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  • 94
    Cell Signaling Technology Inc phospho ros1
    Phospho Ros1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ros1/product/Cell Signaling Technology Inc
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    90
    Santa Cruz Biotechnology anti dkk1
    Left panel: Western blot analysis of <t>DKK1</t> in 13.5 dpc embryonic male gonad, mesonephros, and tongue. As DKK1 western blot negative control SKOV3 cell lysates are also reported. One band, at 35 KDa molecular weight was detected. Right panel: Confocal microscopy analysis of DKK1 distribution, observed by whole mount immunofluorescence (FITC signal) in 11.5 (A), 12.5 (C and D), 13.5 (E and F), 15.5 (G and H), and 18.5 dpc (I and L) male UGRs at different magnifications. In B the corresponding bright field of 11.5 dpc male genital ridge is reported. In M the DKK1 signal in the tongue of 13.5 dpc embryos is reported as positive control. In N the corresponding bright field has been shown. gr: genital ridge; ms: mesonephros; tc: testicular cords; ts: testis.
    Anti Dkk1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti dkk1/product/Santa Cruz Biotechnology
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    85
    Thermo Fisher pri mirna mmu mir 3078 mm03307218 pri
    Analysis of kinetics of expression of the miRNA-124 precursor transcripts <t> pri-miRNA-124.1, pri-miRNA-124.2 </t> and pri-miRNA-124.3 in RAW264.7 cells treated with IL-4 <xref ref-type= 1 ." width="250" height="auto" />
    Pri Mirna Mmu Mir 3078 Mm03307218 Pri, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pri mirna mmu mir 3078 mm03307218 pri/product/Thermo Fisher
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    86
    DuPont de Nemours hytrel 3078
    Analysis of kinetics of expression of the miRNA-124 precursor transcripts <t> pri-miRNA-124.1, pri-miRNA-124.2 </t> and pri-miRNA-124.3 in RAW264.7 cells treated with IL-4 <xref ref-type= 1 ." width="250" height="auto" />
    Hytrel 3078, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Left panel: Western blot analysis of DKK1 in 13.5 dpc embryonic male gonad, mesonephros, and tongue. As DKK1 western blot negative control SKOV3 cell lysates are also reported. One band, at 35 KDa molecular weight was detected. Right panel: Confocal microscopy analysis of DKK1 distribution, observed by whole mount immunofluorescence (FITC signal) in 11.5 (A), 12.5 (C and D), 13.5 (E and F), 15.5 (G and H), and 18.5 dpc (I and L) male UGRs at different magnifications. In B the corresponding bright field of 11.5 dpc male genital ridge is reported. In M the DKK1 signal in the tongue of 13.5 dpc embryos is reported as positive control. In N the corresponding bright field has been shown. gr: genital ridge; ms: mesonephros; tc: testicular cords; ts: testis.

    Journal: PLoS ONE

    Article Title: R-Spondin 1/Dickkopf-1/Beta-Catenin Machinery Is Involved in Testicular Embryonic Angiogenesis

    doi: 10.1371/journal.pone.0124213

    Figure Lengend Snippet: Left panel: Western blot analysis of DKK1 in 13.5 dpc embryonic male gonad, mesonephros, and tongue. As DKK1 western blot negative control SKOV3 cell lysates are also reported. One band, at 35 KDa molecular weight was detected. Right panel: Confocal microscopy analysis of DKK1 distribution, observed by whole mount immunofluorescence (FITC signal) in 11.5 (A), 12.5 (C and D), 13.5 (E and F), 15.5 (G and H), and 18.5 dpc (I and L) male UGRs at different magnifications. In B the corresponding bright field of 11.5 dpc male genital ridge is reported. In M the DKK1 signal in the tongue of 13.5 dpc embryos is reported as positive control. In N the corresponding bright field has been shown. gr: genital ridge; ms: mesonephros; tc: testicular cords; ts: testis.

    Article Snippet: After quenching, membranes were incubated with anti-RSPO1 (1:1000 dilution, goat polyclonal, AF3474, R&D System, Minneapolis, Minnesota, USA), anti-DKK1 (1:200 dilution, goat polyclonal, sc-30785, Santa Cruz Biotechnology, Heidelberg, Germany, Europe), or anti-α-tubulin (1:1000 dilution, mouse monoclonal, T5168, Sigma-Aldrich, St. Louis, Missouri, USA).

    Techniques: Western Blot, Negative Control, Molecular Weight, Confocal Microscopy, Immunofluorescence, Positive Control

    Confocal microscopy analysis of PECAM1 localization, observed by whole mount immunofluorescence in 12.5 dpc male gonads cultured for 48 hours in medium alone (A), in the presence of DKK1 at concentration of 1 μg/ml (C), 2 μg/ml (E) and 4 μg/ml (G) and in the presence of DKK1/RSPO1 at concentration of 2 and 3 μg/ml respectively (I). The corresponding TO-PRO3 staining (nuclei) are reported in B,D,F,H, and L. White arrowheads indicate the coelomic vessel. ms: mesonephros; ts: testis.

    Journal: PLoS ONE

    Article Title: R-Spondin 1/Dickkopf-1/Beta-Catenin Machinery Is Involved in Testicular Embryonic Angiogenesis

    doi: 10.1371/journal.pone.0124213

    Figure Lengend Snippet: Confocal microscopy analysis of PECAM1 localization, observed by whole mount immunofluorescence in 12.5 dpc male gonads cultured for 48 hours in medium alone (A), in the presence of DKK1 at concentration of 1 μg/ml (C), 2 μg/ml (E) and 4 μg/ml (G) and in the presence of DKK1/RSPO1 at concentration of 2 and 3 μg/ml respectively (I). The corresponding TO-PRO3 staining (nuclei) are reported in B,D,F,H, and L. White arrowheads indicate the coelomic vessel. ms: mesonephros; ts: testis.

    Article Snippet: After quenching, membranes were incubated with anti-RSPO1 (1:1000 dilution, goat polyclonal, AF3474, R&D System, Minneapolis, Minnesota, USA), anti-DKK1 (1:200 dilution, goat polyclonal, sc-30785, Santa Cruz Biotechnology, Heidelberg, Germany, Europe), or anti-α-tubulin (1:1000 dilution, mouse monoclonal, T5168, Sigma-Aldrich, St. Louis, Missouri, USA).

    Techniques: Confocal Microscopy, Immunofluorescence, Cell Culture, Concentration Assay, Staining

    In the panel A, a graph indicating the number of pHistone H3 positive cells/μm 3 observed in testes cultured for 48 hours in medium alone (control) and in presence of 2 μg/ml DKK1 is reported. Statistical analysis, evaluated by Student's T test, was not significant. A representative merging optical section of pHistone H3 signal (FITC) and TO-PRO3 staining (nuclei) is reported in the right part of the panel. In the panel B, a graph indicating the quantitative analysis of Ki67 fluorescence intensity observed in testes cultured for 48 hours in medium alone (control) and in presence of 4 μg/ml DKK1 is reported in (I). Statistical analysis, evaluated by Student's T test, was not significant. A representative merging optical section of Ki67 signal (TRITC) and TO-PRO3 staining (nuclei) is reported in the right part of the panel. In (II) a graph indicating the number of Ki67 positive germ cells per testis is reported (Ki67/PECAM1 double positive cells inside the testicular cords). In (III) a graph indicating the number of Ki67 positive endothelial cells per testis is reported (Ki67/PECAM1 double positive cells in the interstitial compartment). A representative merging optical section of Ki67 signal (TRITC) and PECAM1 staining (FITC) is reported in the right part of the panels II e III. The white arrowhead indicates endothelial cells and the white arrow indicates germ cells. In the panel C the graph indicates the number of Caspase-3 positive cells/μm 3 observed in testes cultured for 48 hours in medium alone (control) and in presence of 4 μg/ml DKK1. Statistical analysis, evaluated by Student's T test, was not significant. In the right part of the panel a representative optical section merging Caspase-3 (TRITC) and TO-PRO3 (nuclei) staining is reported.

    Journal: PLoS ONE

    Article Title: R-Spondin 1/Dickkopf-1/Beta-Catenin Machinery Is Involved in Testicular Embryonic Angiogenesis

    doi: 10.1371/journal.pone.0124213

    Figure Lengend Snippet: In the panel A, a graph indicating the number of pHistone H3 positive cells/μm 3 observed in testes cultured for 48 hours in medium alone (control) and in presence of 2 μg/ml DKK1 is reported. Statistical analysis, evaluated by Student's T test, was not significant. A representative merging optical section of pHistone H3 signal (FITC) and TO-PRO3 staining (nuclei) is reported in the right part of the panel. In the panel B, a graph indicating the quantitative analysis of Ki67 fluorescence intensity observed in testes cultured for 48 hours in medium alone (control) and in presence of 4 μg/ml DKK1 is reported in (I). Statistical analysis, evaluated by Student's T test, was not significant. A representative merging optical section of Ki67 signal (TRITC) and TO-PRO3 staining (nuclei) is reported in the right part of the panel. In (II) a graph indicating the number of Ki67 positive germ cells per testis is reported (Ki67/PECAM1 double positive cells inside the testicular cords). In (III) a graph indicating the number of Ki67 positive endothelial cells per testis is reported (Ki67/PECAM1 double positive cells in the interstitial compartment). A representative merging optical section of Ki67 signal (TRITC) and PECAM1 staining (FITC) is reported in the right part of the panels II e III. The white arrowhead indicates endothelial cells and the white arrow indicates germ cells. In the panel C the graph indicates the number of Caspase-3 positive cells/μm 3 observed in testes cultured for 48 hours in medium alone (control) and in presence of 4 μg/ml DKK1. Statistical analysis, evaluated by Student's T test, was not significant. In the right part of the panel a representative optical section merging Caspase-3 (TRITC) and TO-PRO3 (nuclei) staining is reported.

    Article Snippet: After quenching, membranes were incubated with anti-RSPO1 (1:1000 dilution, goat polyclonal, AF3474, R&D System, Minneapolis, Minnesota, USA), anti-DKK1 (1:200 dilution, goat polyclonal, sc-30785, Santa Cruz Biotechnology, Heidelberg, Germany, Europe), or anti-α-tubulin (1:1000 dilution, mouse monoclonal, T5168, Sigma-Aldrich, St. Louis, Missouri, USA).

    Techniques: Cell Culture, Staining, Fluorescence

    Real-time PCR analysis of Pecam1 (A), Fgf9 (B), Pdgf-b (C), and Vegfa (D) in control (light gray columns) and DKK1 treated samples (dark gray columns); the number depicted above the graphs (when reported) means the fold change of transcript expression in treated samples vs. control samples. Error bars indicate s.e.m. (*P < 0.05; ** P < 0.01).

    Journal: PLoS ONE

    Article Title: R-Spondin 1/Dickkopf-1/Beta-Catenin Machinery Is Involved in Testicular Embryonic Angiogenesis

    doi: 10.1371/journal.pone.0124213

    Figure Lengend Snippet: Real-time PCR analysis of Pecam1 (A), Fgf9 (B), Pdgf-b (C), and Vegfa (D) in control (light gray columns) and DKK1 treated samples (dark gray columns); the number depicted above the graphs (when reported) means the fold change of transcript expression in treated samples vs. control samples. Error bars indicate s.e.m. (*P < 0.05; ** P < 0.01).

    Article Snippet: After quenching, membranes were incubated with anti-RSPO1 (1:1000 dilution, goat polyclonal, AF3474, R&D System, Minneapolis, Minnesota, USA), anti-DKK1 (1:200 dilution, goat polyclonal, sc-30785, Santa Cruz Biotechnology, Heidelberg, Germany, Europe), or anti-α-tubulin (1:1000 dilution, mouse monoclonal, T5168, Sigma-Aldrich, St. Louis, Missouri, USA).

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Analysis of kinetics of expression of the miRNA-124 precursor transcripts  pri-miRNA-124.1, pri-miRNA-124.2  and pri-miRNA-124.3 in RAW264.7 cells treated with IL-4 <xref ref-type= 1 ." width="100%" height="100%">

    Journal: PLoS ONE

    Article Title: IL-4/IL-13-Dependent and Independent Expression of miR-124 and Its Contribution to M2 Phenotype of Monocytic Cells in Normal Conditions and during Allergic Inflammation

    doi: 10.1371/journal.pone.0081774

    Figure Lengend Snippet: Analysis of kinetics of expression of the miRNA-124 precursor transcripts pri-miRNA-124.1, pri-miRNA-124.2 and pri-miRNA-124.3 in RAW264.7 cells treated with IL-4 1 .

    Article Snippet: Pri-miR124.1, pri-miR-124.2 and pri-miR-124.3 were analyzed using the mm03307218 (pri-mmu-124-1), mm03307226 (pri-mmu-124-2), and mm03306225 (pri-mmu-124-3) primers from Life Technologies.

    Techniques: Expressing

    − ), intermediate (CD14 + CD16 + ) and non-classical (CD14 low CD16 + ) human monocytes. ( A ) Mononuclear cells were isolated from the peripheral blood of healthy individuals and stained for CD14, CD16 and CD4. The populations of CD14 ++ CD16 − , CD14 + CD16 + , and CD14 low CD16 + monocytes and CD14-CD16-CD4+ CD4 subsets were sorted by FACS and the expression of miR-124, miR-155, and miR-424 was analyzed as described in Materials and Methods . The mean MFI ± S.E. of three separate experiments with three healthy individuals is shown. Expression of miR-124 ( B ), miR-155 ( C ) and miR-424 ( D ) was analyzed in three sorted monocyte populations and compared to CD4 T cells. The level of expression of indicated miRNAs in CD4 T cells was used as a reference value.

    Journal: PLoS ONE

    Article Title: IL-4/IL-13-Dependent and Independent Expression of miR-124 and Its Contribution to M2 Phenotype of Monocytic Cells in Normal Conditions and during Allergic Inflammation

    doi: 10.1371/journal.pone.0081774

    Figure Lengend Snippet: − ), intermediate (CD14 + CD16 + ) and non-classical (CD14 low CD16 + ) human monocytes. ( A ) Mononuclear cells were isolated from the peripheral blood of healthy individuals and stained for CD14, CD16 and CD4. The populations of CD14 ++ CD16 − , CD14 + CD16 + , and CD14 low CD16 + monocytes and CD14-CD16-CD4+ CD4 subsets were sorted by FACS and the expression of miR-124, miR-155, and miR-424 was analyzed as described in Materials and Methods . The mean MFI ± S.E. of three separate experiments with three healthy individuals is shown. Expression of miR-124 ( B ), miR-155 ( C ) and miR-424 ( D ) was analyzed in three sorted monocyte populations and compared to CD4 T cells. The level of expression of indicated miRNAs in CD4 T cells was used as a reference value.

    Article Snippet: Pri-miR124.1, pri-miR-124.2 and pri-miR-124.3 were analyzed using the mm03307218 (pri-mmu-124-1), mm03307226 (pri-mmu-124-2), and mm03306225 (pri-mmu-124-3) primers from Life Technologies.

    Techniques: Isolation, Staining, Expressing