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    CD MAT Has a Distinct Cultivable Microbiota Dominated by Clostridium innocuum (A) Key cultivable organisms recovered from CD, UC, and H MAT. Bacteria found in more than one specimen are shown. Each column represents the cultivable community for an individual patient. Organisms recovered solely from CD, UC, or H are shaded black. (B) Venn diagram denoting number of unique bacterial species identified by 16S rRNA sequencing and by cultivation methods. (C) Compositionally coherent log-ratio t tests of metagenomic sequences from the five bacteria exclusively cultivated in CD samples ( C. innocuum , E. ramosum , P. distasonis , C. symbiosum , and B. pseudolongum ; <xref ref-type=Figure 3 A) to an H-MAT-exclusive bacteria, P. merdae , identified by Songbird multinomial regression. (D) Whole genome sequencing comparison of C. innocuum isolates recovered from CD CrF (n = 11), CD MAT (n = 6), CD mucosa (n = 8), and UC mucosa (n = 5), as well as a reference genome, C. innocuum 2959 and type strain DSM1286. Disease and tissue distribution of samples are coded on the right. (E) Differentially abundant KEGG pathways across C. innocuum isolates. R = reference strains. (F) Functional phenotyping of C. innocuum isolates by Biolog in vitro substrate utilization assay. Growth of each isolate was screened against 95 different substrates as the sole nutrient source. R = DSM1286 type strain. Student’s t tests were performed for (C) given a priori knowledge of CD and H-MAT-associated bacteria. Kruskal-Wallis test was performed for (E). See also Figure S3 and and " width="100%" height="100%">

    Journal: Cell

    Article Title: Translocation of Viable Gut Microbiota to Mesenteric Adipose Drives Formation of Creeping Fat in Humans

    doi: 10.1016/j.cell.2020.09.009

    Figure Lengend Snippet: CD MAT Has a Distinct Cultivable Microbiota Dominated by Clostridium innocuum (A) Key cultivable organisms recovered from CD, UC, and H MAT. Bacteria found in more than one specimen are shown. Each column represents the cultivable community for an individual patient. Organisms recovered solely from CD, UC, or H are shaded black. (B) Venn diagram denoting number of unique bacterial species identified by 16S rRNA sequencing and by cultivation methods. (C) Compositionally coherent log-ratio t tests of metagenomic sequences from the five bacteria exclusively cultivated in CD samples ( C. innocuum , E. ramosum , P. distasonis , C. symbiosum , and B. pseudolongum ; Figure 3 A) to an H-MAT-exclusive bacteria, P. merdae , identified by Songbird multinomial regression. (D) Whole genome sequencing comparison of C. innocuum isolates recovered from CD CrF (n = 11), CD MAT (n = 6), CD mucosa (n = 8), and UC mucosa (n = 5), as well as a reference genome, C. innocuum 2959 and type strain DSM1286. Disease and tissue distribution of samples are coded on the right. (E) Differentially abundant KEGG pathways across C. innocuum isolates. R = reference strains. (F) Functional phenotyping of C. innocuum isolates by Biolog in vitro substrate utilization assay. Growth of each isolate was screened against 95 different substrates as the sole nutrient source. R = DSM1286 type strain. Student’s t tests were performed for (C) given a priori knowledge of CD and H-MAT-associated bacteria. Kruskal-Wallis test was performed for (E). See also Figure S3 and and

    Article Snippet: Clostridium innocuum strain DSM1286 , DSMZ , Strain: DSM1286.

    Techniques: Sequencing, Functional Assay, In Vitro

    Journal: Cell

    Article Title: Translocation of Viable Gut Microbiota to Mesenteric Adipose Drives Formation of Creeping Fat in Humans

    doi: 10.1016/j.cell.2020.09.009

    Figure Lengend Snippet:

    Article Snippet: Clostridium innocuum strain DSM1286 , DSMZ , Strain: DSM1286.

    Techniques: Recombinant, Preserving, Lysis, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, RNA Sequencing Assay, Software