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Image Search Results

Journal: Journal of Cellular and Molecular Medicine
Article Title: Salidroside ameliorates Adriamycin nephropathy in mice by inhibiting β‐catenin activity
doi: 10.1111/jcmm.14340
Figure Lengend Snippet: Activation of β‐catenin by Wnt1 abolishes the protective effects of salidroside against ADR injury in podocytes. (A‐B) The transfection of Wnt1 in cultured podocytes successfully activates β‐catenin activity. (C) Quantitative analysis of Axin2 and Cyclin D1 as shown in (B). (D) The repression of salidroside on β‐catenin activity was counteracted by Wnt1 in ADR‐treated podocytes. (E) Quantitative analysis of Western blots as shown in (D). (F) The inhibition of β‐catenin downstream target gene expressions by salidroside was re‐activated by Wnt1 in ADR‐treated podocytes. (G) Quantitative analysis of Western blots as shown in (F). (H) Activation of β‐catenin by Wnt1 reverses the effects of salidroside on podocin and nephrin expressions in ADR‐injured podocytes. (I) Quantitative analysis of Western blots as shown in (H). (J) Wnt1 has no effects on podocin and nephrin expressions. (K) Quantitative analysis of Western blots as shown in (J). (L) Effects of salidroside on Axin2 and Cyclin D1 expressions in podocytes transfected with the plasmid bearing Wnt1. Lamin A/C and Actin were used as loading controls. The results are the means ± SEM of three independent experiments. EV, empty vector; CON, control; ADR, adriamycin; Sal, salidroside; MMP7, matrix metalloproteinase 7; AGT, angiotensinogen; PAI‐1, plasminogen activator inhibitor‐1. * P < 0.05 and *** P < 0.001 vs CON; # P < 0.05; ## P < 0.01 and ### P < 0.001 vs ADR; ^^ P < 0.01 and ^^^ P < 0.001 vs ADR + Sal
Article Snippet: Cells were transfected with the
Techniques: Activation Assay, Transfection, Cell Culture, Activity Assay, Western Blot, Inhibition, Plasmid Preparation