2100 bioanalyzer Search Results


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  • 87
    Agilent technologies 2100 bioanalyzer bioa
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    2100 Bioanalyzer Bioa, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 87/100, based on 2298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer bioa/product/Agilent technologies
    Average 87 stars, based on 2298 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer bioa - by Bioz Stars, 2020-02
    87/100 stars
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    92
    Thermo Fisher bioanalyzer 2100
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Bioanalyzer 2100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer 2100/product/Thermo Fisher
    Average 92 stars, based on 439 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100 - by Bioz Stars, 2020-02
    92/100 stars
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    88
    Stratagene bioanalyzer 2100
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Bioanalyzer 2100, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer 2100/product/Stratagene
    Average 88 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100 - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    92
    Biogen Inc agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by Biogen Inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Biogen Inc
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    94
    Thermo Fisher agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Thermo Fisher
    Average 94 stars, based on 376 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    92
    Bio-Rad agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Bio-Rad
    Average 92 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    90
    Hewlett-Packard agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Hewlett-Packard
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    89
    Hewlett-Packard agilent bioanalyzer 2100
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent Bioanalyzer 2100, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 89/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent bioanalyzer 2100/product/Hewlett-Packard
    Average 89 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    agilent bioanalyzer 2100 - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    94
    Illumina Inc agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Illumina Inc
    Average 94 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    94/100 stars
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    78
    Illumina Inc hiseq 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Hiseq 2100 Bioanalyzer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2100 bioanalyzer/product/Illumina Inc
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hiseq 2100 bioanalyzer - by Bioz Stars, 2020-02
    78/100 stars
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    93
    Illumina Inc agilent bioanalyzer 2100
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent Bioanalyzer 2100, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent bioanalyzer 2100/product/Illumina Inc
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    agilent bioanalyzer 2100 - by Bioz Stars, 2020-02
    93/100 stars
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    90
    Santa Cruz Biotechnology agilent bioanalyzer 2100
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent Bioanalyzer 2100, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent bioanalyzer 2100/product/Santa Cruz Biotechnology
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    agilent bioanalyzer 2100 - by Bioz Stars, 2020-02
    90/100 stars
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    94
    CD Genomics agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by CD Genomics, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/CD Genomics
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    88
    Miltenyi Biotec 2100 bioanalyzer system
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    2100 Bioanalyzer System, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer system/product/Miltenyi Biotec
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer system - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    89
    Roche agilent bioanalyzer 2100
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent Bioanalyzer 2100, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent bioanalyzer 2100/product/Roche
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    agilent bioanalyzer 2100 - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    90
    GATC Biotech agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/GATC Biotech
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Stratagene agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Stratagene
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    88
    GE Healthcare 2100 bioanalyzer system
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    2100 Bioanalyzer System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer system/product/GE Healthcare
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer system - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    89
    Shanghai Biochip Co. Ltd agilent bioanalyzer 2100
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent Bioanalyzer 2100, supplied by Shanghai Biochip Co. Ltd, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent bioanalyzer 2100/product/Shanghai Biochip Co. Ltd
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    agilent bioanalyzer 2100 - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    94
    Caliper Life Sciences agilent 2100 bioanalyzer
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Agilent 2100 Bioanalyzer, supplied by Caliper Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Caliper Life Sciences
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    88
    Thermo Fisher bioanalyzer 2100 system
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    Bioanalyzer 2100 System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer 2100 system/product/Thermo Fisher
    Average 88 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100 system - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    Image Search Results


    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

    Journal: BMC Research Notes

    Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

    doi: 10.1186/1756-0500-4-217

    Figure Lengend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

    Article Snippet: The Small RNA Kit (Agilent) was used to analyse smallRNA and miRNA content with the 2100 Bioanalyzer (Agilent).

    Techniques: Software, Electrophoresis, Standard Deviation

    Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).

    Journal: Scientific Reports

    Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy

    doi: 10.1038/s41598-019-39111-7

    Figure Lengend Snippet: Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).

    Article Snippet: The TE transcriptome obtained with PGT-AT was of high quality All tested samples produced sufficient quantity and high-quality cDNA for RNAseq, as per BioAnalyzer 2100 (Agilent Technologies, CA) (see Supplementary Fig. ), enabling us to conduct gene expression profiling.

    Techniques: Lysis, Amplification, Synthesized, Produced, Next-Generation Sequencing

    Appearance of DNA libraries from Agilent 2100 Bioanalyzer analysis. Representative electropherogram ( a ) and virtual gel ( b ) used for visual inspection (generated with the Agilent 2100 Bioanalyzer system with the DNA 12000 Kit) of DNA libraries sizes prepared for sequencing with the PacBio SMRTbell 10 kb Template Preparation Kit on the Agilent NGS Workstation. A typical electropherogram using the Agilent bioanalyzer 2100 DNA 12000 kit shows the lower marker at 35 s and the upper marker at 90 s. The DNA libraries and the upper marker co-elutes with each other, the sharper peak is the upper marker, shown in red on the gel image. The average library sizes are: Campylobacter ( green , 9.1 kb), Listeria ( blue , 9.5 kb), Vibrio ( aqua , 10 kb), and Salmonella ( red , 15 kb)

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: Appearance of DNA libraries from Agilent 2100 Bioanalyzer analysis. Representative electropherogram ( a ) and virtual gel ( b ) used for visual inspection (generated with the Agilent 2100 Bioanalyzer system with the DNA 12000 Kit) of DNA libraries sizes prepared for sequencing with the PacBio SMRTbell 10 kb Template Preparation Kit on the Agilent NGS Workstation. A typical electropherogram using the Agilent bioanalyzer 2100 DNA 12000 kit shows the lower marker at 35 s and the upper marker at 90 s. The DNA libraries and the upper marker co-elutes with each other, the sharper peak is the upper marker, shown in red on the gel image. The average library sizes are: Campylobacter ( green , 9.1 kb), Listeria ( blue , 9.5 kb), Vibrio ( aqua , 10 kb), and Salmonella ( red , 15 kb)

    Article Snippet: We will discuss the automation of preparation of libraries with the SMRTbell Template Preparation kit as well as analysis of gDNA, fragmented DNA and the final libraries ready for sequencing with both the Agilent electrophoresis platform: Agilent 2100 Bioanalyzer System using the DNA 12000 assay and the Agilent TapeStation System using the genomic DNA ScreenTape and matching reagents.

    Techniques: Generated, Sequencing, Next-Generation Sequencing, Marker

    Appearance of sheared DNA from Agilent 2100 Bioanalyzer analysis. Representative electropherogram ( a ) and virtual gel ( b ) are used for visual inspection (generated with the Agilent 2100 Bioanalyzer system with the DNA 12000 Kit) of sheared bacterial genomic DNA with average shearing size for Campylobacter ( green , 10 kb), Listeria ( blue , 13.5 kb), Vibrio ( aqua ,11.6 kb), and Salmonella ( red , 17 kb). Peaks near 35 are the lower marker internal standard for the DNA 12000 kit. A typical electropherogram using the Agilent Bioanalyzer 2100 DNA 12000 kit shows the lower marker at 35 s and the upper marker at 90 s. The sheared DNA and the red upper marker, seen in the gel image, co-elute together

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: Appearance of sheared DNA from Agilent 2100 Bioanalyzer analysis. Representative electropherogram ( a ) and virtual gel ( b ) are used for visual inspection (generated with the Agilent 2100 Bioanalyzer system with the DNA 12000 Kit) of sheared bacterial genomic DNA with average shearing size for Campylobacter ( green , 10 kb), Listeria ( blue , 13.5 kb), Vibrio ( aqua ,11.6 kb), and Salmonella ( red , 17 kb). Peaks near 35 are the lower marker internal standard for the DNA 12000 kit. A typical electropherogram using the Agilent Bioanalyzer 2100 DNA 12000 kit shows the lower marker at 35 s and the upper marker at 90 s. The sheared DNA and the red upper marker, seen in the gel image, co-elute together

    Article Snippet: We will discuss the automation of preparation of libraries with the SMRTbell Template Preparation kit as well as analysis of gDNA, fragmented DNA and the final libraries ready for sequencing with both the Agilent electrophoresis platform: Agilent 2100 Bioanalyzer System using the DNA 12000 assay and the Agilent TapeStation System using the genomic DNA ScreenTape and matching reagents.

    Techniques: Generated, Marker

    5-Aza-CdR treatment in vivo leads to inhibition of DNMT1 and apoptosis and results in demethylation of tumor suppressor p16INK4A. A. Cell cycle analysis shows that 5-aza-CdR treatment leads to increased apoptosis. Tumor samples were prepared for FACS analysis as described in the methods section and analyzed with a BD FACSCanto II flow cytometer using the BD FACS Diva Software. Values are means ± SEM. Each value is the mean of three replicates for control and 5-aza-CdR day 5 tumors and of two replicates for 5-aza-CdR day 3 tumors. B. DNMT1 is not detected in tumor protein extracts derived from 5-aza-CdR treated mice. Proteins were extracted from tumor tissue from two tumors treated with 5-aza-CdR starting on day 3 and three tumors treated with 5-aza-CdR on day 5 as described in materials and methods. DNMT1 protein levels were analysed by Western Blot. C. Analysis of the p16 INK4A promotor region by COBRA shows demethylation after 5-aza-CdR treatment. DNA was extracted from tumor tissue, bisulfite converted and analysed by COBRA. Restriction fragments were analysed using the Agilent 2100 Bioanalyzer platform. Normal PBMCs and in vitro by M.SssI methylated PBMCs were used as unmethylated and methylated controls, respectively. Note the re-appearance of the unmethylated fragment in treated samples indicated by the arrow. D. The percentage of methylated fragments is significantly reduced in 5-aza-CdR treated tumors. Percentages of methylated and unmethylated fragments in relation to total peak areas in electropherograms were calculated with Agilent software as described in the methods section.

    Journal: Biochimie

    Article Title: Antineoplastic activity of the DNA methyltransferase inhibitor 5-aza-2?-deoxycytidine in anaplastic large cell lymphoma

    doi: 10.1016/j.biochi.2012.05.029

    Figure Lengend Snippet: 5-Aza-CdR treatment in vivo leads to inhibition of DNMT1 and apoptosis and results in demethylation of tumor suppressor p16INK4A. A. Cell cycle analysis shows that 5-aza-CdR treatment leads to increased apoptosis. Tumor samples were prepared for FACS analysis as described in the methods section and analyzed with a BD FACSCanto II flow cytometer using the BD FACS Diva Software. Values are means ± SEM. Each value is the mean of three replicates for control and 5-aza-CdR day 5 tumors and of two replicates for 5-aza-CdR day 3 tumors. B. DNMT1 is not detected in tumor protein extracts derived from 5-aza-CdR treated mice. Proteins were extracted from tumor tissue from two tumors treated with 5-aza-CdR starting on day 3 and three tumors treated with 5-aza-CdR on day 5 as described in materials and methods. DNMT1 protein levels were analysed by Western Blot. C. Analysis of the p16 INK4A promotor region by COBRA shows demethylation after 5-aza-CdR treatment. DNA was extracted from tumor tissue, bisulfite converted and analysed by COBRA. Restriction fragments were analysed using the Agilent 2100 Bioanalyzer platform. Normal PBMCs and in vitro by M.SssI methylated PBMCs were used as unmethylated and methylated controls, respectively. Note the re-appearance of the unmethylated fragment in treated samples indicated by the arrow. D. The percentage of methylated fragments is significantly reduced in 5-aza-CdR treated tumors. Percentages of methylated and unmethylated fragments in relation to total peak areas in electropherograms were calculated with Agilent software as described in the methods section.

    Article Snippet: Restriction fragments were analyzed on an Agilent 2100 Bioanalyzer platform using the Agilent DNA chip 1000 series.

    Techniques: In Vivo, Inhibition, Cell Cycle Assay, FACS, Flow Cytometry, Cytometry, Software, Derivative Assay, Mouse Assay, Western Blot, Combined Bisulfite Restriction Analysis Assay, In Vitro, Methylation

    5-Aza-CdR treatment leads to demethylation and re-expression of the tumor suppressor p16 INK4A and induces cellular senescence. A. p16 INK4A promoter methylation decreases upon treatment with increasing 5-aza-CdR concentrations. 1 × 10 6 KARPAS-299 cells were incubated with 0, 1 and 10 μM of 5-aza-CdR, the medium was changed after 24 h and then cells were grown for 4 days. DNA was extracted from cells and bisulfite converted and Combined Bisulfite Restriction Analysis (COBRA) was performed to analyse the methylation status of the p16 INK4A promoter as described in materials and methods. Restriction fragments were analysed using the Agilent 2100 Bioanalyzer platform. Note the dose-dependent increase of the unmethylated fragment at 220 bp indicated by the arrow. B. p16 INK4A mRNA increases in the 5-aza-CdR treated cell line KARPAS-299 compared to mock treated controls. RNA was isolated from 1 μM 5-aza-CdR treated KARPAS-299 cells as described in A. p16 INK4A expression was analysed by quantitative RT–PCR. Values are means ± SEM. Each value is the mean of three replicates. Data were analysed by unpaired t -tests. C. Senescent cells accumulate upon 5-aza-CdR treatment. 5-Aza-CdR treated KARPAS-299 and control cells were stained for β-galactosidase activity and counterstained with nuclear fast red. Note the abnormal enlarged shape of 5-aza-CdR treated cells.

    Journal: Biochimie

    Article Title: Antineoplastic activity of the DNA methyltransferase inhibitor 5-aza-2?-deoxycytidine in anaplastic large cell lymphoma

    doi: 10.1016/j.biochi.2012.05.029

    Figure Lengend Snippet: 5-Aza-CdR treatment leads to demethylation and re-expression of the tumor suppressor p16 INK4A and induces cellular senescence. A. p16 INK4A promoter methylation decreases upon treatment with increasing 5-aza-CdR concentrations. 1 × 10 6 KARPAS-299 cells were incubated with 0, 1 and 10 μM of 5-aza-CdR, the medium was changed after 24 h and then cells were grown for 4 days. DNA was extracted from cells and bisulfite converted and Combined Bisulfite Restriction Analysis (COBRA) was performed to analyse the methylation status of the p16 INK4A promoter as described in materials and methods. Restriction fragments were analysed using the Agilent 2100 Bioanalyzer platform. Note the dose-dependent increase of the unmethylated fragment at 220 bp indicated by the arrow. B. p16 INK4A mRNA increases in the 5-aza-CdR treated cell line KARPAS-299 compared to mock treated controls. RNA was isolated from 1 μM 5-aza-CdR treated KARPAS-299 cells as described in A. p16 INK4A expression was analysed by quantitative RT–PCR. Values are means ± SEM. Each value is the mean of three replicates. Data were analysed by unpaired t -tests. C. Senescent cells accumulate upon 5-aza-CdR treatment. 5-Aza-CdR treated KARPAS-299 and control cells were stained for β-galactosidase activity and counterstained with nuclear fast red. Note the abnormal enlarged shape of 5-aza-CdR treated cells.

    Article Snippet: Restriction fragments were analyzed on an Agilent 2100 Bioanalyzer platform using the Agilent DNA chip 1000 series.

    Techniques: Expressing, Methylation, Incubation, Combined Bisulfite Restriction Analysis Assay, Isolation, Quantitative RT-PCR, Staining, Activity Assay

    Comparison of small RNA extraction from MDA-MB-231 cells with fibers and columns analyzed by an Agilent 2100 Bioanalyzer. Small RNA recovery was as high as 985 pg/μL with the fibers and 10.2 pg/μL with the columns from as little as 134,000

    Journal: Analytical and bioanalytical chemistry

    Article Title: Extraction of microRNAs from biological matrices with titanium dioxide nanofibers

    doi: 10.1007/s00216-017-0649-3

    Figure Lengend Snippet: Comparison of small RNA extraction from MDA-MB-231 cells with fibers and columns analyzed by an Agilent 2100 Bioanalyzer. Small RNA recovery was as high as 985 pg/μL with the fibers and 10.2 pg/μL with the columns from as little as 134,000

    Article Snippet: The chip was run on an Agilent 2100 Bioanalyzer Instrument using the Small RNA Analysis Kit with 1 μL of extraction sample following the protocol provided by Agilent.

    Techniques: RNA Extraction, Multiple Displacement Amplification

    Agilent Bioanalyzer 2100 analyses (with a DNA 7500 chip) of PCR products. (Upper panels) Gel view of Agilent traces. Bands representing dup + and dup − genomes are marked by arrows labeled accordingly. (Lower panels) Bar plots of relative quantities

    Journal: Journal of Virology

    Article Title: Mixed Triple: Allied Viruses in Unique Recent Isolates of Highly Virulent Type 2 Bovine Viral Diarrhea Virus Detected by Deep Sequencing

    doi: 10.1128/JVI.00620-14

    Figure Lengend Snippet: Agilent Bioanalyzer 2100 analyses (with a DNA 7500 chip) of PCR products. (Upper panels) Gel view of Agilent traces. Bands representing dup + and dup − genomes are marked by arrows labeled accordingly. (Lower panels) Bar plots of relative quantities

    Article Snippet: PCR products were analyzed with a Bioanalyzer 2100 instrument (Agilent Technologies, Böblingen, Germany), using an Agilent DNA 7500 chip.

    Techniques: Chromatin Immunoprecipitation, Polymerase Chain Reaction, Labeling

    Comparison of total RNA and microarray signals between FNAB and FFPE specimens . (A) Comparison of Agilent 2100 Bioanalyzer analysis of total RNA between FNAB and FFPE from a breast cancer sample E(+)H(-)-2. (B) Noise levels of the microarray signals. The average numbers of under-detectable probes from Illumina Human-Ref8 24 K BeadChip are 7906.8 ± 27.9 in 25 FNAB arrays and 9531.4 ± 74.3 in 25 FFPE arrays, respectively. (C) Reproducibility of microarray signal. The averages of Correlation Coefficients are 0.87 ± 0.04 within 25 FNAB arrays, 0.87 ± 0.08 within 25 FFPE arrays, 0.45 ± 0.02 between the 25 FNAB arrays and 25 FFPE arrays, and 0.47 ± 0.02 between the 25 paired FNAB and FFPE arrays, respectively.

    Journal: BMC Cancer

    Article Title: Clinical relevance of DNA microarray analyses using archival formalin-fixed paraffin-embedded breast cancer specimens

    doi: 10.1186/1471-2407-11-253

    Figure Lengend Snippet: Comparison of total RNA and microarray signals between FNAB and FFPE specimens . (A) Comparison of Agilent 2100 Bioanalyzer analysis of total RNA between FNAB and FFPE from a breast cancer sample E(+)H(-)-2. (B) Noise levels of the microarray signals. The average numbers of under-detectable probes from Illumina Human-Ref8 24 K BeadChip are 7906.8 ± 27.9 in 25 FNAB arrays and 9531.4 ± 74.3 in 25 FFPE arrays, respectively. (C) Reproducibility of microarray signal. The averages of Correlation Coefficients are 0.87 ± 0.04 within 25 FNAB arrays, 0.87 ± 0.08 within 25 FFPE arrays, 0.45 ± 0.02 between the 25 FNAB arrays and 25 FFPE arrays, and 0.47 ± 0.02 between the 25 paired FNAB and FFPE arrays, respectively.

    Article Snippet: As expected, the landmark ribosomal peaks (18S and 28S) were not well detected in the FFPE samples using Agilent 2100 Bioanalyzer (Figure ).

    Techniques: Microarray, Formalin-fixed Paraffin-Embedded

    Quality check of IP specificity and first and second PCR-sample sizes and amounts used for NMD-DegSeq. (A) Western blot of lysates of HEK293T cells using the specified antibodies prior to (Input) or after IP using anti(α)-p-UFP1 S1116 antibody (i.e. (α)-p-UFPl) or, as a negative control, rabbit (r)IgG. The left-most five lanes are 3-fold dilutions of Input sample (i.e. before IP). (B) SYBR Gold staining after the second PCR-amplification for the purpose of quality control. Analyses are of RNA samples prior to (Input); after (+) IP using anti(α)-p-UFP1 S1116 antibody or, as a negative control, rabbit (r)IgG; or no RNA (None). These RNAs were subjected to the first PCR and, subsequently, as second PCR, after which PCR products were (+) or were not (−) size-selected (the latter has Adapter self-ligation products that should be eliminated prior to library construction). (C) Agilent Bioanalyzer 2100 analyses using capillary electrophoresis (upper) and tracing (lower) of fluorescent samples after the first PCR. Results confirm adequate quality and quantity of samples before (Input) and after IP so that index PCR can be performed to generate samples for Illumina sequencing. FU, fluorescence units; bp, base pairs.

    Journal: Methods (San Diego, Calif.)

    Article Title: Defining nonsense-mediated mRNA decay intermediates in human cells

    doi: 10.1016/j.ymeth.2018.12.005

    Figure Lengend Snippet: Quality check of IP specificity and first and second PCR-sample sizes and amounts used for NMD-DegSeq. (A) Western blot of lysates of HEK293T cells using the specified antibodies prior to (Input) or after IP using anti(α)-p-UFP1 S1116 antibody (i.e. (α)-p-UFPl) or, as a negative control, rabbit (r)IgG. The left-most five lanes are 3-fold dilutions of Input sample (i.e. before IP). (B) SYBR Gold staining after the second PCR-amplification for the purpose of quality control. Analyses are of RNA samples prior to (Input); after (+) IP using anti(α)-p-UFP1 S1116 antibody or, as a negative control, rabbit (r)IgG; or no RNA (None). These RNAs were subjected to the first PCR and, subsequently, as second PCR, after which PCR products were (+) or were not (−) size-selected (the latter has Adapter self-ligation products that should be eliminated prior to library construction). (C) Agilent Bioanalyzer 2100 analyses using capillary electrophoresis (upper) and tracing (lower) of fluorescent samples after the first PCR. Results confirm adequate quality and quantity of samples before (Input) and after IP so that index PCR can be performed to generate samples for Illumina sequencing. FU, fluorescence units; bp, base pairs.

    Article Snippet: Indexed libraries are purified using Agencourt AMPure XP SPRI beads followed by quality assessment and quantification using an Agilent Bioanalyzer 2100 and Qubit 3.0 Flourometer, respectively.

    Techniques: Polymerase Chain Reaction, Western Blot, Negative Control, Staining, Amplification, Ligation, Electrophoresis, Sequencing, Fluorescence

    Agilent Bioanalyzer 2100 analysis of RNA from protein interaction profiling. The bioanalyzer traces from a structure only (top) and a footprinting (bottom) sample. The arrow indicates the longer RNA fragments in the footprinting sample.

    Journal: Current protocols in molecular biology

    Article Title: Protein Interaction Profile Sequencing (PIP-seq)

    doi: 10.1002/cpmb.21

    Figure Lengend Snippet: Agilent Bioanalyzer 2100 analysis of RNA from protein interaction profiling. The bioanalyzer traces from a structure only (top) and a footprinting (bottom) sample. The arrow indicates the longer RNA fragments in the footprinting sample.

    Article Snippet: Agilent Bioanalyzer 2100 analysis of RNA from protein interaction profiling should reveal RNA fragments from ~15 nt to ~1000 nt.

    Techniques: Footprinting

    Experimental design for the Bioanalyzer 2100 to identify site-specific endonuclease inhibition by opioid compounds, HindIII, EcoRI, BamHI and SalI.

    Journal: Nucleic Acids Research

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents

    doi: 10.1093/nar/gkw1097

    Figure Lengend Snippet: Experimental design for the Bioanalyzer 2100 to identify site-specific endonuclease inhibition by opioid compounds, HindIII, EcoRI, BamHI and SalI.

    Article Snippet: The reactions of EcoRI, BamHI, SalI and HindIII, in the presence and absence of tripodal scaffolds, were then examined using the Agilent DNA 1000 microfluidic chip (Agilent 5067-1504) with data being collected on the Agilent Bioanalyzer 2100 platform.

    Techniques: Inhibition

    ( A ) Electrograms generated using the Bioanalyzer 2100 of 742 bp dsDNA fragment with treatment by endonucleases BamHI, HindIII, SalI and EcoRI. Electrograms of the 742 bp fragment were pre-incubated for 5 h with either ( B ) MC3 , ( C ) HC3 and ( D ) OC3 , followed by exposure over night to the type II restriction endonuclease.

    Journal: Nucleic Acids Research

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents

    doi: 10.1093/nar/gkw1097

    Figure Lengend Snippet: ( A ) Electrograms generated using the Bioanalyzer 2100 of 742 bp dsDNA fragment with treatment by endonucleases BamHI, HindIII, SalI and EcoRI. Electrograms of the 742 bp fragment were pre-incubated for 5 h with either ( B ) MC3 , ( C ) HC3 and ( D ) OC3 , followed by exposure over night to the type II restriction endonuclease.

    Article Snippet: The reactions of EcoRI, BamHI, SalI and HindIII, in the presence and absence of tripodal scaffolds, were then examined using the Agilent DNA 1000 microfluidic chip (Agilent 5067-1504) with data being collected on the Agilent Bioanalyzer 2100 platform.

    Techniques: Generated, Incubation

    Size distribution of mRNA, cRNA and dsDNA on Agilent 2100 Bioanalyzer 6000 Nanochips. ( a ) Universal Human Reference (UHR) RNA and sense-RNA template library after IVT-amplification. Lane L displays the ladder (25, 200, 500, 1000, 2000 and 4000 nt). Lane 1 contains fresh UHR RNA. Lanes 2, 3 and 4 display three individual IVT-amplifications of sense-RNA using total RNA displayed in lane 1. ( b ) Size distribution of fresh, frozen, FFPE-RNA and amplified cRNA. Lane L displays the same ladder as observed in (a). Lane 1 contains fresh human breast RNA. Lane 2 contains total RNA from the 10-year-old frozen human breast cancer tissue. Lane 3 contains total RNA from the matched 10-year-old FFPE human breast cancer tissue. Lane 4 contains cRNA obtained by IVT-amplifications of 10-year-old frozen RNA (lane 2). Lanes 5 contains cRNA obtained by direct IVT-amplification of the 10-year-old FFPE-RNA (lane 3). Lanes 6 contains amplified cRNA obtained by CT-RT and IVT-amplification of the same 10-year-old FFPE-RNA. ( c ) Size distribution of double-stranded DNA on a Bioanalyzer 2100 Agilent nanochip. Lane L displays the ladder. Lane 1 displays dsDNA obtained from 10-year-old frozen RNA. Lane 2 displays dsDNA obtained from 10-year-old FFPE-RNA. Lane 3 shows dsDNA obtained after CT-RT and double-strand DNA synthesis of the same 10-year-old FFPE-RNA. (See Supplementary Data for fragmented RNA profiles)

    Journal: Nucleic Acids Research

    Article Title: Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)

    doi: 10.1093/nar/gkm510

    Figure Lengend Snippet: Size distribution of mRNA, cRNA and dsDNA on Agilent 2100 Bioanalyzer 6000 Nanochips. ( a ) Universal Human Reference (UHR) RNA and sense-RNA template library after IVT-amplification. Lane L displays the ladder (25, 200, 500, 1000, 2000 and 4000 nt). Lane 1 contains fresh UHR RNA. Lanes 2, 3 and 4 display three individual IVT-amplifications of sense-RNA using total RNA displayed in lane 1. ( b ) Size distribution of fresh, frozen, FFPE-RNA and amplified cRNA. Lane L displays the same ladder as observed in (a). Lane 1 contains fresh human breast RNA. Lane 2 contains total RNA from the 10-year-old frozen human breast cancer tissue. Lane 3 contains total RNA from the matched 10-year-old FFPE human breast cancer tissue. Lane 4 contains cRNA obtained by IVT-amplifications of 10-year-old frozen RNA (lane 2). Lanes 5 contains cRNA obtained by direct IVT-amplification of the 10-year-old FFPE-RNA (lane 3). Lanes 6 contains amplified cRNA obtained by CT-RT and IVT-amplification of the same 10-year-old FFPE-RNA. ( c ) Size distribution of double-stranded DNA on a Bioanalyzer 2100 Agilent nanochip. Lane L displays the ladder. Lane 1 displays dsDNA obtained from 10-year-old frozen RNA. Lane 2 displays dsDNA obtained from 10-year-old FFPE-RNA. Lane 3 shows dsDNA obtained after CT-RT and double-strand DNA synthesis of the same 10-year-old FFPE-RNA. (See Supplementary Data for fragmented RNA profiles)

    Article Snippet: Reagents We used universal human reference RNA (UHR; Stratagene), the quality of which was assessed on a Bioanalyzer 2100 expert (Agilent).

    Techniques: Amplification, Formalin-fixed Paraffin-Embedded, DNA Synthesis

    Fluorescence of total RNA by gel electrophoresis using a Bioanalyzer 2100 nanochip (Agilent Technologies) from two plaque specimens (A and B), human aortic endothelial cells in culture (C), and surgically collected human aorta (D). Reproducible 5S, 18S,

    Journal: Clinical and Translational Science

    Article Title: Transcriptional Regulation of β2‐Microglobulin Demonstrated Via a Novel Genomic and Proteomic Analysis of Percutaneously Collected Peripheral Atheroma

    doi: 10.1111/j.1752-8062.2008.00071.x

    Figure Lengend Snippet: Fluorescence of total RNA by gel electrophoresis using a Bioanalyzer 2100 nanochip (Agilent Technologies) from two plaque specimens (A and B), human aortic endothelial cells in culture (C), and surgically collected human aorta (D). Reproducible 5S, 18S,

    Article Snippet: Denaturing gel analysis using a Bioanalyzer 2100 nanochip (Agilent Technologies) demonstrated similar signatures between plaque, surgical, and cell culture specimens, indicating similar molecular integrity at the mRNA level ( ).

    Techniques: Fluorescence, Nucleic Acid Electrophoresis

    Assessment of total riboneucleic acid (RNA) quality for adipose tissue. Total RNA from adipose tissue extracted using Stat-60 (3A and 4A) and Trizol plus RNeasy Lipid Kit (3B and 4B) were analyzed by capillary electrophoresis (Agilent 2100 Bioanalyzer). Shown for each sample is the electropherogram indicating florescent intensity (y-axis) vs. time (x-axis). In samples 3B and 4B the 2 major peaks correspond to the 18s (at ~ 42 sec) and the 28s (at ~ 48 sec). Lack of the 18s and 28s peaks in samples 3A and 4A indicate degradation of RNA. The corresponding computer generated gel from the electropherogram is shown for each sample. The two dark bands correspond to the 18s and 28s rRNA.

    Journal: Research in nursing & health

    Article Title: Collection and storage of human blood and adipose for genomic analysis of clinical samples

    doi: 10.1002/nur.20448

    Figure Lengend Snippet: Assessment of total riboneucleic acid (RNA) quality for adipose tissue. Total RNA from adipose tissue extracted using Stat-60 (3A and 4A) and Trizol plus RNeasy Lipid Kit (3B and 4B) were analyzed by capillary electrophoresis (Agilent 2100 Bioanalyzer). Shown for each sample is the electropherogram indicating florescent intensity (y-axis) vs. time (x-axis). In samples 3B and 4B the 2 major peaks correspond to the 18s (at ~ 42 sec) and the 28s (at ~ 48 sec). Lack of the 18s and 28s peaks in samples 3A and 4A indicate degradation of RNA. The corresponding computer generated gel from the electropherogram is shown for each sample. The two dark bands correspond to the 18s and 28s rRNA.

    Article Snippet: NanoDrop® results may not, however, always be consistent with the Agilent 2100 Bioanalyzer results.

    Techniques: Electrophoresis, Size-exclusion Chromatography, Generated