3 3 diaminobenzidine Search Results


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  • 99
    Vector Laboratories dab peroxidase hrp substrate kit with nickel 3 3 diaminobenzidine
    Dab Peroxidase Hrp Substrate Kit With Nickel 3 3 Diaminobenzidine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 3 3 diaminobenzidine
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    Millipore 3 3 diaminobenzidine
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    Agilent technologies 3 3 diaminobenzidine
    Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from <t>3,3'-diaminobenzidine.</t> Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.
    3 3 Diaminobenzidine, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 8814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 3 3 diaminobenzidine tetrahydrochloride
    Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from <t>3,3'-diaminobenzidine.</t> Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.
    3 3 Diaminobenzidine Tetrahydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 3 3 diaminobenzidine tetrahydrochloride
    Representative images of immunohistochemical staining, developed with <t>3,3′-Diaminobenzidine</t> <t>tetrahydrochloride</t> and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P
    3 3 Diaminobenzidine Tetrahydrochloride, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 2721 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore diaminobenzidine
    Representative images of immunohistochemical staining, developed with <t>3,3′-Diaminobenzidine</t> <t>tetrahydrochloride</t> and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P
    Diaminobenzidine, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 14403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 3 diaminobenzidine
    Representative images of immunohistochemical staining, developed with <t>3,3′-Diaminobenzidine</t> <t>tetrahydrochloride</t> and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P
    3 Diaminobenzidine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 3 3 diaminobenzidine dab
    Identifying <t>3,3′-diaminobenzidine</t> <t>(DAB)-stained</t> regions using the NanoSuit method. a – g Colon tissue immunostained using an antibody against smooth muscle actin with color development via DAB staining (brown). b Staining of the section shown in a with 1% gold (III) chloride. The DAB-staining intensity of the low-vacuum scanning electron microscopy (Lv-SEM) image was selectively enhanced as white color, taken in backscattered electron (BSE) mode. c Image of a control section without gold (III) chloride treatment. d DAB staining of the muscularis propria of a colon tissue section. e Lv-SEM image, taken in BSE mode. f DAB-stained section of a magnified area of the muscularis propria and blood vessels. g Three-dimensional (3D) structure of the correlative region shown in f . Lv-SEM image, taken in mixed BSE and SE mode. h DAB-stained dendritic cells (DCs) of the human epidermis. i Field emission-scanning electron microscopy (FE-SEM) image of DAB-stained DCs after treatment with 1% gold chloride, taken in yttrium–aluminum–garnet BSE mode. j 3D structure of DAB-stained DCs in an FE-SEM image, taken in secondary electron mode. The scale bars represent 2 mm ( a – c ), 500 ( d , e ), 50 ( f , g ), and 10 μm ( h – j )
    3 3 Diaminobenzidine Dab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 3 3 diaminobenzidine tetrahydrochloride
    Identifying <t>3,3′-diaminobenzidine</t> <t>(DAB)-stained</t> regions using the NanoSuit method. a – g Colon tissue immunostained using an antibody against smooth muscle actin with color development via DAB staining (brown). b Staining of the section shown in a with 1% gold (III) chloride. The DAB-staining intensity of the low-vacuum scanning electron microscopy (Lv-SEM) image was selectively enhanced as white color, taken in backscattered electron (BSE) mode. c Image of a control section without gold (III) chloride treatment. d DAB staining of the muscularis propria of a colon tissue section. e Lv-SEM image, taken in BSE mode. f DAB-stained section of a magnified area of the muscularis propria and blood vessels. g Three-dimensional (3D) structure of the correlative region shown in f . Lv-SEM image, taken in mixed BSE and SE mode. h DAB-stained dendritic cells (DCs) of the human epidermis. i Field emission-scanning electron microscopy (FE-SEM) image of DAB-stained DCs after treatment with 1% gold chloride, taken in yttrium–aluminum–garnet BSE mode. j 3D structure of DAB-stained DCs in an FE-SEM image, taken in secondary electron mode. The scale bars represent 2 mm ( a – c ), 500 ( d , e ), 50 ( f , g ), and 10 μm ( h – j )
    3 3 Diaminobenzidine Tetrahydrochloride, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 3 3 diaminobenzidine chromogen
    IL-36γ protein expression in gingival samples of healthy controls and periodontitis patients. Serial sections of gingival samples of healthy controls ( A ) and periodontitis patients ( B ) were immunostained with an isotype control antibody or with an anti-IL-36γ antibody. Secondary antibody goat anti-mouse was used. Specific binding was detected using <t>3,3-diaminobenzidine</t> chromogen. Sections were counterstained with Harris hematoxylin. EP: epithelium; CT: connective tissue, * are positive cells. Scale bar = 100 µm and = 25 µm.
    3 3 Diaminobenzidine Chromogen, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IL-36γ protein expression in gingival samples of healthy controls and periodontitis patients. Serial sections of gingival samples of healthy controls ( A ) and periodontitis patients ( B ) were immunostained with an isotype control antibody or with an anti-IL-36γ antibody. Secondary antibody goat anti-mouse was used. Specific binding was detected using <t>3,3-diaminobenzidine</t> chromogen. Sections were counterstained with Harris hematoxylin. EP: epithelium; CT: connective tissue, * are positive cells. Scale bar = 100 µm and = 25 µm.
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    Dojindo Labs 3 3 diaminobenzidine
    Double-staining of TIMP2 mRNA detected by in situ hybridization and hormones and S100 protein detected by immunohistochemistry in rat anterior pituitary gland. a, b : In situ hybridization for TIMP2. TIMP2-expressing cells were observed in the marginal layer surrounding Rathke’s cleft (RC) ( a ) and in the anterior lobe ( b ). c : Negative control with sense probe. d–i : In situ hybridization of TIMP2 and immunohistochemistry of ACTH ( d ), GH ( e ), prolactin ( f ), TSHβ ( g ), LHβ ( h ), and S100 protein (folliculostellate cells; i ). In situ hybridization with NBT/BCIP (blue) and immunostaining with <t>3,3'-diaminobenzidine</t> (brown). TIMP2 mRNA was colocalized with the prolactin, TSHβ, and S100 protein immunoreactions (arrows). Bar=10 μm ( a–i ).
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    Millipore 3 3 diaminobenzidine tablets
    Double-staining of TIMP2 mRNA detected by in situ hybridization and hormones and S100 protein detected by immunohistochemistry in rat anterior pituitary gland. a, b : In situ hybridization for TIMP2. TIMP2-expressing cells were observed in the marginal layer surrounding Rathke’s cleft (RC) ( a ) and in the anterior lobe ( b ). c : Negative control with sense probe. d–i : In situ hybridization of TIMP2 and immunohistochemistry of ACTH ( d ), GH ( e ), prolactin ( f ), TSHβ ( g ), LHβ ( h ), and S100 protein (folliculostellate cells; i ). In situ hybridization with NBT/BCIP (blue) and immunostaining with <t>3,3'-diaminobenzidine</t> (brown). TIMP2 mRNA was colocalized with the prolactin, TSHβ, and S100 protein immunoreactions (arrows). Bar=10 μm ( a–i ).
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    Image Search Results


    Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.

    Journal: PLoS ONE

    Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

    doi: 10.1371/journal.pone.0137043

    Figure Lengend Snippet: Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.

    Article Snippet: After blocking of unspecific sites (0,1% Avidin, 0,01% Biotin and Protein Block Serum-free; all obtained from DAKO, Hamburg, Germany), sections were stained with the first antibody for 2 h diluted in Antibody Diluent with Background Reducing Components (DAKO, Hamburg, Germany) followed by incubation with the biotinylated secondary antibody (30 min; DAKO, Hamburg, Germany) and the streptavidin-peroxidase reagent (30 min, DAKO, Hamburg, Germany) and finally 3,3'-diaminobenzidine (DAB; DAKO, Hamburg, Germany) used as the chromogen.

    Techniques: Staining, Immunohistochemistry, Mouse Assay, Labeling

    TUBB2B and DCDC2 are expressed in human kidneys. (A) Western-blot analysis to measure the existence of TUBB2B and DCDC2 in human kidneys. GAPDH = loading control. CC = Podocyte cell culture. WT = Wild type kidney. (B+C) Immunohistochemical staining of TUBB2B and DCDC2 in healthy human kidneys. 3,3'-diaminobenzidine (DAB) was used as chromogen (brown staining) and nuclei were stained with hematoxylin (blue). The black arrows mark a nuclear as well as cytoplasmic staining of TUBB2B and a cytoplasmic staining of DCDC2 in podocytes. A negative control was performed without the primary antibody ( S1 Fig ). T = tubule. EPC = parietal epithelial cell. Scale bar = 50 μm.

    Journal: PLoS ONE

    Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

    doi: 10.1371/journal.pone.0137043

    Figure Lengend Snippet: TUBB2B and DCDC2 are expressed in human kidneys. (A) Western-blot analysis to measure the existence of TUBB2B and DCDC2 in human kidneys. GAPDH = loading control. CC = Podocyte cell culture. WT = Wild type kidney. (B+C) Immunohistochemical staining of TUBB2B and DCDC2 in healthy human kidneys. 3,3'-diaminobenzidine (DAB) was used as chromogen (brown staining) and nuclei were stained with hematoxylin (blue). The black arrows mark a nuclear as well as cytoplasmic staining of TUBB2B and a cytoplasmic staining of DCDC2 in podocytes. A negative control was performed without the primary antibody ( S1 Fig ). T = tubule. EPC = parietal epithelial cell. Scale bar = 50 μm.

    Article Snippet: After blocking of unspecific sites (0,1% Avidin, 0,01% Biotin and Protein Block Serum-free; all obtained from DAKO, Hamburg, Germany), sections were stained with the first antibody for 2 h diluted in Antibody Diluent with Background Reducing Components (DAKO, Hamburg, Germany) followed by incubation with the biotinylated secondary antibody (30 min; DAKO, Hamburg, Germany) and the streptavidin-peroxidase reagent (30 min, DAKO, Hamburg, Germany) and finally 3,3'-diaminobenzidine (DAB; DAKO, Hamburg, Germany) used as the chromogen.

    Techniques: Western Blot, Cell Culture, Immunohistochemistry, Staining, Negative Control

    Tubb2b staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Tubb2b staining results from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A) Tubb2b brdp/brdp kidneys show a specific cytoplasmic Tubb2b expression in tubuli (T) and in podocytes (P), confirming the developmental defects seen with the Wt1, Nphs2, Nphs1 and Synpo stainings. (B) Tubb2b expression in wild type kidneys is restricted to the mature podocytes. Interestingly in the developing wild type kidney Tubb2b is not expressed in the early developmental stages of maturing podocytes. Note, that in murine podocytes nuclear Tubb2b seems much less expressed compared to human kidneys. Black asterisks: Enlarged section areas on the right. Scale bars = 50 μm.

    Journal: PLoS ONE

    Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

    doi: 10.1371/journal.pone.0137043

    Figure Lengend Snippet: Tubb2b staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Tubb2b staining results from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A) Tubb2b brdp/brdp kidneys show a specific cytoplasmic Tubb2b expression in tubuli (T) and in podocytes (P), confirming the developmental defects seen with the Wt1, Nphs2, Nphs1 and Synpo stainings. (B) Tubb2b expression in wild type kidneys is restricted to the mature podocytes. Interestingly in the developing wild type kidney Tubb2b is not expressed in the early developmental stages of maturing podocytes. Note, that in murine podocytes nuclear Tubb2b seems much less expressed compared to human kidneys. Black asterisks: Enlarged section areas on the right. Scale bars = 50 μm.

    Article Snippet: After blocking of unspecific sites (0,1% Avidin, 0,01% Biotin and Protein Block Serum-free; all obtained from DAKO, Hamburg, Germany), sections were stained with the first antibody for 2 h diluted in Antibody Diluent with Background Reducing Components (DAKO, Hamburg, Germany) followed by incubation with the biotinylated secondary antibody (30 min; DAKO, Hamburg, Germany) and the streptavidin-peroxidase reagent (30 min, DAKO, Hamburg, Germany) and finally 3,3'-diaminobenzidine (DAB; DAKO, Hamburg, Germany) used as the chromogen.

    Techniques: Staining, Immunohistochemistry, Mouse Assay, Expressing

    Immunoreactivity for GFAP and Iba-1. Representative images of GFAP + astrocytes and Iba-1 + microglia in the cortex and hippocampus of 5 month-old male F344 and HIV-1 Tg rats. 3,3-diaminobenzidine (brown) stained cells displayed normal process-bearing morphologies with no evidence of microglia activation or astrocyte hypertrophy. Quantitation of the % area occupied by Iba-1 + immunoreactive cells (mean +/− SD) in the cortex (n=6) and hippocampus (n=10) is provided within each representative image.

    Journal: Neurotoxicity research

    Article Title: Age-related decrease in tyrosine hydroxylase immunoreactivity in the substantia nigra and region-specific changes in microglia morphology in HIV-1 Tg rats

    doi: 10.1007/s12640-019-00077-z

    Figure Lengend Snippet: Immunoreactivity for GFAP and Iba-1. Representative images of GFAP + astrocytes and Iba-1 + microglia in the cortex and hippocampus of 5 month-old male F344 and HIV-1 Tg rats. 3,3-diaminobenzidine (brown) stained cells displayed normal process-bearing morphologies with no evidence of microglia activation or astrocyte hypertrophy. Quantitation of the % area occupied by Iba-1 + immunoreactive cells (mean +/− SD) in the cortex (n=6) and hippocampus (n=10) is provided within each representative image.

    Article Snippet: Rinsed sections were incubated using Vectastain Elite ABC kit for 90 min at RT, washed in 1xTBS and treated with 3-diaminobenzidine (DAB; Agilent Technologies, Santa Clara, CA) [10 mg DAB in 40 ml 1xTBS] and chromogen with nickel chloride amplification.

    Techniques: Staining, Activation Assay, Quantitation Assay

    Tyrosine Hydroxylase Immunoreactivity and Unbiased Stereology. A. Estimates of TH+ neuronal number between 2 and 8 months of age. Scatter graph of individual values (% of F344 control) at each age. Bar graphs of mean +/− SEM for 2–5 months (n=9) and 8 months (n=4–5). No difference was observed between 2–5 months of age. At 8 months-of-age, HIV-1 Tg rats showed significantly fewer TH+ neurons as compared to age-matched controls (p=0.002; n=4–5). B. Representative images of TH immunoreactivity (3,3-diaminobenzidine staining: brown, black) in the SN at 8 months-of-age. C. At 5 months of age, TH immunoreactivity in the SN showed no difference between F344 and HIV-1 rats with DAB staining or fluorescence (green) staining. Iba-1+ microglia showed similar morphology across groups as shown by immunostaining for Iba-1 (red, DAB). GFAP staining (DAB) showed no difference between groups.

    Journal: Neurotoxicity research

    Article Title: Age-related decrease in tyrosine hydroxylase immunoreactivity in the substantia nigra and region-specific changes in microglia morphology in HIV-1 Tg rats

    doi: 10.1007/s12640-019-00077-z

    Figure Lengend Snippet: Tyrosine Hydroxylase Immunoreactivity and Unbiased Stereology. A. Estimates of TH+ neuronal number between 2 and 8 months of age. Scatter graph of individual values (% of F344 control) at each age. Bar graphs of mean +/− SEM for 2–5 months (n=9) and 8 months (n=4–5). No difference was observed between 2–5 months of age. At 8 months-of-age, HIV-1 Tg rats showed significantly fewer TH+ neurons as compared to age-matched controls (p=0.002; n=4–5). B. Representative images of TH immunoreactivity (3,3-diaminobenzidine staining: brown, black) in the SN at 8 months-of-age. C. At 5 months of age, TH immunoreactivity in the SN showed no difference between F344 and HIV-1 rats with DAB staining or fluorescence (green) staining. Iba-1+ microglia showed similar morphology across groups as shown by immunostaining for Iba-1 (red, DAB). GFAP staining (DAB) showed no difference between groups.

    Article Snippet: Rinsed sections were incubated using Vectastain Elite ABC kit for 90 min at RT, washed in 1xTBS and treated with 3-diaminobenzidine (DAB; Agilent Technologies, Santa Clara, CA) [10 mg DAB in 40 ml 1xTBS] and chromogen with nickel chloride amplification.

    Techniques: Staining, Fluorescence, Immunostaining

    Representative images of immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Intensity-dependent effect of treadmill running on rat Achilles tendon

    doi: 10.3892/etm.2018.6084

    Figure Lengend Snippet: Representative images of immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P

    Article Snippet: Sections were subsequently incubated with horseradish peroxidase conjugated goat anti-mouse Imunoglobulin G (1:200; cat. no. sc2005; Santa Cruz Biotechnology, CA, USA) for 1 h at room temperature, developed with 3,3′-Diaminobenzidine tetrahydrochloride (DAKO; Agilent Technologies, Inc., Santa Clara, CA, USA) and counter-stained in hematoxylin.

    Techniques: Immunohistochemistry, Staining

    Representative images of the immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col I in the rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups and (D) the statistical analysis. Scale bar represents 100 µm. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Intensity-dependent effect of treadmill running on rat Achilles tendon

    doi: 10.3892/etm.2018.6084

    Figure Lengend Snippet: Representative images of the immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col I in the rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups and (D) the statistical analysis. Scale bar represents 100 µm. *P

    Article Snippet: Sections were subsequently incubated with horseradish peroxidase conjugated goat anti-mouse Imunoglobulin G (1:200; cat. no. sc2005; Santa Cruz Biotechnology, CA, USA) for 1 h at room temperature, developed with 3,3′-Diaminobenzidine tetrahydrochloride (DAKO; Agilent Technologies, Inc., Santa Clara, CA, USA) and counter-stained in hematoxylin.

    Techniques: Immunohistochemistry, Staining

    Identifying 3,3′-diaminobenzidine (DAB)-stained regions using the NanoSuit method. a – g Colon tissue immunostained using an antibody against smooth muscle actin with color development via DAB staining (brown). b Staining of the section shown in a with 1% gold (III) chloride. The DAB-staining intensity of the low-vacuum scanning electron microscopy (Lv-SEM) image was selectively enhanced as white color, taken in backscattered electron (BSE) mode. c Image of a control section without gold (III) chloride treatment. d DAB staining of the muscularis propria of a colon tissue section. e Lv-SEM image, taken in BSE mode. f DAB-stained section of a magnified area of the muscularis propria and blood vessels. g Three-dimensional (3D) structure of the correlative region shown in f . Lv-SEM image, taken in mixed BSE and SE mode. h DAB-stained dendritic cells (DCs) of the human epidermis. i Field emission-scanning electron microscopy (FE-SEM) image of DAB-stained DCs after treatment with 1% gold chloride, taken in yttrium–aluminum–garnet BSE mode. j 3D structure of DAB-stained DCs in an FE-SEM image, taken in secondary electron mode. The scale bars represent 2 mm ( a – c ), 500 ( d , e ), 50 ( f , g ), and 10 μm ( h – j )

    Journal: Laboratory Investigation; a Journal of Technical Methods and Pathology

    Article Title: The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

    doi: 10.1038/s41374-019-0309-7

    Figure Lengend Snippet: Identifying 3,3′-diaminobenzidine (DAB)-stained regions using the NanoSuit method. a – g Colon tissue immunostained using an antibody against smooth muscle actin with color development via DAB staining (brown). b Staining of the section shown in a with 1% gold (III) chloride. The DAB-staining intensity of the low-vacuum scanning electron microscopy (Lv-SEM) image was selectively enhanced as white color, taken in backscattered electron (BSE) mode. c Image of a control section without gold (III) chloride treatment. d DAB staining of the muscularis propria of a colon tissue section. e Lv-SEM image, taken in BSE mode. f DAB-stained section of a magnified area of the muscularis propria and blood vessels. g Three-dimensional (3D) structure of the correlative region shown in f . Lv-SEM image, taken in mixed BSE and SE mode. h DAB-stained dendritic cells (DCs) of the human epidermis. i Field emission-scanning electron microscopy (FE-SEM) image of DAB-stained DCs after treatment with 1% gold chloride, taken in yttrium–aluminum–garnet BSE mode. j 3D structure of DAB-stained DCs in an FE-SEM image, taken in secondary electron mode. The scale bars represent 2 mm ( a – c ), 500 ( d , e ), 50 ( f , g ), and 10 μm ( h – j )

    Article Snippet: After washing in phosphate-buffered solution (PBS; 137 mM NaCl, 10 mM phosphate, 2.7 mM KCl, pH 7.4), the sections were incubated with a peroxidase- or biotin-conjugated universal immunoenzyme polymer anti-mouse or anti-rabbit solution (Nichirei Biosciences) and visualized using streptavidin-conjugated gold (40 nm; Abcam, Cambridge, UK) or 3,3′-diaminobenzidine (DAB) (DAKO).

    Techniques: Staining, Electron Microscopy

    Direct observation of gold particles in immunostained sections using the NanoSuit method. a – c Staining of a breast cancer section with a human epidermal growth factor receptor 2 (HER2) score of 3+. a HER2 expression detected by 3,3′-diaminobenzidine (DAB) staining (brown). HER2 expression observed by detection of 40-nm gold particle signals as multiple white dots in a low-vacuum scanning electron microscopy (Lv-SEM) image taken in backscattered electron (BSE) mode ( b ), or in an FE-SEM taken in secondary electron mode ( c ). Staining of a breast cancer section with a HER2 score of 0. No HER2 expression was detected by DAB staining (brown) ( d ), in an Lv-SEM image taken in BSE mode ( e ), or in an FE-SEM image taken in secondary electron mode ( f ). g , h Detection of human cytomegalovirus (CMV) with an anti-gB antibody conjugated with 40-nm gold particles. The white square in g was magnified and multiple 40-nm gold particles on the surface of human CMV particles are shown in h . i F-actin detected with 40-nm gold particles on TNT-like structures. The scale bars represent 200 ( a , d ), 20 ( b , e ), 2 ( c , f , g , i ), and 1 μm ( h )

    Journal: Laboratory Investigation; a Journal of Technical Methods and Pathology

    Article Title: The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

    doi: 10.1038/s41374-019-0309-7

    Figure Lengend Snippet: Direct observation of gold particles in immunostained sections using the NanoSuit method. a – c Staining of a breast cancer section with a human epidermal growth factor receptor 2 (HER2) score of 3+. a HER2 expression detected by 3,3′-diaminobenzidine (DAB) staining (brown). HER2 expression observed by detection of 40-nm gold particle signals as multiple white dots in a low-vacuum scanning electron microscopy (Lv-SEM) image taken in backscattered electron (BSE) mode ( b ), or in an FE-SEM taken in secondary electron mode ( c ). Staining of a breast cancer section with a HER2 score of 0. No HER2 expression was detected by DAB staining (brown) ( d ), in an Lv-SEM image taken in BSE mode ( e ), or in an FE-SEM image taken in secondary electron mode ( f ). g , h Detection of human cytomegalovirus (CMV) with an anti-gB antibody conjugated with 40-nm gold particles. The white square in g was magnified and multiple 40-nm gold particles on the surface of human CMV particles are shown in h . i F-actin detected with 40-nm gold particles on TNT-like structures. The scale bars represent 200 ( a , d ), 20 ( b , e ), 2 ( c , f , g , i ), and 1 μm ( h )

    Article Snippet: After washing in phosphate-buffered solution (PBS; 137 mM NaCl, 10 mM phosphate, 2.7 mM KCl, pH 7.4), the sections were incubated with a peroxidase- or biotin-conjugated universal immunoenzyme polymer anti-mouse or anti-rabbit solution (Nichirei Biosciences) and visualized using streptavidin-conjugated gold (40 nm; Abcam, Cambridge, UK) or 3,3′-diaminobenzidine (DAB) (DAKO).

    Techniques: Staining, Expressing, Electron Microscopy

    IL-36γ protein expression in gingival samples of healthy controls and periodontitis patients. Serial sections of gingival samples of healthy controls ( A ) and periodontitis patients ( B ) were immunostained with an isotype control antibody or with an anti-IL-36γ antibody. Secondary antibody goat anti-mouse was used. Specific binding was detected using 3,3-diaminobenzidine chromogen. Sections were counterstained with Harris hematoxylin. EP: epithelium; CT: connective tissue, * are positive cells. Scale bar = 100 µm and = 25 µm.

    Journal: Scientific Reports

    Article Title: IL-36γ is a pivotal inflammatory player in periodontitis-associated bone loss

    doi: 10.1038/s41598-019-55595-9

    Figure Lengend Snippet: IL-36γ protein expression in gingival samples of healthy controls and periodontitis patients. Serial sections of gingival samples of healthy controls ( A ) and periodontitis patients ( B ) were immunostained with an isotype control antibody or with an anti-IL-36γ antibody. Secondary antibody goat anti-mouse was used. Specific binding was detected using 3,3-diaminobenzidine chromogen. Sections were counterstained with Harris hematoxylin. EP: epithelium; CT: connective tissue, * are positive cells. Scale bar = 100 µm and = 25 µm.

    Article Snippet: Specific binding was detected using 3,3-diaminobenzidine chromogen (Dako, UK).

    Techniques: Expressing, Binding Assay

    Double-staining of TIMP2 mRNA detected by in situ hybridization and hormones and S100 protein detected by immunohistochemistry in rat anterior pituitary gland. a, b : In situ hybridization for TIMP2. TIMP2-expressing cells were observed in the marginal layer surrounding Rathke’s cleft (RC) ( a ) and in the anterior lobe ( b ). c : Negative control with sense probe. d–i : In situ hybridization of TIMP2 and immunohistochemistry of ACTH ( d ), GH ( e ), prolactin ( f ), TSHβ ( g ), LHβ ( h ), and S100 protein (folliculostellate cells; i ). In situ hybridization with NBT/BCIP (blue) and immunostaining with 3,3'-diaminobenzidine (brown). TIMP2 mRNA was colocalized with the prolactin, TSHβ, and S100 protein immunoreactions (arrows). Bar=10 μm ( a–i ).

    Journal: Acta Histochemica et Cytochemica

    Article Title: Maintenance of the Extracellular Matrix in Rat Anterior Pituitary Gland: Identification of Cells Expressing Tissue Inhibitors of Metalloproteinases

    doi: 10.1267/ahc.15020

    Figure Lengend Snippet: Double-staining of TIMP2 mRNA detected by in situ hybridization and hormones and S100 protein detected by immunohistochemistry in rat anterior pituitary gland. a, b : In situ hybridization for TIMP2. TIMP2-expressing cells were observed in the marginal layer surrounding Rathke’s cleft (RC) ( a ) and in the anterior lobe ( b ). c : Negative control with sense probe. d–i : In situ hybridization of TIMP2 and immunohistochemistry of ACTH ( d ), GH ( e ), prolactin ( f ), TSHβ ( g ), LHβ ( h ), and S100 protein (folliculostellate cells; i ). In situ hybridization with NBT/BCIP (blue) and immunostaining with 3,3'-diaminobenzidine (brown). TIMP2 mRNA was colocalized with the prolactin, TSHβ, and S100 protein immunoreactions (arrows). Bar=10 μm ( a–i ).

    Article Snippet: The ABC method (Vector Laboratories) was performed with 3,3'-diaminobenzidine (Dojindo Laboratories, Kumamoto, Japan) as the substrate.

    Techniques: Double Staining, In Situ Hybridization, Immunohistochemistry, Expressing, Negative Control, Immunostaining

    Double-staining of TIMP3 mRNA detected by in situ hybridization and isolectin B4, desmin, hormones, and S100 protein detected by immunohistochemistry in rat anterior pituitary gland. a, b : In situ hybridization for TIMP3. TIMP3-expressing cells were observed in blood capillaries, perivascular spaces ( a ), and the anterior lobe ( b ). c : Negative control with sense probe. d–k : In situ hybridization of TIMP3 and immunohistochemistry of isolectin B4 (endothelial cells; d ), desmin ( e ), ACTH ( f ), GH ( g ), prolactin ( h ), TSHβ ( i ), LHβ ( j ), and S100 protein (folliculostellate cells; k ). In situ hybridization with NBT/BCIP (blue) and immunostaining with 3,3'-diaminobenzidine (brown). TIMP3 mRNA was colocalized with the isolectin B4, desmin, and S100 protein immunoreactions (arrows). Bar=10 μm ( a–k ).

    Journal: Acta Histochemica et Cytochemica

    Article Title: Maintenance of the Extracellular Matrix in Rat Anterior Pituitary Gland: Identification of Cells Expressing Tissue Inhibitors of Metalloproteinases

    doi: 10.1267/ahc.15020

    Figure Lengend Snippet: Double-staining of TIMP3 mRNA detected by in situ hybridization and isolectin B4, desmin, hormones, and S100 protein detected by immunohistochemistry in rat anterior pituitary gland. a, b : In situ hybridization for TIMP3. TIMP3-expressing cells were observed in blood capillaries, perivascular spaces ( a ), and the anterior lobe ( b ). c : Negative control with sense probe. d–k : In situ hybridization of TIMP3 and immunohistochemistry of isolectin B4 (endothelial cells; d ), desmin ( e ), ACTH ( f ), GH ( g ), prolactin ( h ), TSHβ ( i ), LHβ ( j ), and S100 protein (folliculostellate cells; k ). In situ hybridization with NBT/BCIP (blue) and immunostaining with 3,3'-diaminobenzidine (brown). TIMP3 mRNA was colocalized with the isolectin B4, desmin, and S100 protein immunoreactions (arrows). Bar=10 μm ( a–k ).

    Article Snippet: The ABC method (Vector Laboratories) was performed with 3,3'-diaminobenzidine (Dojindo Laboratories, Kumamoto, Japan) as the substrate.

    Techniques: Double Staining, In Situ Hybridization, Immunohistochemistry, Expressing, Negative Control, Immunostaining

    Double-staining of TIMP1 mRNA detected by in situ hybridization and hormones and S100 protein detected by immunohistochemistry in rat anterior pituitary gland. a, b : In situ hybridization for TIMP1. TIMP1-expressing cells were observed in the marginal layer ( a ) surrounding Rathke’s cleft (RC) and in the anterior lobe ( b ). c : Negative control with sense probe. d–i : In situ hybridization of TIMP1 and immunohistochemistry of adrenocorticotropic hormone (ACTH; d ), growth hormone (GH; e ), prolactin ( f ), thyroid-stimulating hormone β-subunit (TSHβ; g ), luteinizing hormone β-subunit (LHβ; h ), and S100 protein (folliculostellate cells; i ). In situ hybridization with NBT/BCIP (blue) and immunostaining with 3,3'-diaminobenzidine (brown). TIMP1 mRNA was colocalized with the S100 protein immunoreaction (arrows). Bar=10 μm ( a–i ).

    Journal: Acta Histochemica et Cytochemica

    Article Title: Maintenance of the Extracellular Matrix in Rat Anterior Pituitary Gland: Identification of Cells Expressing Tissue Inhibitors of Metalloproteinases

    doi: 10.1267/ahc.15020

    Figure Lengend Snippet: Double-staining of TIMP1 mRNA detected by in situ hybridization and hormones and S100 protein detected by immunohistochemistry in rat anterior pituitary gland. a, b : In situ hybridization for TIMP1. TIMP1-expressing cells were observed in the marginal layer ( a ) surrounding Rathke’s cleft (RC) and in the anterior lobe ( b ). c : Negative control with sense probe. d–i : In situ hybridization of TIMP1 and immunohistochemistry of adrenocorticotropic hormone (ACTH; d ), growth hormone (GH; e ), prolactin ( f ), thyroid-stimulating hormone β-subunit (TSHβ; g ), luteinizing hormone β-subunit (LHβ; h ), and S100 protein (folliculostellate cells; i ). In situ hybridization with NBT/BCIP (blue) and immunostaining with 3,3'-diaminobenzidine (brown). TIMP1 mRNA was colocalized with the S100 protein immunoreaction (arrows). Bar=10 μm ( a–i ).

    Article Snippet: The ABC method (Vector Laboratories) was performed with 3,3'-diaminobenzidine (Dojindo Laboratories, Kumamoto, Japan) as the substrate.

    Techniques: Double Staining, In Situ Hybridization, Immunohistochemistry, Expressing, Negative Control, Immunostaining