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    Human 43892 Protein Lysate 20ug from Innovative Research is provided as a Lyophilized powder. This is a Recombinant Protein Lysate produced in HEK293T cells. This protein lysate can be reconsituted using SDS Sample Buffer. Once
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    93
    ATCC enteroinvasive e coli atcc 43892
    IKK activation in response to enteroinvasive E. coli infection in wild-type Caco-2 cells and Caco-2 cells expressing DN Nod1. Control Caco-2, Caco-2 cells that express an empty vector (CEV1), and Caco-2 cells that stably express DN Nod1 (CDN10) cells were infected with enteroinvasive E. coli <t>O29:NM,</t> after which IKK activity was determined by an in vitro kinase assay at different times after infection by using a glutathione S-transferase-IκBα fusion protein as the substrate. IKKα levels assessed by immunoblotting revealed equal loading (data not shown).
    Enteroinvasive E Coli Atcc 43892, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enteroinvasive e coli atcc 43892/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enteroinvasive e coli atcc 43892 - by Bioz Stars, 2024-04
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    DSMZ name micromonospora ferruginea sp nov
    HPLC-ELSD chromatogram of the crude extract of <t>Micromonospora</t> sp. 28ISP2-46 T .
    Name Micromonospora Ferruginea Sp Nov, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    name micromonospora ferruginea sp nov - by Bioz Stars, 2024-04
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    86
    NCIMB Ltd no 43892
    HPLC-ELSD chromatogram of the crude extract of <t>Micromonospora</t> sp. 28ISP2-46 T .
    No 43892, supplied by NCIMB Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    no 43892 - by Bioz Stars, 2024-04
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    Standard format Plasmid sent in bacteria as agar stab
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    Image Search Results


    IKK activation in response to enteroinvasive E. coli infection in wild-type Caco-2 cells and Caco-2 cells expressing DN Nod1. Control Caco-2, Caco-2 cells that express an empty vector (CEV1), and Caco-2 cells that stably express DN Nod1 (CDN10) cells were infected with enteroinvasive E. coli O29:NM, after which IKK activity was determined by an in vitro kinase assay at different times after infection by using a glutathione S-transferase-IκBα fusion protein as the substrate. IKKα levels assessed by immunoblotting revealed equal loading (data not shown).

    Journal:

    Article Title: Nod1 Is an Essential Signal Transducer in Intestinal Epithelial Cells Infected with Bacteria That Avoid Recognition by Toll-Like Receptors

    doi: 10.1128/IAI.72.3.1487-1495.2004

    Figure Lengend Snippet: IKK activation in response to enteroinvasive E. coli infection in wild-type Caco-2 cells and Caco-2 cells expressing DN Nod1. Control Caco-2, Caco-2 cells that express an empty vector (CEV1), and Caco-2 cells that stably express DN Nod1 (CDN10) cells were infected with enteroinvasive E. coli O29:NM, after which IKK activity was determined by an in vitro kinase assay at different times after infection by using a glutathione S-transferase-IκBα fusion protein as the substrate. IKKα levels assessed by immunoblotting revealed equal loading (data not shown).

    Article Snippet: The following bacteria were used in these studies: enteroinvasive E. coli ATCC 43892 (serotype O29:NM), Shigella flexneri M90T ( 26 , 56 ), enterohemorrhagic E. coli 86-24 (serotype O157:H7) ( 4 ), and Salmonella enterica serovar Dublin strain Lane ( 39 ).

    Techniques: Activation Assay, Infection, Expressing, Plasmid Preparation, Stable Transfection, Activity Assay, In Vitro, Kinase Assay, Western Blot

    Inhibition of E. coli-induced NF-κB activation in cells that express DN Nod1. (Top panel) Caco-2 cell lines that stably express DN Nod1 (CDN1 and CDN10) or control vector (CEV1) were transiently transfected with a 3XNF-κB-luc reporter construct and either infected with enteroinvasive E. coli O29:NM (solid bars) or not infected (open bars). The data indicate the fold increases in luciferase activity compared to the luciferase activity of uninfected cells. The data are from three independent experiments, and the error bars indicate standard errors of the means. An asterisk indicates that the P value is <0.05 for a comparison with CEV1-infected cells. (Bottom panel) Myc expression was assessed by immunoblotting as an indicator of DN Nod1 expression in cell lysates from uninfected CEV1, CDN1, and CDN10 cells.

    Journal:

    Article Title: Nod1 Is an Essential Signal Transducer in Intestinal Epithelial Cells Infected with Bacteria That Avoid Recognition by Toll-Like Receptors

    doi: 10.1128/IAI.72.3.1487-1495.2004

    Figure Lengend Snippet: Inhibition of E. coli-induced NF-κB activation in cells that express DN Nod1. (Top panel) Caco-2 cell lines that stably express DN Nod1 (CDN1 and CDN10) or control vector (CEV1) were transiently transfected with a 3XNF-κB-luc reporter construct and either infected with enteroinvasive E. coli O29:NM (solid bars) or not infected (open bars). The data indicate the fold increases in luciferase activity compared to the luciferase activity of uninfected cells. The data are from three independent experiments, and the error bars indicate standard errors of the means. An asterisk indicates that the P value is <0.05 for a comparison with CEV1-infected cells. (Bottom panel) Myc expression was assessed by immunoblotting as an indicator of DN Nod1 expression in cell lysates from uninfected CEV1, CDN1, and CDN10 cells.

    Article Snippet: The following bacteria were used in these studies: enteroinvasive E. coli ATCC 43892 (serotype O29:NM), Shigella flexneri M90T ( 26 , 56 ), enterohemorrhagic E. coli 86-24 (serotype O157:H7) ( 4 ), and Salmonella enterica serovar Dublin strain Lane ( 39 ).

    Techniques: Inhibition, Activation Assay, Stable Transfection, Plasmid Preparation, Transfection, Construct, Infection, Luciferase, Activity Assay, Expressing, Western Blot

    RelA/p65 immunostaining of control and E. coli-infected cells. Control Caco-2 cells (top row), Caco-2 cells stably transfected with an empty vector (CEV1) (middle row), and Caco-2 cells stably transfected with DN Nod1 (CDN10) (bottom row) were not infected (left column) or were infected with enteroinvasive E. coli O29:NM (middle column) for 45 min and then immunostained with anti RelA/p65 antibody. Staining with an isotype control antibody is shown in the right column.

    Journal:

    Article Title: Nod1 Is an Essential Signal Transducer in Intestinal Epithelial Cells Infected with Bacteria That Avoid Recognition by Toll-Like Receptors

    doi: 10.1128/IAI.72.3.1487-1495.2004

    Figure Lengend Snippet: RelA/p65 immunostaining of control and E. coli-infected cells. Control Caco-2 cells (top row), Caco-2 cells stably transfected with an empty vector (CEV1) (middle row), and Caco-2 cells stably transfected with DN Nod1 (CDN10) (bottom row) were not infected (left column) or were infected with enteroinvasive E. coli O29:NM (middle column) for 45 min and then immunostained with anti RelA/p65 antibody. Staining with an isotype control antibody is shown in the right column.

    Article Snippet: The following bacteria were used in these studies: enteroinvasive E. coli ATCC 43892 (serotype O29:NM), Shigella flexneri M90T ( 26 , 56 ), enterohemorrhagic E. coli 86-24 (serotype O157:H7) ( 4 ), and Salmonella enterica serovar Dublin strain Lane ( 39 ).

    Techniques: Immunostaining, Infection, Stable Transfection, Transfection, Plasmid Preparation, Staining

    DN Nod1 does not eliminate NF-κB activation in response to stimulation with IL-1 or bacterial flagellin. (A) Control Caco-2 and CDN10 cells were infected with enteroinvasive E. coli O29:NM as described in Materials and Methods or stimulated with IL-1α (20 ng/ml) or were not infected or stimulated (control lanes). NF-κB DNA binding was assessed by an EMSA 45 min after infection or stimulation. (B) Caco-2 and CDN10 cells were stimulated with H7 flagellin (100 ng/ml) for different times or were not stimulated for 60 min, after which NF-κB DNA binding was determined by an EMSA.

    Journal:

    Article Title: Nod1 Is an Essential Signal Transducer in Intestinal Epithelial Cells Infected with Bacteria That Avoid Recognition by Toll-Like Receptors

    doi: 10.1128/IAI.72.3.1487-1495.2004

    Figure Lengend Snippet: DN Nod1 does not eliminate NF-κB activation in response to stimulation with IL-1 or bacterial flagellin. (A) Control Caco-2 and CDN10 cells were infected with enteroinvasive E. coli O29:NM as described in Materials and Methods or stimulated with IL-1α (20 ng/ml) or were not infected or stimulated (control lanes). NF-κB DNA binding was assessed by an EMSA 45 min after infection or stimulation. (B) Caco-2 and CDN10 cells were stimulated with H7 flagellin (100 ng/ml) for different times or were not stimulated for 60 min, after which NF-κB DNA binding was determined by an EMSA.

    Article Snippet: The following bacteria were used in these studies: enteroinvasive E. coli ATCC 43892 (serotype O29:NM), Shigella flexneri M90T ( 26 , 56 ), enterohemorrhagic E. coli 86-24 (serotype O157:H7) ( 4 ), and Salmonella enterica serovar Dublin strain Lane ( 39 ).

    Techniques: Activation Assay, Infection, Binding Assay

    NF-κB DNA binding in E. coli-infected Caco-2 cells. Caco-2 cells were not infected or were infected with E. coli O29:NM. The specificity of NF-κB DNA binding was assessed by using an excess of a specific oligonucleotide competitor (100× NF-κB) or a nonspecific oligonucleotide competitor (100× AP-1). Supershifts obtained with antibodies to RelB, cRel, p52, p50, and RelA/p65 are indicated on the right.

    Journal:

    Article Title: Nod1 Is an Essential Signal Transducer in Intestinal Epithelial Cells Infected with Bacteria That Avoid Recognition by Toll-Like Receptors

    doi: 10.1128/IAI.72.3.1487-1495.2004

    Figure Lengend Snippet: NF-κB DNA binding in E. coli-infected Caco-2 cells. Caco-2 cells were not infected or were infected with E. coli O29:NM. The specificity of NF-κB DNA binding was assessed by using an excess of a specific oligonucleotide competitor (100× NF-κB) or a nonspecific oligonucleotide competitor (100× AP-1). Supershifts obtained with antibodies to RelB, cRel, p52, p50, and RelA/p65 are indicated on the right.

    Article Snippet: The following bacteria were used in these studies: enteroinvasive E. coli ATCC 43892 (serotype O29:NM), Shigella flexneri M90T ( 26 , 56 ), enterohemorrhagic E. coli 86-24 (serotype O157:H7) ( 4 ), and Salmonella enterica serovar Dublin strain Lane ( 39 ).

    Techniques: Binding Assay, Infection

    DN Nod1 inhibits NF-κB target gene expression in E coli-infected Caco-2 cells. (A) CEV1, CDN1, and CDN10 cells were infected with enteroinvasive E. coli O29:NM. CXCL8 (open bars) and CXCL5 (solid bars) mRNA levels were assessed by real-time PCR 4 h after infection. The results are from three independent experiments, and the error bars indicate standard errors of the means. An asterisk indicates that the P value is <0.05 for a comparison with CEV1-infected cells. (B) CEV1 cells (open bars) and CDN10 cells (closed bars) were infected with E. coli O29:NM for 1 h, and CXCL8 secretion in culture supernatants was assessed at different times after infection. The results are from three or more independent experiments, and the error bars indicate standard errors of the means. An asterisk indicates that the P value is <0.05 for a comparison with CEV1-infected cells.

    Journal:

    Article Title: Nod1 Is an Essential Signal Transducer in Intestinal Epithelial Cells Infected with Bacteria That Avoid Recognition by Toll-Like Receptors

    doi: 10.1128/IAI.72.3.1487-1495.2004

    Figure Lengend Snippet: DN Nod1 inhibits NF-κB target gene expression in E coli-infected Caco-2 cells. (A) CEV1, CDN1, and CDN10 cells were infected with enteroinvasive E. coli O29:NM. CXCL8 (open bars) and CXCL5 (solid bars) mRNA levels were assessed by real-time PCR 4 h after infection. The results are from three independent experiments, and the error bars indicate standard errors of the means. An asterisk indicates that the P value is <0.05 for a comparison with CEV1-infected cells. (B) CEV1 cells (open bars) and CDN10 cells (closed bars) were infected with E. coli O29:NM for 1 h, and CXCL8 secretion in culture supernatants was assessed at different times after infection. The results are from three or more independent experiments, and the error bars indicate standard errors of the means. An asterisk indicates that the P value is <0.05 for a comparison with CEV1-infected cells.

    Article Snippet: The following bacteria were used in these studies: enteroinvasive E. coli ATCC 43892 (serotype O29:NM), Shigella flexneri M90T ( 26 , 56 ), enterohemorrhagic E. coli 86-24 (serotype O157:H7) ( 4 ), and Salmonella enterica serovar Dublin strain Lane ( 39 ).

    Techniques: Expressing, Infection, Real-time Polymerase Chain Reaction

    HPLC-ELSD chromatogram of the crude extract of Micromonospora sp. 28ISP2-46 T .

    Journal: Marine Drugs

    Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

    doi: 10.3390/md19020105

    Figure Lengend Snippet: HPLC-ELSD chromatogram of the crude extract of Micromonospora sp. 28ISP2-46 T .

    Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

    Techniques:

    Phylogenetic tree for strain 28ISP2-46 T and the other group IA Micromonospora species generated using the TYGS and drawn with iTOL. The tree was constructed using FastME 2.1.6.1 from GBDP distances calculated from genome sequences. The branch lengths are scaled in terms of GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values from 100 replications, with an average branch support of 98.4% and a delta statistic of 0.135. The tree was rooted using Micromonospora pallida as the outgroup.

    Journal: Marine Drugs

    Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

    doi: 10.3390/md19020105

    Figure Lengend Snippet: Phylogenetic tree for strain 28ISP2-46 T and the other group IA Micromonospora species generated using the TYGS and drawn with iTOL. The tree was constructed using FastME 2.1.6.1 from GBDP distances calculated from genome sequences. The branch lengths are scaled in terms of GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values from 100 replications, with an average branch support of 98.4% and a delta statistic of 0.135. The tree was rooted using Micromonospora pallida as the outgroup.

    Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

    Techniques: Generated, Construct

    Biosynthetic gene clusters identified in the genome of strain 28ISP2-46 T using AntiSMASH.

    Journal: Marine Drugs

    Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

    doi: 10.3390/md19020105

    Figure Lengend Snippet: Biosynthetic gene clusters identified in the genome of strain 28ISP2-46 T using AntiSMASH.

    Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

    Techniques:

    ( a ) ClusterBLAST hits for the kosinostatin cluster. Homologues of kst genes are coloured as follows: kstA genes red, kstB genes yellow, kstC genes green, kstD genes blue, kstRg genes black, kstRs genes white, and genes uninvolved in kosinostatin biosynthesis grey. A black line at the end of a cluster indicates that it is close to the end of a contig. Gene sizes not to scale. The NCBI protein IDs for first and last coloured genes of each cluster are as follows: Micromonospora sp. TP-A0468 AFJ52719.1 and AFJ52701.1; Micromonospora sp. 28ISP2-46 T QLQ36639.1 and QLQ36600.1; N. valliformis WP_017579213.1 and WP_017579258.1; N. alkaliphila WP_017604198.1 and WP_017604153.1; Streptomyces sp. SM8 PKA38649.1 and PKA38729.1; Streptomyces sp. ScaeMP-6W SCE31237.1 and SCE31955.1; A. bangkokensis OLR89622.1 and OLR89598.1; M. haikouensis SCF11188.1 and SCF11278.1. ( b ) The same gene clusters rearranged for ease of comparison. Coloured as 6a.

    Journal: Marine Drugs

    Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

    doi: 10.3390/md19020105

    Figure Lengend Snippet: ( a ) ClusterBLAST hits for the kosinostatin cluster. Homologues of kst genes are coloured as follows: kstA genes red, kstB genes yellow, kstC genes green, kstD genes blue, kstRg genes black, kstRs genes white, and genes uninvolved in kosinostatin biosynthesis grey. A black line at the end of a cluster indicates that it is close to the end of a contig. Gene sizes not to scale. The NCBI protein IDs for first and last coloured genes of each cluster are as follows: Micromonospora sp. TP-A0468 AFJ52719.1 and AFJ52701.1; Micromonospora sp. 28ISP2-46 T QLQ36639.1 and QLQ36600.1; N. valliformis WP_017579213.1 and WP_017579258.1; N. alkaliphila WP_017604198.1 and WP_017604153.1; Streptomyces sp. SM8 PKA38649.1 and PKA38729.1; Streptomyces sp. ScaeMP-6W SCE31237.1 and SCE31955.1; A. bangkokensis OLR89622.1 and OLR89598.1; M. haikouensis SCF11188.1 and SCF11278.1. ( b ) The same gene clusters rearranged for ease of comparison. Coloured as 6a.

    Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

    Techniques: