Journal: Cell Death Discovery

Article Title: Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells

doi: 10.1038/s41420-020-00336-5

Figure Lengend Snippet: a Comparison of SEMG1 and SEMG2. PSA – prostate specific antigen, cleaves SEMG1 and SEMG2 to small peptides indicated. b Distribution of SEMG1 and SEMG2 in different NSCLC cell lines. Western-blot analysis.

Article Snippet: For western-blotting following antibodies were used: anti-SEMG1 (1:1000, PA5-30168, Invitrogen), anti-SEMG2 (1:1000, PA5-42099, Invitrogen), anti-Flag (1:1000, M2, Sigma, USA) and anti-β-actin (1:1000, A-2228, Sigma, USA).

Techniques: Western Blot

Journal: Cell Death Discovery

Article Title: Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells

doi: 10.1038/s41420-020-00336-5

Figure Lengend Snippet: a H520, b H1299 and c H1650 cells. Secondary antibodies conjugated with Alexa546 and Alexa 488 were used to visualize primary anti-SEMG1 and anti-SEMG2 antibodies.

Article Snippet: For western-blotting following antibodies were used: anti-SEMG1 (1:1000, PA5-30168, Invitrogen), anti-SEMG2 (1:1000, PA5-42099, Invitrogen), anti-Flag (1:1000, M2, Sigma, USA) and anti-β-actin (1:1000, A-2228, Sigma, USA).

Techniques:

Journal: Cell Death Discovery

Article Title: Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells

doi: 10.1038/s41420-020-00336-5

Figure Lengend Snippet: a H1299 cells stably overexpressed SEMG1, SEMG2 or vehicle (western-blot). b Proliferation assay based on cell counting. Results are represented as mean ± SEM of five experiments. * P < 0.05; ** P < 0.01; c – e Cell cycle analysis of H1299 cells overexpressing SEMG1, SEMG2 or vehicle. Results are represented as mean ± SEM of three experiments. * P < 0.05; ** P < 0.01.

Article Snippet: For western-blotting following antibodies were used: anti-SEMG1 (1:1000, PA5-30168, Invitrogen), anti-SEMG2 (1:1000, PA5-42099, Invitrogen), anti-Flag (1:1000, M2, Sigma, USA) and anti-β-actin (1:1000, A-2228, Sigma, USA).

Techniques: Stable Transfection, Western Blot, Proliferation Assay, Cell Counting, Cell Cycle Assay

Journal: Cell Death Discovery

Article Title: Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells

doi: 10.1038/s41420-020-00336-5

Figure Lengend Snippet: MTT assay of H1299 cells overexpressing vector, SEMG1, SEMG2 and incubated with different concentrations of a doxorubicin or b cisplatin. c MTT assay of H1299 cells with scramble or SEMG2 knockdown (KD) treated with cisplatin. Knockdown of SEMG2 is visualized by western-blotting. d Apoptosis profile of non-treated or treated with 30 μM of cisplatin or 75 μM of etoposide H1299 cells overexpressing vector, SEMG1 or SEMG2. e Percentage of apoptosis increase in H1299 cells with vector, SEMG1 or SEMG2 overexpressing after treatment with etoposide or cisplatin. Results are represented as mean ± SEM of three experiments. ** P < 0.01.

Article Snippet: For western-blotting following antibodies were used: anti-SEMG1 (1:1000, PA5-30168, Invitrogen), anti-SEMG2 (1:1000, PA5-42099, Invitrogen), anti-Flag (1:1000, M2, Sigma, USA) and anti-β-actin (1:1000, A-2228, Sigma, USA).

Techniques: MTT Assay, Plasmid Preparation, Incubation, Western Blot