Journal: Experimental and Therapeutic Medicine

Article Title: Intensity-dependent effect of treadmill running on rat Achilles tendon

doi: 10.3892/etm.2018.6084

Figure Lengend Snippet: Representative images of immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P

Article Snippet: Sections were subsequently incubated with horseradish peroxidase conjugated goat anti-mouse Imunoglobulin G (1:200; cat. no. sc2005; Santa Cruz Biotechnology, CA, USA) for 1 h at room temperature, developed with 3,3′-Diaminobenzidine tetrahydrochloride (DAKO; Agilent Technologies, Inc., Santa Clara, CA, USA) and counter-stained in hematoxylin.

Techniques: Immunohistochemistry, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Intensity-dependent effect of treadmill running on rat Achilles tendon

doi: 10.3892/etm.2018.6084

Figure Lengend Snippet: Representative images of the immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col I in the rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups and (D) the statistical analysis. Scale bar represents 100 µm. *P

Article Snippet: Sections were subsequently incubated with horseradish peroxidase conjugated goat anti-mouse Imunoglobulin G (1:200; cat. no. sc2005; Santa Cruz Biotechnology, CA, USA) for 1 h at room temperature, developed with 3,3′-Diaminobenzidine tetrahydrochloride (DAKO; Agilent Technologies, Inc., Santa Clara, CA, USA) and counter-stained in hematoxylin.

Techniques: Immunohistochemistry, Staining

Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry

doi: 10.1089/mab.2017.0008

Figure Lengend Snippet: Immunohistochemical analysis of human kidney by PcMab-47. A section of kidney was incubated with 1 μg/mL of PcMab-47, followed by the Envision+ kit (A, B) . Color was developed using 3, 3-diaminobenzidine tetrahydrochloride, and counterstained with hematoxylin. Another section of kidney was also stained using hematoxylin and eosin (C, D) . Scale bar: 100 μm.

Article Snippet: Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (Agilent Technologies, Inc.) for 5 minutes, and then the sections were counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

Techniques: Immunohistochemistry, Incubation, Staining

Journal: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

Article Title: PcMab-47: Novel Antihuman Podocalyxin Monoclonal Antibody for Immunohistochemistry

doi: 10.1089/mab.2017.0008

Figure Lengend Snippet: Immunohistochemical analysis of human cancers by PcMab-47. Sections of colon cancer (A, B) and breast cancer (C, D) were incubated with 10 μg/mL of PcMab-47, followed by the Envision+ kit. Color was developed using 3, 3-diaminobenzidine tetrahydrochloride and counterstained with hematoxylin. Colon cancer (E, F) and breast cancer (G, H) were also stained using hematoxylin and eosin. Arrows: endothelial cells. Scale bar: 100 μm.

Article Snippet: Color was developed using 3, 3-diaminobenzidine tetrahydrochloride (Agilent Technologies, Inc.) for 5 minutes, and then the sections were counterstained with hematoxylin (Wako Pure Chemical Industries Ltd.).

Techniques: Immunohistochemistry, Incubation, Staining

Journal: The Journal of Veterinary Medical Science

Article Title: Establishment of serological test to detect antibody against ferret coronavirus

doi: 10.1292/jvms.16-0059

Figure Lengend Snippet: Immunoblot analysis using recombinant proteins. Three purified proteins, GST (lane 1), GST-N (1-179) (lane 2) and GST-N (180-374) (lane 3), were used as antigens. The result of CBB staining after SDS-PAGE analysis is shown in A. Immunoblot analysis was performed using plasma of ferret No.48 (B) and serum of No.22 (C) diluted to 1:1,000. Peroxidase-conjugated anti-ferret immunoglobulin was diluted to 1:1,000 and used as a secondary antibody. The reaction was visualized with 3,3′-diaminobenzidine tetrahydrochloride.

Article Snippet: The reaction was visualized using 3,3′-diaminobenzidine tetrahydrochloride (Wako).

Techniques: Recombinant, Purification, Staining, SDS Page