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Image Search Results

Journal: Epigenomes
Article Title: Modulation of DNA Methylation/Demethylation Reactions Induced by Nutraceuticals and Pollutants of Exposome Can Promote a C > T Mutation in the Breast Cancer Predisposing Gene PALB2
doi: 10.3390/epigenomes6040032
Figure Lengend Snippet: Diuron and PFOA supplementation affect APOBEC expression and TDG activity, respectively. ( A ) Impact of Diuron on the APOBEC3α and APOBEC3γ expression (ELISA method). Graph illustrates the fold change expression of APOBEC3α and APOBEC3γ expression seen in MCF10A cells exposed to Diuron at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO 5%. siRNA-A (control) and siRNA-APOBECs are used as control conditions inducing down-expression of APOBEC3α and APOBEC3γ. A significant difference (*) was observed between the different doses of Diuron used. ( B ) Impact of PFOA on TDG activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to PFOA at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO5%. A significant difference (*) was observed between the different doses of PFOA used.
Article Snippet: siRNA-APOBEC3γ (sc-60091, Santa Cruz, Heidelberg, Germany), siRNA-APOBEC3α (sc-72514, Santa Cruz, Heidelberg, Germany),
Techniques: Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay

Journal: Epigenomes
Article Title: Modulation of DNA Methylation/Demethylation Reactions Induced by Nutraceuticals and Pollutants of Exposome Can Promote a C > T Mutation in the Breast Cancer Predisposing Gene PALB2
doi: 10.3390/epigenomes6040032
Figure Lengend Snippet: Ascorbic acid and Iron have the ability to limit the presence of the c.1027C > T mutation in PALB2 gene. ( A ) Impact of Glyphosate on TET3 expression (ELISA method). Graph illustrates the fold change expression of TET3 expression seen in MCF10A cells exposed to Glyphosate at different doses (MAC and multiple of MAC). “Ctrl” represents the control exposure performed with PBS/DMSO 5%. siRNA-A (control) and siRNA-TET3 are used as control conditions inducing down-expression of TET3. A significant difference (*) was observed between the different doses of glyphosate used. ( B ) Impact of Ascorbic Acid or Iron on TET activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to Ascorbic Acid or Iron at different doses (RDI and multiple of RDI). “Ctrl” represents the control exposure performed with PBS/DMSO 5%. DMOG was used as control condition inducing a decrease in TET activity since DMOG acts as a TET inhibitor. A significant difference (*) was observed between the different doses of ascorbic acid and iron used. ( C ) Graphs illustrate the Impact of nutraceuticals and pollutants as single agent or in a mixture on the presence of the c.1027C > T mutation in PALB2 gene (Restriction site mutation (RSM) method). Doses used are indicated in multiples of RDI or MAC. A significant difference (*) was observed between the indicated comparisons.
Article Snippet: siRNA-APOBEC3γ (sc-60091, Santa Cruz, Heidelberg, Germany), siRNA-APOBEC3α (sc-72514, Santa Cruz, Heidelberg, Germany),
Techniques: Mutagenesis, Expressing, Enzyme-linked Immunosorbent Assay, Activity Assay