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    Fold changes of proteins in primary cortical cells upon HSV-1 infection.
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    Fold changes of proteins in primary cortical cells upon HSV-1 infection.
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    Fold changes of proteins in primary cortical cells upon HSV-1 infection.
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    Fold changes of proteins in primary cortical cells upon HSV-1 infection.

    Journal: Frontiers in Neurology

    Article Title: The Proteome and Secretome of Cortical Brain Cells Infected With Herpes Simplex Virus

    doi: 10.3389/fneur.2020.00844

    Figure Lengend Snippet: Fold changes of proteins in primary cortical cells upon HSV-1 infection.

    Article Snippet: After blotting on nitrocellulose membranes (GE Healthcare), following antibodies were used: self-raised polyclonal rabbit anticapsid antibody HSV-1 , Nek7 (Cell Signaling, monoclonal rabbit, #3057), and histone H3 (Cell Signaling, polyclonal rabbit, #9715).

    Techniques: Infection

    Nek7 Western blots of HSV-1 infected primary cortical cells. Primary cortical cells were infected at DIV6 with HSV-1(17+)LoxGFP at a MOI = 10 and analyzed 8 hpi. (A) Representative Western blot of mock infected control (ctrl.) cells and HSV-1 infected cells developed with a Nek7, HSV-1, and histone H3 antibody. (B) Densitometric analysis of Nek7 expression normalized by histone H3 expression. Bar graph displays mean + SEM and individual datapoints. n = 4, Student's t-test with ** p < 0.01.

    Journal: Frontiers in Neurology

    Article Title: The Proteome and Secretome of Cortical Brain Cells Infected With Herpes Simplex Virus

    doi: 10.3389/fneur.2020.00844

    Figure Lengend Snippet: Nek7 Western blots of HSV-1 infected primary cortical cells. Primary cortical cells were infected at DIV6 with HSV-1(17+)LoxGFP at a MOI = 10 and analyzed 8 hpi. (A) Representative Western blot of mock infected control (ctrl.) cells and HSV-1 infected cells developed with a Nek7, HSV-1, and histone H3 antibody. (B) Densitometric analysis of Nek7 expression normalized by histone H3 expression. Bar graph displays mean + SEM and individual datapoints. n = 4, Student's t-test with ** p < 0.01.

    Article Snippet: After blotting on nitrocellulose membranes (GE Healthcare), following antibodies were used: self-raised polyclonal rabbit anticapsid antibody HSV-1 , Nek7 (Cell Signaling, monoclonal rabbit, #3057), and histone H3 (Cell Signaling, polyclonal rabbit, #9715).

    Techniques: Western Blot, Infection, Expressing