3730xl dna analyzer Thermo Fisher Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher dna sequencer
    CaRZFP1 overexpressing transgenic lettuce plants showed hampered growth and development. a Diagrammatic representation of <t>pBKS1-1-</t> CaRZFP1 construct used for lettuce transformation. For the expression vector pBKS1-1, only the region inside of the border sequences, RB and LB, that was actually transferred into the lettuce genome is shown. b RNA blot hybridization results for vector-only and putative T 1 transgenic plant lines. Total RNA was separated by electrophoresis on a 1.2% formaldehyde agarose gel and blotted to a Hybond-N nylon membrane. Separated RNA was stained with ethidium bromide for visualization with UV illumination. The blots were hybridized to 32 P-labeled CaRZFP1 probe. c Typical examples of transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15, and no. 16 and lettuce plants carrying only the vector after 4 weeks since seed imbibition. d Comparison of leaf length of the plants in c . e Comparison of leaf width of the plants in c . f Comparison of fresh weight of the plants in c . g The data in d , e and f were aligned with CaRZFP1 transcript level analyzed by RNA blot hybridization. V10, V30 and V38, lettuce plants carrying only the expression vector. Error bars show standard deviation. VC, average of V10, V30 and V38. h RNA blot hybridization results of vector-only or putative T 3 CaRZFP1 -transgenic lettuce lines that are shown in i . i Typical examples of T 3 transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15 and no. 16 and lettuce plants carrying only the vector after 12 weeks since seed imbibition. j Typical roots of T 3 transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15 and no. 16 and lettuce plants carrying only the vector after 4 weeks since seed imbibition. k Comparison of root mass of the plants in j . l RNA blot hybridization results for the plants in j . m RT-PCR results for the same samples in l . n RT-PCR results in m was further confirmed by <t>DNA</t> blot analysis with 32 P-labeled CaRZFP1 probe. M, size marker. Error bars show standard deviation
    Dna Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna sequencer/product/Thermo Fisher
    Average 99 stars, based on 8308 article reviews
    Price from $9.99 to $1999.99
    dna sequencer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher 3730xl dna analyzer
    Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI <t>3730XL</t> for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).
    3730xl Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 47265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3730xl dna analyzer/product/Thermo Fisher
    Average 99 stars, based on 47265 article reviews
    Price from $9.99 to $1999.99
    3730xl dna analyzer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher dna analyzer
    Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI <t>3730XL</t> for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).
    Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna analyzer/product/Thermo Fisher
    Average 93 stars, based on 1550 article reviews
    Price from $9.99 to $1999.99
    dna analyzer - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    CaRZFP1 overexpressing transgenic lettuce plants showed hampered growth and development. a Diagrammatic representation of pBKS1-1- CaRZFP1 construct used for lettuce transformation. For the expression vector pBKS1-1, only the region inside of the border sequences, RB and LB, that was actually transferred into the lettuce genome is shown. b RNA blot hybridization results for vector-only and putative T 1 transgenic plant lines. Total RNA was separated by electrophoresis on a 1.2% formaldehyde agarose gel and blotted to a Hybond-N nylon membrane. Separated RNA was stained with ethidium bromide for visualization with UV illumination. The blots were hybridized to 32 P-labeled CaRZFP1 probe. c Typical examples of transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15, and no. 16 and lettuce plants carrying only the vector after 4 weeks since seed imbibition. d Comparison of leaf length of the plants in c . e Comparison of leaf width of the plants in c . f Comparison of fresh weight of the plants in c . g The data in d , e and f were aligned with CaRZFP1 transcript level analyzed by RNA blot hybridization. V10, V30 and V38, lettuce plants carrying only the expression vector. Error bars show standard deviation. VC, average of V10, V30 and V38. h RNA blot hybridization results of vector-only or putative T 3 CaRZFP1 -transgenic lettuce lines that are shown in i . i Typical examples of T 3 transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15 and no. 16 and lettuce plants carrying only the vector after 12 weeks since seed imbibition. j Typical roots of T 3 transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15 and no. 16 and lettuce plants carrying only the vector after 4 weeks since seed imbibition. k Comparison of root mass of the plants in j . l RNA blot hybridization results for the plants in j . m RT-PCR results for the same samples in l . n RT-PCR results in m was further confirmed by DNA blot analysis with 32 P-labeled CaRZFP1 probe. M, size marker. Error bars show standard deviation

    Journal: Molecular Breeding

    Article Title: Ectopic RING zinc finger gene from hot pepper induces totally different genes in lettuce and tobacco

    doi: 10.1007/s11032-018-0812-3

    Figure Lengend Snippet: CaRZFP1 overexpressing transgenic lettuce plants showed hampered growth and development. a Diagrammatic representation of pBKS1-1- CaRZFP1 construct used for lettuce transformation. For the expression vector pBKS1-1, only the region inside of the border sequences, RB and LB, that was actually transferred into the lettuce genome is shown. b RNA blot hybridization results for vector-only and putative T 1 transgenic plant lines. Total RNA was separated by electrophoresis on a 1.2% formaldehyde agarose gel and blotted to a Hybond-N nylon membrane. Separated RNA was stained with ethidium bromide for visualization with UV illumination. The blots were hybridized to 32 P-labeled CaRZFP1 probe. c Typical examples of transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15, and no. 16 and lettuce plants carrying only the vector after 4 weeks since seed imbibition. d Comparison of leaf length of the plants in c . e Comparison of leaf width of the plants in c . f Comparison of fresh weight of the plants in c . g The data in d , e and f were aligned with CaRZFP1 transcript level analyzed by RNA blot hybridization. V10, V30 and V38, lettuce plants carrying only the expression vector. Error bars show standard deviation. VC, average of V10, V30 and V38. h RNA blot hybridization results of vector-only or putative T 3 CaRZFP1 -transgenic lettuce lines that are shown in i . i Typical examples of T 3 transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15 and no. 16 and lettuce plants carrying only the vector after 12 weeks since seed imbibition. j Typical roots of T 3 transgenic lettuce plant lines no. 6, no. 12, no. 14, no. 15 and no. 16 and lettuce plants carrying only the vector after 4 weeks since seed imbibition. k Comparison of root mass of the plants in j . l RNA blot hybridization results for the plants in j . m RT-PCR results for the same samples in l . n RT-PCR results in m was further confirmed by DNA blot analysis with 32 P-labeled CaRZFP1 probe. M, size marker. Error bars show standard deviation

    Article Snippet: Nucleotide sequence of the cloned coding region in pBKS1-1 was confirmed by an automated DNA sequencer (3730xI DNA Analyzer, Applied Biosystems).

    Techniques: Transgenic Assay, Construct, Transformation Assay, Expressing, Plasmid Preparation, Northern blot, Hybridization, Electrophoresis, Agarose Gel Electrophoresis, Staining, Labeling, Standard Deviation, Reverse Transcription Polymerase Chain Reaction, Marker

    Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI 3730XL for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).

    Journal: bioRxiv

    Article Title: A deletion in GDF7 is associated with a heritable forebrain commissural malformation concurrent with ventriculomegaly and interhemispheric cysts in cats

    doi: 10.1101/2020.05.12.091686

    Figure Lengend Snippet: Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI 3730XL for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).

    Article Snippet: Sanger sequencing was conducted at the MU DNA Core Facility using an Applied Biosystems 3730xl DNA Analyzer (Applied Biosystems, Foster City, CA, USA) with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems).

    Techniques: Variant Assay, Sequencing, Fluorescence

    Diversity of TCR Vβ repertoire in proliferating T cells. Proliferating T cells were sorted from mouse Rag1 −/− recipients 5 d after lymphocyte T-cell transfer, whereas unfractionated T cells were prepared from C57BL/6 skin-draining lymphocytes. Following isolation of total RNA from proliferating and unfractionated T cells, spectrotype of 22 mouse TCR Vβ gene families was determined by an Applied Biosystems 3730xl DNA Analyzer. ( A ) Total AUC for PCR fragments of individual Vβ gene families relative to positive control. ( B ) Number of peaks for PCR fragments of each TCR Vβ family members ( n = 3, data represents mean ± SEM * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Adaptive immunity to murine skin commensals

    doi: 10.1073/pnas.1401820111

    Figure Lengend Snippet: Diversity of TCR Vβ repertoire in proliferating T cells. Proliferating T cells were sorted from mouse Rag1 −/− recipients 5 d after lymphocyte T-cell transfer, whereas unfractionated T cells were prepared from C57BL/6 skin-draining lymphocytes. Following isolation of total RNA from proliferating and unfractionated T cells, spectrotype of 22 mouse TCR Vβ gene families was determined by an Applied Biosystems 3730xl DNA Analyzer. ( A ) Total AUC for PCR fragments of individual Vβ gene families relative to positive control. ( B ) Number of peaks for PCR fragments of each TCR Vβ family members ( n = 3, data represents mean ± SEM * P

    Article Snippet: Briefly, 22 individual Vβ gene families and controls (both positive and negative) were amplified by cDNA with prelabeled primers and analyzed by Applied Biosystems 3730xl DNA sequencer.

    Techniques: Isolation, Polymerase Chain Reaction, Positive Control