21983 Search Results


actinoplanes deccanensis atcc 21983  (ATCC)
94
ATCC actinoplanes deccanensis atcc 21983
Actinoplanes Deccanensis Atcc 21983, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
actinoplanes deccanensis atcc 21983 - by Bioz Stars, 2025-06
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rabbit anti tspan9  (Proteintech)
92
Proteintech rabbit anti tspan9
a , b Human monocytes were isolated from healthy donors. Human macrophages were induced with monocytes with human serum and MCSF. CAA plasma with or without Aβ40/Aβ42 removal or HC plasma was treated to monocytes/macrophages with a concentration of 10% in culture system for 6 h. a Immunolabeling of WGA to display migrasome production. Migrasomes of 30 cells in each group were subjected to the analysis. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). b Expression of TSPAN4 in monocytes/macrophages after plasma treatment as assessed with flow cytometry. Samples from 5 individuals in each group were subjected to the analysis. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). c Coronal brain sections from Tg-SwDI/B +/+ and 5xFAD mice or age- and sex-matched WT mice (24w of age) were collected and subjected to immunostaining of Iba1, Aβ40 and TSPAN4 and <t>TSPAN9.</t> Brain slices from 4 independent mice were subjected to the experiments. Representative images were displayed. d , e Tg-SwDI/B +/+ , Tg-SwDI/B +/- and WT mice were sacrificed at indicated age. ( d-i ) Plasma Aβ40 concentration was assessed with ELISA. d-ii, iii Leukocytes of mice were isolated and subjected to flow cytometric analysis of Aβ40 engulfment (CD45 + F4/80 + Aβ40 + ) d-ii and TSPAN4 expression (CD45 + F4/80 + TSPAN4 + ) in macrophages ( d-iii ). N = 3 in Tg-SwDI/B +/+ group, N = 3 in Tg-SwDI/B +/- group, N = 4 in WT group. Data are presented as mean values ± SEM with the indicated significance (compared with WT by one tail one-way ANOVA followed by Tukey’s post-test). e Comparison of TSPAN4 expression in CD45 + F4/80 + cells with (Aβ40 + ) or without (Aβ40 - ) Aβ40 engulfment at 12w of age. N = 3 in Tg-SwDI/B +/+ group, N = 3 in Tg-SwDI/B +/- group, N = 4 in WT group. Data are presented as mean values ± SEM with the indicated significance (compared with WT by two-tailed Student’s t test). Source data are provided as a Source Data file.
Rabbit Anti Tspan9, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti tspan9/product/Proteintech
Average 92 stars, based on 1 article reviews
rabbit anti tspan9 - by Bioz Stars, 2025-06
92/100 stars
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budvicia diplopodorum sp nov  (DSMZ)
85
DSMZ budvicia diplopodorum sp nov
a , b Human monocytes were isolated from healthy donors. Human macrophages were induced with monocytes with human serum and MCSF. CAA plasma with or without Aβ40/Aβ42 removal or HC plasma was treated to monocytes/macrophages with a concentration of 10% in culture system for 6 h. a Immunolabeling of WGA to display migrasome production. Migrasomes of 30 cells in each group were subjected to the analysis. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). b Expression of TSPAN4 in monocytes/macrophages after plasma treatment as assessed with flow cytometry. Samples from 5 individuals in each group were subjected to the analysis. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). c Coronal brain sections from Tg-SwDI/B +/+ and 5xFAD mice or age- and sex-matched WT mice (24w of age) were collected and subjected to immunostaining of Iba1, Aβ40 and TSPAN4 and <t>TSPAN9.</t> Brain slices from 4 independent mice were subjected to the experiments. Representative images were displayed. d , e Tg-SwDI/B +/+ , Tg-SwDI/B +/- and WT mice were sacrificed at indicated age. ( d-i ) Plasma Aβ40 concentration was assessed with ELISA. d-ii, iii Leukocytes of mice were isolated and subjected to flow cytometric analysis of Aβ40 engulfment (CD45 + F4/80 + Aβ40 + ) d-ii and TSPAN4 expression (CD45 + F4/80 + TSPAN4 + ) in macrophages ( d-iii ). N = 3 in Tg-SwDI/B +/+ group, N = 3 in Tg-SwDI/B +/- group, N = 4 in WT group. Data are presented as mean values ± SEM with the indicated significance (compared with WT by one tail one-way ANOVA followed by Tukey’s post-test). e Comparison of TSPAN4 expression in CD45 + F4/80 + cells with (Aβ40 + ) or without (Aβ40 - ) Aβ40 engulfment at 12w of age. N = 3 in Tg-SwDI/B +/+ group, N = 3 in Tg-SwDI/B +/- group, N = 4 in WT group. Data are presented as mean values ± SEM with the indicated significance (compared with WT by two-tailed Student’s t test). Source data are provided as a Source Data file.
Budvicia Diplopodorum Sp Nov, supplied by DSMZ, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
budvicia diplopodorum sp nov - by Bioz Stars, 2025-06
85/100 stars
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Image Search Results


a , b Human monocytes were isolated from healthy donors. Human macrophages were induced with monocytes with human serum and MCSF. CAA plasma with or without Aβ40/Aβ42 removal or HC plasma was treated to monocytes/macrophages with a concentration of 10% in culture system for 6 h. a Immunolabeling of WGA to display migrasome production. Migrasomes of 30 cells in each group were subjected to the analysis. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). b Expression of TSPAN4 in monocytes/macrophages after plasma treatment as assessed with flow cytometry. Samples from 5 individuals in each group were subjected to the analysis. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). c Coronal brain sections from Tg-SwDI/B +/+ and 5xFAD mice or age- and sex-matched WT mice (24w of age) were collected and subjected to immunostaining of Iba1, Aβ40 and TSPAN4 and TSPAN9. Brain slices from 4 independent mice were subjected to the experiments. Representative images were displayed. d , e Tg-SwDI/B +/+ , Tg-SwDI/B +/- and WT mice were sacrificed at indicated age. ( d-i ) Plasma Aβ40 concentration was assessed with ELISA. d-ii, iii Leukocytes of mice were isolated and subjected to flow cytometric analysis of Aβ40 engulfment (CD45 + F4/80 + Aβ40 + ) d-ii and TSPAN4 expression (CD45 + F4/80 + TSPAN4 + ) in macrophages ( d-iii ). N = 3 in Tg-SwDI/B +/+ group, N = 3 in Tg-SwDI/B +/- group, N = 4 in WT group. Data are presented as mean values ± SEM with the indicated significance (compared with WT by one tail one-way ANOVA followed by Tukey’s post-test). e Comparison of TSPAN4 expression in CD45 + F4/80 + cells with (Aβ40 + ) or without (Aβ40 - ) Aβ40 engulfment at 12w of age. N = 3 in Tg-SwDI/B +/+ group, N = 3 in Tg-SwDI/B +/- group, N = 4 in WT group. Data are presented as mean values ± SEM with the indicated significance (compared with WT by two-tailed Student’s t test). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

doi: 10.1038/s41467-023-39693-x

Figure Lengend Snippet: a , b Human monocytes were isolated from healthy donors. Human macrophages were induced with monocytes with human serum and MCSF. CAA plasma with or without Aβ40/Aβ42 removal or HC plasma was treated to monocytes/macrophages with a concentration of 10% in culture system for 6 h. a Immunolabeling of WGA to display migrasome production. Migrasomes of 30 cells in each group were subjected to the analysis. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). b Expression of TSPAN4 in monocytes/macrophages after plasma treatment as assessed with flow cytometry. Samples from 5 individuals in each group were subjected to the analysis. Data are presented as mean values ± SEM with the indicated significance (by one-way ANOVA followed by Tukey’s post-test). c Coronal brain sections from Tg-SwDI/B +/+ and 5xFAD mice or age- and sex-matched WT mice (24w of age) were collected and subjected to immunostaining of Iba1, Aβ40 and TSPAN4 and TSPAN9. Brain slices from 4 independent mice were subjected to the experiments. Representative images were displayed. d , e Tg-SwDI/B +/+ , Tg-SwDI/B +/- and WT mice were sacrificed at indicated age. ( d-i ) Plasma Aβ40 concentration was assessed with ELISA. d-ii, iii Leukocytes of mice were isolated and subjected to flow cytometric analysis of Aβ40 engulfment (CD45 + F4/80 + Aβ40 + ) d-ii and TSPAN4 expression (CD45 + F4/80 + TSPAN4 + ) in macrophages ( d-iii ). N = 3 in Tg-SwDI/B +/+ group, N = 3 in Tg-SwDI/B +/- group, N = 4 in WT group. Data are presented as mean values ± SEM with the indicated significance (compared with WT by one tail one-way ANOVA followed by Tukey’s post-test). e Comparison of TSPAN4 expression in CD45 + F4/80 + cells with (Aβ40 + ) or without (Aβ40 - ) Aβ40 engulfment at 12w of age. N = 3 in Tg-SwDI/B +/+ group, N = 3 in Tg-SwDI/B +/- group, N = 4 in WT group. Data are presented as mean values ± SEM with the indicated significance (compared with WT by two-tailed Student’s t test). Source data are provided as a Source Data file.

Article Snippet: The following primary antibodies were used: rabbit anti-TSPAN4 (Abcam ab181995, 1:200), rabbit anti-TSPAN7 (Proteintech 18695-1-AP, 1:200), rabbit anti-Tspan9 (Proteintech 21983-1-AP, 1:200), rabbit anti-NDST1 (Proteintech 26203-1-AP, 1:200).

Techniques: Isolation, Concentration Assay, Immunolabeling, Expressing, Flow Cytometry, Immunostaining, Enzyme-linked Immunosorbent Assay, Two Tailed Test