Journal: Redox Biology
Article Title: Cross-talk between lipid and protein carbonylation in a dynamic cardiomyocyte model of mild nitroxidative stress
Figure Lengend Snippet: : Concentration-response curves in SIN-1 treated (70 min) and untreated (control) cardiomyocytes for [Ca 2+ ] i increase upon stimulation with (A) ATP (agonist of L-type voltage dependent Ca 2+ channels), (B) palmitoyl-oleoyl-phosphatidic acid (POPA; agonist of inositol 1,4,5-trisphosphate receptors) and (C) 4-chloro-3-methylphenol (agonist of ryanodine receptor 2). Data is shown as means±SE. Results are representatives of three independent experiments performed in triplicates.
Article Snippet: Horse serum, trypsin-EDTA solution, 2′,7′-dichlorofluorescin diacetate (DCFDA), thiazolyl blue tetrazolium bromide (MTT), β-nicotinamide adenine dinucleotide reduced (NADH), triton-X-100, DMSO, crystal violet solution (aqueous), 7-(diethylamino)-coumarin-3-carbohydrazide (CHH), paraformaldehyde (PFA), Nile Red, Hoechst 33258, thiourea, ß-mercaptoethanol, 2,2,2-trichlorethanol (TCE), hydrochloric acid, 2,4-dinitrophenyl hydrazine (DNPH), primary goat anti-DNP antibody, adenosine 5′-triphosphate (ATP), D-biotin, tert -butyl methyl ether (MTBE), formic acid, 4-chloro-3-methylphenol, copper (II) sulfate and all ammonium, magnesium, potassium and sodium salts were purchased from Sigma-Aldrich GmbH (Taufkirchen, Germany).
Techniques: Concentration Assay