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  • 96
    Millipore dimethylglyoxime
    Dimethylglyoxime, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Abcam rabbit anti myelin basic protein
    Rabbit Anti Myelin Basic Protein, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mbp  (Abcam)
    86
    Abcam mbp
    Mbp, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    FUJIFILM rabbit anti iba1
    Astrocytes and microglia retain a normal density despite aberrant axonal and myelin distribution. a-b , Low magnification ( a ) and high magnification ( b ) images of Aldh1l1-positive astrocytes in P30 mouse cortex, showing similar cell densities in layer I heterotopia (right images, arrowheads) compared to corresponding contralateral control regions (left images). c , Quantifications using P30 mouse forebrain tissue showing no significant difference in astrocyte cell density (Wilcoxon non-parametric matched-pairs, signed rank test, n = 6 animals; ns, no significance). d-e Low magnification ( d ) and high magnification ( e ) images of <t>Iba1-immunolabelled</t> P30 mouse forebrain, showing no difference in microglia cell densities between heterotopia (right images, arrowheads) and contralateral control regions (left images). f , Quantifications using P30 mouse forebrain tissue demonstrating no significant difference in microglia cell density between layer I heterotopia and corresponding contralateral control regions (Wilcoxon matched-pairs, signed rank non-parametric test, n = 6 animals; ns, no significance). For graphs in ( c ) and ( f ), each point represents an embryonically-induced layer I heterotopion or control region from one animal, with horizontal lines and accompanying error bars denoting mean and s.e.m., respectively. Descriptive statistics are given in Supplementary File 1. HTP, heterotopia. Scale bars, 100 µm ( a ), 20 µm ( b ), 100 µm ( d ), and 20 µm ( e ).
    Rabbit Anti Iba1, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Astrocytes and microglia retain a normal density despite aberrant axonal and myelin distribution. a-b , Low magnification ( a ) and high magnification ( b ) images of Aldh1l1-positive astrocytes in P30 mouse cortex, showing similar cell densities in layer I heterotopia (right images, arrowheads) compared to corresponding contralateral control regions (left images). c , Quantifications using P30 mouse forebrain tissue showing no significant difference in astrocyte cell density (Wilcoxon non-parametric matched-pairs, signed rank test, n = 6 animals; ns, no significance). d-e Low magnification ( d ) and high magnification ( e ) images of Iba1-immunolabelled P30 mouse forebrain, showing no difference in microglia cell densities between heterotopia (right images, arrowheads) and contralateral control regions (left images). f , Quantifications using P30 mouse forebrain tissue demonstrating no significant difference in microglia cell density between layer I heterotopia and corresponding contralateral control regions (Wilcoxon matched-pairs, signed rank non-parametric test, n = 6 animals; ns, no significance). For graphs in ( c ) and ( f ), each point represents an embryonically-induced layer I heterotopion or control region from one animal, with horizontal lines and accompanying error bars denoting mean and s.e.m., respectively. Descriptive statistics are given in Supplementary File 1. HTP, heterotopia. Scale bars, 100 µm ( a ), 20 µm ( b ), 100 µm ( d ), and 20 µm ( e ).

    Journal: bioRxiv

    Article Title: Aberrant axonal pathfinding and exuberant myelination in an inducible model of neocortical heterotopia

    doi: 10.1101/2020.03.11.987388

    Figure Lengend Snippet: Astrocytes and microglia retain a normal density despite aberrant axonal and myelin distribution. a-b , Low magnification ( a ) and high magnification ( b ) images of Aldh1l1-positive astrocytes in P30 mouse cortex, showing similar cell densities in layer I heterotopia (right images, arrowheads) compared to corresponding contralateral control regions (left images). c , Quantifications using P30 mouse forebrain tissue showing no significant difference in astrocyte cell density (Wilcoxon non-parametric matched-pairs, signed rank test, n = 6 animals; ns, no significance). d-e Low magnification ( d ) and high magnification ( e ) images of Iba1-immunolabelled P30 mouse forebrain, showing no difference in microglia cell densities between heterotopia (right images, arrowheads) and contralateral control regions (left images). f , Quantifications using P30 mouse forebrain tissue demonstrating no significant difference in microglia cell density between layer I heterotopia and corresponding contralateral control regions (Wilcoxon matched-pairs, signed rank non-parametric test, n = 6 animals; ns, no significance). For graphs in ( c ) and ( f ), each point represents an embryonically-induced layer I heterotopion or control region from one animal, with horizontal lines and accompanying error bars denoting mean and s.e.m., respectively. Descriptive statistics are given in Supplementary File 1. HTP, heterotopia. Scale bars, 100 µm ( a ), 20 µm ( b ), 100 µm ( d ), and 20 µm ( e ).

    Article Snippet: The primary antibodies used were: rabbit anti-NeuN (Abcam, Cat# ab177487, 1:3000), mouse anti-NeuN (Abcam, Cat# ab104224, 1:1000), mouse anti-Myelin CNPase (clone SMI 91, Biolegend, Cat# 836404, 1:1000), rabbit anti-Cux1 (Novus Biologicals, Cat# NBP2-13883, 1:100), mouse anti-Tle4 (E-10) Alexa Fluor 647 (Santa Cruz Biotechnology, Cat# sc-365406 AF647, 1:100), mouse anti-GAD-67 (EMD Millipore, Cat# MAB5406, 1:1000), rabbit anti-Iba1 (Wako, Cat# 019-19741, 1:600), mouse anti-Aldh1l1 (clone N103/39, NeuroMab, Cat# 75-140, 1:500), chicken anti-Neurofilament NF-H (EnCor, Cat# CPCA-NF-H, 1:500), chicken anti-GFP (Abcam, Cat# 13970, 1:500), and rabbit anti-Myelin Basic Protein (MBP; Abcam, Cat# 40390, 1:1000).

    Techniques: