Journal: Poultry Science

Article Title: Resveratrol alleviates heat-stress-induced impairment of the jejunal mucosa through TLR4/MAPK signaling pathway in black-boned chicken

doi: 10.1016/j.psj.2023.103242

Figure Lengend Snippet: Expression of MKK3, p53, and MYD88 protein (A) and the relative gray values of MKK3/β-actin, p53 /β-actin, and MYD88/β-actin (B) in the jejunal mucosa. HT, high temperature treatment (37°C ± 2°C); HT+Res, HT+400 mg/kg resveratrol; NT, normal temperature treatment (24°C ± 2°C); NT+Res, NT+400 mg/kg resveratrol. One-way ANOVA and Duncan's multiple range test were used for comparing the differences among NT, NT+Res, HT, HT+Res groups. The values with different superscript letters are different ( P < 0.05). Error bars stand for the standard error of mean.

Article Snippet: The primary antibodies against β-actin (1:5000; 66009-1-Ig; ProteinTech, Wuhan, China), p53 (1:500; NBP2-29453; Novus Biologicals, Centennial, CO), MKK3 (1:1000; AF6327; Affinity Biosciences, Cincinnati, OH), or MYD88 (1:1000; 23230-1-AP; ProteinTech, Wuhan, China) were used.

Techniques: Expressing

Journal: Oncotarget

Article Title: Small molecule CP-31398 induces reactive oxygen species-dependent apoptosis in human multiple myeloma

doi: 10.18632/oncotarget.19508

Figure Lengend Snippet: (A, D) MM cell lines expressing wild-type p53 (MM1S, H929), mutant p53 (RPMI8226, U266, KMS5) or p53 null (KMS-11) were cultured with CP (0–20 μM). Cell viability was determined at 48 h using a WST-1 assay. (B) CP induced cytotoxicity in primary MM samples. Cell viability was expressed as percentage of the DMSO-treated control at 48 h using a WST-1 assay. (C) siRandom or sip53 transfected RPMI8226 cells were incubated with CP for 48 h and cell viability was assessed using a WST-1 assay. Mt.: Mutated, WT: wild type. Data represents means of 6 independent cultures. Error bars represent the SD. * P < 0.01.

Article Snippet: Non-silencing control siRNA (siRandom; #4390843, Thermo Scientific, Waltham, MA) and an siRNA targeting human p53 (sip53; sc-29453, Santa Cruz Biotechnology) were transfected into cell lines by nucleofection according to a previous report [ ]. siRNA-transfected MM cells were incubated for 24 h and treated with CP for 24-48 h. Then cell proliferation or apoptosis was measured using a WST-1 assay or an Annexin V/7AAD assay.

Techniques: Expressing, Mutagenesis, Cell Culture, WST-1 Assay, Transfection, Incubation

Journal: Oncotarget

Article Title: Small molecule CP-31398 induces reactive oxygen species-dependent apoptosis in human multiple myeloma

doi: 10.18632/oncotarget.19508

Figure Lengend Snippet: MM cell lines were treated with the IC 50 dosage of CP for 0, 6,12, and 24 h. Whole cell lysates were subjected to immunoblotting using anti-PARP, p53, p21, mdm2, PUMA, Bax, and beta-actin antibodies. FL; full length, CL; cleaved form.

Article Snippet: Non-silencing control siRNA (siRandom; #4390843, Thermo Scientific, Waltham, MA) and an siRNA targeting human p53 (sip53; sc-29453, Santa Cruz Biotechnology) were transfected into cell lines by nucleofection according to a previous report [ ]. siRNA-transfected MM cells were incubated for 24 h and treated with CP for 24-48 h. Then cell proliferation or apoptosis was measured using a WST-1 assay or an Annexin V/7AAD assay.

Techniques: Western Blot