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    Interleukin 3 IL3 27 166 His tag mouse recombinant protein 20 µg
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    90
    ATCC e coli atcc 27166
    E Coli Atcc 27166, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli atcc 27166/product/ATCC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli atcc 27166 - by Bioz Stars, 2024-04
    90/100 stars
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    85
    Addgene inc hspa5 p495l
    (A) Neurons were transfected with plasmids expressing scrambled shRNA or Wfs1 shRNA, firefly luciferase constructs containing ATF6 or ATF4 binding sites or a XBP-1 splicing reporter, and Renilla luciferase. Firefly luciferase signal normalized to Renilla signal demonstrates a moderate increase in ATF6 and ATF4 reporter activity. (B) Positive control experiments in which the above-mentioned reporter systems and Renilla luciferase were co-transfected with ATF4, ATF6, or IRE1. (C) The mitochondrial fusion rate is reduced by Wfs1 shRNA and is restored by co-expressing wt <t>HSPA5</t> ( p = 0.001 for interaction, two-way ANOVA). (D) ATPase-deficient HSPA5 mutant (T37G) but not peptide binding-deficient mutant <t>(P495L)</t> restores the fusion rate reduced by Wfs1 shRNA. (E) HSPA5 overexpression attenuates mitophagy activated by Wfs1 silencing ( p = 0.012 for interaction). (F–H) Activation of the primary ER stress pathways by overexpression of ATF6, ATF4, or IRE1 modulates neither fusion rate (F), mitochondrial length (G), nor mitophagy (H). (I–L) Silencing of ATF6 or ATF4 modulates neither fusion rate (I, J) nor mitophagy (K, L). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with respective control groups, or ### p < 0.001 compared with the Wfs1 shRNA-transfected control group and ns non-significant compared with the Wfs1 shRNA-transfected control group. Underlying data is shown in .
    Hspa5 P495l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hspa5 p495l/product/Addgene inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hspa5 p495l - by Bioz Stars, 2024-04
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    Image Search Results


    (A) Neurons were transfected with plasmids expressing scrambled shRNA or Wfs1 shRNA, firefly luciferase constructs containing ATF6 or ATF4 binding sites or a XBP-1 splicing reporter, and Renilla luciferase. Firefly luciferase signal normalized to Renilla signal demonstrates a moderate increase in ATF6 and ATF4 reporter activity. (B) Positive control experiments in which the above-mentioned reporter systems and Renilla luciferase were co-transfected with ATF4, ATF6, or IRE1. (C) The mitochondrial fusion rate is reduced by Wfs1 shRNA and is restored by co-expressing wt HSPA5 ( p = 0.001 for interaction, two-way ANOVA). (D) ATPase-deficient HSPA5 mutant (T37G) but not peptide binding-deficient mutant (P495L) restores the fusion rate reduced by Wfs1 shRNA. (E) HSPA5 overexpression attenuates mitophagy activated by Wfs1 silencing ( p = 0.012 for interaction). (F–H) Activation of the primary ER stress pathways by overexpression of ATF6, ATF4, or IRE1 modulates neither fusion rate (F), mitochondrial length (G), nor mitophagy (H). (I–L) Silencing of ATF6 or ATF4 modulates neither fusion rate (I, J) nor mitophagy (K, L). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with respective control groups, or ### p < 0.001 compared with the Wfs1 shRNA-transfected control group and ns non-significant compared with the Wfs1 shRNA-transfected control group. Underlying data is shown in .

    Journal: PLoS Biology

    Article Title: Role of Mitochondrial Dynamics in Neuronal Development: Mechanism for Wolfram Syndrome

    doi: 10.1371/journal.pbio.1002511

    Figure Lengend Snippet: (A) Neurons were transfected with plasmids expressing scrambled shRNA or Wfs1 shRNA, firefly luciferase constructs containing ATF6 or ATF4 binding sites or a XBP-1 splicing reporter, and Renilla luciferase. Firefly luciferase signal normalized to Renilla signal demonstrates a moderate increase in ATF6 and ATF4 reporter activity. (B) Positive control experiments in which the above-mentioned reporter systems and Renilla luciferase were co-transfected with ATF4, ATF6, or IRE1. (C) The mitochondrial fusion rate is reduced by Wfs1 shRNA and is restored by co-expressing wt HSPA5 ( p = 0.001 for interaction, two-way ANOVA). (D) ATPase-deficient HSPA5 mutant (T37G) but not peptide binding-deficient mutant (P495L) restores the fusion rate reduced by Wfs1 shRNA. (E) HSPA5 overexpression attenuates mitophagy activated by Wfs1 silencing ( p = 0.012 for interaction). (F–H) Activation of the primary ER stress pathways by overexpression of ATF6, ATF4, or IRE1 modulates neither fusion rate (F), mitochondrial length (G), nor mitophagy (H). (I–L) Silencing of ATF6 or ATF4 modulates neither fusion rate (I, J) nor mitophagy (K, L). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with respective control groups, or ### p < 0.001 compared with the Wfs1 shRNA-transfected control group and ns non-significant compared with the Wfs1 shRNA-transfected control group. Underlying data is shown in .

    Article Snippet: ATeam (51958), ATF6 (11975), ATF6-GL3 (11976), ATF4 (26114), ATF4-luc (21850), WFS1 wt (13011), WFS1 P724L (13012), IRE1α (13009), D1ER (36325), D3cpv (36323), DRP1 K38A, EGFP-LC3 (24920), HSPA5 wt (27164), HSPA5 T37G (27165), HSPA5 P495L (27166), NRF2 (21555), ORAI1 (21638), PSD-95 (15463), and pAAV-hSyn-DsRedExpress (22907) were obtained from Addgene (Cambridge, MA).

    Techniques: Transfection, Expressing, shRNA, Luciferase, Construct, Binding Assay, Activity Assay, Positive Control, Mutagenesis, Over Expression, Activation Assay