29-742 Search Results


90
ATCC bacteroides thetaiotaomicron
Bacteroides Thetaiotaomicron, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bacteroides thetaiotaomicron/product/ATCC
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bacteroides thetaiotaomicron - by Bioz Stars, 2024-11
90/100 stars
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95
Chem Impex International uracil 5 monophosphate trisodium chem impex
(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.
Uracil 5 Monophosphate Trisodium Chem Impex, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uracil 5 monophosphate trisodium chem impex/product/Chem Impex International
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
uracil 5 monophosphate trisodium chem impex - by Bioz Stars, 2024-11
95/100 stars
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93
Santa Cruz Biotechnology β arrestin1
(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.
β Arrestin1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β arrestin1/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
β arrestin1 - by Bioz Stars, 2024-11
93/100 stars
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86
Thermo Fisher rabbit recombinant monoclonal mouse specific pdpn ma5 29742 thermofisher scientific
Source, application and concentration of antibodies.
Rabbit Recombinant Monoclonal Mouse Specific Pdpn Ma5 29742 Thermofisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit recombinant monoclonal mouse specific pdpn ma5 29742 thermofisher scientific/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit recombinant monoclonal mouse specific pdpn ma5 29742 thermofisher scientific - by Bioz Stars, 2024-11
86/100 stars
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N/A
Standard format Plasmid sent in bacteria as agar stab
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Image Search Results


(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.

Journal: bioRxiv

Article Title: TOR coordinates nucleotide availability with ribosome biogenesis in plants

doi: 10.1101/2020.01.30.927418

Figure Lengend Snippet: (A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.

Article Snippet: Methotrexate (Gainger), 6-mercaptopurine (Fisher Scientific), 5-fluorouracil (Fisher Scientific), 5-fluoroorotic acid (Fisher Scientific), adenosine-5′-monophosphate disodium (Fisher Scientific), cytidine-5′-monophosphate disodium (Chem-Impex), guanosine-5′-monophosphate disodium (Chem-Impex), inosine-5’-monophosphate (TGI), and uracil 5′-monophosphate trisodium (Chem-Impex) were prepared fresh in concentrated stock solutions that were then diluted in ½ MS media (Caisson Labs), pH = 5.7 with KOH, for application to seedlings.

Techniques: Activity Assay

Source, application and concentration of antibodies.

Journal: PLOS ONE

Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

doi: 10.1371/journal.pone.0291023

Figure Lengend Snippet: Source, application and concentration of antibodies.

Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

Techniques: Concentration Assay, Recombinant, Plasmid Preparation