2200 tapestation Agilent Technologies Search Results


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  • 95
    Agilent technologies agilent technologies 2200 tapestation
    Electrophoretic analysis of WGA libraries by Agilent <t>2200</t> <t>TapeStation</t> Instrument. The libraries were obtained by DOP-PCR (lane 1), PicoPlex (lane 2), and iDOP-PCR (lane 3) from 15 pg of human gDNA. M –DNA marker “Genomic DNA ScreenTape”.
    Agilent Technologies 2200 Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies 2200 tapestation system
    Appearance of sheared DNA from Agilent <t>2200</t> <t>TapeStation</t> analysis. Representative electropherogram ( a ) and virtual gel ( b ) of sheared bacterial genomic DNA was generated with the Agilent 2200 TapeStation genomic DNA Kit with the average shearing size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram
    2200 Tapestation System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Agilent technologies 2200 bioanalyzer tapestation
    Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to <t>Tapestation</t> <t>bioanalyzer</t> showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).
    2200 Bioanalyzer Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Agilent technologies tapestation 2200 r6k
    Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to <t>Tapestation</t> <t>bioanalyzer</t> showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).
    Tapestation 2200 R6k, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies 2200 tapestation d1000 screentapes
    Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to <t>Tapestation</t> <t>bioanalyzer</t> showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).
    2200 Tapestation D1000 Screentapes, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies 2200 tapestation p200 screentape assay
    Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to <t>Tapestation</t> <t>bioanalyzer</t> showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).
    2200 Tapestation P200 Screentape Assay, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies 2200 tapestation analysis
    Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to <t>Tapestation</t> <t>bioanalyzer</t> showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).
    2200 Tapestation Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Agilent technologies tapestation 2200 d1k
    Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to <t>Tapestation</t> <t>bioanalyzer</t> showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).
    Tapestation 2200 D1k, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies tapestation system
    Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to <t>Tapestation</t> <t>bioanalyzer</t> showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).
    Tapestation System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2200 tapestation nucleic acid system
    Appearance of sheared DNA from Agilent <t>2200</t> <t>TapeStation</t> analysis. Representative electropherogram ( a ) and virtual gel ( b ) of sheared bacterial genomic DNA was generated with the Agilent 2200 TapeStation genomic DNA Kit with the average shearing size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram
    2200 Tapestation Nucleic Acid System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 87/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Agilent technologies tapestation 2200 platform
    Appearance of sheared DNA from Agilent <t>2200</t> <t>TapeStation</t> analysis. Representative electropherogram ( a ) and virtual gel ( b ) of sheared bacterial genomic DNA was generated with the Agilent 2200 TapeStation genomic DNA Kit with the average shearing size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram
    Tapestation 2200 Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2200 tapestation automated electrophoresis system
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Automated Electrophoresis System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Agilent technologies bioanlayzer 2200 tapestation
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    Bioanlayzer 2200 Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Agilent technologies 2200 tapestation d1000
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation D1000, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 83/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Agilent technologies 2200 tapestation nucleic acids system
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Nucleic Acids System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies rnascreen tapestation 2200 system
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    Rnascreen Tapestation 2200 System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 83/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies model 2200 tapestation
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    Model 2200 Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2200 tapestation genomic dna screentape assay
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Genomic Dna Screentape Assay, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 87/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Agilent technologies 2200 tapesation
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapesation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies 2200 rna tapestation system
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Rna Tapestation System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2200 tapestation assay kit
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Assay Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Agilent technologies 2200 tapestation electrophoresis
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Electrophoresis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2200 tapestation rna r6k assay
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Rna R6k Assay, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Agilent technologies tapestation automated electrophoresis analysis system
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    Tapestation Automated Electrophoresis Analysis System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies electrophoresis system
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    Electrophoresis System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies 2200 tapestation machine
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Machine, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Agilent technologies 2200 tapestation analyzer
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Analyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Agilent technologies hi sensitivity d1k tapestation
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    Hi Sensitivity D1k Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 83/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies 2200 tapestation electrophoretic platform
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Electrophoretic Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Agilent technologies 2200 tapestation bioanalyzer system
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    2200 Tapestation Bioanalyzer System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Agilent technologies genomics 2200 tapestation
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    Genomics 2200 Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
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    Agilent technologies tape based electrophoresis
    Comparison of electropherogram of gDNA using the Agilent <t>2200</t> <t>TapeStation.</t> gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,
    Tape Based Electrophoresis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Electrophoretic analysis of WGA libraries by Agilent 2200 TapeStation Instrument. The libraries were obtained by DOP-PCR (lane 1), PicoPlex (lane 2), and iDOP-PCR (lane 3) from 15 pg of human gDNA. M –DNA marker “Genomic DNA ScreenTape”.

    Journal: PLoS ONE

    Article Title: Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA

    doi: 10.1371/journal.pone.0184507

    Figure Lengend Snippet: Electrophoretic analysis of WGA libraries by Agilent 2200 TapeStation Instrument. The libraries were obtained by DOP-PCR (lane 1), PicoPlex (lane 2), and iDOP-PCR (lane 3) from 15 pg of human gDNA. M –DNA marker “Genomic DNA ScreenTape”.

    Article Snippet: After the amplifications, the yields of WGA reactions were quantified using the PicoGreen® dsDNA Assay Kit (Molecular Probes, Eugene, OR, USA) and by Agilent 2200 TapeStation Instrument with Genomic DNA ScreenTape System (Agilent Technologies, Waldbronn, Germany).

    Techniques: Whole Genome Amplification, Degenerate Oligonucleotide–primed Polymerase Chain Reaction, Polymerase Chain Reaction, Marker

    Electrophoresis of DNAs by Agilent 2200 TapeStation. DNAs (100 ng/lane) were electrophoresed on an Agilent 2200 TapeStation. The DNA integrity number (DIN) indicates the fragmentation of genomic DNA on a scale from 1 to 10. A high DIN indicates highly intact DNA, and a low DIN indicates strongly degraded DNA. Lane 1, Frozen-H1; lane 2, Frozen-H2; lane 3, Frozen-H3; lane 4, FFPE-H1; lane 5, FFPE-H2; lane 6, FFPE-H3; lane 7, Trizol-h1; lane 8, Trizol-h2; lane 9, Trizol-h4; lane 10, Trizol-h6; and lane 11, Trizol-h7.

    Journal: PLoS ONE

    Article Title: Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions

    doi: 10.1371/journal.pone.0150528

    Figure Lengend Snippet: Electrophoresis of DNAs by Agilent 2200 TapeStation. DNAs (100 ng/lane) were electrophoresed on an Agilent 2200 TapeStation. The DNA integrity number (DIN) indicates the fragmentation of genomic DNA on a scale from 1 to 10. A high DIN indicates highly intact DNA, and a low DIN indicates strongly degraded DNA. Lane 1, Frozen-H1; lane 2, Frozen-H2; lane 3, Frozen-H3; lane 4, FFPE-H1; lane 5, FFPE-H2; lane 6, FFPE-H3; lane 7, Trizol-h1; lane 8, Trizol-h2; lane 9, Trizol-h4; lane 10, Trizol-h6; and lane 11, Trizol-h7.

    Article Snippet: The size distribution of 100 ng of DNA was also analyzed by electrophoresis using an Agilent 2200 TapeStation ( and ).

    Techniques: Electrophoresis, Formalin-fixed Paraffin-Embedded

    Example Agilent TapeStation 2200 electropherograms. ( a ) Electropherogram of an optimal post-PCR sample at Step 47. ( b ) Electropherogram from Step 47 showing higher molecular species resulting from too many PCR cycles. See Experimental design for details

    Journal: Nature protocols

    Article Title: Detecting ultralow-frequency mutations by Duplex Sequencing

    doi: 10.1038/nprot.2014.170

    Figure Lengend Snippet: Example Agilent TapeStation 2200 electropherograms. ( a ) Electropherogram of an optimal post-PCR sample at Step 47. ( b ) Electropherogram from Step 47 showing higher molecular species resulting from too many PCR cycles. See Experimental design for details

    Article Snippet: 28 Remove 2 μl from the A-tailed library and quantify the amount of DNA using the high-sensitivity kits for either the Agilent TapeStation 2200 or Agilent Bioanalyzer 2100, according to the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction

    The Tapestation results of amplified DNA from single Nostoc sp., Gloeocapsa sp. and Sphaerocystis sp. cells using the optimized protocol. The DNA was not degraded. Three single cells from each species were shown.

    Journal: Micromachines

    Article Title: The Development of an Effective Bacterial Single-Cell Lysis Method Suitable for Whole Genome Amplification in Microfluidic Platforms

    doi: 10.3390/mi9080367

    Figure Lengend Snippet: The Tapestation results of amplified DNA from single Nostoc sp., Gloeocapsa sp. and Sphaerocystis sp. cells using the optimized protocol. The DNA was not degraded. Three single cells from each species were shown.

    Article Snippet: After the expected success of lysis and amplification of single C. glutamicum was achieved, we assessed the integrity of the amplified DNA from single C. glutamicum cells in Tapestation (Agilent 2200, D1000 Screentape, Santa Clara, CA, USA), and the results show that the DNA was not degraded ( ).

    Techniques: Amplification

    Appearance of sheared DNA from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of sheared bacterial genomic DNA was generated with the Agilent 2200 TapeStation genomic DNA Kit with the average shearing size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: Appearance of sheared DNA from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of sheared bacterial genomic DNA was generated with the Agilent 2200 TapeStation genomic DNA Kit with the average shearing size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Article Snippet: The Agilent 2200 TapeStation System with the Genomic DNA assay was used to assess size and concentration of each sample as shown in Fig. , where an electropherogram overlay and virtual gel images are shown for the four model organisms, together with the DIN calculated by the TapeStation software.

    Techniques: Generated, Quantitation Assay, Marker

    Appearance of DNA libraries from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of DNA libraries sizes (generated with the Agilent 2200 TapeStation DNA genomics Kit) prepared for sequencing with the PacBio SMRTbell 10kb Template Preparation Kit on the Agilent NGS Workstation. The average library size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb) is displayed on the software screen. Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: Appearance of DNA libraries from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of DNA libraries sizes (generated with the Agilent 2200 TapeStation DNA genomics Kit) prepared for sequencing with the PacBio SMRTbell 10kb Template Preparation Kit on the Agilent NGS Workstation. The average library size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb) is displayed on the software screen. Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Article Snippet: The Agilent 2200 TapeStation System with the Genomic DNA assay was used to assess size and concentration of each sample as shown in Fig. , where an electropherogram overlay and virtual gel images are shown for the four model organisms, together with the DIN calculated by the TapeStation software.

    Techniques: Generated, Sequencing, Next-Generation Sequencing, Software, Quantitation Assay, Marker

    Quantitation of Genomic DNA. Electropherogram ( a ) and gel image ( b ) of high molecular weight gDNA from Agilent 2200 TapeStation using the Genomic DNA ScreenTape System. Campylobacter ( green ), Listeria ( blue ), Vibrio ( aqua ), and Salmonella ( red ). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: Quantitation of Genomic DNA. Electropherogram ( a ) and gel image ( b ) of high molecular weight gDNA from Agilent 2200 TapeStation using the Genomic DNA ScreenTape System. Campylobacter ( green ), Listeria ( blue ), Vibrio ( aqua ), and Salmonella ( red ). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Article Snippet: The Agilent 2200 TapeStation System with the Genomic DNA assay was used to assess size and concentration of each sample as shown in Fig. , where an electropherogram overlay and virtual gel images are shown for the four model organisms, together with the DIN calculated by the TapeStation software.

    Techniques: Quantitation Assay, Molecular Weight, Marker

    Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to Tapestation bioanalyzer showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).

    Journal: PLoS ONE

    Article Title: Secretory microRNA-29 expression in gingival crevicular fluid during orthodontic tooth movement

    doi: 10.1371/journal.pone.0194238

    Figure Lengend Snippet: Presence of miRNA in healthy human crevicular fluid. “gel-like” images of R6K ScreenTape (A) after subjected to Tapestation bioanalyzer showed RNA ladder (L) on left lane and only small-sized RNA present in the sample lanes (middle and right lane). The positive bands approximately 50 nucleotides (nt) were present in representative GCF samples (A1 and B1). Electropherogram (B) corresponding to the gel-like images on the (A) figure. The x -axis on the electropherogram represents RNA size (nt), while the y -axis represents the measurement response of fluorescence units (FUs).

    Article Snippet: The quality and quantity of the extracted RNA was assessed using an Agilent 2200 Tapestation bioanalyzer (Agilent Technologies, Santa Clara, CA) and NanoDrop 1000 spectrophotometer (Thermoscientific, Waltham, MA).

    Techniques: Fluorescence

    Appearance of sheared DNA from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of sheared bacterial genomic DNA was generated with the Agilent 2200 TapeStation genomic DNA Kit with the average shearing size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: Appearance of sheared DNA from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of sheared bacterial genomic DNA was generated with the Agilent 2200 TapeStation genomic DNA Kit with the average shearing size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Article Snippet: The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes.

    Techniques: Generated, Quantitation Assay, Marker

    Appearance of DNA libraries from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of DNA libraries sizes (generated with the Agilent 2200 TapeStation DNA genomics Kit) prepared for sequencing with the PacBio SMRTbell 10kb Template Preparation Kit on the Agilent NGS Workstation. The average library size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb) is displayed on the software screen. Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: Appearance of DNA libraries from Agilent 2200 TapeStation analysis. Representative electropherogram ( a ) and virtual gel ( b ) of DNA libraries sizes (generated with the Agilent 2200 TapeStation DNA genomics Kit) prepared for sequencing with the PacBio SMRTbell 10kb Template Preparation Kit on the Agilent NGS Workstation. The average library size for Campylobacter ( green , 16 kb), Listeria ( blue , 12 kb), Vibrio ( aqua , 14 kb), and Salmonella ( red , 20 kb) is displayed on the software screen. Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Article Snippet: The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes.

    Techniques: Generated, Sequencing, Next-Generation Sequencing, Software, Quantitation Assay, Marker

    Quantitation of Genomic DNA. Electropherogram ( a ) and gel image ( b ) of high molecular weight gDNA from Agilent 2200 TapeStation using the Genomic DNA ScreenTape System. Campylobacter ( green ), Listeria ( blue ), Vibrio ( aqua ), and Salmonella ( red ). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: Quantitation of Genomic DNA. Electropherogram ( a ) and gel image ( b ) of high molecular weight gDNA from Agilent 2200 TapeStation using the Genomic DNA ScreenTape System. Campylobacter ( green ), Listeria ( blue ), Vibrio ( aqua ), and Salmonella ( red ). Green lines at the bottom of the gel image are internal standards added to permit quantitation. Lower marker is not shown in the electropherogram

    Article Snippet: The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes.

    Techniques: Quantitation Assay, Molecular Weight, Marker

    Comparison of electropherogram of gDNA using the Agilent 2200 TapeStation. gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Comparative Study of Seven Commercial Kits for Human DNA Extraction from Urine Samples Suitable for DNA Biomarker-Based Public Health Studies

    doi: 10.7171/jbt.14-2504-002

    Figure Lengend Snippet: Comparison of electropherogram of gDNA using the Agilent 2200 TapeStation. gDNA has been extracted from fresh urine samples of two female individuals using the Qv (blue, A, B), Qm (red, D, E) and iG (green, F, G) kits. TaqMan human gDNA control (C). L,

    Article Snippet: The integrity of gDNA was determined by the Agilent 2200 TapeStation Automated Electrophoresis System (Agilent Technologies, Waldbronn, Germany), according to the manufacturer's instructions.

    Techniques:

    Sampling time: comparison of electropherogram of gDNA using the Agilent 2200 TapeStation. gDNA has been extracted from two male (A) and 2 female (B) urine samples, collected at three different moments of the day: first morning urine (blue, A1, B1, C1,

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Comparative Study of Seven Commercial Kits for Human DNA Extraction from Urine Samples Suitable for DNA Biomarker-Based Public Health Studies

    doi: 10.7171/jbt.14-2504-002

    Figure Lengend Snippet: Sampling time: comparison of electropherogram of gDNA using the Agilent 2200 TapeStation. gDNA has been extracted from two male (A) and 2 female (B) urine samples, collected at three different moments of the day: first morning urine (blue, A1, B1, C1,

    Article Snippet: The integrity of gDNA was determined by the Agilent 2200 TapeStation Automated Electrophoresis System (Agilent Technologies, Waldbronn, Germany), according to the manufacturer's instructions.

    Techniques: Sampling