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  • 99
    Agilent technologies 2100 bioanalyzer instrument
    Depletion of rRNA from RNA samples isolated from dual species P. aeruginosa / S. aureus cultures. A total of 2 μg of DNAse-treated RNA, isolated form a dual species P. aeruginosa PAO1 and S. aureus ATCC6538 culture, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the <t>Bioanalyzer</t> RNA 6000 Pico kit. Representative electropherograms of ( A ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( B ) MICROBExpress or ( C ) Ribo-Zero kits are shown. ( D ) Percent of rRNA contamination as reported by the Agilent Bioanalyzer <t>2100</t> Expert Software in RNA samples from single and dual species cultures of P. aeruginosa and/or S. aureus treated with the Ribo-Zero or MICROBExpress kits. Untreated input RNA (Total) or RNA processed via Ribo-Zero or MICROBExpress treatment was subjected to qPCR analysis of P. aeruginosa ( E , G ) or S. aureus ( F , H ) rRNA transcript abundance. ( E , F ) qPCR threshold cycle (Cq) for the indicated rRNA transcripts. ( G , H ) Copy numbers of P. aeruginosa or S. aureus 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.
    2100 Bioanalyzer Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 6814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer instrument/product/Agilent technologies
    Average 99 stars, based on 6814 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer instrument - by Bioz Stars, 2019-09
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies 2100 bioanalyser system
    Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent <t>2100</t> <t>bioanalyser</t> system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.
    2100 Bioanalyser System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyser system/product/Agilent technologies
    Average 99 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyser system - by Bioz Stars, 2019-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Depletion of rRNA from RNA samples isolated from dual species P. aeruginosa / S. aureus cultures. A total of 2 μg of DNAse-treated RNA, isolated form a dual species P. aeruginosa PAO1 and S. aureus ATCC6538 culture, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( A ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( B ) MICROBExpress or ( C ) Ribo-Zero kits are shown. ( D ) Percent of rRNA contamination as reported by the Agilent Bioanalyzer 2100 Expert Software in RNA samples from single and dual species cultures of P. aeruginosa and/or S. aureus treated with the Ribo-Zero or MICROBExpress kits. Untreated input RNA (Total) or RNA processed via Ribo-Zero or MICROBExpress treatment was subjected to qPCR analysis of P. aeruginosa ( E , G ) or S. aureus ( F , H ) rRNA transcript abundance. ( E , F ) qPCR threshold cycle (Cq) for the indicated rRNA transcripts. ( G , H ) Copy numbers of P. aeruginosa or S. aureus 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.

    Journal: Scientific Reports

    Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes

    doi: 10.1038/srep41114

    Figure Lengend Snippet: Depletion of rRNA from RNA samples isolated from dual species P. aeruginosa / S. aureus cultures. A total of 2 μg of DNAse-treated RNA, isolated form a dual species P. aeruginosa PAO1 and S. aureus ATCC6538 culture, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( A ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( B ) MICROBExpress or ( C ) Ribo-Zero kits are shown. ( D ) Percent of rRNA contamination as reported by the Agilent Bioanalyzer 2100 Expert Software in RNA samples from single and dual species cultures of P. aeruginosa and/or S. aureus treated with the Ribo-Zero or MICROBExpress kits. Untreated input RNA (Total) or RNA processed via Ribo-Zero or MICROBExpress treatment was subjected to qPCR analysis of P. aeruginosa ( E , G ) or S. aureus ( F , H ) rRNA transcript abundance. ( E , F ) qPCR threshold cycle (Cq) for the indicated rRNA transcripts. ( G , H ) Copy numbers of P. aeruginosa or S. aureus 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.

    Article Snippet: RNA quantity and quality post-depletion was assessed using the Agilent 2100 Bioanalyzer instrument as described above.

    Techniques: Isolation, Software, Real-time Polymerase Chain Reaction, Standard Deviation

    Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.

    Journal: Scientific Reports

    Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes

    doi: 10.1038/srep41114

    Figure Lengend Snippet: Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.

    Article Snippet: RNA quantity and quality post-depletion was assessed using the Agilent 2100 Bioanalyzer instrument as described above.

    Techniques: Isolation, Quantitation Assay, RNA HS Assay, Software, Fluorescence

    Quality and quantity of biomacromolecular fractions isolated from the representative LAO-enriched microbial community sample using either the NA-, QA- and TR-based method (following prior metabolite extractions) or using the reference methods (no metabolite extractions were carried out before the respective extractions). ( a and b ) Representative Agilent Bioanalyzer 2100 electropherograms of the total RNA and small RNA fractions, respectively. ( c ) Agarose gel image highlighting representative genomic DNA fractions obtained (Mean amount ( n =3) loaded in μg±s.d., from right to left; NA: 0.35±0.17; QA: 0.35±0.08; TR: 0.83±0.85; RM: 0.08±0.04) and ( d ) SDS-PAGE image of representative protein fractions (Mean amount ( n =3) loaded in μg±s.d., from right to left; NA, first elution: 3.20±0.19; QA: 5.44±1.06; TR: 3.88±0.30; RM: 4.62±0.09). The arrow and the box represent the dominant gel band which was submitted to tryptic digestion and MALDI-ToF/ToF analysis. ( e ) Biomacromolecular yield obtained for the small RNA, □ total and large RNA, DNA and protein (first elution) fractions ( n =5, error bars represent s.d.). FU, fluorescent unit; L, ladder; M, marker; NA, NA-based method; Norm., normalized; nt, nucleotides; RM, reference methods; TR, TR-based method; QA, QA-based method.

    Journal: The ISME Journal

    Article Title: A biomolecular isolation framework for eco-systems biology

    doi: 10.1038/ismej.2012.72

    Figure Lengend Snippet: Quality and quantity of biomacromolecular fractions isolated from the representative LAO-enriched microbial community sample using either the NA-, QA- and TR-based method (following prior metabolite extractions) or using the reference methods (no metabolite extractions were carried out before the respective extractions). ( a and b ) Representative Agilent Bioanalyzer 2100 electropherograms of the total RNA and small RNA fractions, respectively. ( c ) Agarose gel image highlighting representative genomic DNA fractions obtained (Mean amount ( n =3) loaded in μg±s.d., from right to left; NA: 0.35±0.17; QA: 0.35±0.08; TR: 0.83±0.85; RM: 0.08±0.04) and ( d ) SDS-PAGE image of representative protein fractions (Mean amount ( n =3) loaded in μg±s.d., from right to left; NA, first elution: 3.20±0.19; QA: 5.44±1.06; TR: 3.88±0.30; RM: 4.62±0.09). The arrow and the box represent the dominant gel band which was submitted to tryptic digestion and MALDI-ToF/ToF analysis. ( e ) Biomacromolecular yield obtained for the small RNA, □ total and large RNA, DNA and protein (first elution) fractions ( n =5, error bars represent s.d.). FU, fluorescent unit; L, ladder; M, marker; NA, NA-based method; Norm., normalized; nt, nucleotides; RM, reference methods; TR, TR-based method; QA, QA-based method.

    Article Snippet: The Agilent Bioanalyzer 2100 electropherograms ( ) show distinct peaks between 100 and 4500 nt representing the total RNA fractions obtained using the different methods.

    Techniques: Isolation, Agarose Gel Electrophoresis, SDS Page, Marker

    Application of the developed biomolecular isolation methodology to a LAO-enriched microbial community ( n =3), river water filtrate ( n =3) and human feces ( n =3). ( a – c ) Left-hand panels represent LAO-enriched microbial communities, middle panels represent river water filtrate and right-hand panels represent human feces. ( a ) Representative GC–MS total ion chromatograms of polar and non-polar metabolite fractions. Representative Agilent Bioanalyzer 2100 electropherograms of the ( b ) total RNA fractions and ( c ) small RNA fractions. ( d ) Agarose gel electrophoresis image of the genomic DNA fractions (Mean amount loaded in μg±s.d., right to left; LAO: 0.63±0.28; river water: 0.35±0.03; feces: 0.61±0.26) for each of the three technical replicates considered. ( e ) SDS-PAGE image of protein fractions, first elution (Mean amount loaded in μg±s.d., right to left; LAO: 1.19±0.40; river water: 1.70±0.63; feces: 1.19±1.21) for each of the three technical replicates considered. L, ladder; RT, retention time.

    Journal: The ISME Journal

    Article Title: A biomolecular isolation framework for eco-systems biology

    doi: 10.1038/ismej.2012.72

    Figure Lengend Snippet: Application of the developed biomolecular isolation methodology to a LAO-enriched microbial community ( n =3), river water filtrate ( n =3) and human feces ( n =3). ( a – c ) Left-hand panels represent LAO-enriched microbial communities, middle panels represent river water filtrate and right-hand panels represent human feces. ( a ) Representative GC–MS total ion chromatograms of polar and non-polar metabolite fractions. Representative Agilent Bioanalyzer 2100 electropherograms of the ( b ) total RNA fractions and ( c ) small RNA fractions. ( d ) Agarose gel electrophoresis image of the genomic DNA fractions (Mean amount loaded in μg±s.d., right to left; LAO: 0.63±0.28; river water: 0.35±0.03; feces: 0.61±0.26) for each of the three technical replicates considered. ( e ) SDS-PAGE image of protein fractions, first elution (Mean amount loaded in μg±s.d., right to left; LAO: 1.19±0.40; river water: 1.70±0.63; feces: 1.19±1.21) for each of the three technical replicates considered. L, ladder; RT, retention time.

    Article Snippet: The Agilent Bioanalyzer 2100 electropherograms ( ) show distinct peaks between 100 and 4500 nt representing the total RNA fractions obtained using the different methods.

    Techniques: Isolation, Gas Chromatography, Mass Spectrometry, Agarose Gel Electrophoresis, Electrophoresis, SDS Page

    Analysis of tear proteins obtained by running aliquots from the same samples in parallel. Tear samples (one to eight) were runned through the 2100 Bioanalyzer (upper part of the figure, Protein 230 kit, gel-like view), and the 1D SDS–PAGE (lower part of the figure). The corresponding molecular weight in kDa is reported on the left of the ladder L : and of the prestained protein standard S : The same profiles were obtained for each sample in the two analytical methods.

    Journal: Molecular Vision

    Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins

    doi:

    Figure Lengend Snippet: Analysis of tear proteins obtained by running aliquots from the same samples in parallel. Tear samples (one to eight) were runned through the 2100 Bioanalyzer (upper part of the figure, Protein 230 kit, gel-like view), and the 1D SDS–PAGE (lower part of the figure). The corresponding molecular weight in kDa is reported on the left of the ladder L : and of the prestained protein standard S : The same profiles were obtained for each sample in the two analytical methods.

    Article Snippet: The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data.

    Techniques: SDS Page, Molecular Weight

    Inter-practitioner (Techn1 and Techn2) measurement variability (reproducibility) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed by Techn1 (y-axis) and by Techn 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed by two different laboratory technicians (Techn 1 and Techn 2), is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained by Techn 1 and Techn 2. The y-axis indicates the difference in measurement between the two technicians. The mean difference of the technicians is 0.01, with an upper specification limit of 0.23 and a lower specification limit of 0.20. C : The concordance correlation coefficients calculated for the two technicians are shown; all values demonstrated the high reproducibility of the Bioanalyzer method.

    Journal: Molecular Vision

    Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins

    doi:

    Figure Lengend Snippet: Inter-practitioner (Techn1 and Techn2) measurement variability (reproducibility) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed by Techn1 (y-axis) and by Techn 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed by two different laboratory technicians (Techn 1 and Techn 2), is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained by Techn 1 and Techn 2. The y-axis indicates the difference in measurement between the two technicians. The mean difference of the technicians is 0.01, with an upper specification limit of 0.23 and a lower specification limit of 0.20. C : The concordance correlation coefficients calculated for the two technicians are shown; all values demonstrated the high reproducibility of the Bioanalyzer method.

    Article Snippet: The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data.

    Techniques:

    Degree of agreement between measurements conducted on replicate specimens. The same tear samples were runned with two methods: monodimensional sodium dodecyl sulfate-polyacrylamide lectrophoresis (1D SDS–PAGE) electrophoresis and the 2100 Agilent Bioanalyzer (Protein 230 kit). A : The measurements obtained with 1D-SDS–PAGE electrophoresis (y-axis) and the 2100 Agilent Bioanalyzer (x-axis) are graphed. B : The Bland–Altman plot for various samples of tears analyzed for lysozyme content, performed with the two methods, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained with 1D SDS–PAGE electrophoresis and the 2100 Agilent Bioanalyzer. The y-axis indicates the difference in measurement between the two methods. The mean difference is 0.08, with an upper specification limit of 0.28 and a lower specification limit of 0.43. C : The concordance correlation coefficients calculated for the two methods are shown; all values demonstrated high correlation between the two methods.

    Journal: Molecular Vision

    Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins

    doi:

    Figure Lengend Snippet: Degree of agreement between measurements conducted on replicate specimens. The same tear samples were runned with two methods: monodimensional sodium dodecyl sulfate-polyacrylamide lectrophoresis (1D SDS–PAGE) electrophoresis and the 2100 Agilent Bioanalyzer (Protein 230 kit). A : The measurements obtained with 1D-SDS–PAGE electrophoresis (y-axis) and the 2100 Agilent Bioanalyzer (x-axis) are graphed. B : The Bland–Altman plot for various samples of tears analyzed for lysozyme content, performed with the two methods, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained with 1D SDS–PAGE electrophoresis and the 2100 Agilent Bioanalyzer. The y-axis indicates the difference in measurement between the two methods. The mean difference is 0.08, with an upper specification limit of 0.28 and a lower specification limit of 0.43. C : The concordance correlation coefficients calculated for the two methods are shown; all values demonstrated high correlation between the two methods.

    Article Snippet: The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data.

    Techniques: SDS Page, Electrophoresis

    Inter-session (session 1 and session 2) measurement variability (repeatability) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed during session 1 (y-axis) and session 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed in two sessions, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained in session 1 and session 2. The y-axis indicates the difference in measurement between the two sessions. The mean difference of both sessions is 0.04, with an upper specification limit of 0.24 and a lower specification limit of 0.16. C : The concordance correlation coefficients calculated for the two sessions are shown; all values demonstrated the high repeatability of the Bioanalyzer method.

    Journal: Molecular Vision

    Article Title: A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins

    doi:

    Figure Lengend Snippet: Inter-session (session 1 and session 2) measurement variability (repeatability) for the Agilent 2100 Bioanalyzer is shown. A : The tear lysozyme measurements (mg/ml) performed during session 1 (y-axis) and session 2 (x-axis) on the same tear samples are graphed. B : The Bland–Altman plot for various tear samples analyzed for lysozyme content, performed in two sessions, is shown. The x-axis indicates the mean of the lysozyme content in mg/ml obtained in session 1 and session 2. The y-axis indicates the difference in measurement between the two sessions. The mean difference of both sessions is 0.04, with an upper specification limit of 0.24 and a lower specification limit of 0.16. C : The concordance correlation coefficients calculated for the two sessions are shown; all values demonstrated the high repeatability of the Bioanalyzer method.

    Article Snippet: The Agilent 2100 Bioanalyzer system included protein assay analysis software and software for managing patient data.

    Techniques:

    A representation of the gel image generated by the Agilent 2100 Bioanalyzer for multiplex PCR Serotyping method of Salmonella enterica serovar Typhimurium in Horse Feed (HF), Whole Oats (OT), Dried Beet Pulp (DBP), Calf Milk Replacer (CMR), Dried Molasses (DM) and Wheat Brand (WB) spiked with 10 CFU/25 g, 50 CFU/25 g and 100 CFU/25 g. Fig. 1 a–f (Lane 1) Ladder, (Lane 2–4) 100 CFU/25 g of reaction 1–3, (Lane 5–7) 50 CFU/25 g of reaction 1–3, (Lane 8–10) 10 CFU/25 g of reaction 1–3. Figure a is for HF, Figure b is for OT, Figure c is for DM, Figure d is for WB, Figure e is for CMR, and Figure f is for DBP.

    Journal: MethodsX

    Article Title: Evaluation of a multiplex PCR method to serotype Salmonella in animal feeds pre-enrichment broth cultures

    doi: 10.1016/j.mex.2017.09.003

    Figure Lengend Snippet: A representation of the gel image generated by the Agilent 2100 Bioanalyzer for multiplex PCR Serotyping method of Salmonella enterica serovar Typhimurium in Horse Feed (HF), Whole Oats (OT), Dried Beet Pulp (DBP), Calf Milk Replacer (CMR), Dried Molasses (DM) and Wheat Brand (WB) spiked with 10 CFU/25 g, 50 CFU/25 g and 100 CFU/25 g. Fig. 1 a–f (Lane 1) Ladder, (Lane 2–4) 100 CFU/25 g of reaction 1–3, (Lane 5–7) 50 CFU/25 g of reaction 1–3, (Lane 8–10) 10 CFU/25 g of reaction 1–3. Figure a is for HF, Figure b is for OT, Figure c is for DM, Figure d is for WB, Figure e is for CMR, and Figure f is for DBP.

    Article Snippet: • Vitek® 2 Compact, Version 5, (Biomerieux, St Louis, MO) • Roche MagNA Pure Compact (Roche, Indianapolis IN) • BioRad conventional C1000 thermocycler (BioRad, Hercules, CA) • Eppendorf Centrifuge 5424R (Eppendorf, Hauppauge, NY) Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) • DNA 1000 Reagents kit for DNA analysis (Agilent Technologies, Waldbronn, Germany) • PCR tubes (BioRad, Hercules, CA)) • Sterile Eppendorf style microcentrifuge tubes ((Life Sciences, Hercules, CA or equivalent) • Sterile inoculating loops or needles (Life Sciences, Hercules, CA or equivalent) • Ice bucket or bench top cooler • Adjustable Micropipettors (0.1–1000 μl) • Aerosol resistant micropipettor tips (0.1–1000 μl) • Vortex Mixer (Life Sciences, or equivalent) • Microcentrifuge tips (Life Sciences, Hercules, CA or equivalent)

    Techniques: Generated, Multiplex Assay, Polymerase Chain Reaction, Western Blot

    Serotyping reaction 1, and 2 demonstrated in the Agilent 2100 Bioanalyzer gel image for the various Salmonella enterica serovars: (Lane 1) Ladder; (Lane 2–3) Typhimurium from Horse Feed with serotypic banding pattern ABCDEI; (Lane 4–5) Agona for Whole Oats with serotypic banding pattern BCJ; (Lane 6–7) Hadar for Calf Milk Replacer with serotypic banding pattern BC.

    Journal: MethodsX

    Article Title: Evaluation of a multiplex PCR method to serotype Salmonella in animal feeds pre-enrichment broth cultures

    doi: 10.1016/j.mex.2017.09.003

    Figure Lengend Snippet: Serotyping reaction 1, and 2 demonstrated in the Agilent 2100 Bioanalyzer gel image for the various Salmonella enterica serovars: (Lane 1) Ladder; (Lane 2–3) Typhimurium from Horse Feed with serotypic banding pattern ABCDEI; (Lane 4–5) Agona for Whole Oats with serotypic banding pattern BCJ; (Lane 6–7) Hadar for Calf Milk Replacer with serotypic banding pattern BC.

    Article Snippet: • Vitek® 2 Compact, Version 5, (Biomerieux, St Louis, MO) • Roche MagNA Pure Compact (Roche, Indianapolis IN) • BioRad conventional C1000 thermocycler (BioRad, Hercules, CA) • Eppendorf Centrifuge 5424R (Eppendorf, Hauppauge, NY) Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) • DNA 1000 Reagents kit for DNA analysis (Agilent Technologies, Waldbronn, Germany) • PCR tubes (BioRad, Hercules, CA)) • Sterile Eppendorf style microcentrifuge tubes ((Life Sciences, Hercules, CA or equivalent) • Sterile inoculating loops or needles (Life Sciences, Hercules, CA or equivalent) • Ice bucket or bench top cooler • Adjustable Micropipettors (0.1–1000 μl) • Aerosol resistant micropipettor tips (0.1–1000 μl) • Vortex Mixer (Life Sciences, or equivalent) • Microcentrifuge tips (Life Sciences, Hercules, CA or equivalent)

    Techniques:

    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Journal:

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Journal:

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Journal:

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Journal:

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Journal:

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques: Fluorescence

    Transcriptional changes of cir gene expression during the course of infection . For RFLP analyses of expression changes of the cir genes during the course of infection, blood of female NMRI mice infected with 100 pRBCs were passaged on days 7, 14 and 21 days post infections (d.p.i.) into naïve female NMRI mice. Blood of these passaged mice was again collected at 30% parasitaemia. After amplification using the subfamily-specific primers for both subfamilies, 150 ng of purified RT-PCR products (approx. 600 bp) were digested with Alu I and 30 ng were then analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer. The RT-PCR RFLP profiles for four mice of subfamily 1 are presented in (A). The restriction digest of subfamily 2 are shown in the upper panel of (B) where only at day 7 p.i. and day 14 p.i. of mouse 1 a few smaller restriction fragments could be detected. Therefore a second digest with Xap I for 3 h at 37°C was performed (B, lower panel). DNA 1000 bp ladder was used and in each lane an upper (1000 bp, purple) and a lower (25 bp, green) marker are indicated.

    Journal: Malaria Journal

    Article Title: Characterization and tissue-specific expression patterns of the Plasmodium chabaudi cir multigene family

    doi: 10.1186/1475-2875-10-272

    Figure Lengend Snippet: Transcriptional changes of cir gene expression during the course of infection . For RFLP analyses of expression changes of the cir genes during the course of infection, blood of female NMRI mice infected with 100 pRBCs were passaged on days 7, 14 and 21 days post infections (d.p.i.) into naïve female NMRI mice. Blood of these passaged mice was again collected at 30% parasitaemia. After amplification using the subfamily-specific primers for both subfamilies, 150 ng of purified RT-PCR products (approx. 600 bp) were digested with Alu I and 30 ng were then analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer. The RT-PCR RFLP profiles for four mice of subfamily 1 are presented in (A). The restriction digest of subfamily 2 are shown in the upper panel of (B) where only at day 7 p.i. and day 14 p.i. of mouse 1 a few smaller restriction fragments could be detected. Therefore a second digest with Xap I for 3 h at 37°C was performed (B, lower panel). DNA 1000 bp ladder was used and in each lane an upper (1000 bp, purple) and a lower (25 bp, green) marker are indicated.

    Article Snippet: After amplification using the subfamily 1 and subfamily 2 specific primers, PCR products were digested with the restriction enzyme Alu I and analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer for accurate and reproducible separation and size determination (Figure ).

    Techniques: Expressing, Infection, Mouse Assay, Amplification, Purification, Reverse Transcription Polymerase Chain Reaction, Marker

    Expression profile analyses of cir genes in different tissues of infected mice by RT-PCR RFLP . For RFLP analyses, organs and blood were collected at a parasitaemia of 30% from female NMRI mice infected with 100 pRBCs. After RT-PCR amplification for the six host tissues with the subfamily-specific primers, 150 ng of purified RT-PCR products (approx. 600 bp) were digested with Alu I. The DNA fragments (30 ng) were analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer for accurate sizing. Compared are RT-PCR RFLP profiles of blood, liver, spleen, kidney, lung and brain for four mice for cir subfamily 1 (A) and cir subfamily 2 (B). DNA 1000 bp ladder was used and in each lane an upper (1000 bp, purple) and a lower (25 bp, green) marker are indicated.

    Journal: Malaria Journal

    Article Title: Characterization and tissue-specific expression patterns of the Plasmodium chabaudi cir multigene family

    doi: 10.1186/1475-2875-10-272

    Figure Lengend Snippet: Expression profile analyses of cir genes in different tissues of infected mice by RT-PCR RFLP . For RFLP analyses, organs and blood were collected at a parasitaemia of 30% from female NMRI mice infected with 100 pRBCs. After RT-PCR amplification for the six host tissues with the subfamily-specific primers, 150 ng of purified RT-PCR products (approx. 600 bp) were digested with Alu I. The DNA fragments (30 ng) were analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer for accurate sizing. Compared are RT-PCR RFLP profiles of blood, liver, spleen, kidney, lung and brain for four mice for cir subfamily 1 (A) and cir subfamily 2 (B). DNA 1000 bp ladder was used and in each lane an upper (1000 bp, purple) and a lower (25 bp, green) marker are indicated.

    Article Snippet: After amplification using the subfamily 1 and subfamily 2 specific primers, PCR products were digested with the restriction enzyme Alu I and analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer for accurate and reproducible separation and size determination (Figure ).

    Techniques: Expressing, Infection, Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Amplification, Purification, Marker

    Transcriptional changes of cir genes during intraerythrocytic development . For expression profiling of the cir genes at three different time points in the life cycle, 30 μl tail vein blood of female NMRI mice infected with 100 pRBCs were collected 3 h (early trophozoites), 10 h (late trophozoites) and 17 h (mature trophozoites and early schizonts) after beginning of the light cycle on day 13 p.i. (parasitaemia about 30%). Amplification was performed using the subfamily-specific primers for both subfamilies and 150 ng of purified RT-PCR products (approx. 600 bp) were digested with Alu I and 30 ng were then analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer. The RFLP profiles of subfamily 1 are shown for four mice in (A). The restriction digests of subfamily 2 are shown in the upper panel of (B). Only a few restriction fragments were detected in all samples, therefore RT-PCR products were also restricted with Xap I (B, lower panel). DNA 1000 bp ladder was used and in each lane an upper (1000 bp, purple) and a lower (25 bp, green) marker were indicated.

    Journal: Malaria Journal

    Article Title: Characterization and tissue-specific expression patterns of the Plasmodium chabaudi cir multigene family

    doi: 10.1186/1475-2875-10-272

    Figure Lengend Snippet: Transcriptional changes of cir genes during intraerythrocytic development . For expression profiling of the cir genes at three different time points in the life cycle, 30 μl tail vein blood of female NMRI mice infected with 100 pRBCs were collected 3 h (early trophozoites), 10 h (late trophozoites) and 17 h (mature trophozoites and early schizonts) after beginning of the light cycle on day 13 p.i. (parasitaemia about 30%). Amplification was performed using the subfamily-specific primers for both subfamilies and 150 ng of purified RT-PCR products (approx. 600 bp) were digested with Alu I and 30 ng were then analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer. The RFLP profiles of subfamily 1 are shown for four mice in (A). The restriction digests of subfamily 2 are shown in the upper panel of (B). Only a few restriction fragments were detected in all samples, therefore RT-PCR products were also restricted with Xap I (B, lower panel). DNA 1000 bp ladder was used and in each lane an upper (1000 bp, purple) and a lower (25 bp, green) marker were indicated.

    Article Snippet: After amplification using the subfamily 1 and subfamily 2 specific primers, PCR products were digested with the restriction enzyme Alu I and analysed using the DNA 1000 kit for the Agilent 2100 bioanalyzer for accurate and reproducible separation and size determination (Figure ).

    Techniques: Expressing, Mouse Assay, Infection, Amplification, Purification, Reverse Transcription Polymerase Chain Reaction, Marker

    Size distribution of mRNA, cRNA and dsDNA on Agilent 2100 Bioanalyzer 6000 Nanochips. ( a ) Universal Human Reference (UHR) RNA and sense-RNA template library after IVT-amplification. Lane L displays the ladder (25, 200, 500, 1000, 2000 and 4000 nt). Lane 1 contains fresh UHR RNA. Lanes 2, 3 and 4 display three individual IVT-amplifications of sense-RNA using total RNA displayed in lane 1. ( b ) Size distribution of fresh, frozen, FFPE-RNA and amplified cRNA. Lane L displays the same ladder as observed in (a). Lane 1 contains fresh human breast RNA. Lane 2 contains total RNA from the 10-year-old frozen human breast cancer tissue. Lane 3 contains total RNA from the matched 10-year-old FFPE human breast cancer tissue. Lane 4 contains cRNA obtained by IVT-amplifications of 10-year-old frozen RNA (lane 2). Lanes 5 contains cRNA obtained by direct IVT-amplification of the 10-year-old FFPE-RNA (lane 3). Lanes 6 contains amplified cRNA obtained by CT-RT and IVT-amplification of the same 10-year-old FFPE-RNA. ( c ) Size distribution of double-stranded DNA on a Bioanalyzer 2100 Agilent nanochip. Lane L displays the ladder. Lane 1 displays dsDNA obtained from 10-year-old frozen RNA. Lane 2 displays dsDNA obtained from 10-year-old FFPE-RNA. Lane 3 shows dsDNA obtained after CT-RT and double-strand DNA synthesis of the same 10-year-old FFPE-RNA. (See Supplementary Data for fragmented RNA profiles)

    Journal: Nucleic Acids Research

    Article Title: Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)

    doi: 10.1093/nar/gkm510

    Figure Lengend Snippet: Size distribution of mRNA, cRNA and dsDNA on Agilent 2100 Bioanalyzer 6000 Nanochips. ( a ) Universal Human Reference (UHR) RNA and sense-RNA template library after IVT-amplification. Lane L displays the ladder (25, 200, 500, 1000, 2000 and 4000 nt). Lane 1 contains fresh UHR RNA. Lanes 2, 3 and 4 display three individual IVT-amplifications of sense-RNA using total RNA displayed in lane 1. ( b ) Size distribution of fresh, frozen, FFPE-RNA and amplified cRNA. Lane L displays the same ladder as observed in (a). Lane 1 contains fresh human breast RNA. Lane 2 contains total RNA from the 10-year-old frozen human breast cancer tissue. Lane 3 contains total RNA from the matched 10-year-old FFPE human breast cancer tissue. Lane 4 contains cRNA obtained by IVT-amplifications of 10-year-old frozen RNA (lane 2). Lanes 5 contains cRNA obtained by direct IVT-amplification of the 10-year-old FFPE-RNA (lane 3). Lanes 6 contains amplified cRNA obtained by CT-RT and IVT-amplification of the same 10-year-old FFPE-RNA. ( c ) Size distribution of double-stranded DNA on a Bioanalyzer 2100 Agilent nanochip. Lane L displays the ladder. Lane 1 displays dsDNA obtained from 10-year-old frozen RNA. Lane 2 displays dsDNA obtained from 10-year-old FFPE-RNA. Lane 3 shows dsDNA obtained after CT-RT and double-strand DNA synthesis of the same 10-year-old FFPE-RNA. (See Supplementary Data for fragmented RNA profiles)

    Article Snippet: For these experiments, total RNA isolated from the 3-year-old cervical frozen and formalin-fixed tissues, as well as the cRNA obtained by IVT-amplification of frozen RNA or by CT-RT and IVT-ampliication of FFPE-RNA were analyzed on the Agilent 2100 bioanalyzer (Supplementary Data).

    Techniques: Amplification, Formalin-fixed Paraffin-Embedded, DNA Synthesis

    Electropherogram of total RNA recovered from FFPE samples utilized in CT-RT experiments. Total RNA extracted from 10-year-old breast cancer and 3-year-old cervical FFPE tissues were loaded on a Agilent 2100 Bioanalyzer 6000 Nanochip. Electropherograms were obtained with the 2100 expert software from Agilent, version B.02.03.SI307. 18S and 28S ribosomal RNA peaks are not detected in either FFPE sample, indicative of severe degradation.

    Journal: Nucleic Acids Research

    Article Title: Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)

    doi: 10.1093/nar/gkm510

    Figure Lengend Snippet: Electropherogram of total RNA recovered from FFPE samples utilized in CT-RT experiments. Total RNA extracted from 10-year-old breast cancer and 3-year-old cervical FFPE tissues were loaded on a Agilent 2100 Bioanalyzer 6000 Nanochip. Electropherograms were obtained with the 2100 expert software from Agilent, version B.02.03.SI307. 18S and 28S ribosomal RNA peaks are not detected in either FFPE sample, indicative of severe degradation.

    Article Snippet: For these experiments, total RNA isolated from the 3-year-old cervical frozen and formalin-fixed tissues, as well as the cRNA obtained by IVT-amplification of frozen RNA or by CT-RT and IVT-ampliication of FFPE-RNA were analyzed on the Agilent 2100 bioanalyzer (Supplementary Data).

    Techniques: Formalin-fixed Paraffin-Embedded, Software

    Standards for MspI digestion and progressive PCR. A) MspI digestion of human genomic DNA isolated from human post-mortem brain tissues. DNA (200 ng) was digested by MspI and run on a 4–20% precast polyacrylamide gel and stained with EtBr. Arrows show three satellite DNA bands characteristic of this enzymatic digestion. B) Agilent 2100 Bioanalyzer chromatogram of MspI digested genomic DNA. C) Bioanalyzer 2100 image of a single library from an MspI digested DNA sample. Notice that the satellite bands (indicated by arrows) are still visible on the Bioanalyzer image. D) Progressive PCR amplification combined with limited PCR extension time allows for size selection and amplification of six bisulfite converted libraries (Lanes 1–5 are distinct RRBS libraries; lane 6 (‘C’) is a negative control). After different progressive PCR cycles (18X, 22X, 24X, or 26X – the same libraries are shown for each cycle number) band intensity increases as cycle number increases. Arrows indicate the three satellite DNA bands that are still visible in these libraries.

    Journal: BMC Genomics

    Article Title: BisQC: an operational pipeline for multiplexed bisulfite sequencing

    doi: 10.1186/1471-2164-15-290

    Figure Lengend Snippet: Standards for MspI digestion and progressive PCR. A) MspI digestion of human genomic DNA isolated from human post-mortem brain tissues. DNA (200 ng) was digested by MspI and run on a 4–20% precast polyacrylamide gel and stained with EtBr. Arrows show three satellite DNA bands characteristic of this enzymatic digestion. B) Agilent 2100 Bioanalyzer chromatogram of MspI digested genomic DNA. C) Bioanalyzer 2100 image of a single library from an MspI digested DNA sample. Notice that the satellite bands (indicated by arrows) are still visible on the Bioanalyzer image. D) Progressive PCR amplification combined with limited PCR extension time allows for size selection and amplification of six bisulfite converted libraries (Lanes 1–5 are distinct RRBS libraries; lane 6 (‘C’) is a negative control). After different progressive PCR cycles (18X, 22X, 24X, or 26X – the same libraries are shown for each cycle number) band intensity increases as cycle number increases. Arrows indicate the three satellite DNA bands that are still visible in these libraries.

    Article Snippet: AMPure SPRI bead-purified library was eluted in 60 μL of dH2 O. Purified libraries can be screened on an Agilent 2100 BioAnalyzer (Figure A and B).

    Techniques: Polymerase Chain Reaction, Isolation, Staining, Amplification, Selection, Negative Control

    Agilent 2100 Bioanalyzer images from final reduced representation bisulfite libraries. A) High Sensitivity DNA Chip from 10 RRBS libraries. Notice that the satellite bands are still visible on the Bioanalyzer gel image B) Chromatogram representation of panel A showing high quality RRBS libraries.

    Journal: BMC Genomics

    Article Title: BisQC: an operational pipeline for multiplexed bisulfite sequencing

    doi: 10.1186/1471-2164-15-290

    Figure Lengend Snippet: Agilent 2100 Bioanalyzer images from final reduced representation bisulfite libraries. A) High Sensitivity DNA Chip from 10 RRBS libraries. Notice that the satellite bands are still visible on the Bioanalyzer gel image B) Chromatogram representation of panel A showing high quality RRBS libraries.

    Article Snippet: AMPure SPRI bead-purified library was eluted in 60 μL of dH2 O. Purified libraries can be screened on an Agilent 2100 BioAnalyzer (Figure A and B).

    Techniques: Chromatin Immunoprecipitation

    Rapid PCR screening of DM1 with Agilent bioanalyzer detection. 10 ng purified donor DNA (P) or 10% direct whole blood (B) from 40 numerically-indicated donors with detection using the Agilent 2100 Bioanalyzer DNA 1000 kit. N (no template control); M (marker; the 200 to 100 base pair range is indicated).

    Journal: BMC Medical Genetics

    Article Title: PCR amplification of a triple-repeat genetic target directly from whole blood in 15 minutes as a proof-of-principle PCR study for direct sample analysis for a clinically relevant target

    doi: 10.1186/s12881-014-0130-5

    Figure Lengend Snippet: Rapid PCR screening of DM1 with Agilent bioanalyzer detection. 10 ng purified donor DNA (P) or 10% direct whole blood (B) from 40 numerically-indicated donors with detection using the Agilent 2100 Bioanalyzer DNA 1000 kit. N (no template control); M (marker; the 200 to 100 base pair range is indicated).

    Article Snippet: PCR products retained in the supernatant (after a 20-second pulse on a picofuge to separate out cellular debris) were resolved by agarose gel electrophoresis and an Agilent 2100 Bioanalyzer (Agilent Technologies, Loveland, CO USA).

    Techniques: Polymerase Chain Reaction, Purification, Marker

    DM1 screening using the Gene Link Genemer Kit with Agilent bioanalyzer detection. 100 ng purified donor DNA (P) from 40 numerically-indicated donors with detection using the Agilent 2100 Bioanalyzer DNA 1000 kit. N (no template control); M (marker; the 200 to 100 base pair range is indicated).

    Journal: BMC Medical Genetics

    Article Title: PCR amplification of a triple-repeat genetic target directly from whole blood in 15 minutes as a proof-of-principle PCR study for direct sample analysis for a clinically relevant target

    doi: 10.1186/s12881-014-0130-5

    Figure Lengend Snippet: DM1 screening using the Gene Link Genemer Kit with Agilent bioanalyzer detection. 100 ng purified donor DNA (P) from 40 numerically-indicated donors with detection using the Agilent 2100 Bioanalyzer DNA 1000 kit. N (no template control); M (marker; the 200 to 100 base pair range is indicated).

    Article Snippet: PCR products retained in the supernatant (after a 20-second pulse on a picofuge to separate out cellular debris) were resolved by agarose gel electrophoresis and an Agilent 2100 Bioanalyzer (Agilent Technologies, Loveland, CO USA).

    Techniques: Purification, Marker

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.

    Journal: Scientific Reports

    Article Title: A single-step method for RNA isolation from tropical crops in the field

    doi: 10.1038/srep38368

    Figure Lengend Snippet: Depicts the integrity of RNA isolated at 30 °C from different Coffea arabica tissues (mature and immature leaf, fruit, root) and from leaves belonging to 3 different coffee species, Coffea arabica, C. canephora and C. eugenoides . The isolated RNA were analysed by electrophoretic separation using 2% agarose gel ( A ) and the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits ( B ). The total RNA fraction contained ribosomal RNA, mRNA and small RNA had a RIN value ranging between 7.5 and 8.5.

    Article Snippet: The Agilent 2100 bioanalyser system confirmed the absence of genomic DNA in the RNA samples.

    Techniques: Isolation, Agarose Gel Electrophoresis

    Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.

    Journal: Scientific Reports

    Article Title: A single-step method for RNA isolation from tropical crops in the field

    doi: 10.1038/srep38368

    Figure Lengend Snippet: Comparison of the effectiveness of RNAzol RT (RNA extraction in field) and TRIzol Reagent or spin-columns kit (RNA extraction in laboratory with recommended cold conditions) to isolate total RNA. ( A ) Electrophoretic separation using 2% agarose gel of RNA extracted from the same four samples using the three methods. ( B ) Electropherogram and Electrophoresis of representative sample of RNA analysed with the Agilent 2100 bioanalyser system with the RNA 6000 Nano ™ kits. The white arrow shows genomic DNA contaminants.

    Article Snippet: The Agilent 2100 bioanalyser system confirmed the absence of genomic DNA in the RNA samples.

    Techniques: RNA Extraction, Agarose Gel Electrophoresis, Electrophoresis

    Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.

    Journal: Scientific Reports

    Article Title: A single-step method for RNA isolation from tropical crops in the field

    doi: 10.1038/srep38368

    Figure Lengend Snippet: Depicts the integrity of RNA isolated at 30 °C from leaf tissue samples from Coffea arabica, Manihot esculenta, Oryza sativa and Zea mais . ( A ) The left-hand side of the figure shows the electrophoresis analysis by agarose gel. ( B ) The right-hand side of the figure shows the Agilent 2100 bioanalyser electrophoresis for each sample. The total RNA fraction had a RIN value ranging between 8 and 9.

    Article Snippet: The Agilent 2100 bioanalyser system confirmed the absence of genomic DNA in the RNA samples.

    Techniques: Isolation, Electrophoresis, Agarose Gel Electrophoresis