Journal: BMC Microbiology

Article Title: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

doi: 10.1186/1471-2180-13-82

Figure Lengend Snippet: Bacterial strains used in PNA-FISH assays and their specificity with Lac663 and Gard162 probes

Article Snippet: L. acidophilus; , ATCC 4356 T ; CCUG 42099 T , ++++ , -.

Techniques:

Journal: BMC Microbiology

Article Title: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

doi: 10.1186/1471-2180-13-82

Figure Lengend Snippet: Results of the Lac663 and Gard162 probes specificity test in artificial mixed samples

Article Snippet: L. acidophilus; , ATCC 4356 T ; CCUG 42099 T , ++++ , -.

Techniques: Multiplex Assay

Journal: Frontiers in Neuroscience

Article Title: Upregulation of neuropeptide Y in cardiac sympathetic nerves induces stress (Takotsubo) cardiomyopathy

doi: 10.3389/fnins.2022.1013712

Figure Lengend Snippet: Upregulation of neuropeptide Y (NPY) expression in the left stellate ganglion (SG) and cardiac sympathetic nerves. (A) mRNA expression of various neuronal markers in the left SG in the control (CTL) and stress cardiomyopathy (SC) groups. SGs in SC model were collected 2 h after the commencement of epilepsy. NPY expression was significantly increased in the SC model, whereas there was no increase in the expression of the choline transporter (CHT), choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAchT), or tyrosine hydroxylase (TH; n = 5). (B,C) Triple-immunohistochemistry staining of the left SG and left ventricle (LV) for TH (green), NPY (red), and 4′,6′-diamidino-2-phenylindole (DAPI; nuclei; blue). NPY upregulation was observed in the SG and cardiac sympathetic nerve of the LV in the SC model. Scale bars: 50 μm (B) and 100 μm (C) . (D) NPY content of the apical part of the LV in control and SC rats ( n = 6). (E) Expression level of NPY mRNA significantly reduced following the injection of NPY siRNA into left SG compared to control siRNA ( n = 6). Where appropriate, data are provided as the mean ± SD. * P < 0.05 relative to the control.

Article Snippet: Thereafter (mechanically ventilated), their left stellate ganglia (SG) were microinjected with NPY siRNA (100 ng/0.5 μl; Santa Cruz Biochemical, Santa Cruz, CA, USA) using a Hamilton micropipette.

Techniques: Expressing, Immunohistochemistry, Staining, Injection

Journal: Cell Death Discovery

Article Title: Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells

doi: 10.1038/s41420-020-00336-5

Figure Lengend Snippet: a Comparison of SEMG1 and SEMG2. PSA – prostate specific antigen, cleaves SEMG1 and SEMG2 to small peptides indicated. b Distribution of SEMG1 and SEMG2 in different NSCLC cell lines. Western-blot analysis.

Article Snippet: For western-blotting following antibodies were used: anti-SEMG1 (1:1000, PA5-30168, Invitrogen), anti-SEMG2 (1:1000, PA5-42099, Invitrogen), anti-Flag (1:1000, M2, Sigma, USA) and anti-β-actin (1:1000, A-2228, Sigma, USA).

Techniques: Western Blot

Journal: Cell Death Discovery

Article Title: Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells

doi: 10.1038/s41420-020-00336-5

Figure Lengend Snippet: a H520, b H1299 and c H1650 cells. Secondary antibodies conjugated with Alexa546 and Alexa 488 were used to visualize primary anti-SEMG1 and anti-SEMG2 antibodies.

Article Snippet: For western-blotting following antibodies were used: anti-SEMG1 (1:1000, PA5-30168, Invitrogen), anti-SEMG2 (1:1000, PA5-42099, Invitrogen), anti-Flag (1:1000, M2, Sigma, USA) and anti-β-actin (1:1000, A-2228, Sigma, USA).

Techniques:

Journal: Cell Death Discovery

Article Title: Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells

doi: 10.1038/s41420-020-00336-5

Figure Lengend Snippet: a H1299 cells stably overexpressed SEMG1, SEMG2 or vehicle (western-blot). b Proliferation assay based on cell counting. Results are represented as mean ± SEM of five experiments. * P < 0.05; ** P < 0.01; c – e Cell cycle analysis of H1299 cells overexpressing SEMG1, SEMG2 or vehicle. Results are represented as mean ± SEM of three experiments. * P < 0.05; ** P < 0.01.

Article Snippet: For western-blotting following antibodies were used: anti-SEMG1 (1:1000, PA5-30168, Invitrogen), anti-SEMG2 (1:1000, PA5-42099, Invitrogen), anti-Flag (1:1000, M2, Sigma, USA) and anti-β-actin (1:1000, A-2228, Sigma, USA).

Techniques: Stable Transfection, Western Blot, Proliferation Assay, Cell Counting, Cell Cycle Assay

Journal: Cell Death Discovery

Article Title: Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells

doi: 10.1038/s41420-020-00336-5

Figure Lengend Snippet: MTT assay of H1299 cells overexpressing vector, SEMG1, SEMG2 and incubated with different concentrations of a doxorubicin or b cisplatin. c MTT assay of H1299 cells with scramble or SEMG2 knockdown (KD) treated with cisplatin. Knockdown of SEMG2 is visualized by western-blotting. d Apoptosis profile of non-treated or treated with 30 μM of cisplatin or 75 μM of etoposide H1299 cells overexpressing vector, SEMG1 or SEMG2. e Percentage of apoptosis increase in H1299 cells with vector, SEMG1 or SEMG2 overexpressing after treatment with etoposide or cisplatin. Results are represented as mean ± SEM of three experiments. ** P < 0.01.

Article Snippet: For western-blotting following antibodies were used: anti-SEMG1 (1:1000, PA5-30168, Invitrogen), anti-SEMG2 (1:1000, PA5-42099, Invitrogen), anti-Flag (1:1000, M2, Sigma, USA) and anti-β-actin (1:1000, A-2228, Sigma, USA).

Techniques: MTT Assay, Plasmid Preparation, Incubation, Western Blot