Journal: Oncogenesis

Article Title: Post-transcriptional screen of cancer amplified genes identifies ERBB2 / Her2 signaling as AU-rich mRNA stability-promoting pathway

doi: 10.1038/s41389-021-00351-w

Figure Lengend Snippet: A Immunoblot analysis of ERBB2, AKT, MEK1/2, and ERK1/2 signaling inhibition after treatment with increasing doses of lapatinib for 24 h. Status of ERBB2 (phospho-tyrosine 1221/1222 and total), AKT (phospho-serine 473 and total pan AKT), ERK1/2 (Phospho-threonine 202/phospho-tyrosine 204 and total), MEK1/2 (Ser217/221), and ZFP36/TTP are shown. A blot from at least two experiments is shown. B Immunoblot analysis for the inhibition of MK2 activation after treatment with lapatinib. SKBR3 cells were treated with increasing doses of lapatinib, as indicated for 24 h. Phospho-threonine MK2 (T222) and total MK2 protein abundance are shown. A representative blot from two experiments is shown. C Immunoblot analysis for ZFP36/TTP phosphorylation in MAPKAPK-2 silenced SKBR3 cells. SKBR3 cells transfected with 100 nM of si Ctrl or si MAPKAPK-2 for 24 h were tested for ZFP36/TTP protein abundance. Total MK2, phosphorylated ZFP36/TTP, and β-actin protein abundances are shown. A representative blot from three experiments is shown. Effect of the MK2 inhibitor PF-3644022 (1 µM, 4 h) on the abundance of phosphorylated ZFP36/TTP in ERBB2-expressing SKBR3 ( D ) or SKBR3 transfected with ZFP36 expression plasmid ( E ). Western blots are from two independent experiments. F Immunoblot analysis of total and phosphorylated ZFP36/TTP, and MK2 in MK2 -knockout MEF cells in the absence or presence of overnight transfected ERBB2.

Article Snippet: PF-3644022 (MK2 inhibitor) was purchased from Tocris (Tocris Bioscience, Minneapolis, MN).

Techniques: Western Blot, Inhibition, Activation Assay, Transfection, Expressing, Plasmid Preparation, Knock-Out

Journal:

Article Title: Unconventional Genomic Organization in the Alpha Subgroup of the Proteobacteria

doi:

Figure Lengend Snippet: Strain designations, number and sizes of replicons, and estimated genome sizes for organisms belonging to the family Rhizobiaceae

Article Snippet: Strain designations, number and sizes of replicons, and estimated genome sizes for organisms belonging to the family Rhizobiaceae table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Bacterium Strain No. of replicons a Sizes (kb) of replicons b Genome size estimate (kb) Reference M. dimorpha ATCC 4279 T 3 (NE) 3,200, 300, 150 3,150 This study Rhodobacter capsulatus ATCC 11166 2 (1) 3,800*, 150 3,950 This study SB1003 2 (1) 3,800*, 134 3,934 12 Rhodobacter spheroides 2.4.1 7 (2) 3,000*, 900*; 5 plasmids 4,350 39 Brucella melitensis 16M 2 (2) 2,050*, 1,150* 3,200 27 O. anthropi ATCC 49188 T 4 (2) 2,700*, 1,900*, 150, 100 4,850 This study LMG 3301 4 (NE) 2,700, 1,900, 50, <50 4,700 This study Bartonella quintana VR960 1 1,700 1,700 32 Open in a separate window a The numbers in parentheses are the numbers of replicons labeled with the 16S rRNA probe; NE, not examined. b Asterisks indicate replicons labeled by the 16S rRNA probe.

Techniques:

Journal:

Article Title: Unconventional Genomic Organization in the Alpha Subgroup of the Proteobacteria

doi:

Figure Lengend Snippet: Strain designation, number and sizes of replicons, and estimated genome sizes for organisms related to the family Rhizobiaceae

Article Snippet: Strain designations, number and sizes of replicons, and estimated genome sizes for organisms belonging to the family Rhizobiaceae table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Bacterium Strain No. of replicons a Sizes (kb) of replicons b Genome size estimate (kb) Reference M. dimorpha ATCC 4279 T 3 (NE) 3,200, 300, 150 3,150 This study Rhodobacter capsulatus ATCC 11166 2 (1) 3,800*, 150 3,950 This study SB1003 2 (1) 3,800*, 134 3,934 12 Rhodobacter spheroides 2.4.1 7 (2) 3,000*, 900*; 5 plasmids 4,350 39 Brucella melitensis 16M 2 (2) 2,050*, 1,150* 3,200 27 O. anthropi ATCC 49188 T 4 (2) 2,700*, 1,900*, 150, 100 4,850 This study LMG 3301 4 (NE) 2,700, 1,900, 50, <50 4,700 This study Bartonella quintana VR960 1 1,700 1,700 32 Open in a separate window a The numbers in parentheses are the numbers of replicons labeled with the 16S rRNA probe; NE, not examined. b Asterisks indicate replicons labeled by the 16S rRNA probe.

Techniques: