Journal: Psychopharmacology

Article Title: Perseveration in a spatial-discrimination serial reversal learning task is differentially affected by MAO-A and MAO-B inhibition and associated with reduced anxiety and peripheral serotonin levels

doi: 10.1007/s00213-017-4569-x

Figure Lengend Snippet: Effects of selective MAO inhibition on monoamine levels in a OFC, b DRN, c BLA, and d dorsomedial striatum (pmol/mg tissue). In c and d , dopamine levels are shown on the left y -axis while NA and 5-HT levels are shown on the right y -axis. Data are mean values ± SEM. Significance is denoted as follows: * p < 0.05, ** p < 0.005 versus vehicle, + p < 0.05 versus lazabemide. e Coronal sections showing regions of interest for ex vivo neurochemical analysis of monoamines following vehicle, moclobemide, and lazabemide administration. dmPFC dorsomedial PFC, OFC orbitofrontal cortex, dmS dorsomedial striatum, NAcb nucleus accumbens, BLA basolateral amygdala, CA1 hippocampal CA1 region, LH lateral hypothalamus, DRN dorsal raphé nuclei. Adapted from Paxinos and Watson

Article Snippet: Moclobemide and lazabemide hydrochloride were purchased from Tocris (UK) and dissolved in 15% hydroxypropyl-beta-cyclodextrin and 0.9% saline (“vehicle”).

Techniques: Inhibition, Ex Vivo

Journal: Psychopharmacology

Article Title: Perseveration in a spatial-discrimination serial reversal learning task is differentially affected by MAO-A and MAO-B inhibition and associated with reduced anxiety and peripheral serotonin levels

doi: 10.1007/s00213-017-4569-x

Figure Lengend Snippet: Final group sizes for animals that successfully completed the task under drug conditions

Article Snippet: Moclobemide and lazabemide hydrochloride were purchased from Tocris (UK) and dissolved in 15% hydroxypropyl-beta-cyclodextrin and 0.9% saline (“vehicle”).

Techniques:

Journal: Psychopharmacology

Article Title: Perseveration in a spatial-discrimination serial reversal learning task is differentially affected by MAO-A and MAO-B inhibition and associated with reduced anxiety and peripheral serotonin levels

doi: 10.1007/s00213-017-4569-x

Figure Lengend Snippet: Effects of moclobemide ( n = 18) and lazabemide ( n = 21) on total trials to achieve criterion ( a , b ) and the proportion of perseverative errors ( c , d ). Mean values ± SEM for a single post drug administration session are shown. Significance is denoted as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle

Article Snippet: Moclobemide and lazabemide hydrochloride were purchased from Tocris (UK) and dissolved in 15% hydroxypropyl-beta-cyclodextrin and 0.9% saline (“vehicle”).

Techniques:

Journal: Psychopharmacology

Article Title: Perseveration in a spatial-discrimination serial reversal learning task is differentially affected by MAO-A and MAO-B inhibition and associated with reduced anxiety and peripheral serotonin levels

doi: 10.1007/s00213-017-4569-x

Figure Lengend Snippet: Effects of moclobemide ( n = 16) and lazabemide ( n = 20) on response latencies (s) following an incorrect ( a , b ) and correct ( c , d ) response. Data for two animals was not saved due to a technical failure with the equipment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle

Article Snippet: Moclobemide and lazabemide hydrochloride were purchased from Tocris (UK) and dissolved in 15% hydroxypropyl-beta-cyclodextrin and 0.9% saline (“vehicle”).

Techniques:

Journal: Psychopharmacology

Article Title: Perseveration in a spatial-discrimination serial reversal learning task is differentially affected by MAO-A and MAO-B inhibition and associated with reduced anxiety and peripheral serotonin levels

doi: 10.1007/s00213-017-4569-x

Figure Lengend Snippet: Lose-shift probabilities for high ( n = 5) and low ( n = 5) perseveration rats that received moclobemide (16 mg/kg, 3 mg/kg), combination of lazabemide and moclobemide, and vehicle

Article Snippet: Moclobemide and lazabemide hydrochloride were purchased from Tocris (UK) and dissolved in 15% hydroxypropyl-beta-cyclodextrin and 0.9% saline (“vehicle”).

Techniques:

Journal: Psychopharmacology

Article Title: Perseveration in a spatial-discrimination serial reversal learning task is differentially affected by MAO-A and MAO-B inhibition and associated with reduced anxiety and peripheral serotonin levels

doi: 10.1007/s00213-017-4569-x

Figure Lengend Snippet: Levels of DA and 5-HT in regions of interest following vehicle (Veh, n = 5), lazabemide (L10, n = 6), and moclobemide (M3, n = 4; M16, n = 4) administration

Article Snippet: Moclobemide and lazabemide hydrochloride were purchased from Tocris (UK) and dissolved in 15% hydroxypropyl-beta-cyclodextrin and 0.9% saline (“vehicle”).

Techniques:

Journal: Acta Pharmacologica Sinica

Article Title: Irisin exerts a therapeutic effect against myocardial infarction via promoting angiogenesis

doi: 10.1038/s41401-019-0230-z

Figure Lengend Snippet: The pro-angiogenic effect of irisin was associated with the ERK signaling pathway. a Representative images and quantification of phosphorylated and total ERK protein expression in HUVEC analyzed by Western blotting. The ERK inhibitor U0126 was used to block ERK phosphorylation, and β-actin was used as the internal control. n = 5. b Representative images and quantification of phosphorylated and total ERK protein expression in MI mice analyzed by Western blotting. n = 5. c Representative images and quantification of HUVEC migration detected by transwell migration assay. n = 3, scale bar = 100 μm. d Representative images and quantification of HUVEC migration detected by scratch wound-healing assay. n = 3, scale bar = 100 μm. *P < 0.05 vs. control

Article Snippet: After treatment with blocking buffer, the blots were probed with the anti-phospho ERK antibody (1:1000, Cat. No. 4370 S, Cell Signaling Technology), anti-ERK antibody (1:1000, Cat. No. 4395 S, Cell Signaling Technology) and β-actin antibody (1:600, Santa Cruz Biotechnology, CA) at 4 °C overnight.

Techniques: Expressing, Western Blot, Blocking Assay, Phospho-proteomics, Control, Migration, Transwell Migration Assay, Wound Healing Assay

Journal: Microbes and environments

Article Title: Comprehensive detection of phototrophic sulfur bacteria using PCR primers that target reverse dissimilatory sulfite reductase gene.

doi: 10.1264/jsme2.me10109

Figure Lengend Snippet: Fig. 1. PCR amplification of 16S rRNA gene from representative phototrophic sulfur bacteria, and sulfur-oxidizing, sulfate-reducing, and heterotrophic bacteria as controls using a set of bacterial universal primers (A) and of dsrA using PGdsrAF and PGdsrAR specific to phototrophic sulfur bacteria (B) analyzed by 1% (w/v) agarose gel electrophoresis in 1× TAE stained with ethidium bromide. Lanes: M, EZ LoadTM 100 bp PCR Molecular Ruler (Bio-Rad); 1, Allochromatium vinosum DSM 180T; 2, Halochromatium salexigens DSM 4395T; 3, Halorhodospira halophila DSM 244T; 4, Thiobaca trueperi DSM 13587T; 5, Thiocapsa marina DSM 5653T; 6, Thiococcus pfennigii DSM 226; 7, Thiorhodococcus drewsii DSM 15006T; 8, Chlorobium limicola DSM 245T; 9, Chlorobium. luteolum DSM 273T; 10, Chlorobium. phaeovibrioides DSM 269T; 11, Prosthecochloris aestuarii DSM 272; 12, P. vibrioformis DSM 261; 13, Chloroflexus aurantiacus DSM 635T; 14, Heliobacterium chlorum DSM 3682T; 15, Thiobacillus denitrificans DSM 12475T; 16, Thiomicrospira pelophila DSM 1534T; 17, Desulfotomaculum geothermicum DSM 3669T; 18, Desulfovibrio desulfuricans DSM 642T; 19, Desulfovibrio africanus DSM 2603T; 20, Desulfomicrobium norvegicum DSM 1741T; 21, Desulfobacter postgatei DSM 2034T; 22, Desulfobulbus sp. DSM 2033; 23, Desulfobulbus propionicus DSM 2032T; 24, Desulfosarcina variabilis DSM 2060T; 25, Desulfosporocinas orientis DSM 765T; 26, Alteromonas macleodii IAM 12920T; 27, Shewanella putrefaciens IAM 1509T; 28, Flavobacterium aquatile IAM 12316T; 29, Cytophaga matinoflava IAM 14170T; and C, no template control.

Article Snippet: The following strains of phototrophic sulfur bacteria were obtained from Deutsch Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany) either as lyophilized or actively growing cultures: Allochromatium vinosum DSM 180T, Halorhodospira halophila DSM 244T, Halochromatium salexigens DSM 4395T, Thiobaca trueperi DSM 13587T, Thiocapsa marina DSM 5653T, Thiorhodococcus drewsii DSM 15006, Marichromatium gracile DSM 203T, Thiococcus pfennigii DSM 266, Prosthecochloris vibrioformis DSM 261 Chlorobium limicola DSM 245T, Chlorobium luteolum DSM 273T, Prosthecochloris aestuarii DSM 271T, and Chlorobium phaeovibrioides DSM 269T.

Techniques: Amplification, Bacteria, Agarose Gel Electrophoresis, Staining, Control