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Article Title: Cloning and Characterization of a Gene ( mspA ) Encoding the Major Sheath Protein of Treponema maltophilum ATCC 51939 T

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Figure Lengend Snippet: Distribution of mspA homologs in various Treponema strains

Article Snippet: All bacterial strains used in this study are listed in Table and were maintained as described previously ( 30 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain Phylogenetic group a Reaction with anti-OMF antibodies b Hybridization of DNA with: mspA probe c msp probe d T. vincentii RitzA I − − +/− e T. phagedenis biotype Reiter − − − T. denticola ATCC 33521 − − + CD-1 II − − + ATCC 35405 − − + T. maltophilum ATCC 51939 T + + − Treponema clinical isolate I f IV +/− +/− − Treponema clinical isolate II f + + − T. brennaborense DSM 12168 T + +/− − T. amylovorum ATCC 700288 T − ND g ND T. socranskii subsp. socranskii ATCC 35536 T +/− +/− − T. socranskii subsp. buccale ATCC 35534 T +/− +/− − T. pectinovorum ATCC 33768 T − − − Open in a separate window a For phylogenetic group determination, see reference . b Determined by Western blot analysis of whole-cell extracts of the bacteria. c Internal T. maltophilum mspA -specific Hin dIII DNA fragment (see Fig. ). d PCR-generated T. denticola ATCC 33521 msp -specific DNA restriction fragment. e +/−, weak positive reaction. f Clinical isolates I and II were kindly provided by C. Wyss, Institute for Oral Microbiology and Immunology, Center for Dentistry, University of Zürich, Zurich, Switzerland. g ND, not determined.

Techniques: Hybridization