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86
Becton Dickinson hla dr buv 395
Hla Dr Buv 395, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Yeasen Biotechnology annexin v fitc pi
BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin <t>V-FITC/PI</t> apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated
Annexin V Fitc Pi, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Yeasen Biotechnology pe annexin v
BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin <t>V-FITC/PI</t> apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated
Pe Annexin V, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Yeasen Biotechnology binding buffer
BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin <t>V-FITC/PI</t> apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated
Binding Buffer, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
NR2F1-AS1 enhanced ECM degeneration and human NP cell <t>apoptosis.</t>
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated

Journal: Journal of Nanobiotechnology

Article Title: In vivo therapy of osteosarcoma using anion transporters-based supramolecular drugs

doi: 10.1186/s12951-023-02270-x

Figure Lengend Snippet: BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated

Article Snippet: For cell apoptosis assay or cell cycle analysis, cells were trypsinized, centrifuged, washed with ice-cold PBS and stained with Annexin V-FITC/PI (40,302, YEASEN, China) or PI (40,301, YEASEN, China) for 15–30 min according to the instructions.

Techniques: Migration, In Vitro, Cell Counting, EdU Assay, Flow Cytometry, Wound Healing Assay, Expressing, Western Blot, Comparison

The self-assembled OTP-BTFPTU supramolecular liposomes inhibits the proliferation and migration of HOS cells in vitro. ( A ) Schematic illustration of osteosarcoma targeting peptide (OTP)-BTFPTU supramolecular liposomes prepared through self-assembly. ( B ) The TEM images of OTP-BTFPTU liposomes. Scale bar, 100 nm. ( C ) FITC (green), DID (red) and DAPI (blue) immunofluorescence of HOS cells with or without OTP-BTFPTU (2 µM) liposomes treatment for 3 h. Scale bar, 20 μm. ( D ) Cell viability of HOS cells with OTP-PTU, OTP-TFPTU or OTP-BTFPTU liposomes treatment for 24 h using Cell Counting Kit-8 assay. ( E ) HOS cells were treated with different concentrations of OTP-BTFPTU liposomes for 24 h and the cell proliferation was characterized by EdU assay. Scale bar, 200 μm. ( F ) Flow cytometry to evaluate apoptosis of HOS cells with or without OTP-BTFPTU liposomes (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. ( G ) Quantification of apoptosis rate of HOS cells. ( H ) Cell migration abilities of HOS cells treated with different concentrations of OTP-BTFPTU liposomes for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( I ) Quantification of the migration abilities of HOS cells. ( J ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of OTP-BTFPTU liposomes for 24 h. Scale bar, 50 μm. ( K ) Quantification of the wound closure rate. ( L ) The protein expression of N-Cadherin, Vimentin and c-Myc was measured by Western blot analysis in HOS cells treated with varying concentrations of OTP-BTFPTU liposomes for 24 h. ( M ) Quantification and normalization of the gray levels of N-Cadherin, Vimentin and c-Myc proteins to that of GAPDH in HOS cells using Image J. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated

Journal: Journal of Nanobiotechnology

Article Title: In vivo therapy of osteosarcoma using anion transporters-based supramolecular drugs

doi: 10.1186/s12951-023-02270-x

Figure Lengend Snippet: The self-assembled OTP-BTFPTU supramolecular liposomes inhibits the proliferation and migration of HOS cells in vitro. ( A ) Schematic illustration of osteosarcoma targeting peptide (OTP)-BTFPTU supramolecular liposomes prepared through self-assembly. ( B ) The TEM images of OTP-BTFPTU liposomes. Scale bar, 100 nm. ( C ) FITC (green), DID (red) and DAPI (blue) immunofluorescence of HOS cells with or without OTP-BTFPTU (2 µM) liposomes treatment for 3 h. Scale bar, 20 μm. ( D ) Cell viability of HOS cells with OTP-PTU, OTP-TFPTU or OTP-BTFPTU liposomes treatment for 24 h using Cell Counting Kit-8 assay. ( E ) HOS cells were treated with different concentrations of OTP-BTFPTU liposomes for 24 h and the cell proliferation was characterized by EdU assay. Scale bar, 200 μm. ( F ) Flow cytometry to evaluate apoptosis of HOS cells with or without OTP-BTFPTU liposomes (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. ( G ) Quantification of apoptosis rate of HOS cells. ( H ) Cell migration abilities of HOS cells treated with different concentrations of OTP-BTFPTU liposomes for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( I ) Quantification of the migration abilities of HOS cells. ( J ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of OTP-BTFPTU liposomes for 24 h. Scale bar, 50 μm. ( K ) Quantification of the wound closure rate. ( L ) The protein expression of N-Cadherin, Vimentin and c-Myc was measured by Western blot analysis in HOS cells treated with varying concentrations of OTP-BTFPTU liposomes for 24 h. ( M ) Quantification and normalization of the gray levels of N-Cadherin, Vimentin and c-Myc proteins to that of GAPDH in HOS cells using Image J. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated

Article Snippet: For cell apoptosis assay or cell cycle analysis, cells were trypsinized, centrifuged, washed with ice-cold PBS and stained with Annexin V-FITC/PI (40,302, YEASEN, China) or PI (40,301, YEASEN, China) for 15–30 min according to the instructions.

Techniques: Liposomes, Migration, In Vitro, Immunofluorescence, Cell Counting, EdU Assay, Flow Cytometry, Wound Healing Assay, Expressing, Western Blot, Comparison

NR2F1-AS1 enhanced ECM degeneration and human NP cell apoptosis.

Journal: Bioengineered

Article Title: LncRNA nuclear receptor subfamily 2 group F member 1 antisense RNA 1 (NR2F1-AS1) aggravates nucleus pulposus cell apoptosis and extracellular matrix degradation

doi: 10.1080/21655979.2021.2016087

Figure Lengend Snippet: NR2F1-AS1 enhanced ECM degeneration and human NP cell apoptosis.

Article Snippet: Annexin V-FITC-PI apoptosis detection kit (Cat:40,302, Yeasen, Shanghai, China) was used for evaluating apoptosis.

Techniques:

miR-145-5p dampened ECM degeneration and NP cell apoptosis.

Journal: Bioengineered

Article Title: LncRNA nuclear receptor subfamily 2 group F member 1 antisense RNA 1 (NR2F1-AS1) aggravates nucleus pulposus cell apoptosis and extracellular matrix degradation

doi: 10.1080/21655979.2021.2016087

Figure Lengend Snippet: miR-145-5p dampened ECM degeneration and NP cell apoptosis.

Article Snippet: Annexin V-FITC-PI apoptosis detection kit (Cat:40,302, Yeasen, Shanghai, China) was used for evaluating apoptosis.

Techniques:

NR2F1-AS1 strengthened the ECM denaturation and apoptosis in human NP cells via miR-145-5p/FOXO1.

Journal: Bioengineered

Article Title: LncRNA nuclear receptor subfamily 2 group F member 1 antisense RNA 1 (NR2F1-AS1) aggravates nucleus pulposus cell apoptosis and extracellular matrix degradation

doi: 10.1080/21655979.2021.2016087

Figure Lengend Snippet: NR2F1-AS1 strengthened the ECM denaturation and apoptosis in human NP cells via miR-145-5p/FOXO1.

Article Snippet: Annexin V-FITC-PI apoptosis detection kit (Cat:40,302, Yeasen, Shanghai, China) was used for evaluating apoptosis.

Techniques: