2690 separations module hplc system Search Results


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  • 88
    Waters Corporation 2690 separation module
    2690 Separation Module, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2690 separation module/product/Waters Corporation
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    93
    Waters Corporation hplc system
    Hplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 7628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/Waters Corporation
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    90
    Waters Corporation 2690 hplc separation module
    2690 Hplc Separation Module, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2690 hplc separation module/product/Waters Corporation
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    85
    Waters Corporation alliance 2690 separations module
    Alliance 2690 Separations Module, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alliance 2690 separations module/product/Waters Corporation
    Average 85 stars, based on 9 article reviews
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    85
    Waters Corporation alliance 2690 hplc separation module
    Alliance 2690 Hplc Separation Module, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alliance 2690 hplc separation module/product/Waters Corporation
    Average 85 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
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    92
    Waters Corporation reversed phase hplc
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    Reversed Phase Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Waters Corporation separation modul 2690
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    Separation Modul 2690, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/separation modul 2690/product/Waters Corporation
    Average 91 stars, based on 13 article reviews
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    separation modul 2690 - by Bioz Stars, 2021-01
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    89
    Waters Corporation high pressure liquid chromatography hplc system
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    High Pressure Liquid Chromatography Hplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high pressure liquid chromatography hplc system/product/Waters Corporation
    Average 89 stars, based on 93 article reviews
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    85
    Waters Corporation model 2690 separations module hplc
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    Model 2690 Separations Module Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/model 2690 separations module hplc/product/Waters Corporation
    Average 85 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
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    88
    Waters Corporation 2487 dual absorbance detector
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    2487 Dual Absorbance Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2487 dual absorbance detector/product/Waters Corporation
    Average 88 stars, based on 397 article reviews
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    88
    Waters Corporation 2487 dual wavelength uv absorbance detector
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    2487 Dual Wavelength Uv Absorbance Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Waters Corporation 2690 separations module high performance liquid chromatography hplc system
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    2690 Separations Module High Performance Liquid Chromatography Hplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Waters Corporation 2996 photodiode array detector
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    2996 Photodiode Array Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 2208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Waters Corporation 474 scanning fluorescence detector
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    474 Scanning Fluorescence Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Waters Corporation 996 diode array detector
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    996 Diode Array Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/996 diode array detector/product/Waters Corporation
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    92
    Waters Corporation 996 photodiode array detector
    Turnover of <t>BODIPY-SM</t> after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to <t>HPLC</t> analysis. Results shown represent mean values from one representative experiment performed in duplicates.
    996 Photodiode Array Detector, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/996 photodiode array detector/product/Waters Corporation
    Average 92 stars, based on 1942 article reviews
    Price from $9.99 to $1999.99
    996 photodiode array detector - by Bioz Stars, 2021-01
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    Image Search Results


    Turnover of BODIPY-SM after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to HPLC analysis. Results shown represent mean values from one representative experiment performed in duplicates.

    Journal: Biochimica et Biophysica Acta

    Article Title: Endocytosis and intracellular processing of BODIPY-sphingomyelin by murine CATH.a neurons

    doi: 10.1016/j.bbalip.2013.08.007

    Figure Lengend Snippet: Turnover of BODIPY-SM after pulse labeling. CATH.a cells were cooled at 4 °C for 10 min, pulse-labeled with BODIPY-SM (1 μM) in serum-free culture medium for 30 min at 4 °C, washed, and chased for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, followed by lipid extraction from (A) cells and (B) the cellular supernatant. Lipid extracts were subjected to HPLC analysis. Results shown represent mean values from one representative experiment performed in duplicates.

    Article Snippet: BODIPY- and PYRENE-labeled lipids were separated by reversed-phase HPLC (Waters HPLC 2690 Separations Module) on a Kromasil C18 reversed-phase column (150 × 4.6 mm, 5 μm particle size; Altmann Analytik, München, Germany) equipped with the corresponding guard column (5 × 4.6 mm, 5 μm particle size) at a flow rate of 0.7–1 ml/min.

    Techniques: Labeling, High Performance Liquid Chromatography

    Analysis of PM-located BODIPY-SM. CATH.a cells were cooled at 4 °C for 10 min and pulse-labeled with BODIPY-SM (1 μM for B. cereus SMase treatment and 2 μM for the BE protocol) in serum-free culture medium for 30 min at 4 °C. (A) Cells were washed and chased in serum-free culture medium at 37 °C for 60 min to enable BODIPY-SM internalization. Cells were then subjected to B. cereus SMase treatment (150 mU/ml) in serum-free culture medium at 37 °C up to 4 h. Cellular lipid extracts were analyzed by HPLC. (B) After pulse labeling cells were washed and chased at 37 °C. At the indicated times cells were subjected to BE at 4 °C followed by BODIPY-SL analysis in the BE fractions and cell extracts by HPLC. Results shown represent mean values from one representative experiment performed in duplicates.

    Journal: Biochimica et Biophysica Acta

    Article Title: Endocytosis and intracellular processing of BODIPY-sphingomyelin by murine CATH.a neurons

    doi: 10.1016/j.bbalip.2013.08.007

    Figure Lengend Snippet: Analysis of PM-located BODIPY-SM. CATH.a cells were cooled at 4 °C for 10 min and pulse-labeled with BODIPY-SM (1 μM for B. cereus SMase treatment and 2 μM for the BE protocol) in serum-free culture medium for 30 min at 4 °C. (A) Cells were washed and chased in serum-free culture medium at 37 °C for 60 min to enable BODIPY-SM internalization. Cells were then subjected to B. cereus SMase treatment (150 mU/ml) in serum-free culture medium at 37 °C up to 4 h. Cellular lipid extracts were analyzed by HPLC. (B) After pulse labeling cells were washed and chased at 37 °C. At the indicated times cells were subjected to BE at 4 °C followed by BODIPY-SL analysis in the BE fractions and cell extracts by HPLC. Results shown represent mean values from one representative experiment performed in duplicates.

    Article Snippet: BODIPY- and PYRENE-labeled lipids were separated by reversed-phase HPLC (Waters HPLC 2690 Separations Module) on a Kromasil C18 reversed-phase column (150 × 4.6 mm, 5 μm particle size; Altmann Analytik, München, Germany) equipped with the corresponding guard column (5 × 4.6 mm, 5 μm particle size) at a flow rate of 0.7–1 ml/min.

    Techniques: Labeling, High Performance Liquid Chromatography

    Uptake and metabolism of fluorescent SM and Cer analogs during steady state labeling. CATH.a cells were incubated with (A) BODIPY-SM, (B) PYRENE-SM, or (C) BODIPY-Cer (1 μM each) for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, and cellular lipids were extracted with CHCl 3 /MeOH, dried, dissolved in ethanol, and analyzed by HPLC. Results shown represent mean values from one representative experiment performed in duplicates.

    Journal: Biochimica et Biophysica Acta

    Article Title: Endocytosis and intracellular processing of BODIPY-sphingomyelin by murine CATH.a neurons

    doi: 10.1016/j.bbalip.2013.08.007

    Figure Lengend Snippet: Uptake and metabolism of fluorescent SM and Cer analogs during steady state labeling. CATH.a cells were incubated with (A) BODIPY-SM, (B) PYRENE-SM, or (C) BODIPY-Cer (1 μM each) for the indicated time periods at 37 °C. Cells were washed with HBSS, scraped, and cellular lipids were extracted with CHCl 3 /MeOH, dried, dissolved in ethanol, and analyzed by HPLC. Results shown represent mean values from one representative experiment performed in duplicates.

    Article Snippet: BODIPY- and PYRENE-labeled lipids were separated by reversed-phase HPLC (Waters HPLC 2690 Separations Module) on a Kromasil C18 reversed-phase column (150 × 4.6 mm, 5 μm particle size; Altmann Analytik, München, Germany) equipped with the corresponding guard column (5 × 4.6 mm, 5 μm particle size) at a flow rate of 0.7–1 ml/min.

    Techniques: Labeling, Incubation, High Performance Liquid Chromatography

    Quantitation of BODIPY-SM endocytosis in the presence of pharmacological antagonists. CATH.a cells were incubated with BODIPY-SM (1 μM) and the corresponding inhibitors as described in Fig. 3 . Cells were scraped in ice-cold HBSS and lipids were extracted with CHCl 3 /MeOH. Dried lipids were re-dissolved in ethanol and analyzed by HPLC. Results are expressed as fluorescence intensity as % of control and represent mean ± SD (n = 3) of one representative experiment. ***p

    Journal: Biochimica et Biophysica Acta

    Article Title: Endocytosis and intracellular processing of BODIPY-sphingomyelin by murine CATH.a neurons

    doi: 10.1016/j.bbalip.2013.08.007

    Figure Lengend Snippet: Quantitation of BODIPY-SM endocytosis in the presence of pharmacological antagonists. CATH.a cells were incubated with BODIPY-SM (1 μM) and the corresponding inhibitors as described in Fig. 3 . Cells were scraped in ice-cold HBSS and lipids were extracted with CHCl 3 /MeOH. Dried lipids were re-dissolved in ethanol and analyzed by HPLC. Results are expressed as fluorescence intensity as % of control and represent mean ± SD (n = 3) of one representative experiment. ***p

    Article Snippet: BODIPY- and PYRENE-labeled lipids were separated by reversed-phase HPLC (Waters HPLC 2690 Separations Module) on a Kromasil C18 reversed-phase column (150 × 4.6 mm, 5 μm particle size; Altmann Analytik, München, Germany) equipped with the corresponding guard column (5 × 4.6 mm, 5 μm particle size) at a flow rate of 0.7–1 ml/min.

    Techniques: Quantitation Assay, Incubation, High Performance Liquid Chromatography, Fluorescence

    Uptake of HDL-associated BODIPY-SM. (A) Protein lysates obtained from porcine brain microvascular endothelial cells (positive control; lane 1) and CATH.a cells incubated for 24 h in the absence (lane 2) or presence (lane 3) of serum were separated on 10% SDS gels and transferred to PVDF membranes for subsequent detection using anti-SR-BI as a primary antibody. Immunoreactive bands were detected at an apparent molecular weight of approx. 80 and 160 kDa (arrows). (B) Cells were incubated in serum-free medium (a–d) or in the presence of hypertonic sucrose (e) for 30 min followed by addition of BODIPY-SM/DiI-labeled HDL (50 μg HDL protein/ml) for (a) 5 min, (b, d, and e) 60 min, or (c) 240 min at 37 °C. Cells in (d) received a 40-fold excess of unlabeled HDL. After washing with HBSS cells were subjected to LSM analysis. All micrographs were recorded with the same laser intensity. (C) Cells were incubated in serum-free medium (a–d) or in the presence of hypertonic sucrose (e) for 30 min followed by addition of BODIPY-SM/Cy3-labeled HDL (50 μg HDL protein/ml) for (a) 5 min, (b, d, and e) 60 min, or (c) 240 min at 37 °C. Cells in (d) received a 40-fold excess of unlabeled HDL. After washing with HBSS cells were subjected to LSM analysis. All micrographs were recorded with the same laser intensity. (D) Cells were incubated with BODIPY-SM-labeled HDL (50 μg HDL protein/ml) for the indicated times at 37 °C. Cellular lipids were extracted, dissolved in ethanol, and analyzed by HPLC. Results shown represent mean values from one representative experiment performed in duplicates.

    Journal: Biochimica et Biophysica Acta

    Article Title: Endocytosis and intracellular processing of BODIPY-sphingomyelin by murine CATH.a neurons

    doi: 10.1016/j.bbalip.2013.08.007

    Figure Lengend Snippet: Uptake of HDL-associated BODIPY-SM. (A) Protein lysates obtained from porcine brain microvascular endothelial cells (positive control; lane 1) and CATH.a cells incubated for 24 h in the absence (lane 2) or presence (lane 3) of serum were separated on 10% SDS gels and transferred to PVDF membranes for subsequent detection using anti-SR-BI as a primary antibody. Immunoreactive bands were detected at an apparent molecular weight of approx. 80 and 160 kDa (arrows). (B) Cells were incubated in serum-free medium (a–d) or in the presence of hypertonic sucrose (e) for 30 min followed by addition of BODIPY-SM/DiI-labeled HDL (50 μg HDL protein/ml) for (a) 5 min, (b, d, and e) 60 min, or (c) 240 min at 37 °C. Cells in (d) received a 40-fold excess of unlabeled HDL. After washing with HBSS cells were subjected to LSM analysis. All micrographs were recorded with the same laser intensity. (C) Cells were incubated in serum-free medium (a–d) or in the presence of hypertonic sucrose (e) for 30 min followed by addition of BODIPY-SM/Cy3-labeled HDL (50 μg HDL protein/ml) for (a) 5 min, (b, d, and e) 60 min, or (c) 240 min at 37 °C. Cells in (d) received a 40-fold excess of unlabeled HDL. After washing with HBSS cells were subjected to LSM analysis. All micrographs were recorded with the same laser intensity. (D) Cells were incubated with BODIPY-SM-labeled HDL (50 μg HDL protein/ml) for the indicated times at 37 °C. Cellular lipids were extracted, dissolved in ethanol, and analyzed by HPLC. Results shown represent mean values from one representative experiment performed in duplicates.

    Article Snippet: BODIPY- and PYRENE-labeled lipids were separated by reversed-phase HPLC (Waters HPLC 2690 Separations Module) on a Kromasil C18 reversed-phase column (150 × 4.6 mm, 5 μm particle size; Altmann Analytik, München, Germany) equipped with the corresponding guard column (5 × 4.6 mm, 5 μm particle size) at a flow rate of 0.7–1 ml/min.

    Techniques: Positive Control, Incubation, Molecular Weight, Labeling, High Performance Liquid Chromatography