42464 Search Results


42464 strain  (ATCC)
96
ATCC 42464 strain
ku70 disruption of wild-type M. <t>thermophila</t> <t>ATCC</t> <t>42464.</t> a Pattern of the ku70 knockout cassette integrating into the chromosomes of M. thermophila ATCC 42464 via ectopic insertion or homologous recombination. b PCR analysis of ku70 deletion transformants with one primer (ku70KO-F) located in the bar gene cassette and the other (ku70KO-R) located in the downstream of 3′ flank in the genomic DNA (expected product of 1872 bp) and c Southern blotting analysis of ku70 mutants with Xho I digested genomic DNA and the probe amplified from the 3′ flank. Lines 1–11 in ( b ) and ( c ), genomic DNA from ku70 mutants (expected product of 5056 bp). WT, genomic DNA from wild-type as negative control (expected product of 1703 bp). Primers are represented by solid black arrows. Abbreviations: gDNA, genomic DNA; RB and LB, right and left border of T-DNA, respectively
42464 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ebf1a plasmid 42464  (Addgene inc)
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ku70 disruption of wild-type M. thermophila ATCC 42464. a Pattern of the ku70 knockout cassette integrating into the chromosomes of M. thermophila ATCC 42464 via ectopic insertion or homologous recombination. b PCR analysis of ku70 deletion transformants with one primer (ku70KO-F) located in the bar gene cassette and the other (ku70KO-R) located in the downstream of 3′ flank in the genomic DNA (expected product of 1872 bp) and c Southern blotting analysis of ku70 mutants with Xho I digested genomic DNA and the probe amplified from the 3′ flank. Lines 1–11 in ( b ) and ( c ), genomic DNA from ku70 mutants (expected product of 5056 bp). WT, genomic DNA from wild-type as negative control (expected product of 1703 bp). Primers are represented by solid black arrows. Abbreviations: gDNA, genomic DNA; RB and LB, right and left border of T-DNA, respectively

Journal: BMC Biotechnology

Article Title: Development of genetic tools for Myceliophthora thermophila

doi: 10.1186/s12896-015-0165-5

Figure Lengend Snippet: ku70 disruption of wild-type M. thermophila ATCC 42464. a Pattern of the ku70 knockout cassette integrating into the chromosomes of M. thermophila ATCC 42464 via ectopic insertion or homologous recombination. b PCR analysis of ku70 deletion transformants with one primer (ku70KO-F) located in the bar gene cassette and the other (ku70KO-R) located in the downstream of 3′ flank in the genomic DNA (expected product of 1872 bp) and c Southern blotting analysis of ku70 mutants with Xho I digested genomic DNA and the probe amplified from the 3′ flank. Lines 1–11 in ( b ) and ( c ), genomic DNA from ku70 mutants (expected product of 5056 bp). WT, genomic DNA from wild-type as negative control (expected product of 1703 bp). Primers are represented by solid black arrows. Abbreviations: gDNA, genomic DNA; RB and LB, right and left border of T-DNA, respectively

Article Snippet: The C1 strain is a patented strain, therefore the ATCC 42464 strain is predominantly used for public research as the general wild-type strain of M. thermophila .

Techniques: Knock-Out, Homologous Recombination, Southern Blot, Amplification, Negative Control

pyrG gene deletion in wild type and ∆ku70 mutant of M. thermophila ATCC 42464. The experiment design ( a ), PCR analysis of transformants under wild-type background ( b ) and ∆ku70 mutant ( c ) with one primer (pyrGout-F) located in the neo gene cassette and the other (pyrGout-R) located in the downstream of 3′ flank in the genomic DNA. The genomic DNA from the wild-type (WT) and ∆ku70 strains was used as the negative control

Journal: BMC Biotechnology

Article Title: Development of genetic tools for Myceliophthora thermophila

doi: 10.1186/s12896-015-0165-5

Figure Lengend Snippet: pyrG gene deletion in wild type and ∆ku70 mutant of M. thermophila ATCC 42464. The experiment design ( a ), PCR analysis of transformants under wild-type background ( b ) and ∆ku70 mutant ( c ) with one primer (pyrGout-F) located in the neo gene cassette and the other (pyrGout-R) located in the downstream of 3′ flank in the genomic DNA. The genomic DNA from the wild-type (WT) and ∆ku70 strains was used as the negative control

Article Snippet: The C1 strain is a patented strain, therefore the ATCC 42464 strain is predominantly used for public research as the general wild-type strain of M. thermophila .

Techniques: Mutagenesis, Negative Control