Journal: Antibiotics

Article Title: Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production in Streptomyces ambofaciens

doi: 10.3390/antibiotics10080947

Figure Lengend Snippet: Genomic map showing the distribution of monocistronic and polycistronic transcription units, and ncRNAs’ loci in the linear chromosome of S. ambofaciens ATCC 23877.

Article Snippet: The transcriptome of S. ambofaciens ATCC 23877 linear chromosome was redefined from RNAseq data of bacteria growing in YS broth at different time points (48 h, 72 h, 96 h, and 120 h) based on Rockhopper software [ , , ] predictions.

Techniques:

Journal: Antibiotics

Article Title: Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production in Streptomyces ambofaciens

doi: 10.3390/antibiotics10080947

Figure Lengend Snippet: Differential expression of ncRNAs during the growth of S. ambofaciens ATCC 23877. ( a , b ) S. ambofaciens ATCC 23877 was cultivated in YS, and ncRNA levels were determined after 48 h, 72 h, 96 h, and 120 h . Log2Fold changes of sRNA ( a ) and asRNA I ( b ) resulting differentially expressed in at least one timepoint were reported. The sRNAs and asRNAs I are arranged on the x-axis according to the sRNA and asRNA I ID numbering reported in .

Article Snippet: The transcriptome of S. ambofaciens ATCC 23877 linear chromosome was redefined from RNAseq data of bacteria growing in YS broth at different time points (48 h, 72 h, 96 h, and 120 h) based on Rockhopper software [ , , ] predictions.

Techniques: Expressing

Journal: Antibiotics

Article Title: Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production in Streptomyces ambofaciens

doi: 10.3390/antibiotics10080947

Figure Lengend Snippet: Growth curves and antibiotic production in S. ambofaciens ATCC 23877 (w.t.), and derivative ΩpirA, and ΩpirA rpoB(R) mutants. ( a – d ) Strains were cultivated in either YS ( a , b ) or SFM ( c , d ) broths, and biomass ( a , c ), spiramycin ( b ), and antimycin ( d ) values were determined at different time points. The values were calculated as the mean of three or more experiments. The errors bar indicates the standard deviation.

Article Snippet: The transcriptome of S. ambofaciens ATCC 23877 linear chromosome was redefined from RNAseq data of bacteria growing in YS broth at different time points (48 h, 72 h, 96 h, and 120 h) based on Rockhopper software [ , , ] predictions.

Techniques: Standard Deviation

Journal: Antibiotics

Article Title: Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production in Streptomyces ambofaciens

doi: 10.3390/antibiotics10080947

Figure Lengend Snippet: Time-course cluster analysis of RNAseq data in S. ambofaciens ATCC 23877 (w.t., red), and derivative ΩpirA (green), and ΩpirA rpoB(R) (blue) mutants. maSigPro identified the expression pattern of 1166 variable genes during the time course subdivided into 9 clusters and median profile of each cluster was inferred from the expression patterns.

Article Snippet: The transcriptome of S. ambofaciens ATCC 23877 linear chromosome was redefined from RNAseq data of bacteria growing in YS broth at different time points (48 h, 72 h, 96 h, and 120 h) based on Rockhopper software [ , , ] predictions.

Techniques: Expressing

Journal: Antibiotics

Article Title: Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production in Streptomyces ambofaciens

doi: 10.3390/antibiotics10080947

Figure Lengend Snippet: Differential expression of sRNas and asRNAs of class I in S. ambofaciens ATCC 23877 (w.t.), and derivative ΩpirA and ΩpirA rpoB(R) mutants at 48 h (left panel) and 120 h (right panel). . Log2fold changes of the two mutants compared to the w.t. strain were reported in the plots as indicated by the labels.

Article Snippet: The transcriptome of S. ambofaciens ATCC 23877 linear chromosome was redefined from RNAseq data of bacteria growing in YS broth at different time points (48 h, 72 h, 96 h, and 120 h) based on Rockhopper software [ , , ] predictions.

Techniques: Expressing