and E ) MG-63 cells were infected with the lentiviral constructs. After replacing the fresh medium, cells were diluted to 1:3 for selecting stable clones expressing the shRNA by 5 μg/mL puromycin dihydrochloride. Four weeks later, resistant colonies were picked and expanded for xenograft experiments. Three million MG-63 cells were stably transfected with shGRP78, or control lentiviral particles were suspended in 1:2 PBS/Matrigel in a total volume of 200 μL and subcutaneously injected into the right forelimb of each mouse. When mean tumor volume reached approximately 100 mm 3 , 30 mice were randomized into five groups ( n = 6/group) with approximately equivalent ranges of tumor volume between groups. Mice were treated with MK2206 (60 mg/kg) formulated in 30% Captisol by oral gavage thrice a week. DOX (hydrochloride) (2 mg/kg) was administered by intraperitoneal injection once every 4 days. The control group was treated with 30% Captisol by oral gavage thrice a week. Tumor growth was measured with calipers every other day, and tumor volume was calculated using the following formula: volume (mm 3 ) = length × width 2 × 0.5. After 21 days, the animals were sacrificed, and xenografts were removed and weighed ( n = 6). Data are represented as mean ± SD.

    Journal: Oncotarget

    Article Title: Combining GRP78 suppression and MK2206-induced Akt inhibition decreases doxorubicin-induced P-glycoprotein expression and mitigates chemoresistance in human osteosarcoma

    doi: 10.18632/oncotarget.10890

    Figure Lengend Snippet: ( A ) U-2 OS or MG-63 cells were seeded into 12-well culture plates at 0.5 × 10 5 cells per well overnight. Subsequently, the cells were pretreated with or without 1 mL of complete media containing 5 μg/mL Polybrene for 5-min. The cells were treated with or without shRNA lentiviral particles for GRP78 or control for 24 hr. Then fresh media without Polybrene was placed and cells were treated with 5 nM DOX and/or 30 nM MK2206. When growth of the cells reached 90% confluence, the cells were harvested and reseeded at the density of 0.5 × 10 5 cells/well, and the DOX concentration increased. Three parallel experiments in each group were performed simultaneously. The experiments were carried out for 245 days ( n = 3). ( B ) The IC 50 values of DOX and MK2206 for each cell line shown in Figure . Cells were seeded into 96-well plates at the density of 3500 cells/well. The next day, concentration gradient of chemicals was added and cells were incubated for further 48 hr. MTT assay was performed ( n = 3). ( C ) Protein levels in the membrane lysates or total lysates of OS cells were detected by Western blot with GAPDH as loading control. OS parental sensitive cell lines were used as negative control ( n = 3). ( D and E ) MG-63 cells were infected with the lentiviral constructs. After replacing the fresh medium, cells were diluted to 1:3 for selecting stable clones expressing the shRNA by 5 μg/mL puromycin dihydrochloride. Four weeks later, resistant colonies were picked and expanded for xenograft experiments. Three million MG-63 cells were stably transfected with shGRP78, or control lentiviral particles were suspended in 1:2 PBS/Matrigel in a total volume of 200 μL and subcutaneously injected into the right forelimb of each mouse. When mean tumor volume reached approximately 100 mm 3 , 30 mice were randomized into five groups ( n = 6/group) with approximately equivalent ranges of tumor volume between groups. Mice were treated with MK2206 (60 mg/kg) formulated in 30% Captisol by oral gavage thrice a week. DOX (hydrochloride) (2 mg/kg) was administered by intraperitoneal injection once every 4 days. The control group was treated with 30% Captisol by oral gavage thrice a week. Tumor growth was measured with calipers every other day, and tumor volume was calculated using the following formula: volume (mm 3 ) = length × width 2 × 0.5. After 21 days, the animals were sacrificed, and xenografts were removed and weighed ( n = 6). Data are represented as mean ± SD.

    Article Snippet: GRP78 shRNA lentiviral particles (sc-44261-V, Santa Cruz Biotechnology) is a pool of concentrated, transduction-ready viral particles containing 3 target-specific constructs that encode 19–25 nt shRNA designed to knock down gene expression; control shRNA (sc-108080, Santa Cruz Biotechnology) is recommended by Santa Cruz Biotechnology as a negative control for GRP78 knockdown.

    Techniques: shRNA, Concentration Assay, Incubation, MTT Assay, Western Blot, Negative Control, Infection, Construct, Clone Assay, Expressing, Stable Transfection, Transfection, Injection