27123 Search Results


93
ATCC genome announc
Genome Announc, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Addgene inc plasmid tc egfp β actin full length
( a ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and <t>β-actin-eGFP</t> (green) after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( b ) Left: FCI plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 9). Arrow indicates the time at which FCI attains positive values. Right: FCI plotted every 5 minutes for E-cadherin-RFP and β-actin-eGFP between 2 and 2.5 hours after calcium repletion. Error bars indicate mean ± s.e.m. ( c ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and β-actin-eGFP (green) treated with DECMA-1 antibody after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( d ) FCI values plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 6). Arrow indicates the time at which FCI is positive and remains positive through the rest of the experiment. Error bars indicate mean ± s.e.m.
Plasmid Tc Egfp β Actin Full Length, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid tc egfp β actin full length/product/Addgene inc
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86
DSMZ accession number dsm 27123
( a ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and <t>β-actin-eGFP</t> (green) after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( b ) Left: FCI plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 9). Arrow indicates the time at which FCI attains positive values. Right: FCI plotted every 5 minutes for E-cadherin-RFP and β-actin-eGFP between 2 and 2.5 hours after calcium repletion. Error bars indicate mean ± s.e.m. ( c ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and β-actin-eGFP (green) treated with DECMA-1 antibody after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( d ) FCI values plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 6). Arrow indicates the time at which FCI is positive and remains positive through the rest of the experiment. Error bars indicate mean ± s.e.m.
Accession Number Dsm 27123, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/accession number dsm 27123/product/DSMZ
Average 86 stars, based on 1 article reviews
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86
Addgene inc gfp β actin δzip δ3 utr addgene plasmid 27123 27124 and
( a ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and <t>β-actin-eGFP</t> (green) after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( b ) Left: FCI plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 9). Arrow indicates the time at which FCI attains positive values. Right: FCI plotted every 5 minutes for E-cadherin-RFP and β-actin-eGFP between 2 and 2.5 hours after calcium repletion. Error bars indicate mean ± s.e.m. ( c ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and β-actin-eGFP (green) treated with DECMA-1 antibody after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( d ) FCI values plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 6). Arrow indicates the time at which FCI is positive and remains positive through the rest of the experiment. Error bars indicate mean ± s.e.m.
Gfp β Actin δzip δ3 Utr Addgene Plasmid 27123 27124 And, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp β actin δzip δ3 utr addgene plasmid 27123 27124 and/product/Addgene inc
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Image Search Results


( a ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and β-actin-eGFP (green) after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( b ) Left: FCI plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 9). Arrow indicates the time at which FCI attains positive values. Right: FCI plotted every 5 minutes for E-cadherin-RFP and β-actin-eGFP between 2 and 2.5 hours after calcium repletion. Error bars indicate mean ± s.e.m. ( c ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and β-actin-eGFP (green) treated with DECMA-1 antibody after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( d ) FCI values plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 6). Arrow indicates the time at which FCI is positive and remains positive through the rest of the experiment. Error bars indicate mean ± s.e.m.

Journal: Scientific Reports

Article Title: Quantifying cadherin mechanotransduction machinery assembly/disassembly dynamics using fluorescence covariance analysis

doi: 10.1038/srep28822

Figure Lengend Snippet: ( a ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and β-actin-eGFP (green) after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( b ) Left: FCI plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 9). Arrow indicates the time at which FCI attains positive values. Right: FCI plotted every 5 minutes for E-cadherin-RFP and β-actin-eGFP between 2 and 2.5 hours after calcium repletion. Error bars indicate mean ± s.e.m. ( c ) Top panel: Montage of live cell movie (0 hour–12 hours) showing MDCK cells expressing E-cadherin-RFP (red) and β-actin-eGFP (green) treated with DECMA-1 antibody after calcium repletion. Bottom panel: +PDM maps of images from the top panel. ( d ) FCI values plotted every 30 minutes for E-cadherin-RFP and β-actin-eGFP after calcium repletion (n = 6). Arrow indicates the time at which FCI is positive and remains positive through the rest of the experiment. Error bars indicate mean ± s.e.m.

Article Snippet: MDCK cells stably expressing E-cadherin-RFP (a gift from Dr. James Nelson, Stanford) were transfected with the plasmid TC-eGFP-β-actin full length (Addgene ID: 27123) using Lipofectamine 2000 (Life Technologies) according to manufacturer guidelines.

Techniques: Expressing