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    Becton Dickinson hla dr buv 395
    Hla Dr Buv 395, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hla dr buv 395/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hla dr buv 395 - by Bioz Stars, 2024-02
    86/100 stars
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    86
    Yeasen Biotechnology annexin v fitc pi
    BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin <t>V-FITC/PI</t> apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated
    Annexin V Fitc Pi, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi/product/Yeasen Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc pi - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

    86
    Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
    BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin <t>V-FITC/PI</t> apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Yeasen Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-02
    86/100 stars
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    86
    Beta Pharma polymyxin b sulphate
    Heatmaps showing protein expression changes of AB5075 in A ) stress response; B ) metal acquisition; C ) multidrug efflux; D ) outer membrane; E ) carbon and energy metabolism; F ) amino acid metabolism; and G ) protein biosynthesis in respective comparison groups. The colour code shows log 2 FC values of differentially expressed proteins with FDR <0.05 compared to that of untreated controls. Grey indicates protein expression exceeded an FDR of 0.05. Data are presented as mean of three biological replicates per experimental group. AB, wild-type A. baumannii AB5075; PMB, polymyxin B; THP-1-dMs, macrophage-like THP-1 cells.
    Polymyxin B Sulphate, supplied by Beta Pharma, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymyxin b sulphate/product/Beta Pharma
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polymyxin b sulphate - by Bioz Stars, 2024-02
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    Image Search Results


    BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated

    Journal: Journal of Nanobiotechnology

    Article Title: In vivo therapy of osteosarcoma using anion transporters-based supramolecular drugs

    doi: 10.1186/s12951-023-02270-x

    Figure Lengend Snippet: BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. ( A ) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. ( B ) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. ( C ) Quantification of the colony number. ( D ) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. ( E ) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. ( F ) Quantification of apoptosis rate of HOS and 143B cells. ( G ) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( H ) Quantification of the migration abilities of HOS and 143B cells. ( I ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. ( J ) Quantification of the wound closure rate. ( K ) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated

    Article Snippet: For cell apoptosis assay or cell cycle analysis, cells were trypsinized, centrifuged, washed with ice-cold PBS and stained with Annexin V-FITC/PI (40,302, YEASEN, China) or PI (40,301, YEASEN, China) for 15–30 min according to the instructions.

    Techniques: Migration, In Vitro, Cell Counting, EdU Assay, Flow Cytometry, Wound Healing Assay, Expressing, Western Blot, Comparison

    The self-assembled OTP-BTFPTU supramolecular liposomes inhibits the proliferation and migration of HOS cells in vitro. ( A ) Schematic illustration of osteosarcoma targeting peptide (OTP)-BTFPTU supramolecular liposomes prepared through self-assembly. ( B ) The TEM images of OTP-BTFPTU liposomes. Scale bar, 100 nm. ( C ) FITC (green), DID (red) and DAPI (blue) immunofluorescence of HOS cells with or without OTP-BTFPTU (2 µM) liposomes treatment for 3 h. Scale bar, 20 μm. ( D ) Cell viability of HOS cells with OTP-PTU, OTP-TFPTU or OTP-BTFPTU liposomes treatment for 24 h using Cell Counting Kit-8 assay. ( E ) HOS cells were treated with different concentrations of OTP-BTFPTU liposomes for 24 h and the cell proliferation was characterized by EdU assay. Scale bar, 200 μm. ( F ) Flow cytometry to evaluate apoptosis of HOS cells with or without OTP-BTFPTU liposomes (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. ( G ) Quantification of apoptosis rate of HOS cells. ( H ) Cell migration abilities of HOS cells treated with different concentrations of OTP-BTFPTU liposomes for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( I ) Quantification of the migration abilities of HOS cells. ( J ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of OTP-BTFPTU liposomes for 24 h. Scale bar, 50 μm. ( K ) Quantification of the wound closure rate. ( L ) The protein expression of N-Cadherin, Vimentin and c-Myc was measured by Western blot analysis in HOS cells treated with varying concentrations of OTP-BTFPTU liposomes for 24 h. ( M ) Quantification and normalization of the gray levels of N-Cadherin, Vimentin and c-Myc proteins to that of GAPDH in HOS cells using Image J. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated

    Journal: Journal of Nanobiotechnology

    Article Title: In vivo therapy of osteosarcoma using anion transporters-based supramolecular drugs

    doi: 10.1186/s12951-023-02270-x

    Figure Lengend Snippet: The self-assembled OTP-BTFPTU supramolecular liposomes inhibits the proliferation and migration of HOS cells in vitro. ( A ) Schematic illustration of osteosarcoma targeting peptide (OTP)-BTFPTU supramolecular liposomes prepared through self-assembly. ( B ) The TEM images of OTP-BTFPTU liposomes. Scale bar, 100 nm. ( C ) FITC (green), DID (red) and DAPI (blue) immunofluorescence of HOS cells with or without OTP-BTFPTU (2 µM) liposomes treatment for 3 h. Scale bar, 20 μm. ( D ) Cell viability of HOS cells with OTP-PTU, OTP-TFPTU or OTP-BTFPTU liposomes treatment for 24 h using Cell Counting Kit-8 assay. ( E ) HOS cells were treated with different concentrations of OTP-BTFPTU liposomes for 24 h and the cell proliferation was characterized by EdU assay. Scale bar, 200 μm. ( F ) Flow cytometry to evaluate apoptosis of HOS cells with or without OTP-BTFPTU liposomes (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. ( G ) Quantification of apoptosis rate of HOS cells. ( H ) Cell migration abilities of HOS cells treated with different concentrations of OTP-BTFPTU liposomes for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. ( I ) Quantification of the migration abilities of HOS cells. ( J ) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of OTP-BTFPTU liposomes for 24 h. Scale bar, 50 μm. ( K ) Quantification of the wound closure rate. ( L ) The protein expression of N-Cadherin, Vimentin and c-Myc was measured by Western blot analysis in HOS cells treated with varying concentrations of OTP-BTFPTU liposomes for 24 h. ( M ) Quantification and normalization of the gray levels of N-Cadherin, Vimentin and c-Myc proteins to that of GAPDH in HOS cells using Image J. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated

    Article Snippet: For cell apoptosis assay or cell cycle analysis, cells were trypsinized, centrifuged, washed with ice-cold PBS and stained with Annexin V-FITC/PI (40,302, YEASEN, China) or PI (40,301, YEASEN, China) for 15–30 min according to the instructions.

    Techniques: Liposomes, Migration, In Vitro, Immunofluorescence, Cell Counting, EdU Assay, Flow Cytometry, Wound Healing Assay, Expressing, Western Blot, Comparison

    Heatmaps showing protein expression changes of AB5075 in A ) stress response; B ) metal acquisition; C ) multidrug efflux; D ) outer membrane; E ) carbon and energy metabolism; F ) amino acid metabolism; and G ) protein biosynthesis in respective comparison groups. The colour code shows log 2 FC values of differentially expressed proteins with FDR <0.05 compared to that of untreated controls. Grey indicates protein expression exceeded an FDR of 0.05. Data are presented as mean of three biological replicates per experimental group. AB, wild-type A. baumannii AB5075; PMB, polymyxin B; THP-1-dMs, macrophage-like THP-1 cells.

    Journal: PLoS Pathogens

    Article Title: Correlative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and human macrophages

    doi: 10.1371/journal.ppat.1010308

    Figure Lengend Snippet: Heatmaps showing protein expression changes of AB5075 in A ) stress response; B ) metal acquisition; C ) multidrug efflux; D ) outer membrane; E ) carbon and energy metabolism; F ) amino acid metabolism; and G ) protein biosynthesis in respective comparison groups. The colour code shows log 2 FC values of differentially expressed proteins with FDR <0.05 compared to that of untreated controls. Grey indicates protein expression exceeded an FDR of 0.05. Data are presented as mean of three biological replicates per experimental group. AB, wild-type A. baumannii AB5075; PMB, polymyxin B; THP-1-dMs, macrophage-like THP-1 cells.

    Article Snippet: Polymyxin B sulphate (PMB; Catalog: 86–40302) was obtained from Betapharma (Shanghai, China).

    Techniques: Expressing

    Time-kill profiles of AB5075 transposon mutants with disrupted A ) redox stress resistance genes; B ) iron acquisition genes; C ) stringent response regulatory genes following 4 mg/L polymyxin B (PMB) treatment. Data shown as mean ± SD of three biological replicates. Two-way ANOVA with Tukey’s HSD post-hoc test, * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. Dashed line indicates the limit of detection.

    Journal: PLoS Pathogens

    Article Title: Correlative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and human macrophages

    doi: 10.1371/journal.ppat.1010308

    Figure Lengend Snippet: Time-kill profiles of AB5075 transposon mutants with disrupted A ) redox stress resistance genes; B ) iron acquisition genes; C ) stringent response regulatory genes following 4 mg/L polymyxin B (PMB) treatment. Data shown as mean ± SD of three biological replicates. Two-way ANOVA with Tukey’s HSD post-hoc test, * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. Dashed line indicates the limit of detection.

    Article Snippet: Polymyxin B sulphate (PMB; Catalog: 86–40302) was obtained from Betapharma (Shanghai, China).

    Techniques:

    ImageJ was employed for imaging analysis and cell count of AB5075 wild-type (WT) and spoT mutant following polymyxin B (PMB) treatment at 0.25 or 0.5 mg/L, and no treatment (i.e., controls).

    Journal: PLoS Pathogens

    Article Title: Correlative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and human macrophages

    doi: 10.1371/journal.ppat.1010308

    Figure Lengend Snippet: ImageJ was employed for imaging analysis and cell count of AB5075 wild-type (WT) and spoT mutant following polymyxin B (PMB) treatment at 0.25 or 0.5 mg/L, and no treatment (i.e., controls).

    Article Snippet: Polymyxin B sulphate (PMB; Catalog: 86–40302) was obtained from Betapharma (Shanghai, China).

    Techniques: Imaging, Cell Counting, Mutagenesis

    Interacting bacterial growth of AB5075 wild-type (WT) and transposon mutants with disrupted stringent response regulatory genes following MOI 1,000 infection of THP-1-dMs in the A ) absence and B ) presence of 0.25 mg/L polymyxin B (PMB) treatment. Data shown as mean ± SD of three biological replicates compared to that of the wild-type. Two-way ANOVA with Tukey’s HSD post-hoc test, * p <0.05, ** p <0.01.

    Journal: PLoS Pathogens

    Article Title: Correlative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and human macrophages

    doi: 10.1371/journal.ppat.1010308

    Figure Lengend Snippet: Interacting bacterial growth of AB5075 wild-type (WT) and transposon mutants with disrupted stringent response regulatory genes following MOI 1,000 infection of THP-1-dMs in the A ) absence and B ) presence of 0.25 mg/L polymyxin B (PMB) treatment. Data shown as mean ± SD of three biological replicates compared to that of the wild-type. Two-way ANOVA with Tukey’s HSD post-hoc test, * p <0.05, ** p <0.01.

    Article Snippet: Polymyxin B sulphate (PMB; Catalog: 86–40302) was obtained from Betapharma (Shanghai, China).

    Techniques: Infection

    Bacterial load of AB5075 wild-type (WT) and spoT mutant in blood in a mouse bacteremia model with and without polymyxin B (PMB) treatment (4 mg/kg, intravenous). At least three biological replicates per experimental group were examined. Multiple unpaired t -tests, ** p <0.01, *** p <0.001. Dashed line indicates the limit of detection.

    Journal: PLoS Pathogens

    Article Title: Correlative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and human macrophages

    doi: 10.1371/journal.ppat.1010308

    Figure Lengend Snippet: Bacterial load of AB5075 wild-type (WT) and spoT mutant in blood in a mouse bacteremia model with and without polymyxin B (PMB) treatment (4 mg/kg, intravenous). At least three biological replicates per experimental group were examined. Multiple unpaired t -tests, ** p <0.01, *** p <0.001. Dashed line indicates the limit of detection.

    Article Snippet: Polymyxin B sulphate (PMB; Catalog: 86–40302) was obtained from Betapharma (Shanghai, China).

    Techniques: Mutagenesis