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  • 90
    ATCC imet 42944
    Imet 42944, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imet 42944/product/ATCC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    imet 42944 - by Bioz Stars, 2024-05
    90/100 stars
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    91
    Addgene inc aav1 syn nes jrgeco1a wpre sv40
    (a) Coronal histological section, showing the extent of AAV-mediated expression of GCaMP6f in the mouse medial prefrontal cortex. Cg1, cingulate cortex. M2, secondary motor cortex. PrL, prelimbic cortex. (b) Left and middle, schematic of experimental setup and location. Right, an in vivo two-photon image of GCaMP6f-expressing apical dendritic spines in layer 1 of Cg1/M2 regions of medial prefrontal cortex. Two dendritic spines, s1 and s2, were identified based on their morphology as protrusions attached to the dendrite. Arrows, spines s1 and s2. Dashed lines, the dendritic shaft adjacent to the spines. (c) A scatter plot of the fluorescence transients (ΔF/F) measured from spine s1 against the ΔF/F measured from the adjacent dendritic shaft. Each open circle represents an image frame. Line, a least-squares regression line forced through the origin. (d) Top, example segment of ΔF/F signal recorded from the dendritic shaft (solid green line). Middle, ΔF/F signals recorded from the two dendritic spines, s1 and s2 (solid yellow lines), plotted along with the predicted non-local component of the spine calcium signal – the dendritic shaft calcium signal scaled based on the least-squares regression (broken green lines). Bottom, subtracting the scaled dendritic shaft ΔF/F signal from the dendritic spine ΔF/F signals to get an estimate of the synaptic calcium signals for spines s1 and s2 (solid black lines). (e) In vivo two-photon image of GCaMP6s-expressing long-range axons from retrosplenial cortex and <t>jRGECO1a-expressing</t> dendrites in the medial prefrontal cortex. Note a pair of apposing axonal bouton (arrowhead) and dendritic spine (arrow). (f) Fluorescence traces for the bouton and opposing spine (either with subtraction or no subtraction of dendritic shaft contribution) as denoted by the arrow and arrowhead respectively in (a). (g) Cumulative plot of the conditional probability of detecting a calcium event in an axonal bouton given detection of a calcium event in an apposing dendritic spine (data with subtraction: 0.65 ± 0.01, data without subtraction: 0.329 ± 0.004, shuffled: 0.144 ± 0.004, mean ± s.e.m.; P = 8 × 10 −43 , paired t-test). n = 45 bouton-spine pairs from 3 animals.
    Aav1 Syn Nes Jrgeco1a Wpre Sv40, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aav1 syn nes jrgeco1a wpre sv40/product/Addgene inc
    Average 91 stars, based on 1 article reviews
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    91/100 stars
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    93
    Addgene inc sox2 gene strains pcx oks 2a
    List of the specific sequences of Oct4, Klf4 and <t> Sox2. </t>
    Sox2 Gene Strains Pcx Oks 2a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sox2 gene strains pcx oks 2a/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sox2 gene strains pcx oks 2a - by Bioz Stars, 2024-05
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    Image Search Results


    (a) Coronal histological section, showing the extent of AAV-mediated expression of GCaMP6f in the mouse medial prefrontal cortex. Cg1, cingulate cortex. M2, secondary motor cortex. PrL, prelimbic cortex. (b) Left and middle, schematic of experimental setup and location. Right, an in vivo two-photon image of GCaMP6f-expressing apical dendritic spines in layer 1 of Cg1/M2 regions of medial prefrontal cortex. Two dendritic spines, s1 and s2, were identified based on their morphology as protrusions attached to the dendrite. Arrows, spines s1 and s2. Dashed lines, the dendritic shaft adjacent to the spines. (c) A scatter plot of the fluorescence transients (ΔF/F) measured from spine s1 against the ΔF/F measured from the adjacent dendritic shaft. Each open circle represents an image frame. Line, a least-squares regression line forced through the origin. (d) Top, example segment of ΔF/F signal recorded from the dendritic shaft (solid green line). Middle, ΔF/F signals recorded from the two dendritic spines, s1 and s2 (solid yellow lines), plotted along with the predicted non-local component of the spine calcium signal – the dendritic shaft calcium signal scaled based on the least-squares regression (broken green lines). Bottom, subtracting the scaled dendritic shaft ΔF/F signal from the dendritic spine ΔF/F signals to get an estimate of the synaptic calcium signals for spines s1 and s2 (solid black lines). (e) In vivo two-photon image of GCaMP6s-expressing long-range axons from retrosplenial cortex and jRGECO1a-expressing dendrites in the medial prefrontal cortex. Note a pair of apposing axonal bouton (arrowhead) and dendritic spine (arrow). (f) Fluorescence traces for the bouton and opposing spine (either with subtraction or no subtraction of dendritic shaft contribution) as denoted by the arrow and arrowhead respectively in (a). (g) Cumulative plot of the conditional probability of detecting a calcium event in an axonal bouton given detection of a calcium event in an apposing dendritic spine (data with subtraction: 0.65 ± 0.01, data without subtraction: 0.329 ± 0.004, shuffled: 0.144 ± 0.004, mean ± s.e.m.; P = 8 × 10 −43 , paired t-test). n = 45 bouton-spine pairs from 3 animals.

    Journal: Molecular psychiatry

    Article Title: Inhibitory regulation of calcium transients in prefrontal dendritic spines is compromised by a nonsense Shank3 mutation

    doi: 10.1038/s41380-020-0708-6

    Figure Lengend Snippet: (a) Coronal histological section, showing the extent of AAV-mediated expression of GCaMP6f in the mouse medial prefrontal cortex. Cg1, cingulate cortex. M2, secondary motor cortex. PrL, prelimbic cortex. (b) Left and middle, schematic of experimental setup and location. Right, an in vivo two-photon image of GCaMP6f-expressing apical dendritic spines in layer 1 of Cg1/M2 regions of medial prefrontal cortex. Two dendritic spines, s1 and s2, were identified based on their morphology as protrusions attached to the dendrite. Arrows, spines s1 and s2. Dashed lines, the dendritic shaft adjacent to the spines. (c) A scatter plot of the fluorescence transients (ΔF/F) measured from spine s1 against the ΔF/F measured from the adjacent dendritic shaft. Each open circle represents an image frame. Line, a least-squares regression line forced through the origin. (d) Top, example segment of ΔF/F signal recorded from the dendritic shaft (solid green line). Middle, ΔF/F signals recorded from the two dendritic spines, s1 and s2 (solid yellow lines), plotted along with the predicted non-local component of the spine calcium signal – the dendritic shaft calcium signal scaled based on the least-squares regression (broken green lines). Bottom, subtracting the scaled dendritic shaft ΔF/F signal from the dendritic spine ΔF/F signals to get an estimate of the synaptic calcium signals for spines s1 and s2 (solid black lines). (e) In vivo two-photon image of GCaMP6s-expressing long-range axons from retrosplenial cortex and jRGECO1a-expressing dendrites in the medial prefrontal cortex. Note a pair of apposing axonal bouton (arrowhead) and dendritic spine (arrow). (f) Fluorescence traces for the bouton and opposing spine (either with subtraction or no subtraction of dendritic shaft contribution) as denoted by the arrow and arrowhead respectively in (a). (g) Cumulative plot of the conditional probability of detecting a calcium event in an axonal bouton given detection of a calcium event in an apposing dendritic spine (data with subtraction: 0.65 ± 0.01, data without subtraction: 0.329 ± 0.004, shuffled: 0.144 ± 0.004, mean ± s.e.m.; P = 8 × 10 −43 , paired t-test). n = 45 bouton-spine pairs from 3 animals.

    Article Snippet: For the two-color imaging experiment, two viruses were injected in the same animal: AAV1-Syn-NES-jRGECO1a-WPRE-SV40 (Addgene; 1 × 10 13 GC/mL titer, 294.4 nL) in Cg1/M2, and AAV1-Syn-GCaMP6s-WPRE-SV40 (Penn Vector Core; 3 × 10 13 GC/mL titer, 294.4 nL) in RSC.

    Techniques: Expressing, In Vivo, Fluorescence

    List of the specific sequences of Oct4, Klf4 and  Sox2.

    Journal: PLoS ONE

    Article Title: Non-Genetic Direct Reprogramming and Biomimetic Platforms in a Preliminary Study for Adipose-Derived Stem Cells into Corneal Endothelia-Like Cells

    doi: 10.1371/journal.pone.0109856

    Figure Lengend Snippet: List of the specific sequences of Oct4, Klf4 and Sox2.

    Article Snippet: The plasmids containing the Oct4, Klf4 and Sox2 gene strains pCX-OKS-2A were obtained from Addgene ( http://www.addgene.org ).

    Techniques:

    List of primers.

    Journal: PLoS ONE

    Article Title: Non-Genetic Direct Reprogramming and Biomimetic Platforms in a Preliminary Study for Adipose-Derived Stem Cells into Corneal Endothelia-Like Cells

    doi: 10.1371/journal.pone.0109856

    Figure Lengend Snippet: List of primers.

    Article Snippet: The plasmids containing the Oct4, Klf4 and Sox2 gene strains pCX-OKS-2A were obtained from Addgene ( http://www.addgene.org ).

    Techniques:

    (Up) SDS-PAGE analysis displayed that fusion recombinant proteins of PTD-Oct4/Klf4/Sox2 were loaded onto a Ni affinity column and eluted with 60 mmol/L imidazole (indicated by large arrows). Small arrows revealed the identification of purified reprogramming proteins of PTD-Oct4 (35 kDa), PTD-Klf4 (66.2 bp) and PTD-Sox2 (35.8 kDa) by SDS-PAGE and western blotting (WB). (Down) There were significant FRET signals on 565 nm (PTD-Oct4), 570 nm (PTD-Klf4) and PTD-Sox2 (570 nm) (down A, indicated by arrow heads), while no FRET signal between reprogramming proteins and non-target sequence (down B).

    Journal: PLoS ONE

    Article Title: Non-Genetic Direct Reprogramming and Biomimetic Platforms in a Preliminary Study for Adipose-Derived Stem Cells into Corneal Endothelia-Like Cells

    doi: 10.1371/journal.pone.0109856

    Figure Lengend Snippet: (Up) SDS-PAGE analysis displayed that fusion recombinant proteins of PTD-Oct4/Klf4/Sox2 were loaded onto a Ni affinity column and eluted with 60 mmol/L imidazole (indicated by large arrows). Small arrows revealed the identification of purified reprogramming proteins of PTD-Oct4 (35 kDa), PTD-Klf4 (66.2 bp) and PTD-Sox2 (35.8 kDa) by SDS-PAGE and western blotting (WB). (Down) There were significant FRET signals on 565 nm (PTD-Oct4), 570 nm (PTD-Klf4) and PTD-Sox2 (570 nm) (down A, indicated by arrow heads), while no FRET signal between reprogramming proteins and non-target sequence (down B).

    Article Snippet: The plasmids containing the Oct4, Klf4 and Sox2 gene strains pCX-OKS-2A were obtained from Addgene ( http://www.addgene.org ).

    Techniques: SDS Page, Recombinant, Affinity Column, Purification, Western Blot, Sequencing

    The nuclei of ADSCs (about 50% ∼60%) showed detectable fluorescence from specific antibodies of Oct4, Klf4 and Sox2 after ADSCs were transduced with reprogramming proteins (PTD-Oct4, PTD-Klf4 and PTD-Sox2) respectively for 4 h and then cultivated in conventional medium for 20 h. No fluorescent cells were observed when ADSCs were incubated with PBS in control. Scale bars represent 50 µm and DAPI for nuclear staining.

    Journal: PLoS ONE

    Article Title: Non-Genetic Direct Reprogramming and Biomimetic Platforms in a Preliminary Study for Adipose-Derived Stem Cells into Corneal Endothelia-Like Cells

    doi: 10.1371/journal.pone.0109856

    Figure Lengend Snippet: The nuclei of ADSCs (about 50% ∼60%) showed detectable fluorescence from specific antibodies of Oct4, Klf4 and Sox2 after ADSCs were transduced with reprogramming proteins (PTD-Oct4, PTD-Klf4 and PTD-Sox2) respectively for 4 h and then cultivated in conventional medium for 20 h. No fluorescent cells were observed when ADSCs were incubated with PBS in control. Scale bars represent 50 µm and DAPI for nuclear staining.

    Article Snippet: The plasmids containing the Oct4, Klf4 and Sox2 gene strains pCX-OKS-2A were obtained from Addgene ( http://www.addgene.org ).

    Techniques: Fluorescence, Transduction, Incubation, Staining

    (A) ADSCs formed big and dense aggregations in group E after microgravity culture on day 5. (B) These ADSCs spheroids readily attached to the surface of plates after they were re-plated onto the adherent culture plates on day 5. (C) The spheroids generated cells that eventually repopulated as a confluent monolayer on day 7. These adherent ADSCs spheroids in group E positively expressed Nanog (D), while negatively expressed Oct4 (E), Sox2 (F) and Klf4 (G) by immunofluorescence staining on day 9. ADSCs spheroids in group D (H) and cells in control group (I) did not express Nanog by immunofluorescence staining. Scale bars represent 100 µm and DAPI for nuclear staining (D–I).

    Journal: PLoS ONE

    Article Title: Non-Genetic Direct Reprogramming and Biomimetic Platforms in a Preliminary Study for Adipose-Derived Stem Cells into Corneal Endothelia-Like Cells

    doi: 10.1371/journal.pone.0109856

    Figure Lengend Snippet: (A) ADSCs formed big and dense aggregations in group E after microgravity culture on day 5. (B) These ADSCs spheroids readily attached to the surface of plates after they were re-plated onto the adherent culture plates on day 5. (C) The spheroids generated cells that eventually repopulated as a confluent monolayer on day 7. These adherent ADSCs spheroids in group E positively expressed Nanog (D), while negatively expressed Oct4 (E), Sox2 (F) and Klf4 (G) by immunofluorescence staining on day 9. ADSCs spheroids in group D (H) and cells in control group (I) did not express Nanog by immunofluorescence staining. Scale bars represent 100 µm and DAPI for nuclear staining (D–I).

    Article Snippet: The plasmids containing the Oct4, Klf4 and Sox2 gene strains pCX-OKS-2A were obtained from Addgene ( http://www.addgene.org ).

    Techniques: Generated, Immunofluorescence, Staining

    The gene expressions of Nanog of human ADSCs spheroids after 7 cycle treatment of PTD-OKS and purmorphamine in group D and after microgravity culture in group E was positively displayed. However, ADSCs in control group did not express Nanog gene. The undifferentiated gene expressions of Oct4, Sox2, Klf4 were negative in all ADSCs and GAPDH were expressed in all ADSCs.

    Journal: PLoS ONE

    Article Title: Non-Genetic Direct Reprogramming and Biomimetic Platforms in a Preliminary Study for Adipose-Derived Stem Cells into Corneal Endothelia-Like Cells

    doi: 10.1371/journal.pone.0109856

    Figure Lengend Snippet: The gene expressions of Nanog of human ADSCs spheroids after 7 cycle treatment of PTD-OKS and purmorphamine in group D and after microgravity culture in group E was positively displayed. However, ADSCs in control group did not express Nanog gene. The undifferentiated gene expressions of Oct4, Sox2, Klf4 were negative in all ADSCs and GAPDH were expressed in all ADSCs.

    Article Snippet: The plasmids containing the Oct4, Klf4 and Sox2 gene strains pCX-OKS-2A were obtained from Addgene ( http://www.addgene.org ).

    Techniques:

    (A) Human ADSCs on decellularized corneas after sequential non-genetic direct reprogramming with co-culture treatments of both of R-CECs and R-CSCs were obviously positive staining for vimentin and weakly expressed CD31, AQP-1 and ZO-1. (B) ADSCs on decellularized corneas after sequential non-genetic direct reprogramming without co-culture treatments were positive staining for vimentin but negative for CD31, AQP-1 and ZO-1. (C) The undifferentiated gene transcripts of Oct4, Sox2, Klf4 and Nanog could not be detected in ADSCs on decellularized corneas after sequential non-genetic direct reprogramming with (a) or without (b) co-culture treatments. Scale bars represent 100 µm and DAPI for nuclear staining (A, B).

    Journal: PLoS ONE

    Article Title: Non-Genetic Direct Reprogramming and Biomimetic Platforms in a Preliminary Study for Adipose-Derived Stem Cells into Corneal Endothelia-Like Cells

    doi: 10.1371/journal.pone.0109856

    Figure Lengend Snippet: (A) Human ADSCs on decellularized corneas after sequential non-genetic direct reprogramming with co-culture treatments of both of R-CECs and R-CSCs were obviously positive staining for vimentin and weakly expressed CD31, AQP-1 and ZO-1. (B) ADSCs on decellularized corneas after sequential non-genetic direct reprogramming without co-culture treatments were positive staining for vimentin but negative for CD31, AQP-1 and ZO-1. (C) The undifferentiated gene transcripts of Oct4, Sox2, Klf4 and Nanog could not be detected in ADSCs on decellularized corneas after sequential non-genetic direct reprogramming with (a) or without (b) co-culture treatments. Scale bars represent 100 µm and DAPI for nuclear staining (A, B).

    Article Snippet: The plasmids containing the Oct4, Klf4 and Sox2 gene strains pCX-OKS-2A were obtained from Addgene ( http://www.addgene.org ).

    Techniques: Co-Culture Assay, Staining