Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Action of Reelin on Oligomerization of ApoER2 and VLDL Receptor in HEK293 Cells Assessed by Time-Resolved Anisotropy and Fluorescence Lifetime Imaging Microscopy

doi: 10.3389/fnmol.2019.00053

Figure Lengend Snippet: The following antibodies were used in this study at the indicated dilutions.

Article Snippet: Phospho-Dab1 (Tyr232) , #3325 , Cell Signaling , WB 1:1,000.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Action of Reelin on Oligomerization of ApoER2 and VLDL Receptor in HEK293 Cells Assessed by Time-Resolved Anisotropy and Fluorescence Lifetime Imaging Microscopy

doi: 10.3389/fnmol.2019.00053

Figure Lengend Snippet: Central fragment of Reelin (R3–6) does not induce Dab1 phosphorylation. HEK293 cells expressing Dab1_myc and ApoER2 (A) or Dab1_myc and VLDLR (B) or Dab1_myc, ApoER2, and VLDLR (C) were kept in Imaging Medium for 30 min. Next, the medium was changed to RCM containing full length Reelin (FL Rln, lanes 2) or R3–6 (lanes 3) was added to the cells (final concentration, 30 nM) or cells were left untreated (neg.ctrl, lanes 1). After 20 min incubation at 37°C, cells were washed and lysed in NP-40 lysis buffer. Lysates were analyzed by western blotting using phospho-Dab1 antibody (Ab pDab1) and GAPDH antibody. Membranes were stripped and subsequently re-probed for the detection of Dab1 (AbD4). ApoER2 was detected by Ab20 and VLDLR by AbAF2258.

Article Snippet: Phospho-Dab1 (Tyr232) , #3325 , Cell Signaling , WB 1:1,000.

Techniques: Expressing, Imaging, Concentration Assay, Incubation, Lysis, Western Blot

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Action of Reelin on Oligomerization of ApoER2 and VLDL Receptor in HEK293 Cells Assessed by Time-Resolved Anisotropy and Fluorescence Lifetime Imaging Microscopy

doi: 10.3389/fnmol.2019.00053

Figure Lengend Snippet: Model of ApoER2 and VLDLR mediated signaling during cortical development. Radial glia cells divide asymmetrically to produce postmitotic neurons. During early stages of cortical development these neurons migrate via somal translocation. When the thickness of the cerebral wall increases neurons switch their migration mode to multipolar migration followed by locomotion. Thus, polarized neurons leave the ventricular (VZ) and subventricular zone (SVZ), depolarize and migrate through the IZ via a multipolar migration mode. The depolarization step is ill defined and may involve ApoER2 (A)-mediated signaling driven by the central Reelin fragment R3–6 (box 1). Repolarization of the neurons as prerequisite to glia cell guided locomotion is induced by Dab1 phosphorylation mediated by a netrin 1—deleted in colon cancer (DCC) signaling cascade and/or by the canonical Reelin pathway (box 2). Locomotion and terminal translocation is orchestrated by a complex set of Reelin (full length, FL) signals transmitted by clustering of ApoER2 (A) and VLDLR (V) homo- oligomers, leading to Dab1 phosphorylation (box 3). CP, cortical plate; IZ, intermediate zone; MZ, marginal zone.

Article Snippet: Phospho-Dab1 (Tyr232) , #3325 , Cell Signaling , WB 1:1,000.

Techniques: Translocation Assay, Migration

Journal: Cell Reports

Article Title: p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation

doi: 10.1016/j.celrep.2021.109153

Figure Lengend Snippet:

Article Snippet: Hoechst 3325 , Sigma-Aldrich , Cat# 23491-45-4.

Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Sequencing, Modification, Transfection, Imaging, Magnetic Beads, Software