Journal: The Journal of Neuroscience

Article Title: Fragile X Mental Retardation Protein Bidirectionally Controls Dendritic I h in a Cell Type-Specific Manner between Mouse Hippocampus and Prefrontal Cortex

doi: 10.1523/JNEUROSCI.1670-19.2020

Figure Lengend Snippet: FMRP is in complex with HCN1 channel subunits. A, Western blot analysis of HCN1 immunoprecipitate identified associated HCN2, FMRP, and the indicated TRIP8b isoforms. B, Summary of group data shows that approximately threefold more FMRP was associated with HCN1 in the PFC compared with the HPC (three independent experiments, p = 0.0091). Other proteins were not significantly different across samples. Reported protein amounts were normalized to HCN1 in the precipitate. C, Lysates were prepared from the indicated brain regions of WT and fmr1–/y mice, and HCN proteins, TRIP8b isoforms, FMRP, and tubulin were quantified by Western blot (see Materials and Methods). Similar lysates were used for immunoprecipitation studies. D, Protein quantitation from group data normalized to tubulin. No significant differences in expression for any of the proteins could be discerned (three independent experiments for FMRP and tubulin; two experiments for HCN and TRIP8b proteins). ** p < 0.01.

Article Snippet: The membrane was blocked with 4% nonfat milk in PBST (10 mM PB, pH 7.4, 150 mM NaCl, 0.1% Tween 20) for 1 h and incubated at 4°C for 12 h in 1% milk in PBST with the appropriate primary antibody: anti-FMRP: 1:1000 (Cell Signaling Technology, #4317), anti-TRIP8b 1a-4 1:1000 (NeuroMab, #N212/3, RRID: AB_2750749 ), anti-TRIP8b 1a 1:1000 (NeuroMab, #N291C/22, RRID: AB_2315949 ), anti-HCN1 1:4 (NeuroMab, #73-110), anti-HCN2 1:200 (Alomone Labs, #APC-030), and anti-tubulin 1:5000 (Abcam, #EP1332Y).

Techniques: Western Blot, Immunoprecipitation, Protein Quantitation, Expressing

Journal: eLife

Article Title: IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate

doi: 10.7554/eLife.35074

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , Streptavidin-APC , Tonbo , 20–4317 U500 , (1:100-1:400).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Sequencing, Recombinant, Staining, Software

Journal: Journal of neuroscience research

Article Title: The Arousal Effect of Hyperbaric Oxygen through Orexin/hypocretin An Upregulation on Ketamine/ Ethanol-Induced Unconsciousness in Male Rats

doi: 10.1002/jnr.24414

Figure Lengend Snippet: Arousal effects of HBO on the duration of loss of righting reflex (LORR) and Garcia neurological scores in ketamine-induced unconscious rats. (A) HBO significantly shortened the duration of LORR induced by ketamine. (B) HBO significantly improved the acute neurologic deficits in the rats 6 hours after the unconsciousness induction. SB334867 and JNJ10397049 significantly reversed the arousal and neuroprotective effects of HBO by blocking OX1R and OX2R, respectively. n=8/group, *P<0.001 versus Air, # P<0.001 versus HBO.

Article Snippet: SB334867, OX1R inhibitor (10mg/kg, CAS No. 792173-99-0, Sigma-Aldrich, St. Louis, MO, USA) or JNJ10397049, OX2R inhibitor (5mg/kg, Cat No. 4317, Tocris Bioscience, UK) was administered i.p. 30 minutes before unconsciousness induction.

Techniques: Blocking Assay

Journal: Journal of neuroscience research

Article Title: The Arousal Effect of Hyperbaric Oxygen through Orexin/hypocretin An Upregulation on Ketamine/ Ethanol-Induced Unconsciousness in Male Rats

doi: 10.1002/jnr.24414

Figure Lengend Snippet: Arousal effects of HBO on the duration of LORR and Garcia neurological scores in ethanol-induced unconscious rats. (A) HBO significantly shortened the duration of LORR induced by 3.5g/kg ethanol. (B) HBO significantly improved the acute neurologic deficits in the rats 6 hours after the unconsciousness induction. SB334867 and JNJ10397049 significantly reversed the arousal and neuroprotective effects of HBO by blocking OX1R and OX2R, respectively. n=8/group, *P<0.001 versus Air, #P<0.001 versus HBO.

Article Snippet: SB334867, OX1R inhibitor (10mg/kg, CAS No. 792173-99-0, Sigma-Aldrich, St. Louis, MO, USA) or JNJ10397049, OX2R inhibitor (5mg/kg, Cat No. 4317, Tocris Bioscience, UK) was administered i.p. 30 minutes before unconsciousness induction.

Techniques: Blocking Assay

Journal: Journal of neuroscience research

Article Title: The Arousal Effect of Hyperbaric Oxygen through Orexin/hypocretin An Upregulation on Ketamine/ Ethanol-Induced Unconsciousness in Male Rats

doi: 10.1002/jnr.24414

Figure Lengend Snippet: ELISA assay showed that HBO treatment significantly increased the ATP level within the somatosensory cortex and hypothalamus regions 6 hour after unconsciousness induced either by ketamine at 100mg/kg (A) or ethanol at 3.5g/kg (B). Orexin receptors inhibition by SB334867 or JNJ10397049 did not affect HBO-induced upregulation of ATP level. n=6/group, *P<0.001 versus Air.

Article Snippet: SB334867, OX1R inhibitor (10mg/kg, CAS No. 792173-99-0, Sigma-Aldrich, St. Louis, MO, USA) or JNJ10397049, OX2R inhibitor (5mg/kg, Cat No. 4317, Tocris Bioscience, UK) was administered i.p. 30 minutes before unconsciousness induction.

Techniques: Enzyme-linked Immunosorbent Assay, Inhibition

Journal: Journal of neuroscience research

Article Title: The Arousal Effect of Hyperbaric Oxygen through Orexin/hypocretin An Upregulation on Ketamine/ Ethanol-Induced Unconsciousness in Male Rats

doi: 10.1002/jnr.24414

Figure Lengend Snippet: ELISA assay showed that HBO treatment significantly increased the orexin A levels within the somatosensory cortex and hypothalamus regions 6 hour after unconsciousness induced either by ketamine at 100mg/kg (A) or ethanol at 3.5k/kg (B). Orexin receptors inhibition by SB334867 or JNJ10397049 did not affect HBO-induced upregulation of orexin A level. n=6/group, *P<0.001 versus Air.

Article Snippet: SB334867, OX1R inhibitor (10mg/kg, CAS No. 792173-99-0, Sigma-Aldrich, St. Louis, MO, USA) or JNJ10397049, OX2R inhibitor (5mg/kg, Cat No. 4317, Tocris Bioscience, UK) was administered i.p. 30 minutes before unconsciousness induction.

Techniques: Enzyme-linked Immunosorbent Assay, Inhibition