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  • 85
    ATCC a parasiticus atcc 26865
    A Parasiticus Atcc 26865, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a parasiticus atcc 26865/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a parasiticus atcc 26865 - by Bioz Stars, 2024-07
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    93
    Cell Signaling Technology Inc ogdh
    a. Isolated rat neonatal cardiac myocytes (rNCM), b. mouse adult cardiac myocytes (mACM), c. human iPSC-derived cardiac myocytes (hiPSC-CM), and d. SW620 colon cancer cells, were cultured, fixed, and immune-stained with <t>the</t> <t>antibodies</t> for the proteins indicated on the left <t>(anti-OGDH,</t> -IDH2, -PDHA1, and -ACAA2, all in red), in addition to phalloidin (green), and DAPI (blue). For each, the left panels show the staining for OGDH, IDH2, PDHA1, or ACAA2 (red) and the right panel, their overlay with phalloidin (except for the mACM) and DAPI. The cells were then imaged using confocal microscopy. e. Ten and a half-day-old mouse embryos were immune-stained for OGDH (green), α-actinin (red), and DAPI (blue), each shown separately or in an overlay (rightmost). The scale bars represent: a-d. 20 µm and e. 50 µm.
    Ogdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ogdh/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
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    ogdh - by Bioz Stars, 2024-07
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    86
    Addgene inc pqcxip hbt ubiquitin
    a. Isolated rat neonatal cardiac myocytes (rNCM), b. mouse adult cardiac myocytes (mACM), c. human iPSC-derived cardiac myocytes (hiPSC-CM), and d. SW620 colon cancer cells, were cultured, fixed, and immune-stained with <t>the</t> <t>antibodies</t> for the proteins indicated on the left <t>(anti-OGDH,</t> -IDH2, -PDHA1, and -ACAA2, all in red), in addition to phalloidin (green), and DAPI (blue). For each, the left panels show the staining for OGDH, IDH2, PDHA1, or ACAA2 (red) and the right panel, their overlay with phalloidin (except for the mACM) and DAPI. The cells were then imaged using confocal microscopy. e. Ten and a half-day-old mouse embryos were immune-stained for OGDH (green), α-actinin (red), and DAPI (blue), each shown separately or in an overlay (rightmost). The scale bars represent: a-d. 20 µm and e. 50 µm.
    Pqcxip Hbt Ubiquitin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pqcxip hbt ubiquitin/product/Addgene inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pqcxip hbt ubiquitin - by Bioz Stars, 2024-07
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    Image Search Results


    a. Isolated rat neonatal cardiac myocytes (rNCM), b. mouse adult cardiac myocytes (mACM), c. human iPSC-derived cardiac myocytes (hiPSC-CM), and d. SW620 colon cancer cells, were cultured, fixed, and immune-stained with the antibodies for the proteins indicated on the left (anti-OGDH, -IDH2, -PDHA1, and -ACAA2, all in red), in addition to phalloidin (green), and DAPI (blue). For each, the left panels show the staining for OGDH, IDH2, PDHA1, or ACAA2 (red) and the right panel, their overlay with phalloidin (except for the mACM) and DAPI. The cells were then imaged using confocal microscopy. e. Ten and a half-day-old mouse embryos were immune-stained for OGDH (green), α-actinin (red), and DAPI (blue), each shown separately or in an overlay (rightmost). The scale bars represent: a-d. 20 µm and e. 50 µm.

    Journal: bioRxiv

    Article Title: H2A.Z-dependent and -independent recruitment of metabolic enzymes to chromatin required for histone modifications

    doi: 10.1101/553297

    Figure Lengend Snippet: a. Isolated rat neonatal cardiac myocytes (rNCM), b. mouse adult cardiac myocytes (mACM), c. human iPSC-derived cardiac myocytes (hiPSC-CM), and d. SW620 colon cancer cells, were cultured, fixed, and immune-stained with the antibodies for the proteins indicated on the left (anti-OGDH, -IDH2, -PDHA1, and -ACAA2, all in red), in addition to phalloidin (green), and DAPI (blue). For each, the left panels show the staining for OGDH, IDH2, PDHA1, or ACAA2 (red) and the right panel, their overlay with phalloidin (except for the mACM) and DAPI. The cells were then imaged using confocal microscopy. e. Ten and a half-day-old mouse embryos were immune-stained for OGDH (green), α-actinin (red), and DAPI (blue), each shown separately or in an overlay (rightmost). The scale bars represent: a-d. 20 µm and e. 50 µm.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight: OGDH (Cell Signaling Technology, cat # 26865, 1:100 dilution) and alpha-cardiac actin (Sigma, cat # A9357, 1:300 dilution) diluted in the blocking buffer.

    Techniques: Isolation, Derivative Assay, Cell Culture, Staining, Confocal Microscopy

    The Human Protein Atlas is a Swedish-based project that includes antibody-based imaging of human tissue and cell lines, and is open access for scientists allowing free use of the data, given that it is properly cited [Ref. ]. Shown are images from that project that include normal human heart sections immuno-stained with a. anti-OGDH and b. anti-PDHB, c. human breast cancer sections immuno-stained with anti-HADBH, d. A431 cells immuno-stained with anti-OGDH, and e. A-431 cells immuno-stained with anti-MRSP36. Direct links to the web pages are listed beneath each image. Note, different antibodies had differential affinities to the nuclear v. mitochondrial form of a given protein, as demonstrated in our data f. We also stained the human colon cancer cell line SW620 with the a second OGDH antibody that targets the N-terminus v. the C-terminus, used in the main , for validation of the data.

    Journal: bioRxiv

    Article Title: H2A.Z-dependent and -independent recruitment of metabolic enzymes to chromatin required for histone modifications

    doi: 10.1101/553297

    Figure Lengend Snippet: The Human Protein Atlas is a Swedish-based project that includes antibody-based imaging of human tissue and cell lines, and is open access for scientists allowing free use of the data, given that it is properly cited [Ref. ]. Shown are images from that project that include normal human heart sections immuno-stained with a. anti-OGDH and b. anti-PDHB, c. human breast cancer sections immuno-stained with anti-HADBH, d. A431 cells immuno-stained with anti-OGDH, and e. A-431 cells immuno-stained with anti-MRSP36. Direct links to the web pages are listed beneath each image. Note, different antibodies had differential affinities to the nuclear v. mitochondrial form of a given protein, as demonstrated in our data f. We also stained the human colon cancer cell line SW620 with the a second OGDH antibody that targets the N-terminus v. the C-terminus, used in the main , for validation of the data.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight: OGDH (Cell Signaling Technology, cat # 26865, 1:100 dilution) and alpha-cardiac actin (Sigma, cat # A9357, 1:300 dilution) diluted in the blocking buffer.

    Techniques: Imaging, Staining

    Cardiac myocytes were infected with a 10-20 moi of recombinant adenoviruses harboring turbo-GFP (tGFP) or a. wt ACAA-tGFP or an NLS mutant (mtACAA2-tGFP), b. wt OGDH or an NLS mutant (mtOGDH-tGFP), c. wt IDH2-tGFP, tGFP-fused cDNAs. After 18 h, the cellular protein/organelles were fractionated into cytosol (cyto), mitochondrial and membrane (mito), nuclear (nuc), and chromatin-bound (chrom) protein fractions that were then analyzed by Western blotting for the proteins listed on the left of each panel. The fusion proteins were detected by anti-GFP (upper panels, a-c) and anti-ACAA2, anti-OGDH, and anti-IDH2 (second panels, a-c), which also detect the endogenous proteins. AKT1, VDAC1, TFIIB or Pol II, and H3, were immunodetected for use as internal controls for the corresponding cell fractions. The signals for the d. tGFP, and tGFP-fusion proteins e. ACAA2-tGFP, f. mtACAA2-tGFP, g. OGDH-tGFP, h. mtOGDH-tGFP, i. IDH2-tGFP (top panels), were quantified using imageJ, normalized to internal controls, and plotted as the mean ± SEM of % total protein in all 4 fractions. Error bars represent SEM, N=3 from 3 repeats. * p = 0.0095 vs. wt tGFP-fusion, # p ≤ 0.05 vs. tGFP, in corresponding fractions.

    Journal: bioRxiv

    Article Title: H2A.Z-dependent and -independent recruitment of metabolic enzymes to chromatin required for histone modifications

    doi: 10.1101/553297

    Figure Lengend Snippet: Cardiac myocytes were infected with a 10-20 moi of recombinant adenoviruses harboring turbo-GFP (tGFP) or a. wt ACAA-tGFP or an NLS mutant (mtACAA2-tGFP), b. wt OGDH or an NLS mutant (mtOGDH-tGFP), c. wt IDH2-tGFP, tGFP-fused cDNAs. After 18 h, the cellular protein/organelles were fractionated into cytosol (cyto), mitochondrial and membrane (mito), nuclear (nuc), and chromatin-bound (chrom) protein fractions that were then analyzed by Western blotting for the proteins listed on the left of each panel. The fusion proteins were detected by anti-GFP (upper panels, a-c) and anti-ACAA2, anti-OGDH, and anti-IDH2 (second panels, a-c), which also detect the endogenous proteins. AKT1, VDAC1, TFIIB or Pol II, and H3, were immunodetected for use as internal controls for the corresponding cell fractions. The signals for the d. tGFP, and tGFP-fusion proteins e. ACAA2-tGFP, f. mtACAA2-tGFP, g. OGDH-tGFP, h. mtOGDH-tGFP, i. IDH2-tGFP (top panels), were quantified using imageJ, normalized to internal controls, and plotted as the mean ± SEM of % total protein in all 4 fractions. Error bars represent SEM, N=3 from 3 repeats. * p = 0.0095 vs. wt tGFP-fusion, # p ≤ 0.05 vs. tGFP, in corresponding fractions.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight: OGDH (Cell Signaling Technology, cat # 26865, 1:100 dilution) and alpha-cardiac actin (Sigma, cat # A9357, 1:300 dilution) diluted in the blocking buffer.

    Techniques: Infection, Recombinant, Mutagenesis, Western Blot

    SW480 human colon cancer cell were infected with a 10-30 moi of recombinant adenoviruses harboring turbo-GFP (tGFP) or a. wt ACAA-tGFP or an NLS mutant (mtACAA2-tGFP), b. wt OGDH. In a., the cells were incubated in either glucose-containing (fatty acid and serum-free) or in palmitate-BSA (glucose-free and serum-free) medium, as indicated at the top of the lanes. After 18 h, the cellular protein/organelles were fractionated into cytosol (cyto), mitochondrial and membrane (mito), nuclear (nuc), and chromatin-bound (chrom) protein fractions that were then analyzed by Western blotting for the proteins listed on the left of each panel. The fusion proteins were detected by anti-GFP (upper panels, a-b) and anti-ACAA2 or anti-OGDH (second panels, a-b), which also detect the endogenous proteins. AKT1, VDAC1, Pol II, were immunodetected for their use as internal controls for the corresponding cell fractions: cytosol, mitochondria and, nuclear and chromatin, respectively.

    Journal: bioRxiv

    Article Title: H2A.Z-dependent and -independent recruitment of metabolic enzymes to chromatin required for histone modifications

    doi: 10.1101/553297

    Figure Lengend Snippet: SW480 human colon cancer cell were infected with a 10-30 moi of recombinant adenoviruses harboring turbo-GFP (tGFP) or a. wt ACAA-tGFP or an NLS mutant (mtACAA2-tGFP), b. wt OGDH. In a., the cells were incubated in either glucose-containing (fatty acid and serum-free) or in palmitate-BSA (glucose-free and serum-free) medium, as indicated at the top of the lanes. After 18 h, the cellular protein/organelles were fractionated into cytosol (cyto), mitochondrial and membrane (mito), nuclear (nuc), and chromatin-bound (chrom) protein fractions that were then analyzed by Western blotting for the proteins listed on the left of each panel. The fusion proteins were detected by anti-GFP (upper panels, a-b) and anti-ACAA2 or anti-OGDH (second panels, a-b), which also detect the endogenous proteins. AKT1, VDAC1, Pol II, were immunodetected for their use as internal controls for the corresponding cell fractions: cytosol, mitochondria and, nuclear and chromatin, respectively.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight: OGDH (Cell Signaling Technology, cat # 26865, 1:100 dilution) and alpha-cardiac actin (Sigma, cat # A9357, 1:300 dilution) diluted in the blocking buffer.

    Techniques: Infection, Recombinant, Mutagenesis, Incubation, Western Blot

    Both mouse heart tissue and human SW480 colon cancer cell line, were subjected to H2A.Z and OGDH ChIP-Seq using the same antibodies and chromatin concentration. The resulting sequence Tags form both reactions were aligned across the coordinates for the same genes in the mouse and human genomes, as indicated. Two regions are shown, a. the first showing the PRKAB2, FMO4, and CHD1L genes in the human cells and mouse tissue, where OGDH co-localizes with H2A.Z at the TSS in all three genes in the latter, however, OGDH is absent in from the FMO5 in the human genome, b. the second region encompasses PKN2, GTF2B, CCBL2, GBP1-5,7 genes that show conserved co-localization of OGDH and H2A.Z at the TSS of the former 3 genes in the mouse and human, but differs between species for the GBP genes, which have no OGDH in of the human cells, with a relatively small peak of H2A.Z at the TSS of GBP1-4. These data reveal that the co-localization of H2A.Z and OGDH at TSSs of key specific genes are conserved between species.

    Journal: bioRxiv

    Article Title: H2A.Z-dependent and -independent recruitment of metabolic enzymes to chromatin required for histone modifications

    doi: 10.1101/553297

    Figure Lengend Snippet: Both mouse heart tissue and human SW480 colon cancer cell line, were subjected to H2A.Z and OGDH ChIP-Seq using the same antibodies and chromatin concentration. The resulting sequence Tags form both reactions were aligned across the coordinates for the same genes in the mouse and human genomes, as indicated. Two regions are shown, a. the first showing the PRKAB2, FMO4, and CHD1L genes in the human cells and mouse tissue, where OGDH co-localizes with H2A.Z at the TSS in all three genes in the latter, however, OGDH is absent in from the FMO5 in the human genome, b. the second region encompasses PKN2, GTF2B, CCBL2, GBP1-5,7 genes that show conserved co-localization of OGDH and H2A.Z at the TSS of the former 3 genes in the mouse and human, but differs between species for the GBP genes, which have no OGDH in of the human cells, with a relatively small peak of H2A.Z at the TSS of GBP1-4. These data reveal that the co-localization of H2A.Z and OGDH at TSSs of key specific genes are conserved between species.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight: OGDH (Cell Signaling Technology, cat # 26865, 1:100 dilution) and alpha-cardiac actin (Sigma, cat # A9357, 1:300 dilution) diluted in the blocking buffer.

    Techniques: ChIP-sequencing, Concentration Assay, Sequencing