Journal: International Journal of Molecular Sciences
Article Title: Peptide-Mediated Gene Transfer into Marine Purple Photosynthetic Bacteria
Figure Lengend Snippet: The pDNA delivery into E. coli cells. ( a ) Ampicillin-resistant colonies of E. coli . The pDNA complex (BP100-(KH) 9 /pBS-ldhGFP) at N/P ratio 2.0 was introduced into 10 μM dTat-Sar-EED5 treated E. coli cells. ( b ) Effects of dTat-Sar-EED5 concentrations on the transformation efficiency of E. coli . The pDNA delivery was carried out under different concentrations of dTat-Sar-EED5 (0, 1, 10, 50 and 100 μM). The N/P ratio was set to 5.0, where a fixed amount of plasmid DNA (1 μg) was mixed with various amounts of peptide BP100-(KH) 9 . ‘pDNA only’ means plasmid DNA alone without BP100-(KH) 9 and dTat-Sar-EED5. ‘0 µM of dTat-Sar-EED5′ means pDNA complex (BP100-(KH) 9 /plasmid DNA) without dTat-Sar-EED5. Data were normalized by ‘0 μM dTat-Sar-EED5′. Data are the mean ± SD of three cell cultures. n.d., not detected.
Article Snippet: The pBBR1MCS-2 [ ] and pBS-ldhGFP (Addgene plasmid #27170 [ ]) were prepared using QIAprep Spin Miniprep kit (QIAGEN, Dusseldorf, Germany) according to the standard protocol.
Techniques: Transformation Assay, Plasmid Preparation