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  • 92
    Novus Biologicals anti tbk1
    (A) RNA-seq analysis of the expression of genes in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs for 8 and 12 hours, respectively. Heatmap showing the expression of genes responsive to type I IFN in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs. (B) BMDMs from WT and Hectd3–/– mice were infected with F. novicida (100 MOI) for indicated times, and expression of Ifnb, Cxcl9, Mx1, and Tnfa was analyzed by qRT-PCR. (C) Production of IFN-β, IL-6, TNF-α, and IL-1β in uninfected (Med) and F. novicida–infected WT and Hectd3–/– BMDMs for 14 hours. (D) Immunoblot analysis of phosphorylation of <t>TBK1,</t> IRF3, STAT3, STAT1, IκBα, ERK, P38, IKKα, and JNK in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs at indicated times. Data represent 3 independent experiments (B–D) and are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
    Anti Tbk1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tbk1/product/Novus Biologicals
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti tbk1 - by Bioz Stars, 2024-07
    92/100 stars
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    93
    Proteintech mouse antibody against wnt1
    ( A ) Western blot showing the expression of wingless/integrated 1 <t>(Wnt1),</t> Wnt3a, Wnt5a, β-catenin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in five groups ( n = 3). G1: negative control group; G2: running group; G3: weight-bearing group; G4: positive control group; Neonatal: the neonatal bone, control. ( B ) The corresponding gray-scale value of Wnt1, Wnt3a, Wnt5a and β-catenin in the five groups ( n = 3); comparison among G1 and other three groups (G3, G4, and Neonatal), * P < 0.05.
    Mouse Antibody Against Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse antibody against wnt1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse antibody against wnt1 - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    93
    Proteintech wnt1
    Hyperin blocked wnt/β-catenin signal pathway. ( A ) The TOP/FOP luciferase activity was detected in AGS and MKN-45 cells. n=5, * P <0.05. ( B ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and <t>wnt1</t> in AGS and MKN-45 cells. n=5, * P <0.05.
    Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wnt1 - by Bioz Stars, 2024-07
    93/100 stars
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    93
    Proteintech wnt1 rabbit polyclonal antibody
    Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , <t>WNT1</t> (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.
    Wnt1 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt1 rabbit polyclonal antibody/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wnt1 rabbit polyclonal antibody - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    93
    Proteintech rabbit anti wnt1 polyclonal antibody
    Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , <t>WNT1</t> (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.
    Rabbit Anti Wnt1 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti wnt1 polyclonal antibody/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti wnt1 polyclonal antibody - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    (A) RNA-seq analysis of the expression of genes in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs for 8 and 12 hours, respectively. Heatmap showing the expression of genes responsive to type I IFN in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs. (B) BMDMs from WT and Hectd3–/– mice were infected with F. novicida (100 MOI) for indicated times, and expression of Ifnb, Cxcl9, Mx1, and Tnfa was analyzed by qRT-PCR. (C) Production of IFN-β, IL-6, TNF-α, and IL-1β in uninfected (Med) and F. novicida–infected WT and Hectd3–/– BMDMs for 14 hours. (D) Immunoblot analysis of phosphorylation of TBK1, IRF3, STAT3, STAT1, IκBα, ERK, P38, IKKα, and JNK in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs at indicated times. Data represent 3 independent experiments (B–D) and are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Journal: The Journal of Clinical Investigation

    Article Title: HECTD3 mediates TRAF3 polyubiquitination and type I interferon induction during bacterial infection

    doi: 10.1172/JCI120406

    Figure Lengend Snippet: (A) RNA-seq analysis of the expression of genes in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs for 8 and 12 hours, respectively. Heatmap showing the expression of genes responsive to type I IFN in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs. (B) BMDMs from WT and Hectd3–/– mice were infected with F. novicida (100 MOI) for indicated times, and expression of Ifnb, Cxcl9, Mx1, and Tnfa was analyzed by qRT-PCR. (C) Production of IFN-β, IL-6, TNF-α, and IL-1β in uninfected (Med) and F. novicida–infected WT and Hectd3–/– BMDMs for 14 hours. (D) Immunoblot analysis of phosphorylation of TBK1, IRF3, STAT3, STAT1, IκBα, ERK, P38, IKKα, and JNK in uninfected and F. novicida–infected WT and Hectd3–/– BMDMs at indicated times. Data represent 3 independent experiments (B–D) and are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Article Snippet: Cells were stained with anti-TRAF3 (Abcam, ab36988), anti-TBK1 (Novus Biologicals, 108A429), or anti–p-TBK1 (Cell Signaling Technology, 5483), all at 1:300 dilution, overnight at 4°C.

    Techniques: RNA Sequencing Assay, Expressing, Infection, Quantitative RT-PCR, Western Blot

    (A) Immunoblot analysis of HECTD3 and TRAF3 that coimmunoprecipitated with FLAG-TRAF3 or HA-HECTD3 from lysates of HEK293T cells transfected with plasmids, as indicated. (B) Co-IP analysis of polyubiquitination of TRAF3 mediated by HECTD3 in HEK293T cells transfected with plasmids as indicated. (C) Co-IP analysis of polyubiquitination of TRAF3 by WT and mutant HECTD3 (C823A) in HEK293T cells transfected with plasmids as indicated. (D) Co-IP analysis of endogenous TRAF3 with TBK1 and HECTD3 in WT and Hectd3–/– BMDMs with or without F. novicida infection for 6 hours. (E) Confocal microscopy analysis of colocalization of TBK1 and TRAF3, and phosphorylation of TBK1 cellular localization in WT and Hectd3–/– BMDMs with or without F. novicida infection for 6 hours. Scale bars: 10 μm. (F) Quantification of colocalization of TBK1 and TRAF3 in E **P < 0.01. (G) Co-IP analysis of polyubiquitination of endogenous TRAF3 in WT and Hectd3–/– BMDMs with or without F. novicida infection for 6 hours. Data represent 3 independent experiments for A–F and 2 independent experiments for G.

    Journal: The Journal of Clinical Investigation

    Article Title: HECTD3 mediates TRAF3 polyubiquitination and type I interferon induction during bacterial infection

    doi: 10.1172/JCI120406

    Figure Lengend Snippet: (A) Immunoblot analysis of HECTD3 and TRAF3 that coimmunoprecipitated with FLAG-TRAF3 or HA-HECTD3 from lysates of HEK293T cells transfected with plasmids, as indicated. (B) Co-IP analysis of polyubiquitination of TRAF3 mediated by HECTD3 in HEK293T cells transfected with plasmids as indicated. (C) Co-IP analysis of polyubiquitination of TRAF3 by WT and mutant HECTD3 (C823A) in HEK293T cells transfected with plasmids as indicated. (D) Co-IP analysis of endogenous TRAF3 with TBK1 and HECTD3 in WT and Hectd3–/– BMDMs with or without F. novicida infection for 6 hours. (E) Confocal microscopy analysis of colocalization of TBK1 and TRAF3, and phosphorylation of TBK1 cellular localization in WT and Hectd3–/– BMDMs with or without F. novicida infection for 6 hours. Scale bars: 10 μm. (F) Quantification of colocalization of TBK1 and TRAF3 in E **P < 0.01. (G) Co-IP analysis of polyubiquitination of endogenous TRAF3 in WT and Hectd3–/– BMDMs with or without F. novicida infection for 6 hours. Data represent 3 independent experiments for A–F and 2 independent experiments for G.

    Article Snippet: Cells were stained with anti-TRAF3 (Abcam, ab36988), anti-TBK1 (Novus Biologicals, 108A429), or anti–p-TBK1 (Cell Signaling Technology, 5483), all at 1:300 dilution, overnight at 4°C.

    Techniques: Western Blot, Transfection, Co-Immunoprecipitation Assay, Mutagenesis, Infection, Confocal Microscopy

    ( A ) Western blot showing the expression of wingless/integrated 1 (Wnt1), Wnt3a, Wnt5a, β-catenin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in five groups ( n = 3). G1: negative control group; G2: running group; G3: weight-bearing group; G4: positive control group; Neonatal: the neonatal bone, control. ( B ) The corresponding gray-scale value of Wnt1, Wnt3a, Wnt5a and β-catenin in the five groups ( n = 3); comparison among G1 and other three groups (G3, G4, and Neonatal), * P < 0.05.

    Journal: Metabolites

    Article Title: Exercise Promotes the Osteoinduction of HA/β-TCP Biomaterials via the Wnt Signaling Pathway

    doi: 10.3390/metabo10030090

    Figure Lengend Snippet: ( A ) Western blot showing the expression of wingless/integrated 1 (Wnt1), Wnt3a, Wnt5a, β-catenin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in five groups ( n = 3). G1: negative control group; G2: running group; G3: weight-bearing group; G4: positive control group; Neonatal: the neonatal bone, control. ( B ) The corresponding gray-scale value of Wnt1, Wnt3a, Wnt5a and β-catenin in the five groups ( n = 3); comparison among G1 and other three groups (G3, G4, and Neonatal), * P < 0.05.

    Article Snippet: After transferring the proteins onto a nitrocellulose membrane, the membrane was blocked with a 5% defatted milk solution and probed with mouse antibody against Wnt1 (1:1000, Proteintech, Rosemont, IL, USA), Wnt3a (1:1000, AFFINITY, Cincinnati, OH, USA), Wnt5a (1:600, Proteintech), β-catenin (1:1000, AFFINITY), runt-related transcription factor-2 (RUNX-2, 1:600, AFFINITY), osteopontin (OPN, 1:2000, Proteintech), osteocalcin (OCN, 1:1000, AFFINITY), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:5000, Santa Cruz, Santa Cruz, CA, USA), and then probed with a secondary antibody using ALP conjugated anti-mouse IgG (1:5000, Santa Cruz, USA).

    Techniques: Western Blot, Expressing, Negative Control, Positive Control

    Hyperin blocked wnt/β-catenin signal pathway. ( A ) The TOP/FOP luciferase activity was detected in AGS and MKN-45 cells. n=5, * P <0.05. ( B ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and wnt1 in AGS and MKN-45 cells. n=5, * P <0.05.

    Journal: Cancer Management and Research

    Article Title: Hyperin Controls the Development and Therapy of Gastric Cancer via Regulating Wnt/β-Catenin Signaling

    doi: 10.2147/CMAR.S270544

    Figure Lengend Snippet: Hyperin blocked wnt/β-catenin signal pathway. ( A ) The TOP/FOP luciferase activity was detected in AGS and MKN-45 cells. n=5, * P <0.05. ( B ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and wnt1 in AGS and MKN-45 cells. n=5, * P <0.05.

    Article Snippet: The primary antibody anti-Bcl2 (12,789-1-AP, Proteintech), anti-Bax (50,599-2-Ig, Proteintech), anti-cleaved-caspases3 (ab2302, abcam), anti-DKK1 (21,112-1-AP, Proteintech), anti-p-GSK3β (#3548, Cell Signaling Technology), anti-GSK3β (#12,456, Cell Signaling Technology), anti-β-catenin (51,067-2-AP, Proteintech), Wnt1 (27,935-1-AP, proteintech), and anti-GAPDH (60,004-1-Ig, Proteintech) incubation and second incubation were carried out according to the operation steps.

    Techniques: Luciferase, Activity Assay

    Hyperin significantly reduces tumor growth in vivo. ( A ) Tumors removed from the mice 30 days after Hyperin and Control treated, respectively n=5. ( B and C ) Tumor volume and weight were shown after the tumors were collected. n=5, * P <0.05. ( D ) H&E staining and TUNEL assay were performed. ( E ) Representative image of ki-67, caspase3, wnt1, and β-catenin IHC staining. ( F ) The protein level of Bcl2, Bax, cleaved-caspase3 in tumor tissues. n=5, * P <0.05. ( G ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and wnt1 in tumor tissues. n=5, * P <0.05.

    Journal: Cancer Management and Research

    Article Title: Hyperin Controls the Development and Therapy of Gastric Cancer via Regulating Wnt/β-Catenin Signaling

    doi: 10.2147/CMAR.S270544

    Figure Lengend Snippet: Hyperin significantly reduces tumor growth in vivo. ( A ) Tumors removed from the mice 30 days after Hyperin and Control treated, respectively n=5. ( B and C ) Tumor volume and weight were shown after the tumors were collected. n=5, * P <0.05. ( D ) H&E staining and TUNEL assay were performed. ( E ) Representative image of ki-67, caspase3, wnt1, and β-catenin IHC staining. ( F ) The protein level of Bcl2, Bax, cleaved-caspase3 in tumor tissues. n=5, * P <0.05. ( G ) The protein level of DKK1, NKD1, GSK3β, β-catenin, and wnt1 in tumor tissues. n=5, * P <0.05.

    Article Snippet: The primary antibody anti-Bcl2 (12,789-1-AP, Proteintech), anti-Bax (50,599-2-Ig, Proteintech), anti-cleaved-caspases3 (ab2302, abcam), anti-DKK1 (21,112-1-AP, Proteintech), anti-p-GSK3β (#3548, Cell Signaling Technology), anti-GSK3β (#12,456, Cell Signaling Technology), anti-β-catenin (51,067-2-AP, Proteintech), Wnt1 (27,935-1-AP, proteintech), and anti-GAPDH (60,004-1-Ig, Proteintech) incubation and second incubation were carried out according to the operation steps.

    Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry

    Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , WNT1 (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.

    Journal: Frontiers in Pharmacology

    Article Title: Thymopentin-Mediated Inhibition of Cancer Stem Cell Stemness Enhances the Cytotoxic Effect of Oxaliplatin on Colon Cancer Cells

    doi: 10.3389/fphar.2022.779715

    Figure Lengend Snippet: Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , WNT1 (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.

    Article Snippet: The membranes were incubated overnight at 4°C with primary antibody, and secondary antibodies anti-rabbit IgG HRP-conjugated (1: 3,000, 7074S, Cell Signaling Technology) and anti-mouse IgG HRP-conjugated (1: 3,000, 7076S, Cell Signaling Technology) were incubated at 25°C for incubate for 2 h. The primary antibodies used are Beta Catenin Rabbit Polyclonal Antibody (1: 1,000, 51067-2-AP, Proteintech), WNT1 Rabbit Polyclonal Antibody (1: 1,000, 27935-1-AP, Proteintech), Phospho-beta Catenin (Ser33/37/Thr41) antibody (1: 1,000, 9561, Cell Signaling Technology), AKT1 (Phospho-Ser473 + Tyr474) antibody (1: 1,000, 12669, Abcam), PI3K p110 (beta) polyclonal antibody (1: 1,000, 20584-1-AP, Proteintech), AKT1 polyclonal antibody (1: 1,000, 10176-2-AP, Proteintech), and GAPDH (1: 10,000, 60004-I-Ig, Proteintech).

    Techniques: Western Blot, Expressing