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  • 9 3f10  (ATCC)
    90
    ATCC 9 3f10
    9 3f10, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/9 3f10/product/ATCC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    9 3f10 - by Bioz Stars, 2024-04
    90/100 stars
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    93
    DSMZ streptomyces sp
    Streptomyces Sp, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptomyces sp/product/DSMZ
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptomyces sp - by Bioz Stars, 2024-04
    93/100 stars
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    92
    Santa Cruz Biotechnology cmv promoter
    Silencing netrin-1 by construction of a siRNA plasmid. (A) Illustration of plasmids carrying the siRNA for netrin-1 or a scrambled netrin-1 sequence and a luciferase reporter under a <t>CMV</t> <t>promoter;</t> (B) relative netrin-1 mRNA expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by RT-qPCR; (C) relative netrin-1 protein expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by ELISA. Rb cell lines: Y79, WERI-Rb-1, NCC-Rbc-57, NCC-Rbc-92, and NCC-Rbc-T1; *, P<0.05; N=5. IRES, internal ribosome entry site; CMV, cytomegalovirus; RT-qPCR, 1uantitative reverse transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.
    Cmv Promoter, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cmv promoter/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cmv promoter - by Bioz Stars, 2024-04
    92/100 stars
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    92
    Addgene inc pcdna5 frt to sbp tnrc6a
    Silencing netrin-1 by construction of a siRNA plasmid. (A) Illustration of plasmids carrying the siRNA for netrin-1 or a scrambled netrin-1 sequence and a luciferase reporter under a <t>CMV</t> <t>promoter;</t> (B) relative netrin-1 mRNA expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by RT-qPCR; (C) relative netrin-1 protein expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by ELISA. Rb cell lines: Y79, WERI-Rb-1, NCC-Rbc-57, NCC-Rbc-92, and NCC-Rbc-T1; *, P<0.05; N=5. IRES, internal ribosome entry site; CMV, cytomegalovirus; RT-qPCR, 1uantitative reverse transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.
    Pcdna5 Frt To Sbp Tnrc6a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna5 frt to sbp tnrc6a/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcdna5 frt to sbp tnrc6a - by Bioz Stars, 2024-04
    92/100 stars
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    86
    Santa Cruz Biotechnology netrin 1 shrna plasmid
    Silencing netrin-1 by construction of a siRNA plasmid. (A) Illustration of plasmids carrying the siRNA for netrin-1 or a scrambled netrin-1 sequence and a luciferase reporter under a <t>CMV</t> <t>promoter;</t> (B) relative netrin-1 mRNA expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by RT-qPCR; (C) relative netrin-1 protein expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by ELISA. Rb cell lines: Y79, WERI-Rb-1, NCC-Rbc-57, NCC-Rbc-92, and NCC-Rbc-T1; *, P<0.05; N=5. IRES, internal ribosome entry site; CMV, cytomegalovirus; RT-qPCR, 1uantitative reverse transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.
    Netrin 1 Shrna Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/netrin 1 shrna plasmid/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    netrin 1 shrna plasmid - by Bioz Stars, 2024-04
    86/100 stars
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    Image Search Results


    Silencing netrin-1 by construction of a siRNA plasmid. (A) Illustration of plasmids carrying the siRNA for netrin-1 or a scrambled netrin-1 sequence and a luciferase reporter under a CMV promoter; (B) relative netrin-1 mRNA expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by RT-qPCR; (C) relative netrin-1 protein expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by ELISA. Rb cell lines: Y79, WERI-Rb-1, NCC-Rbc-57, NCC-Rbc-92, and NCC-Rbc-T1; *, P<0.05; N=5. IRES, internal ribosome entry site; CMV, cytomegalovirus; RT-qPCR, 1uantitative reverse transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.

    Journal: Annals of Translational Medicine

    Article Title: Netrin-1 promotes retinoblastoma-associated angiogenesis

    doi: 10.21037/atm-21-5560

    Figure Lengend Snippet: Silencing netrin-1 by construction of a siRNA plasmid. (A) Illustration of plasmids carrying the siRNA for netrin-1 or a scrambled netrin-1 sequence and a luciferase reporter under a CMV promoter; (B) relative netrin-1 mRNA expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by RT-qPCR; (C) relative netrin-1 protein expression in Rb cells transfected with si-netrin-1 or scrambled plasmid as assessed by ELISA. Rb cell lines: Y79, WERI-Rb-1, NCC-Rbc-57, NCC-Rbc-92, and NCC-Rbc-T1; *, P<0.05; N=5. IRES, internal ribosome entry site; CMV, cytomegalovirus; RT-qPCR, 1uantitative reverse transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.

    Article Snippet: Plasmids expressing small interfering (si)RNA netrin-1 or a scrambled sequence for netrin-1 together with a luciferase reporter (to allow in vivo cell tracing by bioluminescent assay) under a CMV promoter were purchased from Santa Cruz (SC-42044, Beijing, China).

    Techniques: Plasmid Preparation, Sequencing, Luciferase, Expressing, Transfection, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction