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    Sino Biological sars cov 2 2019 ncov spike rbd his recombinant protein covid 19 spike rbd research
    Serum concentration vs. time profiles of CA521 FALA in mice and rhesus monkeys. a Four mice were administered intravenously at a dose of 10 mg/kg with CA521 FALA . Antibody concentrations in serum were determined in Elisa with <t>SARS-CoV-2</t> (2019-nCoV) spike protein as the capture reagent. b Three healthy rhesus monkeys were administered intravenously at a dose of 50 mg/kg with CA521 FALA . The antibody concentration in serum at different time points were determined in Elisa with SARS-CoV-2 (2019-nCoV) spike protein as the capture reagent. The main PK kinetic parameters were calculated using Phoenix WinNonlin.
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    Sino Biological pbs
    Serum concentration vs. time profiles of CA521 FALA in mice and rhesus monkeys. a Four mice were administered intravenously at a dose of 10 mg/kg with CA521 FALA . Antibody concentrations in serum were determined in Elisa with <t>SARS-CoV-2</t> (2019-nCoV) spike protein as the capture reagent. b Three healthy rhesus monkeys were administered intravenously at a dose of 50 mg/kg with CA521 FALA . The antibody concentration in serum at different time points were determined in Elisa with SARS-CoV-2 (2019-nCoV) spike protein as the capture reagent. The main PK kinetic parameters were calculated using Phoenix WinNonlin.
    Pbs, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-06
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    Serum concentration vs. time profiles of CA521 FALA in mice and rhesus monkeys. a Four mice were administered intravenously at a dose of 10 mg/kg with CA521 FALA . Antibody concentrations in serum were determined in Elisa with SARS-CoV-2 (2019-nCoV) spike protein as the capture reagent. b Three healthy rhesus monkeys were administered intravenously at a dose of 50 mg/kg with CA521 FALA . The antibody concentration in serum at different time points were determined in Elisa with SARS-CoV-2 (2019-nCoV) spike protein as the capture reagent. The main PK kinetic parameters were calculated using Phoenix WinNonlin.

    Journal: Communications Biology

    Article Title: Structure and function analysis of a potent human neutralizing antibody CA521FALA against SARS-CoV-2

    doi: 10.1038/s42003-021-02029-w

    Figure Lengend Snippet: Serum concentration vs. time profiles of CA521 FALA in mice and rhesus monkeys. a Four mice were administered intravenously at a dose of 10 mg/kg with CA521 FALA . Antibody concentrations in serum were determined in Elisa with SARS-CoV-2 (2019-nCoV) spike protein as the capture reagent. b Three healthy rhesus monkeys were administered intravenously at a dose of 50 mg/kg with CA521 FALA . The antibody concentration in serum at different time points were determined in Elisa with SARS-CoV-2 (2019-nCoV) spike protein as the capture reagent. The main PK kinetic parameters were calculated using Phoenix WinNonlin.

    Article Snippet: High binding ELISA plates were coated with 1 μg/mL SARS-CoV-2(2019-nCoV) spike protein (Sino Biological, 40592-V08H) at 4 °C overnight, and then blocked with 1% bovine serum albumin (BSA) in PBS at 37 °C for 1 h, followed by washing five times with PBST.

    Techniques: Concentration Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay

    CA521 FALA inhibited SARS-CoV-2 infection in vitro and in vivo. a CA521 FALA inhibits SARS-CoV-2 pseudovirus infection into Huh7 cells. b CA521 FALA inhibits SARS-CoV-2 pseudovirus infection into hACE2 expressing HEK293T cells. c CA521 FALA inhibits an authentic SARS-CoV-2 strain (BetaCoV/Beijing/IMEBJ01/2020) infection into Vero cells in vitro. Neutralizing activity of mAbs was measured using a standard plaque reduction neutralization with Vero cells. PRNT50 values were determined using non-linear regression analysis. d , e CA521 FALA exited therapeutic efficacy in SARS-CoV-2 susceptible mice. BALB/c mice who received a SARS-CoV-2 mouse-adapted strain (MASCp6) challenge were administered intraperitoneally with a single dose of 20 mg/kg of CA521 FALA ( n = 4) or PBS ( n = 6) in a therapeutic setting. The level of viral RNA was detected in the lung ( d ) and trachea ( e ) at 3 days post infection (3dpi) with a Quantitative PCR assay. f , g Histopathological analysis of lung samples from PBS group or CA521 FALA group at 3 dpi. Scale bar: 100 μm.

    Journal: Communications Biology

    Article Title: Structure and function analysis of a potent human neutralizing antibody CA521FALA against SARS-CoV-2

    doi: 10.1038/s42003-021-02029-w

    Figure Lengend Snippet: CA521 FALA inhibited SARS-CoV-2 infection in vitro and in vivo. a CA521 FALA inhibits SARS-CoV-2 pseudovirus infection into Huh7 cells. b CA521 FALA inhibits SARS-CoV-2 pseudovirus infection into hACE2 expressing HEK293T cells. c CA521 FALA inhibits an authentic SARS-CoV-2 strain (BetaCoV/Beijing/IMEBJ01/2020) infection into Vero cells in vitro. Neutralizing activity of mAbs was measured using a standard plaque reduction neutralization with Vero cells. PRNT50 values were determined using non-linear regression analysis. d , e CA521 FALA exited therapeutic efficacy in SARS-CoV-2 susceptible mice. BALB/c mice who received a SARS-CoV-2 mouse-adapted strain (MASCp6) challenge were administered intraperitoneally with a single dose of 20 mg/kg of CA521 FALA ( n = 4) or PBS ( n = 6) in a therapeutic setting. The level of viral RNA was detected in the lung ( d ) and trachea ( e ) at 3 days post infection (3dpi) with a Quantitative PCR assay. f , g Histopathological analysis of lung samples from PBS group or CA521 FALA group at 3 dpi. Scale bar: 100 μm.

    Article Snippet: High binding ELISA plates were coated with 1 μg/mL SARS-CoV-2(2019-nCoV) spike protein (Sino Biological, 40592-V08H) at 4 °C overnight, and then blocked with 1% bovine serum albumin (BSA) in PBS at 37 °C for 1 h, followed by washing five times with PBST.

    Techniques: Infection, In Vitro, In Vivo, Expressing, Activity Assay, Neutralization, Mouse Assay, Real-time Polymerase Chain Reaction

    CA521 FALA can block the binding of SARS-CoV-2-RBD to hACE2 receptor and specifically bind Spike of SARS-CoV-2. a CA521 FALA can effectively block RBD binding to ACE2 receptor in ELISA. CA521 FALA and hACE2 protein can block the binding of SARS-CoV-2 RBD and hACE2 with IC50 of 0.343 and 8.887 nM, respectively. Experiments were performed in duplicate, value = mean ± SD. b CA521 FALA could specifically bind to CHO-K1 cells expressing SARS-CoV-2 Spike. SARS-CoV-2 Spike protein transfected CHO-K1 cells were stained with isotype control, CA521 FALA at a concentration of 0.74 μg/mL. FITC-anti-HuFc secondary antibody was used for flow cytometry. Irrelevant mAb with the same constant region of CA521 FALA was used as an isotype. Experiments were performed in triplicate and one representative data was displayed. c – e CA521 FALA could specifically bind to SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV Spike or MERS-CoV Spike protein in Elisa. CA521 FALA binds SARS-CoV-2 Spike protein with EC50 of 0.014 nM. CA13, which is an anti- SARS-CoV-2 S2 domain mAb, can bind Spike of SARS-CoV-2 and SARS-CoV with EC50 of 0.015 and 0.019 nM. Experiments were performed in triplicate, value = Mean ± SD. f – h The binding kinetics of CA521 FALA were assessed by biolayer Interferometry (BLI) assay using the Octet RED96 system (FortéBio). Trimer protein is from Shuimu BioSciences. Experiments were performed three times and one representative data was displayed.

    Journal: Communications Biology

    Article Title: Structure and function analysis of a potent human neutralizing antibody CA521FALA against SARS-CoV-2

    doi: 10.1038/s42003-021-02029-w

    Figure Lengend Snippet: CA521 FALA can block the binding of SARS-CoV-2-RBD to hACE2 receptor and specifically bind Spike of SARS-CoV-2. a CA521 FALA can effectively block RBD binding to ACE2 receptor in ELISA. CA521 FALA and hACE2 protein can block the binding of SARS-CoV-2 RBD and hACE2 with IC50 of 0.343 and 8.887 nM, respectively. Experiments were performed in duplicate, value = mean ± SD. b CA521 FALA could specifically bind to CHO-K1 cells expressing SARS-CoV-2 Spike. SARS-CoV-2 Spike protein transfected CHO-K1 cells were stained with isotype control, CA521 FALA at a concentration of 0.74 μg/mL. FITC-anti-HuFc secondary antibody was used for flow cytometry. Irrelevant mAb with the same constant region of CA521 FALA was used as an isotype. Experiments were performed in triplicate and one representative data was displayed. c – e CA521 FALA could specifically bind to SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV Spike or MERS-CoV Spike protein in Elisa. CA521 FALA binds SARS-CoV-2 Spike protein with EC50 of 0.014 nM. CA13, which is an anti- SARS-CoV-2 S2 domain mAb, can bind Spike of SARS-CoV-2 and SARS-CoV with EC50 of 0.015 and 0.019 nM. Experiments were performed in triplicate, value = Mean ± SD. f – h The binding kinetics of CA521 FALA were assessed by biolayer Interferometry (BLI) assay using the Octet RED96 system (FortéBio). Trimer protein is from Shuimu BioSciences. Experiments were performed three times and one representative data was displayed.

    Article Snippet: High binding ELISA plates were coated with 1 μg/mL SARS-CoV-2(2019-nCoV) spike protein (Sino Biological, 40592-V08H) at 4 °C overnight, and then blocked with 1% bovine serum albumin (BSA) in PBS at 37 °C for 1 h, followed by washing five times with PBST.

    Techniques: Blocking Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Expressing, Transfection, Staining, Concentration Assay, Flow Cytometry