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  • 90
    ATCC burkholderia vietnamiensis g4 yp 001110606 monomeric at zp 02384278
    Burkholderia Vietnamiensis G4 Yp 001110606 Monomeric At Zp 02384278, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology sirna tdg
    Diuron and PFOA supplementation affect APOBEC expression and <t>TDG</t> activity, respectively. ( A ) Impact of Diuron on the APOBEC3α and APOBEC3γ expression (ELISA method). Graph illustrates the fold change expression of APOBEC3α and APOBEC3γ expression seen in MCF10A cells exposed to Diuron at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO 5%. <t>siRNA-A</t> (control) and siRNA-APOBECs are used as control conditions inducing down-expression of APOBEC3α and APOBEC3γ. A significant difference (*) was observed between the different doses of Diuron used. ( B ) Impact of PFOA on TDG activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to PFOA at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO5%. A significant difference (*) was observed between the different doses of PFOA used.
    Sirna Tdg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    DSMZ tsukamurella species
    Diuron and PFOA supplementation affect APOBEC expression and <t>TDG</t> activity, respectively. ( A ) Impact of Diuron on the APOBEC3α and APOBEC3γ expression (ELISA method). Graph illustrates the fold change expression of APOBEC3α and APOBEC3γ expression seen in MCF10A cells exposed to Diuron at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO 5%. <t>siRNA-A</t> (control) and siRNA-APOBECs are used as control conditions inducing down-expression of APOBEC3α and APOBEC3γ. A significant difference (*) was observed between the different doses of Diuron used. ( B ) Impact of PFOA on TDG activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to PFOA at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO5%. A significant difference (*) was observed between the different doses of PFOA used.
    Tsukamurella Species, supplied by DSMZ, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Broadcom Corporation wo00 44142
    Diuron and PFOA supplementation affect APOBEC expression and <t>TDG</t> activity, respectively. ( A ) Impact of Diuron on the APOBEC3α and APOBEC3γ expression (ELISA method). Graph illustrates the fold change expression of APOBEC3α and APOBEC3γ expression seen in MCF10A cells exposed to Diuron at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO 5%. <t>siRNA-A</t> (control) and siRNA-APOBECs are used as control conditions inducing down-expression of APOBEC3α and APOBEC3γ. A significant difference (*) was observed between the different doses of Diuron used. ( B ) Impact of PFOA on TDG activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to PFOA at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO5%. A significant difference (*) was observed between the different doses of PFOA used.
    Wo00 44142, supplied by Broadcom Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Neocera Inc oh 44142 neocera
    Diuron and PFOA supplementation affect APOBEC expression and <t>TDG</t> activity, respectively. ( A ) Impact of Diuron on the APOBEC3α and APOBEC3γ expression (ELISA method). Graph illustrates the fold change expression of APOBEC3α and APOBEC3γ expression seen in MCF10A cells exposed to Diuron at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO 5%. <t>siRNA-A</t> (control) and siRNA-APOBECs are used as control conditions inducing down-expression of APOBEC3α and APOBEC3γ. A significant difference (*) was observed between the different doses of Diuron used. ( B ) Impact of PFOA on TDG activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to PFOA at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO5%. A significant difference (*) was observed between the different doses of PFOA used.
    Oh 44142 Neocera, supplied by Neocera Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Diuron and PFOA supplementation affect APOBEC expression and TDG activity, respectively. ( A ) Impact of Diuron on the APOBEC3α and APOBEC3γ expression (ELISA method). Graph illustrates the fold change expression of APOBEC3α and APOBEC3γ expression seen in MCF10A cells exposed to Diuron at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO 5%. siRNA-A (control) and siRNA-APOBECs are used as control conditions inducing down-expression of APOBEC3α and APOBEC3γ. A significant difference (*) was observed between the different doses of Diuron used. ( B ) Impact of PFOA on TDG activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to PFOA at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO5%. A significant difference (*) was observed between the different doses of PFOA used.

    Journal: Epigenomes

    Article Title: Modulation of DNA Methylation/Demethylation Reactions Induced by Nutraceuticals and Pollutants of Exposome Can Promote a C > T Mutation in the Breast Cancer Predisposing Gene PALB2

    doi: 10.3390/epigenomes6040032

    Figure Lengend Snippet: Diuron and PFOA supplementation affect APOBEC expression and TDG activity, respectively. ( A ) Impact of Diuron on the APOBEC3α and APOBEC3γ expression (ELISA method). Graph illustrates the fold change expression of APOBEC3α and APOBEC3γ expression seen in MCF10A cells exposed to Diuron at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO 5%. siRNA-A (control) and siRNA-APOBECs are used as control conditions inducing down-expression of APOBEC3α and APOBEC3γ. A significant difference (*) was observed between the different doses of Diuron used. ( B ) Impact of PFOA on TDG activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to PFOA at different doses (MAC and multiple of MAC). ”Ctrl” represents the control exposure performed with PBS/DMSO5%. A significant difference (*) was observed between the different doses of PFOA used.

    Article Snippet: siRNA-APOBEC3γ (sc-60091, Santa Cruz, Heidelberg, Germany), siRNA-APOBEC3α (sc-72514, Santa Cruz, Heidelberg, Germany), siRNA-TDG (sc-44142, Santa Cruz, France), and siRNA-TET3 (sc-154206, Santa Cruz, Heidelberg, Germany) were transfected in MCF10A as previously described (Duforestel et al. 2019). siRNA-A (sc-37007, Santa Cruz, Heidelberg, Germany), that is, a scrambled sequence devoid of specific degradation of any cellular message was used as control.

    Techniques: Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay

    Ascorbic acid and Iron have the ability to limit the presence of the c.1027C > T mutation in PALB2 gene. ( A ) Impact of Glyphosate on TET3 expression (ELISA method). Graph illustrates the fold change expression of TET3 expression seen in MCF10A cells exposed to Glyphosate at different doses (MAC and multiple of MAC). “Ctrl” represents the control exposure performed with PBS/DMSO 5%. siRNA-A (control) and siRNA-TET3 are used as control conditions inducing down-expression of TET3. A significant difference (*) was observed between the different doses of glyphosate used. ( B ) Impact of Ascorbic Acid or Iron on TET activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to Ascorbic Acid or Iron at different doses (RDI and multiple of RDI). “Ctrl” represents the control exposure performed with PBS/DMSO 5%. DMOG was used as control condition inducing a decrease in TET activity since DMOG acts as a TET inhibitor. A significant difference (*) was observed between the different doses of ascorbic acid and iron used. ( C ) Graphs illustrate the Impact of nutraceuticals and pollutants as single agent or in a mixture on the presence of the c.1027C > T mutation in PALB2 gene (Restriction site mutation (RSM) method). Doses used are indicated in multiples of RDI or MAC. A significant difference (*) was observed between the indicated comparisons.

    Journal: Epigenomes

    Article Title: Modulation of DNA Methylation/Demethylation Reactions Induced by Nutraceuticals and Pollutants of Exposome Can Promote a C > T Mutation in the Breast Cancer Predisposing Gene PALB2

    doi: 10.3390/epigenomes6040032

    Figure Lengend Snippet: Ascorbic acid and Iron have the ability to limit the presence of the c.1027C > T mutation in PALB2 gene. ( A ) Impact of Glyphosate on TET3 expression (ELISA method). Graph illustrates the fold change expression of TET3 expression seen in MCF10A cells exposed to Glyphosate at different doses (MAC and multiple of MAC). “Ctrl” represents the control exposure performed with PBS/DMSO 5%. siRNA-A (control) and siRNA-TET3 are used as control conditions inducing down-expression of TET3. A significant difference (*) was observed between the different doses of glyphosate used. ( B ) Impact of Ascorbic Acid or Iron on TET activity (Activity assay method). Graph illustrates the fold change of TDG activity seen in MCF10A cells exposed to Ascorbic Acid or Iron at different doses (RDI and multiple of RDI). “Ctrl” represents the control exposure performed with PBS/DMSO 5%. DMOG was used as control condition inducing a decrease in TET activity since DMOG acts as a TET inhibitor. A significant difference (*) was observed between the different doses of ascorbic acid and iron used. ( C ) Graphs illustrate the Impact of nutraceuticals and pollutants as single agent or in a mixture on the presence of the c.1027C > T mutation in PALB2 gene (Restriction site mutation (RSM) method). Doses used are indicated in multiples of RDI or MAC. A significant difference (*) was observed between the indicated comparisons.

    Article Snippet: siRNA-APOBEC3γ (sc-60091, Santa Cruz, Heidelberg, Germany), siRNA-APOBEC3α (sc-72514, Santa Cruz, Heidelberg, Germany), siRNA-TDG (sc-44142, Santa Cruz, France), and siRNA-TET3 (sc-154206, Santa Cruz, Heidelberg, Germany) were transfected in MCF10A as previously described (Duforestel et al. 2019). siRNA-A (sc-37007, Santa Cruz, Heidelberg, Germany), that is, a scrambled sequence devoid of specific degradation of any cellular message was used as control.

    Techniques: Mutagenesis, Expressing, Enzyme-linked Immunosorbent Assay, Activity Assay