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bacteroides thetaiotaomicron  (ATCC)
90
ATCC bacteroides thetaiotaomicron
Bacteroides Thetaiotaomicron, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacteroides thetaiotaomicron - by Bioz Stars, 2024-12
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uracil 5 monophosphate trisodium chem impex  (Chem Impex International)
95
Chem Impex International uracil 5 monophosphate trisodium chem impex
(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.
Uracil 5 Monophosphate Trisodium Chem Impex, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β arrestin1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology β arrestin1
(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.
β Arrestin1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β arrestin1/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
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β arrestin1 - by Bioz Stars, 2024-12
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pth342 sup45 276 437 galbd plasmid 29742  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.

Journal: bioRxiv

Article Title: TOR coordinates nucleotide availability with ribosome biogenesis in plants

doi: 10.1101/2020.01.30.927418

Figure Lengend Snippet: (A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.

Article Snippet: Methotrexate (Gainger), 6-mercaptopurine (Fisher Scientific), 5-fluorouracil (Fisher Scientific), 5-fluoroorotic acid (Fisher Scientific), adenosine-5′-monophosphate disodium (Fisher Scientific), cytidine-5′-monophosphate disodium (Chem-Impex), guanosine-5′-monophosphate disodium (Chem-Impex), inosine-5’-monophosphate (TGI), and uracil 5′-monophosphate trisodium (Chem-Impex) were prepared fresh in concentrated stock solutions that were then diluted in ½ MS media (Caisson Labs), pH = 5.7 with KOH, for application to seedlings.

Techniques: Activity Assay