Journal: Molecular Medicine Reports

Article Title: MicroRNA-610 suppresses the proliferation of human glioblastoma cells by repressing CCND2 and AKT3

doi: 10.3892/mmr.2016.4760

Figure Lengend Snippet: miR-610 suppresses CCND2 and AKT3 expression by directly targeting their 3′-UTR, which alters the levels of proteins associated with proliferation in GBM cells. (A) Predicted miR-610 target sequence in the 3′-UTR of CCND2 and AKT3 (CCND2-3′-UTR and AKT3-3′-UTR) and positions of three mutated nucleotides (blue) in the 3′-UTR of miR-610 (miR-610-mut). Luciferase reporter assay of the indicated cells co-transfected with the (B) pGL3-AKT3-3′-UTR reporter and (C) the pGL3-CCND2-3′-UTR reporter and miR-610 or miR-610-in (20 and 50 nM) oligonucleotides. (D) Western blotting analysis of expression of AKT3, CCND2, CCND1, p21, p-pRb and pRb in GBM cells. β-actin served as the loading control. * P<0.05, vs. miR-610+Vector. miR, microRNA; l; GBM, glioblastoma; 3′-UTR, 3′-untranslated region; mut, mutant; CCN, cyclin; pRb, retinoblastoma protein.

Article Snippet: The proteins were then transferred onto polyvinylidene difluoride membranes (EMD Millipore, Billerica, MA, USA), and the membranes were blocked in Tris-buffered saline-Tween 20 (TBST; Beyotime Institute of Biotechnology) supplemented with 5% milk (Beyotime Institute of Biotechnology) for 2 h at room temperature, prior to incubation with the following primary antibodies: Anti-AKT3 (cat. no. 14982), anti-CCND2 (cat. no. 3741) (1:1,000 dilution; Cell Signaling Technology, Inc.), anti-Cyclin D1 (cat. no. 2978; 1:1,000 dilution; Cell Signaling Technology, Inc.), anti-p21 (cat. no. 2947; 1:1,000 dilution; Cell Signaling Technology, Inc.), anti-phosphorylated retinoblastoma protein (p-pRb; cat. no. 8516; 1:1,000 dilution; Cell Signaling Technology, Inc.) and anti-pRb (1:1,000; cat. no. 9313; Cell Signaling Technology, Inc.).

Techniques: Expressing, Sequencing, Luciferase, Reporter Assay, Transfection, Western Blot, Plasmid Preparation, Mutagenesis

Journal: Stem Cell Reviews

Article Title: Acellular Mouse Kidney ECM can be Used as a Three-Dimensional Substrate to Test the Differentiation Potential of Embryonic Stem Cell Derived Renal Progenitors

doi: 10.1007/s12015-016-9712-2

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Article Snippet: Stauprimide , Tocris Biosciences (R&D Systems) , 154589–96-5.

Techniques:

Journal: Cell

Article Title: A global metagenomic map of urban microbiomes and antimicrobial resistance

doi: 10.1016/j.cell.2021.05.002

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Article Snippet: 2D Thermo Scientific Matrix , Thermo Scientific , 3741-WP1D-BR.

Techniques: Environmental Sampling, DNA Extraction, Isolation, Sequencing, Software, Diagnostic Assay, Lysis