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  • 93
    ATCC rubrum atcc 35905
    Rubrum Atcc 35905, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rubrum atcc 35905/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rubrum atcc 35905 - by Bioz Stars, 2023-09
    93/100 stars
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    93
    Addgene inc plasmid expressing wnt1
    Activation of β‐catenin by <t>Wnt1</t> abolishes the protective effects of salidroside against ADR injury in podocytes. (A‐B) The transfection of Wnt1 in cultured podocytes successfully activates β‐catenin activity. (C) Quantitative analysis of Axin2 and Cyclin D1 as shown in (B). (D) The repression of salidroside on β‐catenin activity was counteracted by Wnt1 in ADR‐treated podocytes. (E) Quantitative analysis of Western blots as shown in (D). (F) The inhibition of β‐catenin downstream target gene expressions by salidroside was re‐activated by Wnt1 in ADR‐treated podocytes. (G) Quantitative analysis of Western blots as shown in (F). (H) Activation of β‐catenin by Wnt1 reverses the effects of salidroside on podocin and nephrin expressions in ADR‐injured podocytes. (I) Quantitative analysis of Western blots as shown in (H). (J) Wnt1 has no effects on podocin and nephrin expressions. (K) Quantitative analysis of Western blots as shown in (J). (L) Effects of salidroside on Axin2 and Cyclin D1 expressions in podocytes transfected with the plasmid bearing Wnt1. Lamin A/C and Actin were used as loading controls. The results are the means ± SEM of three independent experiments. EV, empty vector; CON, control; ADR, adriamycin; Sal, salidroside; MMP7, matrix metalloproteinase 7; AGT, angiotensinogen; PAI‐1, plasminogen activator inhibitor‐1. * P < 0.05 and *** P < 0.001 vs CON; # P < 0.05; ## P < 0.01 and ### P < 0.001 vs ADR; ^^ P < 0.01 and ^^^ P < 0.001 vs ADR + Sal
    Plasmid Expressing Wnt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid expressing wnt1/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid expressing wnt1 - by Bioz Stars, 2023-09
    93/100 stars
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    86
    Santa Cruz Biotechnology mek2 sirna
    Activation of β‐catenin by <t>Wnt1</t> abolishes the protective effects of salidroside against ADR injury in podocytes. (A‐B) The transfection of Wnt1 in cultured podocytes successfully activates β‐catenin activity. (C) Quantitative analysis of Axin2 and Cyclin D1 as shown in (B). (D) The repression of salidroside on β‐catenin activity was counteracted by Wnt1 in ADR‐treated podocytes. (E) Quantitative analysis of Western blots as shown in (D). (F) The inhibition of β‐catenin downstream target gene expressions by salidroside was re‐activated by Wnt1 in ADR‐treated podocytes. (G) Quantitative analysis of Western blots as shown in (F). (H) Activation of β‐catenin by Wnt1 reverses the effects of salidroside on podocin and nephrin expressions in ADR‐injured podocytes. (I) Quantitative analysis of Western blots as shown in (H). (J) Wnt1 has no effects on podocin and nephrin expressions. (K) Quantitative analysis of Western blots as shown in (J). (L) Effects of salidroside on Axin2 and Cyclin D1 expressions in podocytes transfected with the plasmid bearing Wnt1. Lamin A/C and Actin were used as loading controls. The results are the means ± SEM of three independent experiments. EV, empty vector; CON, control; ADR, adriamycin; Sal, salidroside; MMP7, matrix metalloproteinase 7; AGT, angiotensinogen; PAI‐1, plasminogen activator inhibitor‐1. * P < 0.05 and *** P < 0.001 vs CON; # P < 0.05; ## P < 0.01 and ### P < 0.001 vs ADR; ^^ P < 0.01 and ^^^ P < 0.001 vs ADR + Sal
    Mek2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mek2 sirna/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mek2 sirna - by Bioz Stars, 2023-09
    86/100 stars
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    Image Search Results


    Activation of β‐catenin by Wnt1 abolishes the protective effects of salidroside against ADR injury in podocytes. (A‐B) The transfection of Wnt1 in cultured podocytes successfully activates β‐catenin activity. (C) Quantitative analysis of Axin2 and Cyclin D1 as shown in (B). (D) The repression of salidroside on β‐catenin activity was counteracted by Wnt1 in ADR‐treated podocytes. (E) Quantitative analysis of Western blots as shown in (D). (F) The inhibition of β‐catenin downstream target gene expressions by salidroside was re‐activated by Wnt1 in ADR‐treated podocytes. (G) Quantitative analysis of Western blots as shown in (F). (H) Activation of β‐catenin by Wnt1 reverses the effects of salidroside on podocin and nephrin expressions in ADR‐injured podocytes. (I) Quantitative analysis of Western blots as shown in (H). (J) Wnt1 has no effects on podocin and nephrin expressions. (K) Quantitative analysis of Western blots as shown in (J). (L) Effects of salidroside on Axin2 and Cyclin D1 expressions in podocytes transfected with the plasmid bearing Wnt1. Lamin A/C and Actin were used as loading controls. The results are the means ± SEM of three independent experiments. EV, empty vector; CON, control; ADR, adriamycin; Sal, salidroside; MMP7, matrix metalloproteinase 7; AGT, angiotensinogen; PAI‐1, plasminogen activator inhibitor‐1. * P < 0.05 and *** P < 0.001 vs CON; # P < 0.05; ## P < 0.01 and ### P < 0.001 vs ADR; ^^ P < 0.01 and ^^^ P < 0.001 vs ADR + Sal

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Salidroside ameliorates Adriamycin nephropathy in mice by inhibiting β‐catenin activity

    doi: 10.1111/jcmm.14340

    Figure Lengend Snippet: Activation of β‐catenin by Wnt1 abolishes the protective effects of salidroside against ADR injury in podocytes. (A‐B) The transfection of Wnt1 in cultured podocytes successfully activates β‐catenin activity. (C) Quantitative analysis of Axin2 and Cyclin D1 as shown in (B). (D) The repression of salidroside on β‐catenin activity was counteracted by Wnt1 in ADR‐treated podocytes. (E) Quantitative analysis of Western blots as shown in (D). (F) The inhibition of β‐catenin downstream target gene expressions by salidroside was re‐activated by Wnt1 in ADR‐treated podocytes. (G) Quantitative analysis of Western blots as shown in (F). (H) Activation of β‐catenin by Wnt1 reverses the effects of salidroside on podocin and nephrin expressions in ADR‐injured podocytes. (I) Quantitative analysis of Western blots as shown in (H). (J) Wnt1 has no effects on podocin and nephrin expressions. (K) Quantitative analysis of Western blots as shown in (J). (L) Effects of salidroside on Axin2 and Cyclin D1 expressions in podocytes transfected with the plasmid bearing Wnt1. Lamin A/C and Actin were used as loading controls. The results are the means ± SEM of three independent experiments. EV, empty vector; CON, control; ADR, adriamycin; Sal, salidroside; MMP7, matrix metalloproteinase 7; AGT, angiotensinogen; PAI‐1, plasminogen activator inhibitor‐1. * P < 0.05 and *** P < 0.001 vs CON; # P < 0.05; ## P < 0.01 and ### P < 0.001 vs ADR; ^^ P < 0.01 and ^^^ P < 0.001 vs ADR + Sal

    Article Snippet: Cells were transfected with the plasmid expressing Wnt1 (Addgene, catalog no. #35905) or β‐catenin S33Y (Addgene, catalog no. #19286) using Lipofectamine.

    Techniques: Activation Assay, Transfection, Cell Culture, Activity Assay, Western Blot, Inhibition, Plasmid Preparation