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    Sino Biological mers cov spike protein
    ( a ) Blue resonance peak: bare gold surface before the functionalization. Red resonance peak: red shifted resonance due to the MIP layer before template extraction with trypsin enzyme 4.2 × 10 −8 M in buffer phosphate pH 7.4 and sodium dodecyl sulfate (SDS) 5%. Magenta resonance peak: Blue shift due to the freeing of sites from the template protein. ( b ) Response curves of <t>Sars-Cov-2</t> Spike S1 subunit-MIP at different concentrations of protein. ( c ) SARS-CoV-2 Spike protein dose-response curve with the Hill fitting of the data. ( d ) Specificity test: sensor’s responses for <t>MERS-CoV</t> Spike protein and SARS-CoV-2 Spike protein, both with a concentration of 1 µM in UTM buffer.
    Mers Cov Spike Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov spike protein/product/Sino Biological
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov spike protein - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    96
    Sino Biological rabbit monoclonal antibody
    ( a ) Blue resonance peak: bare gold surface before the functionalization. Red resonance peak: red shifted resonance due to the MIP layer before template extraction with trypsin enzyme 4.2 × 10 −8 M in buffer phosphate pH 7.4 and sodium dodecyl sulfate (SDS) 5%. Magenta resonance peak: Blue shift due to the freeing of sites from the template protein. ( b ) Response curves of <t>Sars-Cov-2</t> Spike S1 subunit-MIP at different concentrations of protein. ( c ) SARS-CoV-2 Spike protein dose-response curve with the Hill fitting of the data. ( d ) Specificity test: sensor’s responses for <t>MERS-CoV</t> Spike protein and SARS-CoV-2 Spike protein, both with a concentration of 1 µM in UTM buffer.
    Rabbit Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibody/product/Sino Biological
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibody - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    Image Search Results


    ( a ) Blue resonance peak: bare gold surface before the functionalization. Red resonance peak: red shifted resonance due to the MIP layer before template extraction with trypsin enzyme 4.2 × 10 −8 M in buffer phosphate pH 7.4 and sodium dodecyl sulfate (SDS) 5%. Magenta resonance peak: Blue shift due to the freeing of sites from the template protein. ( b ) Response curves of Sars-Cov-2 Spike S1 subunit-MIP at different concentrations of protein. ( c ) SARS-CoV-2 Spike protein dose-response curve with the Hill fitting of the data. ( d ) Specificity test: sensor’s responses for MERS-CoV Spike protein and SARS-CoV-2 Spike protein, both with a concentration of 1 µM in UTM buffer.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers

    doi: 10.3390/s21051681

    Figure Lengend Snippet: ( a ) Blue resonance peak: bare gold surface before the functionalization. Red resonance peak: red shifted resonance due to the MIP layer before template extraction with trypsin enzyme 4.2 × 10 −8 M in buffer phosphate pH 7.4 and sodium dodecyl sulfate (SDS) 5%. Magenta resonance peak: Blue shift due to the freeing of sites from the template protein. ( b ) Response curves of Sars-Cov-2 Spike S1 subunit-MIP at different concentrations of protein. ( c ) SARS-CoV-2 Spike protein dose-response curve with the Hill fitting of the data. ( d ) Specificity test: sensor’s responses for MERS-CoV Spike protein and SARS-CoV-2 Spike protein, both with a concentration of 1 µM in UTM buffer.

    Article Snippet: The SARS-CoV-2 (2019-nCoV) Spike protein (S1 subunit, His-Tag) and MERS-CoV Spike protein (S1 subunit, His-Tag) were from Sino Biological (Düsseldorfer, Germany).

    Techniques: Concentration Assay

    Incorporation of SARS-CoV-2 S protein into pseudovirions. a Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b – f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g – j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

    doi: 10.1038/s41467-020-15562-9

    Figure Lengend Snippet: Incorporation of SARS-CoV-2 S protein into pseudovirions. a Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b – f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g – j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file.

    Article Snippet: MHV S proteins were detected using polyclonal goat anti-MHV S antibody AO4 (1:2000); SARS-CoV S proteins were blotted with either polyclonal anti-SARS S1 antibodies T62 (1:2000) (Sinobiological Inc, Beijing, China) or mouse monoclonal against SARS S1 antibody MM02 (1:1000) (Sinobiological Inc, Beijing, China), MERS-CoV and SARS-CoV-2 S proteins were detected using mouse monoclonal anti-MERS S (1:1000) (Sinobiological Inc, Beijing, China) and anti-FLAG M2 antibody (1:1000) (Sigma, St. Louis, MO, USA), respectively.

    Techniques: Binding Assay, Western Blot, Transfection, Plasmid Preparation

    Entry and receptor of SARS-CoV-2 S pseudovirons. a , b Entry of SARS-CoV-2 S pseudovirions on indicated cell lines. Cells from human and animal origin were inoculated with SARS-CoV-2 S (red), SARS-CoV S (blue), or VSV-G (gray) pseudovirions. At 48 h post inoculation, transduction efficiency was measured according to luciferase activities. RS, Rhinolophus sinicus bat embryonic fibroblast; BHK/hAPN, BHK cells stably expressing hAPN, the hCoV-229E receptor; 293/hACE2, 293 cells stably expressing hACE2, the SARS-CoV receptor; HeLa/hDPP4, HeLa cells stably expressing hDPP4, the MERS-CoV receptor. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Binding of SARS-CoV S and SARS-CoV-2 S proteins to soluble hACE2. HEK293T cells transiently expressing SARS-CoV and SARS-CoV-2 S proteins were incubated with the soluble hACE2 on ice, followed by polyclonal goat anti-hACE2 antibody. Cells were analyzed by flow cytometry. The experiments were repeated at least three times. d Inhibition of SARS-CoV-2 S pseudovirion entry by soluble hACE2. SARS-CoV S, SARS-CoV-2 S, or VSV-G pseudovirions were pre-incubated with soluble hACE2, then mixture were added to 293/hACE2 cells. Cells were lysed 40 h later and pseudoviral transduction was measured. Experiments were done twice and one representative is shown. Error bars indicate SEM of technical triplicates. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

    doi: 10.1038/s41467-020-15562-9

    Figure Lengend Snippet: Entry and receptor of SARS-CoV-2 S pseudovirons. a , b Entry of SARS-CoV-2 S pseudovirions on indicated cell lines. Cells from human and animal origin were inoculated with SARS-CoV-2 S (red), SARS-CoV S (blue), or VSV-G (gray) pseudovirions. At 48 h post inoculation, transduction efficiency was measured according to luciferase activities. RS, Rhinolophus sinicus bat embryonic fibroblast; BHK/hAPN, BHK cells stably expressing hAPN, the hCoV-229E receptor; 293/hACE2, 293 cells stably expressing hACE2, the SARS-CoV receptor; HeLa/hDPP4, HeLa cells stably expressing hDPP4, the MERS-CoV receptor. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Binding of SARS-CoV S and SARS-CoV-2 S proteins to soluble hACE2. HEK293T cells transiently expressing SARS-CoV and SARS-CoV-2 S proteins were incubated with the soluble hACE2 on ice, followed by polyclonal goat anti-hACE2 antibody. Cells were analyzed by flow cytometry. The experiments were repeated at least three times. d Inhibition of SARS-CoV-2 S pseudovirion entry by soluble hACE2. SARS-CoV S, SARS-CoV-2 S, or VSV-G pseudovirions were pre-incubated with soluble hACE2, then mixture were added to 293/hACE2 cells. Cells were lysed 40 h later and pseudoviral transduction was measured. Experiments were done twice and one representative is shown. Error bars indicate SEM of technical triplicates. Source data are provided as a Source Data file.

    Article Snippet: MHV S proteins were detected using polyclonal goat anti-MHV S antibody AO4 (1:2000); SARS-CoV S proteins were blotted with either polyclonal anti-SARS S1 antibodies T62 (1:2000) (Sinobiological Inc, Beijing, China) or mouse monoclonal against SARS S1 antibody MM02 (1:1000) (Sinobiological Inc, Beijing, China), MERS-CoV and SARS-CoV-2 S proteins were detected using mouse monoclonal anti-MERS S (1:1000) (Sinobiological Inc, Beijing, China) and anti-FLAG M2 antibody (1:1000) (Sigma, St. Louis, MO, USA), respectively.

    Techniques: Transduction, Luciferase, Stable Transfection, Expressing, Binding Assay, Incubation, Flow Cytometry, Inhibition

    Endocytosis of SARS-CoV-2 S pseudovirions on 293/hACE2 cells. a Inhibition of entry of SARS-CoV-2 S pseudovirion on 293/hACE2 by lysosomotropic agents (20 mM NH 4 Cl and 100 nM bafilomycin A). b Inhibition of entry of SARS-CoV, MERS-CoV, and MHV S pseudovirions by a PIKfyve inhibitor apilimod. HeLa/mCEACAM, 293/hACE2, HeLa/hDPP4 cells were pretreated with different concentrations of apilimod and transduced with MHV S, SARS-CoV S, MERS-CoV S pseudovirions, respectively. The luciferase activity was measured 40 h post transduction. VSV-G pseudovirions were used as a control. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Inhibition of MHV A59 infection by apilimod. The 17Cl.1 cells were pretreated with 3, 10, 30, 100, 300 nM apilimod for 30 min and infected by MHV A59 at MOI = 0.01. Viral infection and cell viability were determined by using qPCR and MTT assay, respectively. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. d , e Inhibition of entry of SARS-CoV-2 S protein pseudovirions by apilimod, YM201636, and tetrandrine. HEK 293/hACE2 cells were pretreated with either apilimod ( d ), YM201636 ( e ), or tetrandrine ( f ), then inoculated with SARS-CoV-2 S pseudovirons in the presence of drug. The luciferase activity were measured 40 h post transduction. YM201636, PIKfyve inhibitor; tetrandrine, TPC2 inhibitor. The experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM of technical triplicates. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

    doi: 10.1038/s41467-020-15562-9

    Figure Lengend Snippet: Endocytosis of SARS-CoV-2 S pseudovirions on 293/hACE2 cells. a Inhibition of entry of SARS-CoV-2 S pseudovirion on 293/hACE2 by lysosomotropic agents (20 mM NH 4 Cl and 100 nM bafilomycin A). b Inhibition of entry of SARS-CoV, MERS-CoV, and MHV S pseudovirions by a PIKfyve inhibitor apilimod. HeLa/mCEACAM, 293/hACE2, HeLa/hDPP4 cells were pretreated with different concentrations of apilimod and transduced with MHV S, SARS-CoV S, MERS-CoV S pseudovirions, respectively. The luciferase activity was measured 40 h post transduction. VSV-G pseudovirions were used as a control. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Inhibition of MHV A59 infection by apilimod. The 17Cl.1 cells were pretreated with 3, 10, 30, 100, 300 nM apilimod for 30 min and infected by MHV A59 at MOI = 0.01. Viral infection and cell viability were determined by using qPCR and MTT assay, respectively. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. d , e Inhibition of entry of SARS-CoV-2 S protein pseudovirions by apilimod, YM201636, and tetrandrine. HEK 293/hACE2 cells were pretreated with either apilimod ( d ), YM201636 ( e ), or tetrandrine ( f ), then inoculated with SARS-CoV-2 S pseudovirons in the presence of drug. The luciferase activity were measured 40 h post transduction. YM201636, PIKfyve inhibitor; tetrandrine, TPC2 inhibitor. The experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM of technical triplicates. Source data are provided as a Source Data file.

    Article Snippet: MHV S proteins were detected using polyclonal goat anti-MHV S antibody AO4 (1:2000); SARS-CoV S proteins were blotted with either polyclonal anti-SARS S1 antibodies T62 (1:2000) (Sinobiological Inc, Beijing, China) or mouse monoclonal against SARS S1 antibody MM02 (1:1000) (Sinobiological Inc, Beijing, China), MERS-CoV and SARS-CoV-2 S proteins were detected using mouse monoclonal anti-MERS S (1:1000) (Sinobiological Inc, Beijing, China) and anti-FLAG M2 antibody (1:1000) (Sigma, St. Louis, MO, USA), respectively.

    Techniques: Inhibition, Transduction, Luciferase, Activity Assay, Infection, Real-time Polymerase Chain Reaction, MTT Assay

    Workflow for generation of human monoclonal antibodies (mAbs) by cloning antibody genes from primary human B cells. Step 1. B cells were isolated from peripheral blood mononuclear cells of a recovered Middle East respiratory syndrome coronavirus (MERS-CoV) patient and then cultured in the 96-well plates in the presence of 3T3 cells, human interleukin (hIL)-2, CpG2006, and hIL-21 for 10 days. Step 2. Culture supernatants were used to detect MERS-CoV spike (MERS-CoV S) for binding activities using enzyme-linked immunosorbent assay. Step 3. Positive wells were used to detect neutralization activities against MERS-CoV S pseudoviruses. Step 4. The variable regions were cloned into expression vectors and analyzed by sequencing technology. Step 5. The selected VH and VL/VK clonal genes were transiently cotransfected into HEK-293T cells from the same well. Step 6. The culture supernatants were detected against MERS-CoV S pseudoviruses for neutralization activities. Step 7. The neutralizing mAbs were purified, and the immunological function was validated. Abbreviations: IGH, immunoglobulin heavy-chain; IGL, immunoglobulin light chain; IGK, immunoglobulin ; IR, inhibition rate.

    Journal: The Journal of Infectious Diseases

    Article Title: Ultrapotent Human Neutralizing Antibody Repertoires Against Middle East Respiratory Syndrome Coronavirus From a Recovered Patient

    doi: 10.1093/infdis/jiy311

    Figure Lengend Snippet: Workflow for generation of human monoclonal antibodies (mAbs) by cloning antibody genes from primary human B cells. Step 1. B cells were isolated from peripheral blood mononuclear cells of a recovered Middle East respiratory syndrome coronavirus (MERS-CoV) patient and then cultured in the 96-well plates in the presence of 3T3 cells, human interleukin (hIL)-2, CpG2006, and hIL-21 for 10 days. Step 2. Culture supernatants were used to detect MERS-CoV spike (MERS-CoV S) for binding activities using enzyme-linked immunosorbent assay. Step 3. Positive wells were used to detect neutralization activities against MERS-CoV S pseudoviruses. Step 4. The variable regions were cloned into expression vectors and analyzed by sequencing technology. Step 5. The selected VH and VL/VK clonal genes were transiently cotransfected into HEK-293T cells from the same well. Step 6. The culture supernatants were detected against MERS-CoV S pseudoviruses for neutralization activities. Step 7. The neutralizing mAbs were purified, and the immunological function was validated. Abbreviations: IGH, immunoglobulin heavy-chain; IGL, immunoglobulin light chain; IGK, immunoglobulin ; IR, inhibition rate.

    Article Snippet: The serum Abs displayed high reactivity to MERS-CoV S protein (Sino Biological Inc., Beijing, China) and neutralizing activity as determined by an enzyme-linked immunosorbent assay (ELISA) and MERS-CoV pseudovirus production, neutralization assay [ ].

    Techniques: Clone Assay, Isolation, Cell Culture, Binding Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Expressing, Sequencing, Purification, Inhibition

    The functional verification of Middle East respiratory syndrome (MERS)-GD27 and MERS-GD33 in vitro. (A) Specific interaction between MERS-CoV S protein and MERS-GD27 and MERS-GD33 characterized by biolayer interferometry. The monoclonal antibodies (mAbs) were captured on the 96-well microplate immobilized with anti-hIgG-Fc and tested for binding with gradient concentrations of MERS-coronavirus spike (CoV S) protein. (B) The apparent dissociation constants were calculated and summarized. (C) Neutralizing activities of MERS-GD27 and MERS-GD33 against live MERS-CoV and against plaque formation of Vero-E6 cells by plaque reduction neutralization test. Three different concentrations of Abs (0.001, 0.01, and 0.1 μg/well) were incubated with 30 plaque-forming units/well live MERS-CoV. Cells were stained with crystal violet at the end of treatment, and the plaques were determined. The inhibitory activity is over 50% in the box. (D) Competition studies among MERS-GD27 and MERS-GD33 with other mAbs. The 96-well plate was first coated with MERS-CoV S 24 hours before the competitive binding test. The mixture of Ab-biotins and other antibodies was incubated in the concentration for 50% of maximal effect. Phosphate-buffered saline was used as a blank control. According to the different competitive binding, 3 groups were created (see Supplementary Table S3 ).

    Journal: The Journal of Infectious Diseases

    Article Title: Ultrapotent Human Neutralizing Antibody Repertoires Against Middle East Respiratory Syndrome Coronavirus From a Recovered Patient

    doi: 10.1093/infdis/jiy311

    Figure Lengend Snippet: The functional verification of Middle East respiratory syndrome (MERS)-GD27 and MERS-GD33 in vitro. (A) Specific interaction between MERS-CoV S protein and MERS-GD27 and MERS-GD33 characterized by biolayer interferometry. The monoclonal antibodies (mAbs) were captured on the 96-well microplate immobilized with anti-hIgG-Fc and tested for binding with gradient concentrations of MERS-coronavirus spike (CoV S) protein. (B) The apparent dissociation constants were calculated and summarized. (C) Neutralizing activities of MERS-GD27 and MERS-GD33 against live MERS-CoV and against plaque formation of Vero-E6 cells by plaque reduction neutralization test. Three different concentrations of Abs (0.001, 0.01, and 0.1 μg/well) were incubated with 30 plaque-forming units/well live MERS-CoV. Cells were stained with crystal violet at the end of treatment, and the plaques were determined. The inhibitory activity is over 50% in the box. (D) Competition studies among MERS-GD27 and MERS-GD33 with other mAbs. The 96-well plate was first coated with MERS-CoV S 24 hours before the competitive binding test. The mixture of Ab-biotins and other antibodies was incubated in the concentration for 50% of maximal effect. Phosphate-buffered saline was used as a blank control. According to the different competitive binding, 3 groups were created (see Supplementary Table S3 ).

    Article Snippet: The serum Abs displayed high reactivity to MERS-CoV S protein (Sino Biological Inc., Beijing, China) and neutralizing activity as determined by an enzyme-linked immunosorbent assay (ELISA) and MERS-CoV pseudovirus production, neutralization assay [ ].

    Techniques: Functional Assay, In Vitro, Binding Assay, Plaque Reduction Neutralization Test, Incubation, Staining, Activity Assay, Concentration Assay