Journal: NPJ Breast Cancer

Article Title: Wnt5a induces ROR1 to recruit cortactin to promote breast-cancer migration and metastasis

doi: 10.1038/s41523-019-0131-9

Figure Lengend Snippet: ROR1 P(841)A fails to associate with cortactin, induce phosphorylation of cortactin, or enhance migration of MCF7 cells. a Schematic depicts the structure of ROR1 protein with different domains. b ΔPRD represents the truncated form of ROR1 without its proline-rich region (PRD). c Amino acid sequences of the PRD of ROR1. Asterisks indicate the proline (P) residues that were replaced with alanine (A). d Immunoblots of anti-ROR1 i.p. by using lysates of MCF7 (Ctrl), MCF7–WT ROR1, or MCF7 cells transfected with ROR1–ΔPRD, as indicated on the top, and probed with antibodies specific for ROR1 or cortactin, as indicated on the left. An immunoblot of the WCL probed with anti-cortactin mAb is in the bottom panel. e Immunoblots of anti-ROR1 i.p. by using lysates of MCF7-Ctrl, MCF7–ΔPRD, MCF7–WT ROR1, or MCF7 transfected with each of the various mutated forms of ROR1, as indicated on the top, and probed with antibodies specific for ROR1, cortactin, or ARHGEF1, as indicated on the left. An immunoblot of the WCL probed with anti-cortactin is in the bottom panel. f Immunoblots of lysates prepared from serum-starved MCF7 (Ctrl), MCF7–ΔPRD, MCF7–WT ROR1, or MCF7 cells transfected with each of the various mutated forms of ROR1, as indicated on top, which subsequently were treated without (−) or with (+) Wnt5a (100 ng/ml), as indicated on the top; the membranes were probed with antibodies specific for ROR1, Cortactin, or pCortactin, as indicated on the left. g Immunoblot of activated RhoA (RhoA–GTP, top panel) or total RhoA (RhoA total, bottom panel) found in lysates of MCF7 (Ctrl), MCF7–ΔPRD, MCF7–WT ROR1, or MCF7 expressing any one of the various mutant forms of ROR1, as indicated on top of each lane in Fig. . h Columns indicate the mean number of migrated cells at 10 h of MCF7 (Ctrl), MCF7–ΔPRD, MCF7 expressing any one of the various mutant forms of ROR1, or MCF7–WT ROR1, as indicated below, which were serum-starved and then examined for migration without (−) or with (+) Wnt5a (200 ng/ml), as indicated at the bottom. Data are shown from three independent experiments ( P < 0.05; P < 0.01, as calculated by one-way ANOVA with Bonferroni’s multiple-comparison test).

Article Snippet: Immunoblot analysis was performed by using primary antibodies specific for cortactin (Cell Signaling Technology, Danvers, MA, USA; dilution 1:1000, catalog#3503), phospho-cortactin (Y421) (Cell Signaling; dilution 1:500, catalog#4569), ARHGEF1 (Cell Signaling; dilution 1:1000, catalog#3669), β-actin (Cell Signaling; dilution 1:1000, catalog#8457), Src (Cell Signaling; dilution 1:1000, catalog#2109), or ROR1 (Cell Signaling; dilution 1:1000, catalog#4102), which were detected with horseradish peroxidase-conjugated secondary antibodies (Cell Signaling).

Techniques: Migration, Western Blot, Transfection, Expressing, Mutagenesis

Journal: Legume Science

Article Title: Recent advances in faba bean genetic and genomic tools for crop improvement

doi: 10.1002/leg3.75

Figure Lengend Snippet: Summary of published transcriptome data in faba bean

Article Snippet: Ocaña et al. ( ) , Transcriptome analysis under ascochyta blight infection , Leaf tissue at 4, 8 and 12 h after inoculation , 21,243 transcripts, 39,060 SNPs and 3669 InDels , Illumina.

Techniques: Sequencing, Genome Wide, Infection

Journal:

Article Title: Analysis of Stable Low-Molecular-Weight RNA Profiles of Members of the Family Rhizobiaceae

doi:

Figure Lengend Snippet: LMW RNA profiles of the reference strains of species belonging to the family Rhizobiaceae. (A) Lane 1, B. japonicum ATCC 10324; lane 2, B. elkanii ATCC 49852; lane 3, B. liaoningense USDA 3622; lane 4, Azorhizobium caulinodans ATCC 43989. (B) Lane 5, Agrobacterium rhizogenes ATCC 13332; lane 6, Agrobacterium radiobacter ATCC 19358; lane 7, Agrobacterium tumefaciens ATCC 23308; lane 8, Agrobacterium rubi ATCC 13335; lane 9, P. myrsinacearum ATCC 43590. (C) Lane 10, S. fredii ATCC 35423; lane 11, S. saheli USDA 4102; lane 12, S. teranga USDA 4101. (D) Lane 13, R. galegae ATCC 43677; lane 14, R. tropici USDA 9030; lane 15, R. etli ATCC 51251; lane 16, R. leguminosarum biovar phaseoli ATCC 14482; lane 17, R. leguminosarum biovar trifolii ATCC 14480; lane 18, R. leguminosarum biovar viceae ATCC 10004; lane 19, R. thiansanense USDA 3592. (E) Lane 20, R. huakuii USDA 4779; lane 21, R. meliloti USDA 1002; lane 22, R. loti ATCC 3669; lane 23, R. ciceri USDA 3383; lane 24, R. mediterraneum USDA 3392. Lanes MW contained molecular weight markers. nt, nucleotides.

Article Snippet: The number of bands present in the tRNA zone was the expected number based on previous results obtained for E. coli CECT99 (= ATCC 9637) with staircase electrophoresis ( 5 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 LMW RNA profiles of the reference strains of species belonging to the family Rhizobiaceae . (A) Lane 1, B. japonicum ATCC 10324; lane 2, B. elkanii ATCC 49852; lane 3, B. liaoningense USDA 3622; lane 4, Azorhizobium caulinodans ATCC 43989. (B) Lane 5, Agrobacterium rhizogenes ATCC 13332; lane 6, Agrobacterium radiobacter ATCC 19358; lane 7, Agrobacterium tumefaciens ATCC 23308; lane 8, Agrobacterium rubi ATCC 13335; lane 9, P. myrsinacearum ATCC 43590. (C) Lane 10, S. fredii ATCC 35423; lane 11, S. saheli USDA 4102; lane 12, S. teranga USDA 4101. (D) Lane 13, R. galegae ATCC 43677; lane 14, R. tropici USDA 9030; lane 15, R. etli ATCC 51251; lane 16, R. leguminosarum biovar phaseoli ATCC 14482; lane 17, R. leguminosarum biovar trifolii ATCC 14480; lane 18, R. leguminosarum biovar viceae ATCC 10004; lane 19, R. thiansanense USDA 3592. (E) Lane 20, R. huakuii USDA 4779; lane 21, R. meliloti USDA 1002; lane 22, R. loti ATCC 3669; lane 23, R. ciceri USDA 3383; lane 24, R. mediterraneum USDA 3392.

Techniques: Molecular Weight