Article Title: KY1022, a small molecule destabilizing Ras via targeting the Wnt/β-catenin pathway, inhibits development of metastatic colorectal cancer
Figure Lengend Snippet: Both β-catenin and Ras levels are increased in adenocarcinoma, metastatic adenocarcinoma and tumor budding in colon cancer TMA specimens of normal mucosa, adenocarcinoma, metastatic adenocarcinoma, or tumor buddings were subjected to the IHC analyses by using β-catenin or Ras antibody followed by 3, 3'-diaminobenzidine (DAB) staining. A. Representative image of IHC analyses for β-catenin and Ras in normal tissues and at different stages of colorectal tumorigenesis. Boxes indicate the enlarged areas. Scale bar= 50μm. B., D. Quantitative analyses of positive signal were performed by comparing the H-Scores of staining for β-catenin and Ras in TMA samples. Normal Mucosa (n=2), Adenocarcinoma (n=26), Metastatic adenocarcinoma (n=24), Tumor budding (n=7). C. Representative images of IHC analyses for β-catenin and Ras in tumor budding compared with a neighboring adenocarcinoma in colon cancer TMA samples. The yellow boxes represent enlarged in the right panel. Scale bar= 50μm. D. Quantitative analysis for β-catenin and Ras was performed by comparing tumor budding with neighboring adenocarcinoma based on H-Score (n=10). Two-sided Student t test was used to determine statistical significance using GraphPad Prism5 Software. Error bars represent 95% confidence intervals.
Article Snippet: The samples were then incubated in ABC kit (Vector Laboratories) for 1 hour, stained with 3, 3′-diaminobenzidine (DAB; Dako) for 3−7 minutes and counterstained with Mayer's hematoxylin (Muto).
Techniques: Immunohistochemistry, Staining, Software