39-867 Search Results


teredinibacter turnerae atcc 39867t  (ATCC)
93
ATCC teredinibacter turnerae atcc 39867t
Teredinibacter Turnerae Atcc 39867t, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β2 ar shrna  (Santa Cruz Biotechnology)
85
Santa Cruz Biotechnology β2 ar shrna
β2 Ar Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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β 2 ar sirna  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology β 2 ar sirna
<t>β</t> <t>2</t> -AR is required for CLB-induced p27 accumulation and cell cycle arrest. (A) CLB treatment relocalizes β 2 -AR. Synchronized cells were treated with 100 µM CLB for 12 h, and incubated with antibodies against β 2 -AR, and then with a secondary antibody conjugated to Alexa 488 (green). DAPI was used to label the nucleus (blue). Scale bar, 50 µm. (B) <t>siRNA</t> knockdown of β 2 -AR reverses p27 accumulation. Synchronized cells were transiently transfected with β 2 -AR and control siRNA, and then exposed to 100 µM CLB for 60 h. Expression of p27 was measured, using GAPDH as loading control. Fold expression change is indicated below blots. (C) β 2 -AR knockdown relieves cell cycle arrest. Synchronized cells were transiently transfected with control and β 2 -AR siRNA, treated with 100 µM CLB for 60 h, and analyzed by flow cytometry. Data are mean ± SD (n = 3). *, p < 0.05; ***, p < 0.001.
β 2 Ar Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β 2 ar sirna/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
β 2 ar sirna - by Bioz Stars, 2024-12
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anti mouse mrtfa primary antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti mouse mrtfa primary antibody
<t>β</t> <t>2</t> -AR is required for CLB-induced p27 accumulation and cell cycle arrest. (A) CLB treatment relocalizes β 2 -AR. Synchronized cells were treated with 100 µM CLB for 12 h, and incubated with antibodies against β 2 -AR, and then with a secondary antibody conjugated to Alexa 488 (green). DAPI was used to label the nucleus (blue). Scale bar, 50 µm. (B) <t>siRNA</t> knockdown of β 2 -AR reverses p27 accumulation. Synchronized cells were transiently transfected with β 2 -AR and control siRNA, and then exposed to 100 µM CLB for 60 h. Expression of p27 was measured, using GAPDH as loading control. Fold expression change is indicated below blots. (C) β 2 -AR knockdown relieves cell cycle arrest. Synchronized cells were transiently transfected with control and β 2 -AR siRNA, treated with 100 µM CLB for 60 h, and analyzed by flow cytometry. Data are mean ± SD (n = 3). *, p < 0.05; ***, p < 0.001.
Anti Mouse Mrtfa Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mouse mrtfa primary antibody/product/Santa Cruz Biotechnology
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kat6a active motif 39867  (Active Motif)
86
Active Motif kat6a active motif 39867
<t>β</t> <t>2</t> -AR is required for CLB-induced p27 accumulation and cell cycle arrest. (A) CLB treatment relocalizes β 2 -AR. Synchronized cells were treated with 100 µM CLB for 12 h, and incubated with antibodies against β 2 -AR, and then with a secondary antibody conjugated to Alexa 488 (green). DAPI was used to label the nucleus (blue). Scale bar, 50 µm. (B) <t>siRNA</t> knockdown of β 2 -AR reverses p27 accumulation. Synchronized cells were transiently transfected with β 2 -AR and control siRNA, and then exposed to 100 µM CLB for 60 h. Expression of p27 was measured, using GAPDH as loading control. Fold expression change is indicated below blots. (C) β 2 -AR knockdown relieves cell cycle arrest. Synchronized cells were transiently transfected with control and β 2 -AR siRNA, treated with 100 µM CLB for 60 h, and analyzed by flow cytometry. Data are mean ± SD (n = 3). *, p < 0.05; ***, p < 0.001.
Kat6a Active Motif 39867, supplied by Active Motif, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


β 2 -AR is required for CLB-induced p27 accumulation and cell cycle arrest. (A) CLB treatment relocalizes β 2 -AR. Synchronized cells were treated with 100 µM CLB for 12 h, and incubated with antibodies against β 2 -AR, and then with a secondary antibody conjugated to Alexa 488 (green). DAPI was used to label the nucleus (blue). Scale bar, 50 µm. (B) siRNA knockdown of β 2 -AR reverses p27 accumulation. Synchronized cells were transiently transfected with β 2 -AR and control siRNA, and then exposed to 100 µM CLB for 60 h. Expression of p27 was measured, using GAPDH as loading control. Fold expression change is indicated below blots. (C) β 2 -AR knockdown relieves cell cycle arrest. Synchronized cells were transiently transfected with control and β 2 -AR siRNA, treated with 100 µM CLB for 60 h, and analyzed by flow cytometry. Data are mean ± SD (n = 3). *, p < 0.05; ***, p < 0.001.

Journal: International Journal of Biological Sciences

Article Title: Clenbuterol Induces Cell Cycle Arrest in C2C12 Myoblasts by Delaying p27 Degradation through β-arrestin 2 Signaling

doi: 10.7150/ijbs.17948

Figure Lengend Snippet: β 2 -AR is required for CLB-induced p27 accumulation and cell cycle arrest. (A) CLB treatment relocalizes β 2 -AR. Synchronized cells were treated with 100 µM CLB for 12 h, and incubated with antibodies against β 2 -AR, and then with a secondary antibody conjugated to Alexa 488 (green). DAPI was used to label the nucleus (blue). Scale bar, 50 µm. (B) siRNA knockdown of β 2 -AR reverses p27 accumulation. Synchronized cells were transiently transfected with β 2 -AR and control siRNA, and then exposed to 100 µM CLB for 60 h. Expression of p27 was measured, using GAPDH as loading control. Fold expression change is indicated below blots. (C) β 2 -AR knockdown relieves cell cycle arrest. Synchronized cells were transiently transfected with control and β 2 -AR siRNA, treated with 100 µM CLB for 60 h, and analyzed by flow cytometry. Data are mean ± SD (n = 3). *, p < 0.05; ***, p < 0.001.

Article Snippet: CLB (Sigma-Aldrich, C5423); H-89 (Beyotime, S1643); cycloheximide (Beyotime, S1560); β 2 -AR siRNA (Santa Cruz, sc-39867); β-arrestin 1 shRNA (Origene, TG510701); β-arrestin 2 shRNA (Origene, TF517061). p27 siRNA (si-P, si-8, and si-10) and control siRNA (siNC) were synthesized by Shanghai Genepharma Co., Ltd. with the following respective sequences: 5' ACGTAAACAGCTCCGAATTAA 3', 5' GGUGCCUUUAAUUGGGUCUTT 3', 5' GGCCAACAGAACAGAAGAATT 3', 5' UUCUCCGAACGUGUCACGUTT 3'.

Techniques: Incubation, Transfection, Expressing, Flow Cytometry