Journal: Standards in Genomic Sciences
Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Figure Lengend Snippet: Appearance of DNA libraries from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of DNA libraries sizes (generated with the Agilent 2200 TapeStation DNA genomics Kit) prepared for sequencing with the PacBio SMRTbell 10kb Template Preparation Kit on the Agilent NGS Workstation. The average library size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb) is displayed on the software screen. Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram
Article Snippet: The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes.
Techniques: Generated, Sequencing, Next-Generation Sequencing, Software, Quantitation Assay, Marker