3-methyl phenol Search Results


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  • 99
    Thermo Fisher 3 methyl 1 butanol
    Treatment with a TRPV agonist dysregulates chemotaxis of B . pahangi infective larvae. (A) L3 parasites were extracted from mosquitoes and subjected to chemotaxis assays with or without 250 μM NAM treatment. Chemotaxis assays were performed by adding L3s to the middle of a 0.8% agarose plate (M), with either test cue (T) or water (C) added to the opposite sides of the plate. The plate was placed at 37°C for 30 minutes, scored after incubation, and the CI was calculated. (B) NAM dysregulates attraction of freshly extracted L3s to serum and NaCl but has no effect on aversion to <t>3-methyl-1-butanol.</t> (C) NAM has no effect on translational movement of freshly extracted L3s. (D) Bpa-osm-9 expression is unchanged by in vitro culture at physiological or room temperature 4 HPE. (E) L3s cultured for 1 DPE do not show chemotaxis toward serum and have reduced motility on the chemotaxis plate when compared with untreated freshly extracted parasites ( p = 0.028, t test). Data for (A–C) represent the combined results of three independent biological replicates, except for the experiments with 3-methyl-1-butanol, which included two replicates (cohorts of mosquito infections). Data for (E) represent the results of two biological replicates. Each point represents a single chemotaxis plate with 8–10 L3s. Red diamonds and bars indicate the mean and standard error of the mean. Comparisons of means were performed using t tests (** p ≤ 0.01). Raw data for (B) through (E) can be found at https://github.com/zamanianlab/BrugiaChemo-ms . CI, chemotaxis index; DPE, days postextraction; FBS, fetal bovine serum; HPE, hours postextraction; L3, third stage larvae; NAM, nicotinamide; ns, not significant; TRP, transient receptor potential.
    3 Methyl 1 Butanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore 3 methylphenol
    Treatment with a TRPV agonist dysregulates chemotaxis of B . pahangi infective larvae. (A) L3 parasites were extracted from mosquitoes and subjected to chemotaxis assays with or without 250 μM NAM treatment. Chemotaxis assays were performed by adding L3s to the middle of a 0.8% agarose plate (M), with either test cue (T) or water (C) added to the opposite sides of the plate. The plate was placed at 37°C for 30 minutes, scored after incubation, and the CI was calculated. (B) NAM dysregulates attraction of freshly extracted L3s to serum and NaCl but has no effect on aversion to <t>3-methyl-1-butanol.</t> (C) NAM has no effect on translational movement of freshly extracted L3s. (D) Bpa-osm-9 expression is unchanged by in vitro culture at physiological or room temperature 4 HPE. (E) L3s cultured for 1 DPE do not show chemotaxis toward serum and have reduced motility on the chemotaxis plate when compared with untreated freshly extracted parasites ( p = 0.028, t test). Data for (A–C) represent the combined results of three independent biological replicates, except for the experiments with 3-methyl-1-butanol, which included two replicates (cohorts of mosquito infections). Data for (E) represent the results of two biological replicates. Each point represents a single chemotaxis plate with 8–10 L3s. Red diamonds and bars indicate the mean and standard error of the mean. Comparisons of means were performed using t tests (** p ≤ 0.01). Raw data for (B) through (E) can be found at https://github.com/zamanianlab/BrugiaChemo-ms . CI, chemotaxis index; DPE, days postextraction; FBS, fetal bovine serum; HPE, hours postextraction; L3, third stage larvae; NAM, nicotinamide; ns, not significant; TRP, transient receptor potential.
    3 Methylphenol, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore 4 chloro 3 methylphenol
    : Concentration-response curves in SIN-1 treated (70 min) and untreated (control) cardiomyocytes for [Ca 2+ ] i increase upon stimulation with (A) ATP (agonist of L-type voltage dependent Ca 2+ channels), (B) palmitoyl-oleoyl-phosphatidic acid (POPA; agonist of inositol 1,4,5-trisphosphate receptors) and (C) <t>4-chloro-3-methylphenol</t> (agonist of ryanodine receptor 2). Data is shown as means±SE. Results are representatives of three independent experiments performed in triplicates.
    4 Chloro 3 Methylphenol, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore 3 methyl 4 methylthio phenol
    : Concentration-response curves in SIN-1 treated (70 min) and untreated (control) cardiomyocytes for [Ca 2+ ] i increase upon stimulation with (A) ATP (agonist of L-type voltage dependent Ca 2+ channels), (B) palmitoyl-oleoyl-phosphatidic acid (POPA; agonist of inositol 1,4,5-trisphosphate receptors) and (C) <t>4-chloro-3-methylphenol</t> (agonist of ryanodine receptor 2). Data is shown as means±SE. Results are representatives of three independent experiments performed in triplicates.
    3 Methyl 4 Methylthio Phenol, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 4 chloro 3 methyl phenol
    : Concentration-response curves in SIN-1 treated (70 min) and untreated (control) cardiomyocytes for [Ca 2+ ] i increase upon stimulation with (A) ATP (agonist of L-type voltage dependent Ca 2+ channels), (B) palmitoyl-oleoyl-phosphatidic acid (POPA; agonist of inositol 1,4,5-trisphosphate receptors) and (C) <t>4-chloro-3-methylphenol</t> (agonist of ryanodine receptor 2). Data is shown as means±SE. Results are representatives of three independent experiments performed in triplicates.
    4 Chloro 3 Methyl Phenol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore 4 isopropyl 3 methylphenol
    : Concentration-response curves in SIN-1 treated (70 min) and untreated (control) cardiomyocytes for [Ca 2+ ] i increase upon stimulation with (A) ATP (agonist of L-type voltage dependent Ca 2+ channels), (B) palmitoyl-oleoyl-phosphatidic acid (POPA; agonist of inositol 1,4,5-trisphosphate receptors) and (C) <t>4-chloro-3-methylphenol</t> (agonist of ryanodine receptor 2). Data is shown as means±SE. Results are representatives of three independent experiments performed in triplicates.
    4 Isopropyl 3 Methylphenol, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore 4 chloro m cresol
    [Ca 2+ ] i  is depressed by nonxanthine store-releasing compounds and after spontaneous calcium spikes.  A , 4-Chloro- m -cresol (500 μ m ) evoked a transient peak and a subsequent depression in [Ca 2+ ] i. B , Ratios (340/380 nm) from two simultaneously recorded
    4 Chloro M Cresol, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Treatment with a TRPV agonist dysregulates chemotaxis of B . pahangi infective larvae. (A) L3 parasites were extracted from mosquitoes and subjected to chemotaxis assays with or without 250 μM NAM treatment. Chemotaxis assays were performed by adding L3s to the middle of a 0.8% agarose plate (M), with either test cue (T) or water (C) added to the opposite sides of the plate. The plate was placed at 37°C for 30 minutes, scored after incubation, and the CI was calculated. (B) NAM dysregulates attraction of freshly extracted L3s to serum and NaCl but has no effect on aversion to 3-methyl-1-butanol. (C) NAM has no effect on translational movement of freshly extracted L3s. (D) Bpa-osm-9 expression is unchanged by in vitro culture at physiological or room temperature 4 HPE. (E) L3s cultured for 1 DPE do not show chemotaxis toward serum and have reduced motility on the chemotaxis plate when compared with untreated freshly extracted parasites ( p = 0.028, t test). Data for (A–C) represent the combined results of three independent biological replicates, except for the experiments with 3-methyl-1-butanol, which included two replicates (cohorts of mosquito infections). Data for (E) represent the results of two biological replicates. Each point represents a single chemotaxis plate with 8–10 L3s. Red diamonds and bars indicate the mean and standard error of the mean. Comparisons of means were performed using t tests (** p ≤ 0.01). Raw data for (B) through (E) can be found at https://github.com/zamanianlab/BrugiaChemo-ms . CI, chemotaxis index; DPE, days postextraction; FBS, fetal bovine serum; HPE, hours postextraction; L3, third stage larvae; NAM, nicotinamide; ns, not significant; TRP, transient receptor potential.

    Journal: PLoS Biology

    Article Title: Genetic and functional diversification of chemosensory pathway receptors in mosquito-borne filarial nematodes

    doi: 10.1371/journal.pbio.3000723

    Figure Lengend Snippet: Treatment with a TRPV agonist dysregulates chemotaxis of B . pahangi infective larvae. (A) L3 parasites were extracted from mosquitoes and subjected to chemotaxis assays with or without 250 μM NAM treatment. Chemotaxis assays were performed by adding L3s to the middle of a 0.8% agarose plate (M), with either test cue (T) or water (C) added to the opposite sides of the plate. The plate was placed at 37°C for 30 minutes, scored after incubation, and the CI was calculated. (B) NAM dysregulates attraction of freshly extracted L3s to serum and NaCl but has no effect on aversion to 3-methyl-1-butanol. (C) NAM has no effect on translational movement of freshly extracted L3s. (D) Bpa-osm-9 expression is unchanged by in vitro culture at physiological or room temperature 4 HPE. (E) L3s cultured for 1 DPE do not show chemotaxis toward serum and have reduced motility on the chemotaxis plate when compared with untreated freshly extracted parasites ( p = 0.028, t test). Data for (A–C) represent the combined results of three independent biological replicates, except for the experiments with 3-methyl-1-butanol, which included two replicates (cohorts of mosquito infections). Data for (E) represent the results of two biological replicates. Each point represents a single chemotaxis plate with 8–10 L3s. Red diamonds and bars indicate the mean and standard error of the mean. Comparisons of means were performed using t tests (** p ≤ 0.01). Raw data for (B) through (E) can be found at https://github.com/zamanianlab/BrugiaChemo-ms . CI, chemotaxis index; DPE, days postextraction; FBS, fetal bovine serum; HPE, hours postextraction; L3, third stage larvae; NAM, nicotinamide; ns, not significant; TRP, transient receptor potential.

    Article Snippet: Experiments using FBS and NaCl as cues included three biological replicates, and experiments with 3-methyl-1-butanol included two biological replicates.

    Techniques: Chemotaxis Assay, Incubation, Expressing, In Vitro, Cell Culture

    : Concentration-response curves in SIN-1 treated (70 min) and untreated (control) cardiomyocytes for [Ca 2+ ] i increase upon stimulation with (A) ATP (agonist of L-type voltage dependent Ca 2+ channels), (B) palmitoyl-oleoyl-phosphatidic acid (POPA; agonist of inositol 1,4,5-trisphosphate receptors) and (C) 4-chloro-3-methylphenol (agonist of ryanodine receptor 2). Data is shown as means±SE. Results are representatives of three independent experiments performed in triplicates.

    Journal: Redox Biology

    Article Title: Cross-talk between lipid and protein carbonylation in a dynamic cardiomyocyte model of mild nitroxidative stress

    doi: 10.1016/j.redox.2016.12.028

    Figure Lengend Snippet: : Concentration-response curves in SIN-1 treated (70 min) and untreated (control) cardiomyocytes for [Ca 2+ ] i increase upon stimulation with (A) ATP (agonist of L-type voltage dependent Ca 2+ channels), (B) palmitoyl-oleoyl-phosphatidic acid (POPA; agonist of inositol 1,4,5-trisphosphate receptors) and (C) 4-chloro-3-methylphenol (agonist of ryanodine receptor 2). Data is shown as means±SE. Results are representatives of three independent experiments performed in triplicates.

    Article Snippet: Horse serum, trypsin-EDTA solution, 2′,7′-dichlorofluorescin diacetate (DCFDA), thiazolyl blue tetrazolium bromide (MTT), β-nicotinamide adenine dinucleotide reduced (NADH), triton-X-100, DMSO, crystal violet solution (aqueous), 7-(diethylamino)-coumarin-3-carbohydrazide (CHH), paraformaldehyde (PFA), Nile Red, Hoechst 33258, thiourea, ß-mercaptoethanol, 2,2,2-trichlorethanol (TCE), hydrochloric acid, 2,4-dinitrophenyl hydrazine (DNPH), primary goat anti-DNP antibody, adenosine 5′-triphosphate (ATP), D-biotin, tert -butyl methyl ether (MTBE), formic acid, 4-chloro-3-methylphenol, copper (II) sulfate and all ammonium, magnesium, potassium and sodium salts were purchased from Sigma-Aldrich GmbH (Taufkirchen, Germany).

    Techniques: Concentration Assay

    [Ca 2+ ] i  is depressed by nonxanthine store-releasing compounds and after spontaneous calcium spikes.  A , 4-Chloro- m -cresol (500 μ m ) evoked a transient peak and a subsequent depression in [Ca 2+ ] i. B , Ratios (340/380 nm) from two simultaneously recorded

    Journal: The Journal of Neuroscience

    Article Title: Caffeine-Sensitive Calcium Stores Regulate Synaptic Transmission from Retinal Rod Photoreceptors

    doi: 10.1523/JNEUROSCI.19-17-07249.1999

    Figure Lengend Snippet: [Ca 2+ ] i is depressed by nonxanthine store-releasing compounds and after spontaneous calcium spikes. A , 4-Chloro- m -cresol (500 μ m ) evoked a transient peak and a subsequent depression in [Ca 2+ ] i. B , Ratios (340/380 nm) from two simultaneously recorded

    Article Snippet: 4-Chloro- m -cresol [4-chloro-3-methylphenol (4-CmC)] was obtained from Aldrich (Milwaukee, WI); thymol, glutamate dehydrogenase, gramicidin, and luciferase were from Sigma; and FMN reductase was obtained from Boehringer Mannheim (Indianapolis, IN).

    Techniques: