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  • 95
    Santa Cruz Biotechnology vegfr2
    Gene knockdown of VEGFR- 2,  NP1 and NP2 attenuates VEGF- mediated cell survival.  VEGFR siRNA was carried out in A549  (A)  and SKMES1  (B)  lung cancer cells over 24, 48 and 72 h and protein expression was examined by Western blot analysis. NSCLC cells were transfected with siNP1 (100 nM), siNP2 (100 nM), anti-KDR (10 μg/ml) antibody alone, and in combination, for 48 h. While an IgG isotype antibody was used as a control for antibody specificity for KDR, proliferation in response to each siRNA was measured relative to a scrambled control siRNA for siNP1 and siNP2. Exogenous recombinant VEGF (100 ng/ml) was added for a further 24 h following receptor knockdown/blockade, after which time, cell proliferation was measured  (C)  ( A549 cells , *p 
    Vegfr2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegfr2/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegfr2 - by Bioz Stars, 2021-05
    95/100 stars
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    93
    Cell Signaling Technology Inc akt3
    Protein levels of the different Akt isoenzymes. Primary brown adipocytes were lysed and lysates were immunoblotted for Akt1, Akt2, <t>Akt3</t> and tubulin. The blot was developed using the Licor Odyssey system (Lincoln, NE, USA).
    Akt3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/akt3/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    akt3 - by Bioz Stars, 2021-05
    93/100 stars
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    99
    Sino Biological sars cov 2 2019 ncov spike s1 his recombinant protein hplc verified covid 19 spike s1 research
    Protein levels of the different Akt isoenzymes. Primary brown adipocytes were lysed and lysates were immunoblotted for Akt1, Akt2, <t>Akt3</t> and tubulin. The blot was developed using the Licor Odyssey system (Lincoln, NE, USA).
    Sars Cov 2 2019 Ncov Spike S1 His Recombinant Protein Hplc Verified Covid 19 Spike S1 Research, supplied by Sino Biological, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 2019 ncov spike s1 his recombinant protein hplc verified covid 19 spike s1 research/product/Sino Biological
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 2019 ncov spike s1 his recombinant protein hplc verified covid 19 spike s1 research - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

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    Gene knockdown of VEGFR- 2,  NP1 and NP2 attenuates VEGF- mediated cell survival.  VEGFR siRNA was carried out in A549  (A)  and SKMES1  (B)  lung cancer cells over 24, 48 and 72 h and protein expression was examined by Western blot analysis. NSCLC cells were transfected with siNP1 (100 nM), siNP2 (100 nM), anti-KDR (10 μg/ml) antibody alone, and in combination, for 48 h. While an IgG isotype antibody was used as a control for antibody specificity for KDR, proliferation in response to each siRNA was measured relative to a scrambled control siRNA for siNP1 and siNP2. Exogenous recombinant VEGF (100 ng/ml) was added for a further 24 h following receptor knockdown/blockade, after which time, cell proliferation was measured  (C)  ( A549 cells , *p 

    Journal: Molecular Cancer

    Article Title: Vascular endothelial growth factor is an autocrine growth factor, signaling through neuropilin-1 in non-small cell lung cancer

    doi: 10.1186/s12943-015-0310-8

    Figure Lengend Snippet: Gene knockdown of VEGFR- 2, NP1 and NP2 attenuates VEGF- mediated cell survival. VEGFR siRNA was carried out in A549 (A) and SKMES1 (B) lung cancer cells over 24, 48 and 72 h and protein expression was examined by Western blot analysis. NSCLC cells were transfected with siNP1 (100 nM), siNP2 (100 nM), anti-KDR (10 μg/ml) antibody alone, and in combination, for 48 h. While an IgG isotype antibody was used as a control for antibody specificity for KDR, proliferation in response to each siRNA was measured relative to a scrambled control siRNA for siNP1 and siNP2. Exogenous recombinant VEGF (100 ng/ml) was added for a further 24 h following receptor knockdown/blockade, after which time, cell proliferation was measured (C) ( A549 cells , *p 

    Article Snippet: As an alternative to siRNA, due to low levels of knockdown of VEGFR-2, a blocking antibody to VEGFR-2 (sc-19530) (Santa Cruz Biotech, Germany) was also used.

    Techniques: Expressing, Western Blot, Transfection, Recombinant

    VEGF - mediated survival of NSCLC cells. Lung tumor cells were examined for their expression of VEGF mRNA and protein (A) . The VEGF receptors, VEGFR-1, VEGFR-2, NP1 and NP2 were also assessed at the mRNA (B) and protein (C) levels by RT-PCR and Western blot analysis, respectively. All four NSCLC cell lines were treated with recombinant human VEGF (100 ng/ml) for 48 h. Cell proliferation was then measured using the BrdU cell proliferation ELISA assay (*p

    Journal: Molecular Cancer

    Article Title: Vascular endothelial growth factor is an autocrine growth factor, signaling through neuropilin-1 in non-small cell lung cancer

    doi: 10.1186/s12943-015-0310-8

    Figure Lengend Snippet: VEGF - mediated survival of NSCLC cells. Lung tumor cells were examined for their expression of VEGF mRNA and protein (A) . The VEGF receptors, VEGFR-1, VEGFR-2, NP1 and NP2 were also assessed at the mRNA (B) and protein (C) levels by RT-PCR and Western blot analysis, respectively. All four NSCLC cell lines were treated with recombinant human VEGF (100 ng/ml) for 48 h. Cell proliferation was then measured using the BrdU cell proliferation ELISA assay (*p

    Article Snippet: As an alternative to siRNA, due to low levels of knockdown of VEGFR-2, a blocking antibody to VEGFR-2 (sc-19530) (Santa Cruz Biotech, Germany) was also used.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay

    Protein levels of the different Akt isoenzymes. Primary brown adipocytes were lysed and lysates were immunoblotted for Akt1, Akt2, Akt3 and tubulin. The blot was developed using the Licor Odyssey system (Lincoln, NE, USA).

    Journal: Diabetologia

    Article Title: The role of mouse Akt2 in insulin-dependent suppression of adipocyte lipolysis in vivo

    doi: 10.1007/s00125-015-3532-9

    Figure Lengend Snippet: Protein levels of the different Akt isoenzymes. Primary brown adipocytes were lysed and lysates were immunoblotted for Akt1, Akt2, Akt3 and tubulin. The blot was developed using the Licor Odyssey system (Lincoln, NE, USA).

    Article Snippet: Antibodies directed towards Akt1, Akt2 and Akt3 were obtained from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: