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    DSMZ micrococcus luteus
    Activity of C. cinerea GH24-type lysozymes on Gram-positive bacteria. a – f Bacterial cell lysis activity was determined in a microtiter plate turbidity assay for B. subtilis , M. <t>luteus</t> , S. carnosus and S. aureus . Cells were treated with the following proteins at a concentration of 20 µg/ml: Untagged recombinant lysozymes (LYS1-3), His6-tagged LYS2 (LYS2His), His6-tagged lysozyme domain of LYS2 (LYS2His∆N), His6-tagged catalytic mutant LYS2 (LYS2(D131A)His), hen egg white lysozyme (HEWL) as positive and bovine serum albumin (BSA) as negative control. The bacterial suspensions were incubated at 37 °C and data points were acquired at an optical density of 450 nm in 5 min time intervals. The average of three biological replicates is shown together with the standard deviation. g Lysozyme activity was determined using the EnzChek Lysozyme assay kit (Thermo Scientific). 4-fold dilution series of the proteins listed above were incubated with M. luteus fluorescein-labeled cell wall. The release of fluorescein due to hydrolysis of cell wall was measured after 30 min of incubation at 37 °C with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Each data point represents the average of three replicates. Error bars indicate standard deviation
    Micrococcus Luteus, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micrococcus luteus/product/DSMZ
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    micrococcus luteus - by Bioz Stars, 2024-02
    93/100 stars
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    Activity of C. cinerea GH24-type lysozymes on Gram-positive bacteria. a – f Bacterial cell lysis activity was determined in a microtiter plate turbidity assay for B. subtilis , M. luteus , S. carnosus and S. aureus . Cells were treated with the following proteins at a concentration of 20 µg/ml: Untagged recombinant lysozymes (LYS1-3), His6-tagged LYS2 (LYS2His), His6-tagged lysozyme domain of LYS2 (LYS2His∆N), His6-tagged catalytic mutant LYS2 (LYS2(D131A)His), hen egg white lysozyme (HEWL) as positive and bovine serum albumin (BSA) as negative control. The bacterial suspensions were incubated at 37 °C and data points were acquired at an optical density of 450 nm in 5 min time intervals. The average of three biological replicates is shown together with the standard deviation. g Lysozyme activity was determined using the EnzChek Lysozyme assay kit (Thermo Scientific). 4-fold dilution series of the proteins listed above were incubated with M. luteus fluorescein-labeled cell wall. The release of fluorescein due to hydrolysis of cell wall was measured after 30 min of incubation at 37 °C with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Each data point represents the average of three replicates. Error bars indicate standard deviation

    Journal: The ISME Journal

    Article Title: Induction of antibacterial proteins and peptides in the coprophilous mushroom Coprinopsis cinerea in response to bacteria

    doi: 10.1038/s41396-018-0293-8

    Figure Lengend Snippet: Activity of C. cinerea GH24-type lysozymes on Gram-positive bacteria. a – f Bacterial cell lysis activity was determined in a microtiter plate turbidity assay for B. subtilis , M. luteus , S. carnosus and S. aureus . Cells were treated with the following proteins at a concentration of 20 µg/ml: Untagged recombinant lysozymes (LYS1-3), His6-tagged LYS2 (LYS2His), His6-tagged lysozyme domain of LYS2 (LYS2His∆N), His6-tagged catalytic mutant LYS2 (LYS2(D131A)His), hen egg white lysozyme (HEWL) as positive and bovine serum albumin (BSA) as negative control. The bacterial suspensions were incubated at 37 °C and data points were acquired at an optical density of 450 nm in 5 min time intervals. The average of three biological replicates is shown together with the standard deviation. g Lysozyme activity was determined using the EnzChek Lysozyme assay kit (Thermo Scientific). 4-fold dilution series of the proteins listed above were incubated with M. luteus fluorescein-labeled cell wall. The release of fluorescein due to hydrolysis of cell wall was measured after 30 min of incubation at 37 °C with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Each data point represents the average of three replicates. Error bars indicate standard deviation

    Article Snippet: Micrococcus luteus (DSMZ 20030) was cultivated in nutrient broth (Difco, BD Diagnostics) at 28 °C to an OD 600 of 2.

    Techniques: Activity Assay, Lysis, Concentration Assay, Recombinant, Mutagenesis, Negative Control, Incubation, Standard Deviation, Labeling